FastQCFastQC Report
Thu 26 May 2016
SRR938327_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938327_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2775113
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127740.4603055803493407No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102210.36830932650310094No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76380.2752320355963883No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG29250.10540111339610315No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68050.050.4919551
GTATCAA121050.041.379751
ATCAACG158950.031.1696683
TATCAAC162750.030.6021772
TCAACGC163450.030.3115274
CAACGCA165000.029.9979935
AACGCAG170150.029.162226
GTACATG141100.026.0354161
ACGCAGA194550.025.4315457
TACATGG142950.025.2662892
CGCAGAG196150.025.127278
CGTATAG1751.4805119E-724.4408591
ACATGGG147950.023.5661983
GCAGAGT210750.023.0711179
GAGTACT109500.022.76572212-13
AGTACTT112250.022.7367512-13
GTAAGGT9500.021.5007324
AGAGTAC185800.021.4413710-11
GTACTTT116400.021.27343214-15
CATGGGA83250.021.0547734
Produced by FastQC (version 0.11.5)