##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938327_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2775113 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8174193267085 31.0 31.0 34.0 30.0 34.0 2 32.03696714331993 31.0 31.0 34.0 30.0 34.0 3 32.090764231942984 33.0 31.0 34.0 30.0 34.0 4 35.76444779005396 37.0 35.0 37.0 35.0 37.0 5 35.66435456862477 37.0 35.0 37.0 33.0 37.0 6 35.66735552750465 37.0 35.0 37.0 33.0 37.0 7 35.60475951790071 37.0 35.0 37.0 33.0 37.0 8 35.58813677136751 37.0 35.0 37.0 33.0 37.0 9 37.17079845036941 39.0 37.0 39.0 33.0 39.0 10-11 37.068831431368736 39.0 37.0 39.0 33.0 39.0 12-13 36.95753668409178 39.0 37.0 39.0 33.0 39.0 14-15 37.98398389543057 40.0 37.0 41.0 33.0 41.0 16-17 37.90240289314345 40.0 37.0 41.0 33.0 41.0 18-19 38.15201993576478 40.0 37.5 41.0 33.0 41.0 20-21 38.130557386311835 40.0 37.0 41.0 33.0 41.0 22-23 38.01998351058137 40.0 37.0 41.0 33.0 41.0 24-25 37.891530362907744 40.0 37.0 41.0 32.5 41.0 26-27 37.70152746933188 40.0 37.0 41.0 32.0 41.0 28-29 37.58549453661887 39.5 37.0 41.0 32.0 41.0 30-31 37.322398223063345 39.0 36.0 41.0 31.5 41.0 32-33 37.12289445510868 39.0 36.0 41.0 31.0 41.0 34-35 37.01443959218958 39.0 36.0 41.0 31.0 41.0 36-37 36.963474460319276 39.0 35.5 41.0 31.0 41.0 38-39 36.80502757905714 39.0 35.0 41.0 30.0 41.0 40-41 36.6277756977824 39.0 35.0 41.0 30.0 41.0 42-43 36.40674577936106 39.0 35.0 40.0 30.0 41.0 44-45 36.078418068021016 39.0 35.0 40.0 29.5 41.0 46-47 35.65137311525693 38.0 35.0 40.0 28.0 41.0 48-49 35.28083072653257 38.0 34.0 40.0 27.0 41.0 50-51 34.901517163445234 38.0 34.0 40.0 26.0 41.0 52-53 34.42438001623718 38.0 33.0 40.0 24.0 41.0 54-55 34.09001219049459 37.5 33.0 40.0 24.0 41.0 56-57 33.75767275062313 37.0 33.0 40.0 23.0 41.0 58-59 33.29344642902829 36.5 32.0 39.5 22.0 41.0 60-61 32.89605432283298 36.0 31.5 39.0 21.5 40.0 62-63 32.42746980032885 35.5 31.0 39.0 19.5 40.0 64-65 31.904356507284568 35.0 30.5 38.5 18.0 40.0 66-67 31.40544439091309 35.0 30.0 38.0 15.5 40.0 68-69 30.92529367272612 34.5 29.0 37.5 12.5 39.5 70-71 30.3602112778831 34.0 29.0 37.0 8.0 39.0 72-73 29.866307606212793 34.0 28.5 36.0 7.0 39.0 74-75 29.267149662013765 34.0 28.0 36.0 6.0 38.5 76-77 28.049396006577027 31.5 26.5 34.5 2.0 36.5 78-79 28.74188258279933 33.0 28.0 35.0 2.0 37.0 80-81 28.36217191876511 33.0 27.0 35.0 2.0 37.0 82-83 27.924301821223136 33.0 26.0 35.0 2.0 36.0 84-85 27.380232624761586 33.0 26.0 35.0 2.0 36.0 86-87 26.775179605298955 32.0 25.0 35.0 2.0 35.0 88-89 26.051226022147567 32.0 23.5 34.0 2.0 35.0 90-91 25.458346380850077 31.0 21.5 34.0 2.0 35.0 92-93 24.634253992540124 31.0 18.5 34.0 2.0 35.0 94-95 23.828905525648864 31.0 11.0 34.0 2.0 35.0 96-97 22.77700313464713 30.0 2.0 34.0 2.0 35.0 98-99 21.435015619183794 29.5 2.0 34.0 2.0 35.0 100-101 17.54123093365928 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25170764470678364 1101 2 0.3313068424549499 1101 3 0.2699329054922259 1101 4 0.18964827918810556 1101 5 0.1536651683076613 1101 6 0.22354699853395488 1101 7 0.2462978643786613 1101 8 0.22482958125866048 1101 9 0.2932069356326892 1101 10-11 0.31371448633484533 1101 12-13 0.4059869334893591 1101 14-15 0.37601272727882673 1101 16-17 0.47564096703708714 1101 18-19 0.3480246280618289 1101 20-21 0.34400301782787324 1101 22-23 0.37660748697253155 1101 24-25 0.4505233010247167 1101 26-27 0.3819141156609973 1101 28-29 0.30096184005223137 1101 30-31 0.3216829080974506 1101 32-33 0.37824292014388305 1101 34-35 0.4344538135710394 1101 36-37 0.5153784122059264 1101 38-39 0.5425068652368665 1101 40-41 0.5364692822436723 1101 42-43 0.5894759764925581 1101 44-45 0.6137911152225541 1101 46-47 0.6616950888385915 1101 48-49 0.7880299322856743 1101 50-51 0.839646174050344 1101 52-53 0.8600647637117405 1101 54-55 0.8051803937831394 1101 56-57 0.8671931068658196 1101 58-59 0.9293582880188893 1101 60-61 0.9456510384012304 1101 62-63 0.8856211227586996 1101 64-65 0.8475124689593692 1101 66-67 0.7689069264714057 1101 68-69 0.9548338314265976 1101 70-71 0.8977646955336915 1101 72-73 0.9277858399023486 1101 74-75 0.8046182877232688 1101 76-77 0.7541107350785019 1101 78-79 0.9121391247074726 1101 80-81 0.953763754343445 1101 82-83 0.9146323419907425 1101 84-85 0.8743887295605504 1101 86-87 0.8852341914591229 1101 88-89 1.0509596389001352 1101 90-91 0.8706564082611266 1101 92-93 1.1571494109280316 1101 94-95 0.9901690472903653 1101 96-97 1.163761103569957 1101 98-99 1.3299427186800834 1101 100-101 1.434077453498464 1102 1 0.2718934312257346 1102 2 0.25375864488277955 1102 3 0.2853843778573406 1102 4 0.2228189230738593 1102 5 0.14704795981853636 1102 6 0.14288494103571736 1102 7 0.17337581406714975 1102 8 0.15550689775367488 1102 9 0.0918414566794965 1102 10-11 0.3514146737607007 1102 12-13 0.2798217425908973 1102 14-15 0.40719801833778746 1102 16-17 0.38894944686776967 1102 18-19 0.3980631597731019 1102 20-21 0.2986163211847739 1102 22-23 0.4247681009038118 1102 24-25 0.364111638906472 1102 26-27 0.33368551320214124 1102 28-29 0.40388088247516407 1102 30-31 0.40342458535759107 1102 32-33 0.43555256507398354 1102 34-35 0.49300971968100527 1102 36-37 0.5049845105353441 1102 38-39 0.5456377771833019 1102 40-41 0.4576257166003401 1102 42-43 0.518059773267602 1102 44-45 0.7751788276684124 1102 46-47 0.7014325169987217 1102 48-49 0.6974860460204582 1102 50-51 0.7528461942493152 1102 52-53 0.7637780789977384 1102 54-55 0.6490385561022691 1102 56-57 0.658844935347382 1102 58-59 0.8446332390400713 1102 60-61 0.8472122130731989 1102 62-63 1.0194213008901087 1102 64-65 0.9256893957461934 1102 66-67 0.7687975154393989 1102 68-69 0.94173626971914 1102 70-71 0.8840883165338767 1102 72-73 0.8740156235612417 1102 74-75 0.9577070087266044 1102 76-77 0.7947223537250707 1102 78-79 0.9440676057530197 1102 80-81 0.9792841885918087 1102 82-83 0.9969935272012975 1102 84-85 1.1437142672663754 1102 86-87 1.0374197408598533 1102 88-89 1.0168028320336582 1102 90-91 1.0923522133909316 1102 92-93 1.254437041919381 1102 94-95 1.148909890977496 1102 96-97 1.1154210409092684 1102 98-99 1.2929816229011557 1102 100-101 1.4463164130471178 1103 1 0.1643206425264303 1103 2 0.21547820188581568 1103 3 0.19645993652157756 1103 4 0.1576919954327991 1103 5 -0.00442008281713413 1103 6 0.06111114814039098 1103 7 0.11105629189579957 1103 8 0.1145726189352132 1103 9 0.2942420224475697 1103 10-11 0.23340782164940066 1103 12-13 0.18689271531853535 1103 14-15 0.3942566378044674 1103 16-17 0.32751792109197453 1103 18-19 0.4175791262923809 1103 20-21 0.43381853179800345 1103 22-23 0.34810772603310625 1103 24-25 0.3802632455707453 1103 26-27 0.19821198210851065 1103 28-29 0.21037001097094077 1103 30-31 0.08260059746829995 1103 32-33 0.14684895943069165 1103 34-35 0.2794940394302188 1103 36-37 0.2537571872655917 1103 38-39 0.17677046262852514 1103 40-41 0.23319389962038883 1103 42-43 0.3564454869187301 1103 44-45 0.2859723484112351 1103 46-47 0.1319838517784433 1103 48-49 0.19754322920902467 1103 50-51 0.3818522432576259 1103 52-53 0.46564361882889926 1103 54-55 0.36571073425998435 1103 56-57 0.32254841759908714 1103 58-59 0.345996398823722 1103 60-61 0.34923583515930545 1103 62-63 0.4656366692774654 1103 64-65 0.4980716078965237 1103 66-67 0.3884316558477501 1103 68-69 0.43399411724803016 1103 70-71 0.26692729614887867 1103 72-73 0.405136745451685 1103 74-75 0.4642308983961847 1103 76-77 0.25922400329436357 1103 78-79 0.48084351745822573 1103 80-81 0.5891498750826862 1103 82-83 0.6413583037673298 1103 84-85 0.616678394158054 1103 86-87 0.6838289398086985 1103 88-89 0.5825752739673398 1103 90-91 0.8556269274926116 1103 92-93 1.072984419707062 1103 94-95 0.9939965436033731 1103 96-97 1.1926849064223042 1103 98-99 1.361570989084381 1103 100-101 1.4936030319694638 1104 1 0.25310011049479897 1104 2 0.24218019497729415 1104 3 0.21588217243830599 1104 4 0.16639170190182995 1104 5 0.08367366870322712 1104 6 0.12136635250389105 1104 7 0.1434136212505166 1104 8 0.11259574463458222 1104 9 0.25527875976708003 1104 10-11 0.28528360885837145 1104 12-13 0.27634033112271794 1104 14-15 0.3998256175816408 1104 16-17 0.3216762899679608 1104 18-19 0.33576007218646 1104 20-21 0.31615727896675594 1104 22-23 0.38755953127558485 1104 24-25 0.3762571965624275 1104 26-27 0.2942478950546885 1104 28-29 0.2993991559471212 1104 30-31 0.2671690718100166 1104 32-33 0.3232573539838768 1104 34-35 0.32656359491730313 1104 36-37 0.3914211835032049 1104 38-39 0.3544358238121674 1104 40-41 0.36228860254867357 1104 42-43 0.4311977694868787 1104 44-45 0.44650838028340445 1104 46-47 0.38348198139696166 1104 48-49 0.40204653751384 1104 50-51 0.5722302522203222 1104 52-53 0.42782974881500735 1104 54-55 0.5617275920190963 1104 56-57 0.4600500393171316 1104 58-59 0.5584092314786417 1104 60-61 0.5785921752660173 1104 62-63 0.632378137689102 1104 64-65 0.6703806370820509 1104 66-67 0.6345583850046204 1104 68-69 0.7765740138905315 1104 70-71 0.7103564324759049 1104 72-73 0.5954790766173232 1104 74-75 0.7327463912092718 1104 76-77 0.7553083650672221 1104 78-79 0.9353877065721754 1104 80-81 0.8810327708224328 1104 82-83 0.9713476544854807 1104 84-85 0.998408659200841 1104 86-87 1.0705482852832944 1104 88-89 1.0668343380197989 1104 90-91 1.243510058381478 1104 92-93 1.6321386802306606 1104 94-95 1.443026148066341 1104 96-97 1.3754714504870869 1104 98-99 1.559498636133668 1104 100-101 1.8266724306904685 1105 1 0.1855401870486375 1105 2 0.15585763474516767 1105 3 0.16608976299872324 1105 4 0.13012663422267678 1105 5 0.06576801822708234 1105 6 0.02514233990955006 1105 7 0.10267879696936433 1105 8 0.15913461577409294 1105 9 0.26765277776340923 1105 10-11 0.21471748084675824 1105 12-13 0.2745704093743271 1105 14-15 0.3841860997473745 1105 16-17 0.23182758412918503 1105 18-19 0.21776335968009164 1105 20-21 0.23164264730495177 1105 22-23 0.1572597982879742 1105 24-25 0.24613765835496082 1105 26-27 0.04090773206909404 1105 28-29 0.12413040720115731 1105 30-31 0.3527579267435712 1105 32-33 0.384390265962395 1105 34-35 0.3527609062722732 1105 36-37 0.3621320075374044 1105 38-39 0.44592055934641905 1105 40-41 0.40796006926473183 1105 42-43 0.5684135864985009 1105 44-45 0.41734383582507206 1105 46-47 0.39627137344215413 1105 48-49 0.537962212530438 1105 50-51 0.6046666631416002 1105 52-53 0.5800785179341759 1105 54-55 0.6301894540060999 1105 56-57 0.5561442113471955 1105 58-59 0.626617586034051 1105 60-61 0.4830063685953334 1105 62-63 0.487513688923066 1105 64-65 0.6024510635301716 1105 66-67 0.39289654153870757 1105 68-69 0.6304698096029 1105 70-71 0.5210672179245037 1105 72-73 0.6572853074209846 1105 74-75 0.698924757031687 1105 76-77 0.7346365124951078 1105 78-79 0.8027485282912394 1105 80-81 0.7987393195714851 1105 82-83 0.7985674844001309 1105 84-85 0.8367179922872268 1105 86-87 0.7997749525673292 1105 88-89 0.7307398187192753 1105 90-91 0.9113321710205788 1105 92-93 0.9294891995929184 1105 94-95 1.0941086672433897 1105 96-97 1.228413988802597 1105 98-99 1.4015166247431985 1105 100-101 1.5412496867908523 1106 1 -0.048798069149377454 1106 2 0.06410331947478909 1106 3 0.0015326971048494897 1106 4 0.07197646286417125 1106 5 0.016206602094285927 1106 6 -0.019497852662368587 1106 7 0.12314335098024287 1106 8 0.14730413723771107 1106 9 -0.1675607273172588 1106 10-11 0.09945527480348915 1106 12-13 0.13149577260539047 1106 14-15 0.04576383268462081 1106 16-17 0.12005667836550771 1106 18-19 0.10713069910628548 1106 20-21 0.24544815451049118 1106 22-23 0.17855722233048255 1106 24-25 0.20586632514181247 1106 26-27 0.19407395108783732 1106 28-29 0.2715606481028985 1106 30-31 0.15110257625267565 1106 32-33 0.01242154678079288 1106 34-35 0.012028063963008151 1106 36-37 0.1009842362584763 1106 38-39 -0.0751575853865063 1106 40-41 0.030515868716442185 1106 42-43 0.1458693632774697 1106 44-45 0.13229960629202253 1106 46-47 -0.4757994109793984 1106 48-49 -0.29894687580794965 1106 50-51 0.1430883242511669 1106 52-53 0.09271213692393587 1106 54-55 0.2829634445764597 1106 56-57 0.3066832572032112 1106 58-59 0.03098561220719631 1106 60-61 0.11738399912648845 1106 62-63 -0.06241874729656871 1106 64-65 0.10317744991318634 1106 66-67 0.13649186883169406 1106 68-69 -0.12405192669536547 1106 70-71 0.043428721011579796 1106 72-73 0.3039909924232873 1106 74-75 0.4941522192340919 1106 76-77 0.23386146049675105 1106 78-79 0.4832900711793471 1106 80-81 -0.00504552715517903 1106 82-83 0.5834484852934061 1106 84-85 0.5691262875843748 1106 86-87 0.732326997634086 1106 88-89 0.8896552710810077 1106 90-91 0.7503526274952144 1106 92-93 0.7795058128094645 1106 94-95 1.057324342141424 1106 96-97 1.325671937014807 1106 98-99 1.304848534086542 1106 100-101 1.1948790812769836 1107 1 0.10922478788213041 1107 2 0.10369002225543511 1107 3 0.09140827924791495 1107 4 0.08618026652363397 1107 5 0.024039958161239383 1107 6 0.030786642358968663 1107 7 0.10266019659298564 1107 8 0.12149985422362164 1107 9 0.09508419951296077 1107 10-11 0.12602462533642722 1107 12-13 0.16440366247074678 1107 14-15 0.29488964378771954 1107 16-17 0.2460658075379385 1107 18-19 0.2863730362523782 1107 20-21 0.21742539490878698 1107 22-23 0.19071865376912456 1107 24-25 0.15637929304843112 1107 26-27 0.13789738880097246 1107 28-29 0.17939717063840988 1107 30-31 0.21937772775601871 1107 32-33 0.22908423506388687 1107 34-35 0.2406716949768608 1107 36-37 0.2739330953554955 1107 38-39 0.18177600646056646 1107 40-41 0.2801811973471615 1107 42-43 0.1951271917620474 1107 44-45 0.16406099096437288 1107 46-47 0.2029901211626921 1107 48-49 0.1937156858164073 1107 50-51 0.20043404415997657 1107 52-53 0.24188325288488954 1107 54-55 0.18184373257894748 1107 56-57 0.14664920548747062 1107 58-59 0.2603821320146764 1107 60-61 0.22854750581991823 1107 62-63 0.3691733530950998 1107 64-65 0.32441081377452363 1107 66-67 0.29008995141319716 1107 68-69 0.42298060511546964 1107 70-71 0.5610537721622251 1107 72-73 0.5948710758231996 1107 74-75 0.4505000143976794 1107 76-77 0.5585394550017071 1107 78-79 0.7416985066515878 1107 80-81 0.6889467076896132 1107 82-83 0.7064925820217134 1107 84-85 0.883203676075965 1107 86-87 1.0023678207210551 1107 88-89 1.0193606433602795 1107 90-91 0.9936589565351426 1107 92-93 1.065933597840349 1107 94-95 1.0816131045880795 1107 96-97 1.042358982748354 1107 98-99 1.2472740977179448 1107 100-101 1.3313453510795874 1108 1 0.0797219186383451 1108 2 0.06165914597798405 1108 3 0.1666068101194682 1108 4 0.04848279921031917 1108 5 -0.058752142200845014 1108 6 0.0940716665135568 1108 7 0.10873453222360041 1108 8 0.09617062386163155 1108 9 0.19094128564699986 1108 10-11 0.14263350533870067 1108 12-13 0.2321201792938723 1108 14-15 0.34378697023864646 1108 16-17 0.33195061709010787 1108 18-19 0.2640727015860449 1108 20-21 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2316 64-65 -1.0859426435853088 2316 66-67 -1.2065081009831538 2316 68-69 -1.2952059615063831 2316 70-71 -1.1441667866837477 2316 72-73 -1.2581484365491704 2316 74-75 -1.120620675257495 2316 76-77 -1.212663898800841 2316 78-79 -1.4836494094851815 2316 80-81 -1.4768339688846375 2316 82-83 -1.316551184556534 2316 84-85 -1.6115839406530768 2316 86-87 -1.6481317977521144 2316 88-89 -1.7556779635217374 2316 90-91 -1.6933282532319467 2316 92-93 -1.83371348983815 2316 94-95 -1.7562184603986566 2316 96-97 -2.1215781016599173 2316 98-99 -2.0905056886270295 2316 100-101 -2.3100875547014503 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 18.0 4 41.0 5 120.0 6 257.0 7 624.0 8 1425.0 9 2333.0 10 3840.0 11 5938.0 12 8320.0 13 11614.0 14 15129.0 15 20453.0 16 25540.0 17 32679.0 18 31142.0 19 24951.0 20 24488.0 21 26229.0 22 28466.0 23 32175.0 24 37027.0 25 43282.0 26 51749.0 27 61609.0 28 73497.0 29 87464.0 30 102918.0 31 122822.0 32 147938.0 33 180955.0 34 228876.0 35 300725.0 36 390558.0 37 437474.0 38 205032.0 39 7398.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.62957352228962 19.215748310693943 15.157666163141995 22.997012003874453 2 16.01164540988778 20.78496552345757 38.964063274595524 24.239325792059127 3 19.243396575202524 24.57056703636933 29.76808511941676 26.417951269011386 4 13.342832062752152 17.165566168956307 35.74657274599763 33.74502902229391 5 14.157987841653819 37.29449939922066 33.28815020889384 15.259362550231678 6 32.555659014843364 35.64140834676222 16.98666576339982 14.816266874994593 7 29.225512618765435 32.65668821413759 19.803878256489014 18.313920910607965 8 26.899913625138865 34.79245709994512 19.29362876394583 19.014000510970185 9 27.039187233096456 16.41349379286537 19.649650302528222 36.897668671509955 10-11 25.545157980954293 26.596772816097943 26.469678892355013 21.388390310592758 12-13 26.3617373418668 23.869208208818886 27.20784703181456 22.561207417499755 14-15 23.01236021740376 25.015810167009416 25.823669162300778 26.148160453286046 16-17 22.615655650778905 28.549810404116876 26.24399438869697 22.59053955640725 18-19 22.516110154793697 26.499515515224065 29.165082647085 21.819291682897237 20-21 23.25514312390162 25.66646835642368 29.1092110483429 21.969177471331797 22-23 22.936453398474224 25.587949031264674 29.026259471235942 22.449338099025155 24-25 22.795989970240818 25.870774956737836 29.08821157343108 22.24502349959026 26-27 22.367738988721836 26.32627671008026 28.986550704896572 22.31943359630133 28-29 22.248289257002423 26.35897315782077 29.228192756323175 22.16454482885363 30-31 22.24436177197437 26.027450078808712 29.08980084855395 22.638387300662973 32-33 22.137888985702148 26.16464187622653 28.890775906615325 22.806693231455995 34-35 22.90296214154404 26.11341139108896 28.666223629018916 22.31740283834808 36-37 22.818853387848783 25.84950319048017 28.669304495506225 22.66233892616482 38-39 22.39061343181309 26.223136581024807 28.843834015048365 22.542415972113737 40-41 22.65724279250378 25.900212948854257 28.89484787733108 22.547696381310878 42-43 22.639346985606483 25.92479682487174 28.55330804387206 22.88254814564971 44-45 22.542557801580415 26.271283405452984 28.400925798293812 22.785232994672786 46-47 22.584990232830158 26.539279661765125 27.905998782752274 22.969731322652446 48-49 22.738245253436524 26.493137396567274 27.9280879733546 22.840529376641598 50-51 22.767502264784884 26.64630222067833 27.62835762917201 22.957837885364768 52-53 23.1381929312428 26.57444940079918 27.420829350012056 22.86652831794597 54-55 22.542269051703016 26.552766046384246 27.712948815889995 23.192016086022747 56-57 22.66808594821184 26.977027602119264 27.514627332292413 22.840259117376483 58-59 22.802830579878894 26.415844096443863 27.556993019784397 23.22433230389285 60-61 22.709976134305162 26.513496927872847 27.907728442049027 22.868798495772964 62-63 22.590896150089915 26.71438661991373 27.722343749436913 22.972373480559448 64-65 22.327695694356546 26.432541507429313 27.67538573761913 23.564377060595007 66-67 22.374197124160773 26.73979480325116 27.493935468665793 23.392072603922273 68-69 22.46186254749443 26.53161144533534 27.608983779137503 23.397542228032727 70-71 22.372988909997147 26.755568784250872 27.41921767481817 23.452224630933816 72-73 22.58587133992118 26.60789063932938 27.33467237887061 23.47156564187883 74-75 22.38575725410124 26.82070236964604 27.123463442501528 23.67007693375119 76-77 22.40541013128744 26.580758088968288 27.496579460176623 23.51725231956765 78-79 22.455597322727673 26.706506985783324 27.350755152354257 23.487140539134753 80-81 22.204998999767515 27.018027613625513 27.27053675538999 23.506436631216985 82-83 22.464551246812 26.815370889395563 27.091156511630004 23.628921352162433 84-85 22.31171591282393 26.495490386561205 27.198417405084218 23.994376295530643 86-87 22.404161363845628 26.46388042349532 27.266155277411343 23.86580293524771 88-89 22.1834631893178 26.541582226915622 27.12495135585084 24.150003227915736 90-91 22.100303790871063 26.535340536104297 27.33351919656623 24.030836476458415 92-93 22.26771539452186 26.231424422282366 27.265320289834698 24.23553989336107 94-95 22.0205051551241 26.125212682998388 27.29954903364739 24.55473312823012 96-97 22.148048507530614 26.09886975456585 27.269525513929395 24.483556223974137 98-99 21.928595082680317 26.102802328161783 27.001182035867043 24.967420553290854 100-101 21.9535383411668 25.88572729079613 27.42115070621945 24.739583661817623 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1265.0 1 869.5 2 672.5 3 900.0 4 798.5 5 763.0 6 1325.0 7 2829.0 8 4418.5 9 4825.5 10 4030.5 11 3258.0 12 3166.5 13 3366.0 14 3658.5 15 4010.0 16 4232.0 17 4282.5 18 4554.0 19 4833.0 20 5150.5 21 5867.0 22 6759.5 23 7875.5 24 9255.0 25 11113.0 26 13415.5 27 16073.5 28 18736.0 29 21896.0 30 26160.0 31 30716.5 32 35958.0 33 40171.5 34 43343.0 35 47528.5 36 52781.0 37 58376.5 38 63182.5 39 66006.5 40 68714.5 41 76273.5 42 85023.5 43 91886.5 44 96463.0 45 99359.0 46 100476.5 47 103781.5 48 112848.5 49 117040.0 50 116388.5 51 114029.0 52 108702.5 53 106739.5 54 105750.5 55 97014.0 56 85212.0 57 76118.0 58 68374.5 59 60096.5 60 50608.0 61 41601.0 62 34214.5 63 26567.5 64 20458.5 65 16567.0 66 12682.0 67 10191.0 68 7787.0 69 5297.0 70 4190.5 71 3584.5 72 3087.5 73 2684.5 74 2279.5 75 2003.0 76 1395.0 77 736.5 78 416.0 79 271.5 80 169.5 81 111.5 82 61.0 83 28.0 84 16.5 85 10.5 86 5.0 87 3.5 88 2.5 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.2431111814185587E-4 2 0.0536194381994535 3 0.0 4 1.8017284341214214E-4 5 0.04381803551783296 6 3.6034568682428426E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0061619112446952605 26-27 0.00218009140528692 28-29 1.0810370604728529E-4 30-31 0.0014053481786147088 32-33 3.963802555067127E-4 34-35 0.01189140766520138 36-37 0.021945052327598913 38-39 0.004558372938327196 40-41 0.0012071580508613523 42-43 0.0015855210220268507 44-45 0.0075852767076511835 46-47 0.0 48-49 0.0 50-51 5.405185302364264E-4 52-53 0.0 54-55 0.0011891407665201381 56-57 0.0 58-59 0.022647726416906266 60-61 0.0 62-63 0.008756400189830107 64-65 0.015458829964761797 66-67 0.005999755685624333 68-69 0.016053400348021864 70-71 3.6034568682428426E-5 72-73 7.206913736485685E-5 74-75 0.02335040050621362 76-77 0.051457364078507796 78-79 0.031404126606736374 80-81 0.027314203061280747 82-83 0.037403882292360704 84-85 0.01547684724910301 86-87 0.009134763160995606 88-89 0.08741986362357136 90-91 0.02419721287025069 92-93 0.02536833635242961 94-95 0.026575494403290968 96-97 0.038538971205857205 98-99 0.054358146857443286 100-101 0.013458911402887018 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2775113.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.56895073039232 #Duplication Level Percentage of deduplicated Percentage of total 1 69.81043965247909 29.717571660334087 2 15.139254856612776 12.889243881720036 3 5.924496719421768 7.565988268543085 4 2.8347157860070844 4.826835065168035 5 1.6319112207549293 3.4734374176345493 6 1.0137511449565757 2.589259352552116 7 0.6937775552171145 2.067336779612257 8 0.47609133484509175 1.6213366860949936 9 0.35511739436366485 1.3605277377754932 >10 1.8337457402152202 14.334078869676805 >50 0.16049048172981917 4.725899613946649 >100 0.11475672521161909 9.534413266546588 >500 0.008482509766988935 2.484959762317672 >1k 0.0027144031254312476 1.7029799225530657 >5k 8.482509766972649E-5 0.2758017185119443 >10k+ 1.6965019533945298E-4 0.8303299970125895 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12774 0.4603055803493407 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10221 0.36830932650310094 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7638 0.2752320355963883 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2925 0.10540111339610315 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 7.206913736485685E-5 0.0 0.0 0.0 0.0 14-15 1.8017284341214214E-4 0.0 0.0 1.8017284341214213E-5 0.0 16-17 1.8017284341214214E-4 0.0 0.0 5.4051853023642636E-5 0.0 18-19 2.702592651182132E-4 0.0 0.0 7.206913736485685E-5 3.6034568682428426E-5 20-21 2.882765494594274E-4 0.0 0.0 1.441382747297137E-4 3.6034568682428426E-5 22-23 2.882765494594274E-4 0.0 0.0 2.702592651182132E-4 3.6034568682428426E-5 24-25 6.486222362837117E-4 0.0 0.0 6.306049519424975E-4 3.6034568682428426E-5 26-27 6.486222362837117E-4 0.0 0.0 0.0015855210220268507 3.6034568682428426E-5 28-29 8.287950796958539E-4 0.0 0.0 0.004324148241891411 3.6034568682428426E-5 30-31 8.648296483782823E-4 0.0 0.0 0.01435977561994773 3.6034568682428426E-5 32-33 8.648296483782823E-4 0.0 0.0 0.03417878839528336 3.6034568682428426E-5 34-35 9.368987857431391E-4 0.0 0.0 0.062321786536259964 3.6034568682428426E-5 36-37 0.0010450024917904243 0.0 0.0 0.1108963851201735 3.6034568682428426E-5 38-39 0.0011531061978377096 0.0 0.0 0.1849654410469051 3.6034568682428426E-5 40-41 0.0011531061978377096 0.0 0.0 0.26919624534208153 3.6034568682428426E-5 42-43 0.0013152617569086376 0.0 0.0 0.35351713605896407 3.6034568682428426E-5 44-45 0.0014774173159795656 0.0 0.0 0.4561796222352027 3.6034568682428426E-5 46-47 0.0017296592967565643 0.0 0.0 0.5837419953709992 3.6034568682428426E-5 48-49 0.0018017284341214214 0.0 0.0 0.7157186031703935 3.6034568682428426E-5 50-51 0.0018017284341214214 0.0 0.0 0.8624153322765595 3.6034568682428426E-5 52-53 0.0018017284341214214 0.0 0.0 1.0072022292425569 3.6034568682428426E-5 54-55 0.0018197457184626355 0.0 0.0 1.1671416623395154 3.6034568682428426E-5 56-57 0.0019098321401687067 0.0 0.0 1.3489360613423669 3.6034568682428426E-5 58-59 0.0019098321401687067 0.0 0.0 1.5488017965394563 3.6034568682428426E-5 60-61 0.0019098321401687067 0.0 0.0 1.7687748210613408 3.6034568682428426E-5 62-63 0.0019098321401687067 0.0 0.0 1.9846759393221105 3.6034568682428426E-5 64-65 0.0019278494245099208 0.0 0.0 2.2111892380598555 3.6034568682428426E-5 66-67 0.001945866708851135 0.0 0.0 2.4785116858304512 3.6034568682428426E-5 68-69 0.0019819012775335633 0.0 0.0 2.7660134920632062 3.6034568682428426E-5 70-71 0.002035953130557206 0.0 0.0 3.0809736396319716 3.6034568682428426E-5 72-73 0.002090004983580849 0.0 0.0 3.4014110416404666 3.6034568682428426E-5 74-75 0.0021260395522632774 0.0 0.0 3.7337939031671867 3.6034568682428426E-5 76-77 0.00218009140528692 0.0 0.0 4.086950693539326 3.6034568682428426E-5 78-79 0.002198108689628134 0.0 0.0 4.476466363712037 3.6034568682428426E-5 80-81 0.0026665580824997035 0.0 0.0 4.90061125438856 3.6034568682428426E-5 82-83 0.002702592651182132 0.0 0.0 5.328864085894881 3.6034568682428426E-5 84-85 0.002702592651182132 0.0 0.0 5.760648305132079 3.6034568682428426E-5 86-87 0.002720609935523346 0.0 0.0 6.212521796409732 3.6034568682428426E-5 88-89 0.0027386272198645606 0.0 0.0 6.695366999469932 3.6034568682428426E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6805 0.0 50.491955 1 GTATCAA 12105 0.0 41.37975 1 ATCAACG 15895 0.0 31.169668 3 TATCAAC 16275 0.0 30.602177 2 TCAACGC 16345 0.0 30.311527 4 CAACGCA 16500 0.0 29.997993 5 AACGCAG 17015 0.0 29.16222 6 GTACATG 14110 0.0 26.035416 1 ACGCAGA 19455 0.0 25.431545 7 TACATGG 14295 0.0 25.266289 2 CGCAGAG 19615 0.0 25.12727 8 CGTATAG 175 1.4805119E-7 24.440859 1 ACATGGG 14795 0.0 23.566198 3 GCAGAGT 21075 0.0 23.071117 9 GAGTACT 10950 0.0 22.765722 12-13 AGTACTT 11225 0.0 22.73675 12-13 GTAAGGT 950 0.0 21.500732 4 AGAGTAC 18580 0.0 21.44137 10-11 GTACTTT 11640 0.0 21.273432 14-15 CATGGGA 8325 0.0 21.054773 4 >>END_MODULE