FastQCFastQC Report
Thu 26 May 2016
SRR938326_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938326_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752501
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135670.4928971869583335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102810.3735148506758036No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74340.27008164574690435No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG27630.1003814349204596No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67350.047.6443821
GTATCAA119000.040.6877251
ATCAACG157250.030.6759533
TATCAAC162050.029.7081222
TCAACGC161900.029.7068314
CAACGCA163000.029.562525
AACGCAG168250.028.6636146
GTACATG138900.026.5637171
TACATGG140300.026.0146682
ACGCAGA189750.025.2874097
CGCAGAG193100.024.7749028
ACATGGG144400.024.3220333
GCAGAGT203200.023.0048989
TGGTATC35300.022.6177482
AGTACTT110400.022.61147512-13
GAGTACT106000.022.13840512-13
AGAGTAC182300.021.60254910-11
TAGCGCG2901.05501385E-1021.3043374
GTGGTAT39100.020.8213421
CATGGGA83050.020.7725434
Produced by FastQC (version 0.11.5)