##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938326_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2752501 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.255315075271543 31.0 31.0 34.0 28.0 34.0 2 31.355802595530392 31.0 31.0 34.0 28.0 34.0 3 31.42034244492554 31.0 31.0 34.0 28.0 34.0 4 35.06022958756418 37.0 35.0 37.0 32.0 37.0 5 35.088278623695324 37.0 35.0 37.0 32.0 37.0 6 35.12559014510803 37.0 35.0 37.0 32.0 37.0 7 35.05212096199057 37.0 35.0 37.0 32.0 37.0 8 35.020577649199765 37.0 35.0 37.0 32.0 37.0 9 36.54944866505044 39.0 37.0 39.0 32.0 39.0 10-11 36.4035984001459 39.0 37.0 39.0 32.0 39.0 12-13 36.26186348342834 39.0 35.0 39.0 32.0 39.0 14-15 37.28729762495999 39.5 36.5 41.0 31.5 41.0 16-17 37.165610657362166 39.0 36.0 41.0 31.0 41.0 18-19 37.17153981052141 39.5 36.5 41.0 31.0 41.0 20-21 37.26430399117021 40.0 37.0 41.0 31.0 41.0 22-23 37.18804607155456 39.5 36.5 41.0 31.0 41.0 24-25 37.08107680978136 39.0 36.0 41.0 31.0 41.0 26-27 36.91397169337995 39.0 36.0 41.0 30.5 41.0 28-29 36.73592979621079 39.0 36.0 41.0 30.0 41.0 30-31 36.51358400959709 39.0 35.5 40.5 30.0 41.0 32-33 36.24242879475793 39.0 35.0 40.0 30.0 41.0 34-35 35.9969930982768 38.0 35.0 40.0 29.0 41.0 36-37 35.7274593179076 38.0 35.0 40.0 27.5 41.0 38-39 35.493059221413546 38.0 34.5 40.0 27.0 41.0 40-41 35.423090309503976 38.0 35.0 40.0 27.0 41.0 42-43 35.24141716933073 38.0 34.0 40.0 26.0 41.0 44-45 34.93532154938363 38.0 34.0 40.0 24.5 41.0 46-47 34.5408780959571 38.0 33.5 40.0 24.0 41.0 48-49 34.173083497517354 38.0 33.0 40.0 23.0 41.0 50-51 33.41521964932983 37.5 32.5 39.5 21.5 40.5 52-53 33.47657130733104 37.5 33.0 39.5 22.0 40.5 54-55 33.61939741347959 38.0 33.0 40.0 20.5 41.0 56-57 33.34657607753821 37.5 32.5 40.0 19.5 41.0 58-59 33.04776456030352 37.0 32.0 40.0 18.0 41.0 60-61 32.631703131079696 36.5 31.5 39.5 15.5 41.0 62-63 32.13599849736658 36.0 31.0 39.0 11.5 41.0 64-65 31.66789821329765 35.0 31.0 39.0 8.0 40.0 66-67 31.161946716822264 35.0 30.0 38.5 7.0 40.0 68-69 30.643482781659298 35.0 29.5 38.0 2.0 40.0 70-71 30.05462323174451 34.5 29.0 37.0 2.0 39.5 72-73 29.525519336777716 34.0 28.0 36.5 2.0 39.0 74-75 28.949013097542924 34.0 27.5 36.0 2.0 39.0 76-77 28.26180044984543 33.5 26.0 35.5 2.0 38.0 78-79 27.53279199535259 33.0 25.5 35.0 2.0 37.0 80-81 26.91470575305876 32.5 25.0 35.0 2.0 37.0 82-83 26.30011051767102 32.0 23.5 35.0 2.0 36.0 84-85 25.679821006422884 32.0 21.0 35.0 2.0 36.0 86-87 25.06062886080695 31.0 19.0 34.0 2.0 35.0 88-89 24.335802057837583 31.0 15.0 34.0 2.0 35.0 90-91 23.56730824075995 30.5 6.0 34.0 2.0 35.0 92-93 22.77115285335046 30.0 2.0 34.0 2.0 35.0 94-95 21.866464716997378 29.5 2.0 34.0 2.0 35.0 96-97 20.687283674011383 29.0 2.0 34.0 2.0 35.0 98-99 19.264877832923585 26.5 2.0 34.0 2.0 35.0 100-101 15.746990645961617 13.0 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5025466783289971 1101 2 0.5546341191790773 1101 3 0.583012074678777 1101 4 0.43510384513865574 1101 5 0.47497680924616503 1101 6 0.5054265207395829 1101 7 0.364159493876798 1101 8 0.523475744364994 1101 9 0.365003405032887 1101 10-11 0.5959556725024768 1101 12-13 0.5852533457369518 1101 14-15 0.6390046252418173 1101 16-17 0.7410382177107664 1101 18-19 0.6284672835739897 1101 20-21 0.7571160519543625 1101 22-23 0.7159057309913734 1101 24-25 0.7520128018500785 1101 26-27 0.7454554512923153 1101 28-29 0.7963151339175809 1101 30-31 0.6024336133986807 1101 32-33 0.7423413568645216 1101 34-35 0.7780234217753375 1101 36-37 0.8430054141760976 1101 38-39 0.77403472284918 1101 40-41 0.8586305627299211 1101 42-43 0.9342848631781209 1101 44-45 0.826025434537975 1101 46-47 0.9383327443805527 1101 48-49 0.858773196265517 1101 50-51 0.7299284206725574 1101 52-53 0.7511739642014632 1101 54-55 0.9182521381776425 1101 56-57 0.8451967298450782 1101 58-59 0.8055531478951465 1101 60-61 0.7731566356302508 1101 62-63 0.784367646859188 1101 64-65 0.7410495582280241 1101 66-67 0.7445509734780877 1101 68-69 0.6938050953585737 1101 70-71 0.9463800634222714 1101 72-73 1.1259972258772883 1101 74-75 0.7907094461018609 1101 76-77 0.8794974854280468 1101 78-79 1.089154653625254 1101 80-81 1.158001810820064 1101 82-83 0.9295499110754086 1101 84-85 1.1534222257736708 1101 86-87 0.96624988301107 1101 88-89 0.9287372308366102 1101 90-91 0.8947079364076629 1101 92-93 0.9815868954288085 1101 94-95 0.92377884018617 1101 96-97 1.2238060406326419 1101 98-99 1.4091445490733392 1101 100-101 1.0733605846151004 1102 1 0.5213617906094541 1102 2 0.46773727564857026 1102 3 0.4542978186377873 1102 4 0.3653793342260272 1102 5 0.4457361928768364 1102 6 0.40697344466274643 1102 7 0.29728926839270997 1102 8 0.4031787915927936 1102 9 0.3684160539900887 1102 10-11 0.4779666428302747 1102 12-13 0.5395255421873628 1102 14-15 0.516167956959606 1102 16-17 0.5291291806532072 1102 18-19 0.6450498649090477 1102 20-21 0.4697732407501576 1102 22-23 0.49529313126986096 1102 24-25 0.3848687797555641 1102 26-27 0.46012461051593334 1102 28-29 0.4255673668331852 1102 30-31 0.5016266865521501 1102 32-33 0.5188139271984724 1102 34-35 0.46698685658035544 1102 36-37 0.3596118440498586 1102 38-39 0.548375772338737 1102 40-41 0.5305472159337015 1102 42-43 0.4466644088249936 1102 44-45 0.5492050601928469 1102 46-47 0.5434957222167967 1102 48-49 0.35136576321451685 1102 50-51 0.32464319984278234 1102 52-53 0.42306730738193465 1102 54-55 0.24378753787146223 1102 56-57 0.3504154055587989 1102 58-59 0.36618221080144053 1102 60-61 0.2983383845743717 1102 62-63 0.13433972098967928 1102 64-65 0.10971488629929027 1102 66-67 0.15199271765250444 1102 68-69 1.528839835920337E-4 1102 70-71 0.08928235365129211 1102 72-73 0.10330572595806231 1102 74-75 -0.025021511609296 1102 76-77 0.1770888599501106 1102 78-79 0.18452380592509954 1102 80-81 0.22165294516426925 1102 82-83 0.2712995475775202 1102 84-85 0.21173159517214657 1102 86-87 0.28575244865224647 1102 88-89 0.40038770357619313 1102 90-91 0.3514713050522218 1102 92-93 0.4794948400694281 1102 94-95 0.62539361704847 1102 96-97 0.8655592159501708 1102 98-99 1.1504324470939125 1102 100-101 1.0030896344431586 1103 1 0.4332614525091181 1103 2 0.5363443442556388 1103 3 0.4276514169913881 1103 4 0.4908780847247769 1103 5 0.49699461913526477 1103 6 0.5211453088346047 1103 7 0.3464844425878866 1103 8 0.32205571046971215 1103 9 0.34125515182918775 1103 10-11 0.4476531125167469 1103 12-13 0.4013939665557871 1103 14-15 0.5092799500716012 1103 16-17 0.5754893520133848 1103 18-19 0.6196345894937707 1103 20-21 0.5992344327113486 1103 22-23 0.6322920182687497 1103 24-25 0.6190150764018654 1103 26-27 0.6454379833293018 1103 28-29 0.6010750423408595 1103 30-31 0.6300405024659668 1103 32-33 0.580861114245657 1103 34-35 0.6160410681345709 1103 36-37 0.6665219009599141 1103 38-39 0.4546736786366523 1103 40-41 0.6435050163915008 1103 42-43 0.6486872358478237 1103 44-45 0.6610866095743972 1103 46-47 0.5074465611676402 1103 48-49 0.47819407754283105 1103 50-51 0.5182712684708477 1103 52-53 0.5827225920372214 1103 54-55 0.6333576149415379 1103 56-57 0.6199191750625701 1103 58-59 0.616779398898629 1103 60-61 0.6249766487126394 1103 62-63 0.6602839344338918 1103 64-65 0.4994096509940569 1103 66-67 0.6145404927002822 1103 68-69 0.6453391541698643 1103 70-71 0.7104944748634132 1103 72-73 0.7290630392153759 1103 74-75 0.45793828385049906 1103 76-77 0.5847858926471439 1103 78-79 0.5349760938773862 1103 80-81 0.5519045254158499 1103 82-83 0.5229244242023974 1103 84-85 0.4682455391860927 1103 86-87 0.4107406361404351 1103 88-89 0.5796491328376234 1103 90-91 0.58433791291883 1103 92-93 0.7806154536900429 1103 94-95 0.7839779006327525 1103 96-97 0.7877370756280335 1103 98-99 0.8027902244516909 1103 100-101 0.680856124709651 1104 1 0.4987308244094173 1104 2 0.45167209413817133 1104 3 0.46381358685637863 1104 4 0.4915791797369735 1104 5 0.4828504364769728 1104 6 0.46555756645683033 1104 7 0.48516301672816553 1104 8 0.37289768472685836 1104 9 0.46934728757064903 1104 10-11 0.5113648638430064 1104 12-13 0.4377186523628893 1104 14-15 0.5458626118832797 1104 16-17 0.5271668906733353 1104 18-19 0.5245153772071021 1104 20-21 0.5294699769767632 1104 22-23 0.5923253385072726 1104 24-25 0.6265474117786454 1104 26-27 0.5883852591137 1104 28-29 0.5656395518852122 1104 30-31 0.5597865044224832 1104 32-33 0.5663304146196921 1104 34-35 0.6245841247492621 1104 36-37 0.4638799955880657 1104 38-39 0.5918635434740054 1104 40-41 0.4534230555372787 1104 42-43 0.4274643181701592 1104 44-45 0.5365961654842835 1104 46-47 0.4550814899769904 1104 48-49 0.5560599839402514 1104 50-51 0.3557714197764241 1104 52-53 0.4506714771627216 1104 54-55 0.588261607427981 1104 56-57 0.566402118247602 1104 58-59 0.5596050800212993 1104 60-61 0.6547895552432053 1104 62-63 0.7260517221923308 1104 64-65 0.6917056938305954 1104 66-67 0.7389200710694119 1104 68-69 0.7152003766968953 1104 70-71 0.7286126296616722 1104 72-73 0.7740650997222005 1104 74-75 0.7806918499853417 1104 76-77 0.8824703946647361 1104 78-79 0.9798446753573664 1104 80-81 0.974055056305847 1104 82-83 1.057580415848129 1104 84-85 1.004074080259226 1104 86-87 0.8952252811005295 1104 88-89 1.0218735118520357 1104 90-91 1.0142711899114687 1104 92-93 1.036989338567956 1104 94-95 1.1246307099095993 1104 96-97 1.1357871456687576 1104 98-99 0.8413003817324594 1104 100-101 0.7567573969969583 1105 1 0.4247590917668731 1105 2 0.3785000838102235 1105 3 0.36063497858766524 1105 4 0.39017767333072584 1105 5 0.24765966279163365 1105 6 0.30354476030920097 1105 7 0.3526716268820067 1105 8 0.31172203864760206 1105 9 0.3844276595970726 1105 10-11 0.28074234336609294 1105 12-13 0.4240125027220145 1105 14-15 0.5035120795999717 1105 16-17 0.4652309722463315 1105 18-19 0.5034754390138119 1105 20-21 0.5435915427115248 1105 22-23 0.5188489147606177 1105 24-25 0.4939027892592236 1105 26-27 0.5531276455348575 1105 28-29 0.5139993467030237 1105 30-31 0.5684230685580687 1105 32-33 0.6213788718876927 1105 34-35 0.604083843568965 1105 36-37 0.463445450231724 1105 38-39 0.508801505885863 1105 40-41 0.40167919490527737 1105 42-43 0.5151078472106292 1105 44-45 0.5801346315848406 1105 46-47 0.5793949546478174 1105 48-49 0.4273247472298607 1105 50-51 0.2986761314262907 1105 52-53 0.35386132470051734 1105 54-55 0.375696051644141 1105 56-57 0.41783215306948307 1105 58-59 0.432731008022202 1105 60-61 0.6109141758351413 1105 62-63 0.4586829476089136 1105 64-65 0.3350098827286807 1105 66-67 0.6154404171912446 1105 68-69 0.5106000075087493 1105 70-71 0.5329695872590428 1105 72-73 0.4261356537374148 1105 74-75 0.3407674641869036 1105 76-77 0.38973878629223435 1105 78-79 0.5265491336801986 1105 80-81 0.49304544957700713 1105 82-83 0.5091678931048236 1105 84-85 0.5174658216072423 1105 86-87 0.7011141020919389 1105 88-89 0.6089224451672948 1105 90-91 0.6037239897471025 1105 92-93 0.8915009056159562 1105 94-95 0.8894426500642645 1105 96-97 0.6415397583685767 1105 98-99 0.8211250332489257 1105 100-101 0.5767431605894604 1106 1 0.3412561819324189 1106 2 0.26128249400581893 1106 3 0.13127622869890487 1106 4 0.33813256261835534 1106 5 0.30701234362667407 1106 6 0.05614674421197918 1106 7 0.20266083917781685 1106 8 0.182362848972339 1106 9 0.16725798065156994 1106 10-11 0.21979582450908453 1106 12-13 0.135524781043749 1106 14-15 0.4530575085108097 1106 16-17 0.17484770607098454 1106 18-19 0.008153344553882391 1106 20-21 0.26557914745831823 1106 22-23 0.28399044064386203 1106 24-25 0.37441286140490604 1106 26-27 0.2070844176261062 1106 28-29 0.200407188665487 1106 30-31 0.13709501938890867 1106 32-33 0.31215967919084164 1106 34-35 0.08641483879029721 1106 36-37 0.06497540279686831 1106 38-39 0.24190801286346897 1106 40-41 0.42908080431691076 1106 42-43 0.018173935221739157 1106 44-45 0.13338967257294598 1106 46-47 0.01022740323418958 1106 48-49 0.3876655119202823 1106 50-51 -0.08197169737361776 1106 52-53 0.19096899633625242 1106 54-55 -0.037206948010243934 1106 56-57 -0.4017872789747017 1106 58-59 -0.7259989998008223 1106 60-61 0.05891239702808804 1106 62-63 0.022893523077314626 1106 64-65 0.31224168281105236 1106 66-67 -0.21623032914196827 1106 68-69 -0.4196088496879149 1106 70-71 -0.18579386503395057 1106 72-73 0.22955917176038199 1106 74-75 0.3303031970988677 1106 76-77 0.26134900248093373 1106 78-79 0.39254573387184166 1106 80-81 0.2783982049170497 1106 82-83 0.4357522627821169 1106 84-85 0.5209603780851424 1106 86-87 0.751884478694052 1106 88-89 0.3741772807492296 1106 90-91 0.42134879598234676 1106 92-93 0.39782671704791994 1106 94-95 0.11515615192378803 1106 96-97 0.19936722081081015 1106 98-99 0.1808158064246399 1106 100-101 -0.1486276958455992 1107 1 0.33205486827085906 1107 2 0.2649488624529184 1107 3 0.22825915142265174 1107 4 0.291771695007526 1107 5 0.2405042704503444 1107 6 0.27356663026045425 1107 7 0.14385974166001603 1107 8 0.19690793826311648 1107 9 0.26088758447067306 1107 10-11 0.388003313047939 1107 12-13 0.28289378781805397 1107 14-15 0.33707894642263625 1107 16-17 0.4128919739182706 1107 18-19 0.3337485468203951 1107 20-21 0.3871167067361583 1107 22-23 0.4189774158138775 1107 24-25 0.4156262324587203 1107 26-27 0.33701393536905755 1107 28-29 0.38618028332845 1107 30-31 0.31512101022747174 1107 32-33 0.3965523275613094 1107 34-35 0.3573146032429477 1107 36-37 0.3105764149239221 1107 38-39 0.44673369897721926 1107 40-41 0.43942277978663924 1107 42-43 0.46441764603525115 1107 44-45 0.4916604178065782 1107 46-47 0.5626957278421401 1107 48-49 0.6051435339606144 1107 50-51 0.5909742795222783 1107 52-53 0.5216215937190256 1107 54-55 0.5896188056484348 1107 56-57 0.6154904511768322 1107 58-59 0.6848026301005916 1107 60-61 0.6399040587192317 1107 62-63 0.7398624034354313 1107 64-65 0.7643573780458546 1107 66-67 0.7588804252189476 1107 68-69 0.8231276718905072 1107 70-71 0.7941855449798219 1107 72-73 0.7639497576428163 1107 74-75 0.8809860052579488 1107 76-77 0.9200940950367951 1107 78-79 0.8749514024612957 1107 80-81 0.8326761602598829 1107 82-83 0.8246884663510556 1107 84-85 0.6702306124946951 1107 86-87 0.8023208475735863 1107 88-89 0.8875504905127372 1107 90-91 1.0029337189580758 1107 92-93 1.0179212201247658 1107 94-95 1.0304641623905084 1107 96-97 0.8048362536434972 1107 98-99 0.7914308403547494 1107 100-101 0.5009563981470269 1108 1 0.42563518800527333 1108 2 0.39638974040832764 1108 3 0.3631266102038566 1108 4 0.37655072435202186 1108 5 0.34942149580560766 1108 6 0.43232643494008016 1108 7 0.40257233928788594 1108 8 0.3992456927491048 1108 9 0.3387235624681608 1108 10-11 0.4473680957255368 1108 12-13 0.5223348007875472 1108 14-15 0.6267755405726945 1108 16-17 0.46374314913925474 1108 18-19 0.5750594090863999 1108 20-21 0.5247688908283337 1108 22-23 0.6617050282525909 1108 24-25 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pass #Quality Count 2 8466.0 3 2683.0 4 2755.0 5 3149.0 6 3650.0 7 4536.0 8 5700.0 9 7329.0 10 9010.0 11 11513.0 12 14379.0 13 17737.0 14 20237.0 15 24767.0 16 29629.0 17 35807.0 18 32819.0 19 27816.0 20 28961.0 21 30945.0 22 34813.0 23 38748.0 24 43851.0 25 49443.0 26 56817.0 27 65130.0 28 75420.0 29 86777.0 30 101901.0 31 120546.0 32 145402.0 33 176938.0 34 220958.0 35 280775.0 36 347352.0 37 372312.0 38 200351.0 39 13079.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.99858191084602 18.450410011942378 14.531155193041753 23.019852884169854 2 15.591886791355524 20.10818677739782 40.18438714029823 24.115539290948426 3 19.166055118233302 24.56752712349062 29.346219285820474 26.920198472455603 4 12.91743864940915 16.472503237874644 36.22392025104752 34.38613786166869 5 14.149923133213104 37.215266627236616 33.1650467507833 15.46976348876698 6 32.38832221020923 35.399163711689205 17.329352323713813 14.88316175438775 7 29.29438928469277 32.638852292728735 19.91159013818993 18.155168284388566 8 26.921062664249106 34.470007126925786 19.49986498455227 19.109065224272832 9 27.36246386366726 16.62314920552769 19.6935290068351 36.32085792396996 10-11 25.536296148219495 26.52834543356383 26.50875487265227 21.42660354556441 12-13 26.5589169297867 23.88213433886832 27.17392272221339 22.38502600913159 14-15 23.2017995678435 24.943163842772954 25.939202620196365 25.91583396918718 16-17 22.661731866873065 28.485167526192352 26.274029301706324 22.579071305228258 18-19 22.600204769151315 26.414857066843645 29.230342034265576 21.75459612973947 20-21 23.35163137550825 25.645138660193652 29.117023140444196 21.8862068238539 22-23 22.99858971143606 25.56660523914932 29.014288258620752 22.42051679079387 24-25 22.800112919359908 25.750921923315538 29.162279044242684 22.28668611308187 26-27 22.35645296897531 26.257393949329476 29.006327363164907 22.379825718530302 28-29 22.245722399341833 26.336709883528737 29.304547211579195 22.113020505550235 30-31 22.203871559659706 26.008020251279884 29.118575692938954 22.669532496121462 32-33 22.138483381107363 26.109065095054934 28.97103607005953 22.78141545377817 34-35 22.907416369987757 26.134248199071624 28.648105583464083 22.31022984747654 36-37 22.813686001683973 25.806669696821555 28.670275107410877 22.7093691940836 38-39 22.410468599869464 26.17224464817579 28.7897216775227 22.627565074432038 40-41 22.6495161214713 25.95695514289234 28.866385859285487 22.527142876350876 42-43 22.56348872381542 25.8917274063867 28.626777222768514 22.91800664702937 44-45 22.44124376559453 26.30079377118063 28.43991581792766 22.818046645297187 46-47 22.59515917938447 26.52577340564455 27.925737361464265 22.95333005350671 48-49 22.762299994567993 26.472922725820176 27.89975970189737 22.865017577714465 50-51 22.734199291488782 26.664910527750024 27.673049323280953 22.927840857480245 52-53 23.060785143710476 26.57819917267239 27.566523207502264 22.794492476114865 54-55 22.45398264767165 26.584993689583463 27.796008677435886 23.165014985309 56-57 22.613813367768227 27.003680992294658 27.588239718310163 22.79426592162695 58-59 22.778319771381646 26.323720970792298 27.695903436723913 23.202055821102142 60-61 22.721604534554174 26.43839473875445 28.016114381222295 22.82388634546908 62-63 22.54101852411972 26.609290962073068 27.926185458463383 22.923505055343828 64-65 22.35054916766931 26.32751172064543 27.914547152628444 23.40739195905681 66-67 22.39944033961837 26.673554030533897 27.649017660511333 23.277987969336394 68-69 22.51343579350253 26.45085950382895 27.819845279682326 23.215859422986192 70-71 22.413424390867863 26.62905973774718 27.595524015979585 23.36199185540537 72-73 22.644156940892437 26.521494088226383 27.501357211535314 23.332991759345862 74-75 22.478834107147982 26.731289910321802 27.38800581841755 23.40187016411266 76-77 22.63942874116492 26.541153472671997 27.457503949160074 23.36191383700302 78-79 22.70033382309556 26.641121279369383 27.40528478147449 23.253260116060567 80-81 22.514142665615427 26.987984638358327 27.269893289636705 23.227979406389544 82-83 22.707754621114145 26.80058183229213 27.097020552878742 23.394642993714985 84-85 22.662054126797734 26.473140063480255 27.18149786073874 23.68330794898327 86-87 22.797824202351652 26.4189336978314 27.290847799531882 23.49239430028507 88-89 22.669661181870463 26.42448888367998 27.275000249845778 23.630849684603778 90-91 22.631024609795507 26.532415339090665 27.262197502022527 23.574362549091298 92-93 22.869205242662847 26.11816123063327 27.28132799202292 23.73130553468097 94-95 22.653960316429185 26.048441976019777 27.327041939496137 23.9705557680549 96-97 22.899973378568568 25.981735699157838 27.199434499012824 23.91885642326077 98-99 22.738781277634253 26.017871610100123 26.902858073121305 24.340489039144316 100-101 23.18333379712005 25.518963693139067 27.194106744059027 24.103595765681856 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1350.0 1 928.0 2 726.5 3 950.5 4 839.0 5 775.5 6 1270.0 7 2811.5 8 4580.0 9 5108.5 10 4251.0 11 3438.0 12 3458.0 13 3670.5 14 3885.0 15 4079.5 16 4211.0 17 4401.5 18 4606.5 19 4802.0 20 5198.5 21 5870.5 22 6782.0 23 7895.0 24 9268.5 25 11183.5 26 13439.5 27 16100.5 28 18753.5 29 21740.0 30 25768.5 31 30088.5 32 35221.5 33 39908.5 34 43552.0 35 47382.0 36 52451.5 37 58002.0 38 62610.5 39 65646.5 40 68503.0 41 75610.5 42 84080.5 43 90714.0 44 95454.5 45 98325.5 46 98988.0 47 101849.5 48 110441.5 49 115110.0 50 114028.5 51 111318.5 52 107115.5 53 105295.5 54 103430.5 55 95055.0 56 83999.0 57 75381.5 58 68107.5 59 60201.5 60 50891.0 61 42170.5 62 34930.0 63 27136.0 64 21058.0 65 17090.0 66 13317.0 67 10634.5 68 8125.0 69 5722.0 70 4544.5 71 3806.5 72 3239.0 73 2835.5 74 2270.5 75 1862.5 76 1378.0 77 834.5 78 496.0 79 316.0 80 177.5 81 112.0 82 65.5 83 40.0 84 21.0 85 15.0 86 16.5 87 11.0 88 8.0 89 9.0 90 7.0 91 5.0 92 4.5 93 2.5 94 1.5 95 1.5 96 2.5 97 2.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18681192123090964 2 0.04842868358630933 3 0.046793806796073825 4 0.053333313957015814 5 0.059509515164572147 6 0.06448680672595578 7 0.0362579341478895 8 0.03502269390637823 9 0.0405086138025018 10-11 0.042724780118154365 12-13 0.03863758814256561 14-15 0.03456856146464615 16-17 0.032207072767639316 18-19 0.040326960825808965 20-21 0.05039053573459193 22-23 0.046902798582089525 24-25 0.06777472560409606 26-27 0.05209807371550455 28-29 0.044377822206059146 30-31 0.05322432217100012 32-33 0.0492279566837578 34-35 0.04906446900473425 36-37 0.04601269899629464 38-39 0.026485004001815075 40-41 0.02448682125819391 42-43 0.02161670422644715 44-45 0.004850134477698645 46-47 0.011734782294357024 48-49 0.010699360327207873 50-51 0.009118979429980226 52-53 0.009663938360058725 54-55 0.011098996875932108 56-57 0.009227971215995924 58-59 0.011480468126987057 60-61 0.010935509196908557 62-63 0.004232514356943013 64-65 0.007247953770044044 66-67 0.00426884495228158 68-69 0.008882830560279543 70-71 0.022325150835549198 72-73 0.01943686850613315 74-75 0.019981827436211647 76-77 0.012116253545411973 78-79 0.005267936324092162 80-81 0.006939143709666228 82-83 0.005867391147178512 84-85 0.0054132587054464285 86-87 0.00810172276050036 88-89 0.029191633354538293 90-91 0.0361489423618738 92-93 0.022415977323895612 94-95 0.031189816098159455 96-97 0.034822875632016116 98-99 0.021598538928777864 100-101 0.12325154468608732 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2752501.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.011696001569135 #Duplication Level Percentage of deduplicated Percentage of total 1 72.29828439624535 32.54268398678785 2 13.99397902029225 12.597854590274618 3 5.398747492662162 7.290203427868285 4 2.639251929038877 4.751888220058114 5 1.4855152675817873 3.3432780815040517 6 0.9123278977191271 2.4639255591530405 7 0.5859662800318464 1.8462735244774657 8 0.4395892479746412 1.5829326076314352 9 0.30438021569348284 1.2330602763918384 >10 1.6871806205018454 13.937628499225774 >50 0.14351654499418004 4.495470378076601 >100 0.10076788973606968 8.751831016796013 >500 0.0076462059509860335 2.3468277686034824 >1k 0.00260296372798938 1.6707925732015412 >5k 8.134261649966812E-5 0.2721861808160817 >10k+ 1.6268523299933625E-4 0.8731633091339678 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13567 0.4928971869583335 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10281 0.3735148506758036 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7434 0.27008164574690435 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2763 0.1003814349204596 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.633059533856663E-5 0.0 0.0 0.0 0.0 6 7.266119067713326E-5 0.0 0.0 0.0 0.0 7 1.089917860156999E-4 0.0 0.0 0.0 0.0 8 1.089917860156999E-4 0.0 0.0 0.0 0.0 9 1.089917860156999E-4 0.0 0.0 0.0 0.0 10-11 1.089917860156999E-4 0.0 0.0 0.0 0.0 12-13 1.4532238135426653E-4 0.0 0.0 0.0 0.0 14-15 2.7247946503924973E-4 0.0 0.0 0.0 0.0 16-17 3.269753580470997E-4 0.0 0.0 3.633059533856663E-5 0.0 18-19 3.8147125105494965E-4 0.0 0.0 3.633059533856663E-5 0.0 20-21 3.9963654872423297E-4 0.0 0.0 5.4495893007849944E-5 0.0 22-23 4.7229773940136625E-4 0.0 0.0 3.6330595338566633E-4 0.0 24-25 5.812895254170661E-4 0.0 0.0 7.992730974484659E-4 0.0 26-27 5.812895254170661E-4 0.0 0.0 0.001834695064597615 0.0 28-29 8.356036927870326E-4 0.0 0.0 0.004813803882360079 0.0 30-31 8.719342881255992E-4 0.0 0.0 0.015095362363174436 0.0 32-33 8.900995857948825E-4 0.0 0.0 0.03496819801337039 0.0 34-35 9.264301811334492E-4 0.0 0.0 0.06632150179055339 0.0 36-37 9.445954788027325E-4 0.0 0.0 0.1187828814594436 0.0 38-39 0.001089917860156999 0.0 0.0 0.19467749512170932 3.633059533856663E-5 40-41 0.001089917860156999 0.0 0.0 0.2787101621398139 3.633059533856663E-5 42-43 0.0011080831578262821 0.0 0.0 0.36394173880409125 3.633059533856663E-5 44-45 0.0011625790508341322 0.0 0.0 0.4703540525507529 3.633059533856663E-5 46-47 0.0012170749438419823 0.0 0.0 0.5967845243289648 3.633059533856663E-5 48-49 0.0012352402415112655 0.0 0.0 0.733423893397314 3.633059533856663E-5 50-51 0.0012352402415112655 0.0 0.0 0.8821613507134057 3.633059533856663E-5 52-53 0.0012352402415112655 0.0 0.0 1.0284646581418135 3.633059533856663E-5 54-55 0.0012715708368498321 0.0 0.0 1.1854128300044215 3.633059533856663E-5 56-57 0.0015622155995583652 0.0 0.0 1.3764027696992662 3.633059533856663E-5 58-59 0.0015622155995583652 0.0 0.0 1.579200152879145 3.633059533856663E-5 60-61 0.0015622155995583652 0.0 0.0 1.8034144220111092 3.633059533856663E-5 62-63 0.0015985461948969319 0.0 0.0 2.027628691143073 3.633059533856663E-5 64-65 0.0015985461948969319 0.0 0.0 2.2536776553396347 3.633059533856663E-5 66-67 0.0017075379809126318 0.0 0.0 2.513822883261441 3.633059533856663E-5 68-69 0.0017438685762511984 0.0 0.0 2.8048854478163676 3.633059533856663E-5 70-71 0.0017620338739204816 0.0 0.0 3.1220515451220545 3.633059533856663E-5 72-73 0.0018165297669283317 0.0 0.0 3.4463021085187617 3.633059533856663E-5 74-75 0.0018528603622668983 0.0 0.0 3.777310162648442 3.633059533856663E-5 76-77 0.0018528603622668983 0.0 0.0 4.135311849114678 3.633059533856663E-5 78-79 0.0018528603622668983 0.0 0.0 4.526955666864426 3.633059533856663E-5 80-81 0.001998182743621165 0.0 0.0 4.950788392084144 3.633059533856663E-5 82-83 0.001998182743621165 0.0 0.0 5.3773277466565865 3.633059533856663E-5 84-85 0.001998182743621165 0.0 0.0 5.81371269256578 3.633059533856663E-5 86-87 0.001998182743621165 0.0 0.0 6.265156670242808 3.633059533856663E-5 88-89 0.001998182743621165 0.0 0.0 6.741450775131417 3.633059533856663E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6735 0.0 47.644382 1 GTATCAA 11900 0.0 40.687725 1 ATCAACG 15725 0.0 30.675953 3 TATCAAC 16205 0.0 29.708122 2 TCAACGC 16190 0.0 29.706831 4 CAACGCA 16300 0.0 29.56252 5 AACGCAG 16825 0.0 28.663614 6 GTACATG 13890 0.0 26.563717 1 TACATGG 14030 0.0 26.014668 2 ACGCAGA 18975 0.0 25.287409 7 CGCAGAG 19310 0.0 24.774902 8 ACATGGG 14440 0.0 24.322033 3 GCAGAGT 20320 0.0 23.004898 9 TGGTATC 3530 0.0 22.617748 2 AGTACTT 11040 0.0 22.611475 12-13 GAGTACT 10600 0.0 22.138405 12-13 AGAGTAC 18230 0.0 21.602549 10-11 TAGCGCG 290 1.05501385E-10 21.304337 4 GTGGTAT 3910 0.0 20.821342 1 CATGGGA 8305 0.0 20.772543 4 >>END_MODULE