FastQCFastQC Report
Thu 26 May 2016
SRR938326_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938326_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2752501
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124480.45224325077447747No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100160.36388724291108343No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75790.2753495820709965No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG29280.10637598315132311No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66900.049.2666781
GTATCAA115650.039.0682641
ATCAACG152250.029.2845783
TATCAAC155300.028.848662
TCAACGC156500.028.4893114
CAACGCA158650.028.1331885
AACGCAG163400.027.3230956
GTACATG136000.026.9625931
TACATGG139500.025.9110812
ACATGGG142750.024.2741973
ACGCAGA185100.023.9146277
CGCAGAG187450.023.5894768
GAGTACT106000.021.82112712-13
GCAGAGT200650.021.658889
AGTACTT109800.021.32547212-13
AGAGTAC180450.020.740710-11
GTACTTT113200.020.43320814-15
CATGGGG61500.020.4043254
CATGGGA82350.019.624964
TAAGGTG11900.019.5723325
Produced by FastQC (version 0.11.5)