##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938326_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2752501 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.808460378397683 31.0 31.0 34.0 30.0 34.0 2 32.03285412067062 31.0 31.0 34.0 30.0 34.0 3 32.0934648161799 33.0 31.0 34.0 30.0 34.0 4 35.762131966527896 37.0 35.0 37.0 35.0 37.0 5 35.62981557499888 37.0 35.0 37.0 33.0 37.0 6 35.6626696956695 37.0 35.0 37.0 33.0 37.0 7 35.59969787476916 37.0 35.0 37.0 33.0 37.0 8 35.59497816712873 37.0 35.0 37.0 33.0 37.0 9 37.17604353277256 39.0 37.0 39.0 33.0 39.0 10-11 37.085584891703945 39.0 37.0 39.0 33.0 39.0 12-13 36.97397839274173 39.0 37.0 39.0 33.0 39.0 14-15 38.01096548193806 40.0 37.0 41.0 33.0 41.0 16-17 37.947862325935574 40.0 37.0 41.0 33.0 41.0 18-19 38.065819776268924 40.0 37.0 41.0 33.0 41.0 20-21 38.12732783748307 40.0 37.0 41.0 33.0 41.0 22-23 38.028127691870054 40.0 37.0 41.0 33.0 41.0 24-25 37.924321735032976 40.0 37.0 41.0 33.0 41.0 26-27 37.74029201079309 40.0 37.0 41.0 32.0 41.0 28-29 37.61168242990648 40.0 37.0 41.0 32.0 41.0 30-31 37.369621664079325 39.0 36.0 41.0 31.5 41.0 32-33 37.15092474080845 39.0 36.0 41.0 31.0 41.0 34-35 36.98544214879486 39.0 35.5 41.0 31.0 41.0 36-37 36.981354230207366 39.0 36.0 41.0 31.0 41.0 38-39 36.86166290221148 39.0 35.0 41.0 31.0 41.0 40-41 36.67999030699716 39.0 35.0 41.0 30.0 41.0 42-43 36.455801650934916 39.0 35.0 40.5 30.0 41.0 44-45 36.158364156815935 39.0 35.0 40.0 30.0 41.0 46-47 35.75067238849323 38.0 35.0 40.0 28.5 41.0 48-49 35.37848669264788 38.0 34.5 40.0 27.0 41.0 50-51 35.01049845213498 38.0 34.0 40.0 26.0 41.0 52-53 34.53341924308111 38.0 33.0 40.0 24.0 41.0 54-55 34.22687130722205 38.0 33.0 40.0 24.0 41.0 56-57 33.90297405886501 37.0 33.0 40.0 23.0 41.0 58-59 33.47311699432625 37.0 32.0 40.0 22.5 41.0 60-61 33.10722811726499 36.0 32.0 39.0 22.0 40.5 62-63 32.66264390094681 36.0 31.0 39.0 20.5 40.0 64-65 32.16662319105424 35.0 31.0 39.0 19.0 40.0 66-67 31.680670234088925 35.0 30.5 38.0 18.0 40.0 68-69 31.16302827864549 35.0 30.0 37.5 14.0 40.0 70-71 30.594382345365176 34.0 29.0 37.0 11.0 39.0 72-73 30.163607206682215 34.0 29.0 36.5 8.0 39.0 74-75 29.656874602407047 34.0 28.5 36.0 7.0 39.0 76-77 28.387276698537075 32.5 27.0 34.5 7.0 36.5 78-79 29.092649920926462 33.5 28.5 35.0 2.0 37.0 80-81 28.825263823700702 33.5 28.0 35.0 2.0 37.0 82-83 28.370065260648406 33.0 27.0 35.0 2.0 36.0 84-85 27.864586425218373 33.0 26.0 35.0 2.0 36.0 86-87 27.309659833002783 32.5 26.0 35.0 2.0 35.5 88-89 26.69959138979423 32.0 25.0 34.5 2.0 35.0 90-91 26.17756160669878 32.0 24.5 34.0 2.0 35.0 92-93 25.462265772110527 31.5 21.5 34.0 2.0 35.0 94-95 24.723380481968945 31.0 19.0 34.0 2.0 35.0 96-97 23.759504356220035 31.0 6.0 34.0 2.0 35.0 98-99 22.55446773679646 30.5 2.0 34.0 2.0 35.0 100-101 18.92710411367698 24.5 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2881416616953274 1101 2 0.3566602825224905 1101 3 0.3152068575501943 1101 4 0.15737076978422238 1101 5 0.13943646125261466 1101 6 0.1494954048169319 1101 7 0.24875459718392534 1101 8 0.22652921838267304 1101 9 0.26992581766000256 1101 10-11 0.25224409951912463 1101 12-13 0.27025588601711803 1101 14-15 0.37525891926458854 1101 16-17 0.39608854394471393 1101 18-19 0.3794633077763194 1101 20-21 0.40522625076682317 1101 22-23 0.34167348182636204 1101 24-25 0.3952834129318248 1101 26-27 0.49849055270494347 1101 28-29 0.4118203036261079 1101 30-31 0.4781134398210227 1101 32-33 0.3882647159883348 1101 34-35 0.3118926928322736 1101 36-37 0.5201290984593001 1101 38-39 0.6627744132743203 1101 40-41 0.535189897822697 1101 42-43 0.4836084269389076 1101 44-45 0.5424342246954268 1101 46-47 0.6534486604113852 1101 48-49 0.5887044545597107 1101 50-51 0.51413944664219 1101 52-53 0.591059553399063 1101 54-55 0.5178906253848865 1101 56-57 0.5581737501095247 1101 58-59 0.7117105399292143 1101 60-61 0.6563525149360458 1101 62-63 0.6254498293272945 1101 64-65 0.8106268992826458 1101 66-67 0.6588418531685285 1101 68-69 0.7508235358554387 1101 70-71 0.6299656990593085 1101 72-73 0.6167644800407679 1101 74-75 0.5341446416950504 1101 76-77 0.6064398256517656 1101 78-79 0.7568752271921362 1101 80-81 0.7823897232237975 1101 82-83 0.7691389711316816 1101 84-85 0.6580548671455304 1101 86-87 0.8688658584834172 1101 88-89 1.1495822359952008 1101 90-91 1.0587651701332454 1101 92-93 0.9861982749484959 1101 94-95 1.0842931391808683 1101 96-97 1.2268665771566205 1101 98-99 1.0105248659877226 1101 100-101 0.7352006486837119 1102 1 0.3324507079845773 1102 2 0.3220165104529755 1102 3 0.3096292228636486 1102 4 0.2392001834948232 1102 5 0.21367896242580287 1102 6 0.2116948555312348 1102 7 0.2791653305649504 1102 8 0.274882361389281 1102 9 0.33225328117558206 1102 10-11 0.37820708043260254 1102 12-13 0.3236246844354227 1102 14-15 0.3980679694003655 1102 16-17 0.37700694347697095 1102 18-19 0.33977349264095835 1102 20-21 0.34370017508233275 1102 22-23 0.3952512242159898 1102 24-25 0.3694819866353498 1102 26-27 0.2729291504108673 1102 28-29 0.227369781650836 1102 30-31 0.3352964658159294 1102 32-33 0.2540837594311398 1102 34-35 0.2787944599320582 1102 36-37 0.28138830130266257 1102 38-39 0.24805447974250683 1102 40-41 0.4126791082822052 1102 42-43 0.3892606844723545 1102 44-45 0.3662272193795104 1102 46-47 0.23662274705082353 1102 48-49 0.35782944398172845 1102 50-51 0.3370251967556612 1102 52-53 0.2690131949170649 1102 54-55 0.1417516268696417 1102 56-57 0.24961550967010027 1102 58-59 0.21255393196072703 1102 60-61 0.3548887531752527 1102 62-63 0.20964301787691397 1102 64-65 0.3536845896670755 1102 66-67 0.504005984966323 1102 68-69 0.18925684072438287 1102 70-71 0.3860525385124838 1102 72-73 0.20891238713917204 1102 74-75 0.12033395293386917 1102 76-77 0.042349031957012784 1102 78-79 0.1019621974276177 1102 80-81 0.11656775641172956 1102 82-83 0.03352864086788898 1102 84-85 -0.006896781865524559 1102 86-87 -0.17085865242921017 1102 88-89 0.05028707570993873 1102 90-91 0.07370858755190923 1102 92-93 -0.1733999925705625 1102 94-95 -0.1487843224114478 1102 96-97 -0.048234932994397184 1102 98-99 -0.15434715967638013 1102 100-101 -0.42720151460953915 1103 1 0.2753647758986446 1103 2 0.26717495561142357 1103 3 0.32393548716991205 1103 4 0.19802176731641197 1103 5 0.18616630991315475 1103 6 0.12315089198727236 1103 7 0.20470188110150644 1103 8 0.22202530853222413 1103 9 0.28268010660240606 1103 10-11 0.29635293607845625 1103 12-13 0.252836238646978 1103 14-15 0.48722618355858316 1103 16-17 0.4356705021405247 1103 18-19 0.4722311250985882 1103 20-21 0.39419735057950334 1103 22-23 0.5243921658569306 1103 24-25 0.5074678746212413 1103 26-27 0.48145148393320625 1103 28-29 0.5107178149988627 1103 30-31 0.44021650823597014 1103 32-33 0.5856465284939034 1103 34-35 0.4967261153637139 1103 36-37 0.4734361183504774 1103 38-39 0.4504461196341438 1103 40-41 0.5561446892477875 1103 42-43 0.48398915420082034 1103 44-45 0.4441057972580893 1103 46-47 0.4416183895464627 1103 48-49 0.503518402170684 1103 50-51 0.4779655251959909 1103 52-53 0.6315221199259895 1103 54-55 0.5805877532057693 1103 56-57 0.7472149447695315 1103 58-59 0.6447501141569063 1103 60-61 0.7086406694271687 1103 62-63 0.5510193592532602 1103 64-65 0.5561602296427139 1103 66-67 0.5463433398036805 1103 68-69 0.5329510594186004 1103 70-71 0.24295970791965615 1103 72-73 0.10684178506857123 1103 74-75 0.500313582913499 1103 76-77 0.29653459864258025 1103 78-79 0.405457125922549 1103 80-81 0.41873312107709637 1103 82-83 0.3740557938950424 1103 84-85 0.38787736290862185 1103 86-87 0.6372602181896596 1103 88-89 0.5753926008154657 1103 90-91 0.535718237061559 1103 92-93 0.4888448571742856 1103 94-95 0.528715730088603 1103 96-97 0.6742261644666989 1103 98-99 0.5755230076937856 1103 100-101 0.3506537007456707 1104 1 0.22379502182797495 1104 2 0.2445963124757995 1104 3 0.27059311951827425 1104 4 0.20651262350789068 1104 5 0.19484616052228176 1104 6 0.1395619072531744 1104 7 0.18034017054521456 1104 8 0.17018086606850602 1104 9 0.36777807558641484 1104 10-11 0.34773514001196304 1104 12-13 0.32833758279068803 1104 14-15 0.4722514708466008 1104 16-17 0.45022609841103645 1104 18-19 0.38541253663104413 1104 20-21 0.33516548067369456 1104 22-23 0.3669945474616938 1104 24-25 0.32373724035927154 1104 26-27 0.4595483514126286 1104 28-29 0.399767362897812 1104 30-31 0.49613512839516005 1104 32-33 0.5425345497140981 1104 34-35 0.43070537219474403 1104 36-37 0.5510042966423683 1104 38-39 0.4750568654110694 1104 40-41 0.5244815906416704 1104 42-43 0.49461176838700993 1104 44-45 0.4253763679386964 1104 46-47 0.45555438948924376 1104 48-49 0.5511557931343845 1104 50-51 0.5937007409907551 1104 52-53 0.5179838610735104 1104 54-55 0.5544250248856599 1104 56-57 0.6148948498486675 1104 58-59 0.6424832815492891 1104 60-61 0.482820194316659 1104 62-63 0.5903731138873383 1104 64-65 0.6802541155770214 1104 66-67 0.5833062600342807 1104 68-69 0.629021983769114 1104 70-71 0.4724562207388132 1104 72-73 0.41440282723935695 1104 74-75 0.37312776756351695 1104 76-77 0.24158075784685806 1104 78-79 0.2662027652958905 1104 80-81 0.3747316030695309 1104 82-83 0.3040508998474607 1104 84-85 0.3440768749894083 1104 86-87 0.5184595514547681 1104 88-89 0.2468856402088342 1104 90-91 0.23397496300699672 1104 92-93 0.4273151385586367 1104 94-95 0.48113026444707785 1104 96-97 0.4479933816655759 1104 98-99 0.5104078971636916 1104 100-101 0.10118485208755956 1105 1 0.27205040661606716 1105 2 0.24463615958996598 1105 3 0.17902431877607938 1105 4 0.15050532023348495 1105 5 0.1051329644289396 1105 6 0.22505011780268092 1105 7 0.22349777236849633 1105 8 0.20953485565159724 1105 9 0.15326239409220932 1105 10-11 0.24966047049148443 1105 12-13 0.29982650926442034 1105 14-15 0.3858585587082928 1105 16-17 0.22687554388747344 1105 18-19 0.28954157138302605 1105 20-21 0.23839649915004202 1105 22-23 0.1763210094404002 1105 24-25 0.25857284519874923 1105 26-27 0.1733453286449631 1105 28-29 0.22457111917009343 1105 30-31 0.26086790385124203 1105 32-33 0.2886291353782511 1105 34-35 0.3115841453590207 1105 36-37 0.3580797938178506 1105 38-39 0.21918179895508416 1105 40-41 0.2567394313887732 1105 42-43 0.23943806409337043 1105 44-45 0.3591418593317286 1105 46-47 0.1479587617105338 1105 48-49 0.012334841442324773 1105 50-51 -0.1781955130098396 1105 52-53 -0.023085341581769114 1105 54-55 -0.03337067776855207 1105 56-57 -0.005661667391763103 1105 58-59 0.09276387936719743 1105 60-61 0.045518370827991816 1105 62-63 0.2210823051471209 1105 64-65 0.10747653872636675 1105 66-67 -0.038051568110013534 1105 68-69 -0.13410671367240923 1105 70-71 -0.1582059085638825 1105 72-73 -0.21558070968051268 1105 74-75 -0.18290323216748305 1105 76-77 -0.20976975484385108 1105 78-79 -0.16561955046482169 1105 80-81 0.04112176694840031 1105 82-83 0.04931975004772937 1105 84-85 0.1530686741620677 1105 86-87 -0.04923950407595967 1105 88-89 -0.018824448603897537 1105 90-91 -0.1219160952476308 1105 92-93 0.06837398890717239 1105 94-95 -0.03493209403609754 1105 96-97 0.06301849726192188 1105 98-99 -0.2935588631944448 1105 100-101 -0.306059009073973 1106 1 0.08224644274271498 1106 2 0.07529080508065533 1106 3 0.16542547339673774 1106 4 0.07317740093068892 1106 5 0.030489553672488512 1106 6 -0.00525611329943132 1106 7 0.056059280391231425 1106 8 0.07732636684080063 1106 9 -0.03205742899978503 1106 10-11 0.026693305986498217 1106 12-13 0.046361638375387315 1106 14-15 0.1402063656740964 1106 16-17 0.014737216545583465 1106 18-19 0.2485251419941079 1106 20-21 0.1268579112475834 1106 22-23 0.23240717603359684 1106 24-25 0.1627257103302 1106 26-27 0.17503268597305777 1106 28-29 0.2665601234727575 1106 30-31 0.1532581615971722 1106 32-33 0.22454771485750058 1106 34-35 0.03580728837345504 1106 36-37 -0.23947429474790738 1106 38-39 -0.015664257660439773 1106 40-41 0.1524337145781658 1106 42-43 0.26591702729410827 1106 44-45 0.2627208677528614 1106 46-47 -0.2872474929848323 1106 48-49 -0.1968379020390003 1106 50-51 -0.0407651415609962 1106 52-53 -0.08795857182914801 1106 54-55 -0.25219945129196475 1106 56-57 0.014475962500476669 1106 58-59 -0.05637367343305044 1106 60-61 -0.29680187031988225 1106 62-63 -0.28158338312392317 1106 64-65 -0.5791553356540575 1106 66-67 -0.32207791442584366 1106 68-69 -0.8780494953789457 1106 70-71 -0.6147357291930753 1106 72-73 -0.2521017449275931 1106 74-75 -0.10727198331868593 1106 76-77 -0.34227949740835584 1106 78-79 -0.3417749342794387 1106 80-81 -0.9650386071495198 1106 82-83 -0.4151705296357946 1106 84-85 -0.3347399912876803 1106 86-87 0.01805998949695109 1106 88-89 -0.06985468728897004 1106 90-91 -0.15302562226500882 1106 92-93 -0.30325343069468147 1106 94-95 -0.3658174980912392 1106 96-97 -0.4554976786030096 1106 98-99 -0.7945928788694694 1106 100-101 -0.831867233185033 1107 1 0.11475337264018037 1107 2 0.1456724924800028 1107 3 0.1340112259786821 1107 4 0.15938005390093934 1107 5 0.0774225592010751 1107 6 0.11873954538135223 1107 7 0.1250800995566479 1107 8 0.09881961066589184 1107 9 0.152875092747621 1107 10-11 0.148463596164234 1107 12-13 0.12245267896477685 1107 14-15 0.21427915891472793 1107 16-17 0.30452613117715543 1107 18-19 0.1884112801927671 1107 20-21 0.14914673957866853 1107 22-23 0.19181853322673703 1107 24-25 0.2859851667131963 1107 26-27 0.2359258068464456 1107 28-29 0.2593278939256862 1107 30-31 0.29517808746225427 1107 32-33 0.43752801753603165 1107 34-35 0.388497476874889 1107 36-37 0.2671755155966622 1107 38-39 0.24731087945139762 1107 40-41 0.2788178714797951 1107 42-43 0.3321335481596961 1107 44-45 0.5273019415402018 1107 46-47 0.6162043268586501 1107 48-49 0.5066189400562919 1107 50-51 0.3718012552873802 1107 52-53 0.5742338666535716 1107 54-55 0.4914142819621645 1107 56-57 0.47516899087968767 1107 58-59 0.21856514046061193 1107 60-61 0.3178045452765659 1107 62-63 0.35304536716161294 1107 64-65 0.5051083293261058 1107 66-67 0.4020808864348915 1107 68-69 0.3470644679890249 1107 70-71 0.36000894912048054 1107 72-73 0.18681620780317232 1107 74-75 0.2547577242354677 1107 76-77 0.2944005249587356 1107 78-79 0.3622926161290785 1107 80-81 0.3667866174903196 1107 82-83 0.21741941073150883 1107 84-85 0.3615307935756249 1107 86-87 0.32871505189562455 1107 88-89 0.2550302962902613 1107 90-91 0.26129538486721415 1107 92-93 0.19082000060870286 1107 94-95 0.12194771076402233 1107 96-97 0.20638576054032143 1107 98-99 -0.0031062437295830136 1107 100-101 -0.1357304165773563 1108 1 0.2070965420526889 1108 2 0.30753271364456225 1108 3 0.19446557709477474 1108 4 0.21286071217598845 1108 5 0.17133562640984223 1108 6 0.14060249076624132 1108 7 0.24733822445999465 1108 8 0.24033040868051359 1108 9 0.30039090543425573 1108 10-11 0.370711080330004 1108 12-13 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-0.39705303972732153 2316 72-73 -0.10963694198062868 2316 74-75 0.09839841270973437 2316 76-77 -0.012449469317996176 2316 78-79 -0.04348426513293546 2316 80-81 -0.2451353614994396 2316 82-83 -0.3214873163292751 2316 84-85 -0.07421073189759753 2316 86-87 0.029493474936337805 2316 88-89 0.19883567476189157 2316 90-91 0.10249398932388587 2316 92-93 0.019521087095480283 2316 94-95 0.013214994453640827 2316 96-97 0.2383846182224687 2316 98-99 0.22652493243732152 2316 100-101 0.3772904885401793 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 15.0 4 60.0 5 129.0 6 232.0 7 612.0 8 1299.0 9 2214.0 10 3604.0 11 5655.0 12 7989.0 13 11226.0 14 14836.0 15 19174.0 16 24543.0 17 31030.0 18 29092.0 19 22850.0 20 22290.0 21 23831.0 22 25388.0 23 29276.0 24 33844.0 25 40152.0 26 47425.0 27 57256.0 28 68944.0 29 82629.0 30 98678.0 31 117239.0 32 141709.0 33 174792.0 34 223768.0 35 294937.0 36 391129.0 37 458703.0 38 235803.0 39 10144.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.60259838400279 19.25220165087485 15.118874614892752 23.026325350229612 2 15.977939703844196 20.788735916533483 38.990006858857654 24.243317520764673 3 19.20985237639025 24.620513988693077 29.777621796835064 26.392011838081608 4 13.363482883384965 17.136451539890448 35.762675472234164 33.73739010449043 5 14.126080708409452 37.330221145230915 33.31149129033384 15.232206856025785 6 32.54291279094903 35.735718170492945 16.97318184443893 14.748187194119094 7 29.263422610927297 32.613176162333815 19.819429675048255 18.303971551690626 8 26.890308123412126 34.72819810056381 19.329220952144976 19.052272823879083 9 27.067964734617718 16.44980328799154 19.631709488933883 36.85052248845686 10-11 25.51746575205604 26.640862255817527 26.47817384989143 21.363498142235006 12-13 26.363968623444638 23.855631660079325 27.24607547826504 22.534324238210996 14-15 22.984732793920873 25.092452282487816 25.828600970535526 26.094213953055785 16-17 22.57341595879529 28.576956012005084 26.27009036508979 22.579537664109843 18-19 22.56894729556865 26.49405032005438 29.15964426534268 21.77735811903429 20-21 23.25305240579386 25.658918925006745 29.136465345516676 21.951563323682716 22-23 22.894505760397543 25.641589231030252 29.054703340707235 22.40920166786497 24-25 22.784310234895976 25.866186859558326 29.087173637009332 22.262329268536366 26-27 22.354440907349364 26.333354951942194 28.947498935873938 22.364705204834504 28-29 22.26375212942702 26.420208385028744 29.19067059376182 22.12536889178242 30-31 22.241208995019438 26.048837039477913 29.07957163321648 22.630382332286164 32-33 22.151308936854157 26.174758882921388 28.863331929761333 22.810600250463125 34-35 22.899643921226655 26.152732359566894 28.610947605551925 22.33667611365453 36-37 22.825316707711192 25.81418300500436 28.695147336138422 22.665352951146023 38-39 22.354771887876897 26.224051827042395 28.899403808078482 22.521772477002223 40-41 22.65670259528611 25.948156043069293 28.882315529371116 22.512825832273485 42-43 22.619889928895383 25.956809707270878 28.5686233228162 22.854677041017535 44-45 22.496649410844903 26.314595344379534 28.374594595969267 22.814160648806304 46-47 22.573888256534694 26.595067540393263 27.886947180037353 22.944097023034686 48-49 22.7546692989394 26.543605252096185 27.868073435758973 22.833652013205445 50-51 22.74705803921597 26.68340538295899 27.625348728302008 22.944187849523033 52-53 23.088892610756545 26.643677876956268 27.427419644897494 22.840009867389693 54-55 22.512035417970782 26.580553467555507 27.7079100062089 23.199501108264812 56-57 22.657030823967002 26.969799466012912 27.529090815952472 22.844078894067614 58-59 22.78693086760005 26.42438640349268 27.585821040573645 23.202861688333627 60-61 22.7123259900723 26.52295130864621 27.865330475810907 22.89939222547058 62-63 22.54871115396507 26.71432271959211 27.76640226470399 22.97056386173883 64-65 22.3136158715292 26.42080783985183 27.736011721703278 23.52956456691569 66-67 22.362292890516418 26.778297325757915 27.523547234068523 23.335862549657136 68-69 22.436265985388783 26.527221233112243 27.65698646799453 23.37952631350445 70-71 22.34425474010077 26.72385589897473 27.417352846022315 23.514536514902183 72-73 22.60108170714561 26.582170179048077 27.33459497380746 23.48215313999886 74-75 22.38992828703786 26.798736857861265 27.184622276249854 23.626712578851016 76-77 22.37503274294905 26.607383612213038 27.471870128294228 23.54571351654368 78-79 22.434251613350913 26.69702935621095 27.411198034078826 23.45752099635931 80-81 22.19312104002757 27.036397004794427 27.28579159302814 23.484690362149863 82-83 22.415617476672406 26.84996315141924 27.097775987877114 23.636643384031238 84-85 22.29760403668345 26.542672402595812 27.165865202516315 23.993858358204427 86-87 22.388436300723612 26.483710277531834 27.247541364192728 23.880312057551826 88-89 22.165748289100197 26.584787133437647 27.18980097722106 24.05966360024109 90-91 22.098300857337232 26.614458017361102 27.291808019677248 23.99543310562442 92-93 22.21980419818222 26.301945484013128 27.286567698839992 24.191682618964663 94-95 21.97458274757807 26.170858097936765 27.303428282755988 24.55113087172918 96-97 22.156449716094563 26.10622121481518 27.25119809220778 24.486130976882478 98-99 21.925972402184847 26.165463003548954 27.05434567586818 24.854218918398015 100-101 21.944733171759065 25.925639990684836 27.374703951061235 24.754922886494864 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1364.0 1 901.0 2 637.5 3 885.0 4 766.0 5 734.5 6 1339.0 7 2883.5 8 4392.5 9 4814.0 10 4099.5 11 3270.0 12 3219.5 13 3398.5 14 3758.0 15 4001.0 16 4114.0 17 4261.5 18 4430.0 19 4652.5 20 5041.5 21 5788.0 22 6661.5 23 7751.0 24 9119.0 25 10842.0 26 13105.5 27 15990.5 28 18743.0 29 21730.0 30 25893.0 31 30451.5 32 35542.0 33 40009.5 34 43498.0 35 47455.0 36 52522.5 37 57940.5 38 62578.5 39 65417.5 40 68394.0 41 75904.5 42 84273.0 43 91294.0 44 96154.0 45 98736.0 46 99675.0 47 102852.0 48 111650.0 49 116295.0 50 116061.5 51 113420.0 52 107812.5 53 105890.0 54 105074.5 55 96293.5 56 84180.0 57 75116.5 58 67493.0 59 59478.5 60 50166.5 61 41260.5 62 33897.0 63 26248.0 64 20103.0 65 16070.0 66 12300.0 67 10031.0 68 7676.5 69 5121.0 70 4043.5 71 3414.5 72 2978.5 73 2650.0 74 2211.0 75 1898.0 76 1341.5 77 717.0 78 411.0 79 256.0 80 148.0 81 94.5 82 47.5 83 26.0 84 11.5 85 4.0 86 2.5 87 1.5 88 1.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.629425021098993E-4 2 0.04773840227487656 3 0.0010172566694798659 4 0.0 5 0.08911895036550396 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.179835720313998E-4 26-27 0.008810169369602409 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010935509196908557 36-37 9.082648834641658E-5 38-39 0.004196183761604446 40-41 0.005286101621761445 42-43 2.724794650392498E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.449589300784995E-5 68-69 9.627607764720157E-4 70-71 5.449589300784995E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0012897361345191156 82-83 7.44777204440616E-4 84-85 0.005177109835745745 86-87 0.0021798357203139983 88-89 7.266119067713328E-5 90-91 0.003033604710770314 92-93 8.719342881255992E-4 94-95 1.816529766928332E-5 96-97 0.0 98-99 1.816529766928332E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2752501.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.01417393099392 #Duplication Level Percentage of deduplicated Percentage of total 1 70.35486694137361 30.262564835081797 2 14.940797299853095 12.853321074472108 3 5.76214164815853 7.43561289206745 4 2.8121948957895415 4.838569615013786 5 1.597621748685697 3.4360189886952615 6 0.9721467398904129 2.508965337365696 7 0.639226088418249 1.9247047503916024 8 0.4530272049542636 1.5589272791499782 9 0.3434915709235964 1.329750555798412 >10 1.8411137616299877 14.435704172967533 >50 0.16012573055476664 4.766796207662746 >100 0.11157923941489344 9.316123376630927 >500 0.008623531130805263 2.5042777178337294 >1k 0.0027899659540787107 1.7361169023889063 >5k 8.454442285087002E-5 0.27561855892763976 >10k+ 1.6908884570174005E-4 0.8169277355522496 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12448 0.45224325077447747 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10016 0.36388724291108343 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7579 0.2753495820709965 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2928 0.10637598315132311 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 7.266119067713326E-5 0.0 0.0 0.0 0.0 3 7.266119067713326E-5 0.0 0.0 0.0 0.0 4 7.266119067713326E-5 0.0 0.0 0.0 0.0 5 7.266119067713326E-5 0.0 0.0 0.0 0.0 6 1.089917860156999E-4 0.0 0.0 0.0 0.0 7 1.8165297669283317E-4 0.0 0.0 0.0 0.0 8 1.8165297669283317E-4 0.0 0.0 0.0 0.0 9 1.8165297669283317E-4 0.0 0.0 3.633059533856663E-5 0.0 10-11 1.8165297669283317E-4 0.0 0.0 3.633059533856663E-5 0.0 12-13 2.543141673699664E-4 0.0 0.0 3.633059533856663E-5 0.0 14-15 3.088100603778164E-4 0.0 0.0 3.633059533856663E-5 0.0 16-17 3.45140655716383E-4 0.0 0.0 7.266119067713326E-5 0.0 18-19 3.8147125105494965E-4 0.0 0.0 7.266119067713326E-5 0.0 20-21 3.9963654872423297E-4 0.0 0.0 9.082648834641658E-5 0.0 22-23 4.359671440627996E-4 0.0 0.0 3.9963654872423297E-4 0.0 24-25 5.086283347399328E-4 0.0 0.0 8.356036927870326E-4 0.0 26-27 5.267936324092162E-4 0.0 0.0 0.0018710256599361816 0.0 28-29 6.357854184249161E-4 0.0 0.0 0.004886465073037212 0.0 30-31 6.90281311432766E-4 0.0 0.0 0.015240684744528703 0.0 32-33 6.90281311432766E-4 0.0 0.0 0.035295173371417486 0.0 34-35 7.266119067713327E-4 0.0 0.0 0.06684829542296261 0.0 36-37 9.445954788027325E-4 0.0 0.0 0.11956398925922279 0.0 38-39 0.0010172566694798657 0.0 0.0 0.19578557827953558 0.0 40-41 0.0010354219671491489 0.0 0.0 0.2804540307160651 0.0 42-43 0.0011807443485034154 0.0 0.0 0.3661215745244053 0.0 44-45 0.0013442320275269654 0.0 0.0 0.47284269833144477 0.0 46-47 0.0014713891112119485 0.0 0.0 0.5997091372537194 0.0 48-49 0.0015258850042197986 0.0 0.0 0.7357127209036436 0.0 50-51 0.0015258850042197986 0.0 0.0 0.8848316494707904 0.0 52-53 0.0015258850042197986 0.0 0.0 1.031825238210631 0.0 54-55 0.0015622155995583652 0.0 0.0 1.1889550630499317 0.0 56-57 0.0016348767902354985 0.0 0.0 1.380035829233123 0.0 58-59 0.0016348767902354985 0.0 0.0 1.582324584078262 0.0 60-61 0.0016348767902354985 0.0 0.0 1.8057940760057853 0.0 62-63 0.0016348767902354985 0.0 0.0 2.0299720145424107 0.0 64-65 0.0016348767902354985 0.0 0.0 2.255802995166941 0.0 66-67 0.0016712073855740652 0.0 0.0 2.5147493134425747 0.0 68-69 0.0017438685762511984 0.0 0.0 2.8052669190674226 0.0 70-71 0.001780199171589765 0.0 0.0 3.1223785204801016 0.0 72-73 0.001834695064597615 0.0 0.0 3.447719001736966 0.0 74-75 0.0018528603622668983 0.0 0.0 3.7814518505170387 0.0 76-77 0.0018528603622668983 0.0 0.0 4.143304580089163 0.0 78-79 0.0018528603622668983 0.0 0.0 4.53801833314502 0.0 80-81 0.001998182743621165 0.0 0.0 4.966901011116799 0.0 82-83 0.0021071745296368646 0.0 0.0 5.398799128501679 0.0 84-85 0.002125339827306148 0.0 0.0 5.840524671925641 0.0 86-87 0.0021435051249754315 0.0 0.0 6.298290173191582 0.0 88-89 0.0021435051249754315 0.0 0.0 6.7834489433428 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6690 0.0 49.266678 1 GTATCAA 11565 0.0 39.068264 1 ATCAACG 15225 0.0 29.284578 3 TATCAAC 15530 0.0 28.84866 2 TCAACGC 15650 0.0 28.489311 4 CAACGCA 15865 0.0 28.133188 5 AACGCAG 16340 0.0 27.323095 6 GTACATG 13600 0.0 26.962593 1 TACATGG 13950 0.0 25.911081 2 ACATGGG 14275 0.0 24.274197 3 ACGCAGA 18510 0.0 23.914627 7 CGCAGAG 18745 0.0 23.589476 8 GAGTACT 10600 0.0 21.821127 12-13 GCAGAGT 20065 0.0 21.65888 9 AGTACTT 10980 0.0 21.325472 12-13 AGAGTAC 18045 0.0 20.7407 10-11 GTACTTT 11320 0.0 20.433208 14-15 CATGGGG 6150 0.0 20.404325 4 CATGGGA 8235 0.0 19.62496 4 TAAGGTG 1190 0.0 19.572332 5 >>END_MODULE