FastQCFastQC Report
Thu 26 May 2016
SRR938325_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938325_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750543
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136440.49604750771029577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104250.3790160706449599No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72000.26176649483392916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66750.049.1147961
GTATCAA119600.042.1531141
ATCAACG155800.032.0582163
TATCAAC157750.031.6920592
TCAACGC159900.031.206494
CAACGCA161500.030.8384725
AACGCAG166450.029.9191996
GTACATG142600.026.933371
ACGCAGA188450.026.2698237
TACATGG145700.026.0610142
CGCAGAG189950.025.9623288
ACATGGG149650.024.2616713
GCAGAGT200050.023.866979
GAGTACT101850.022.7369412-13
AGTACTT105400.021.99366612-13
AGAGTAC179450.021.37614410-11
GTACTTT109400.021.12495614-15
TGGTATC32750.021.040732
CATGGGG70950.020.8980754
CATGGGA84900.020.5989074
Produced by FastQC (version 0.11.5)