##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938325_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2750543 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.28289941295228 31.0 31.0 34.0 28.0 34.0 2 31.43045936747762 31.0 31.0 34.0 28.0 34.0 3 31.48784440017844 33.0 31.0 34.0 28.0 34.0 4 35.15997568480115 37.0 35.0 37.0 32.0 37.0 5 35.17391693203851 37.0 35.0 37.0 32.0 37.0 6 35.187663308663055 37.0 35.0 37.0 32.0 37.0 7 35.105771115012566 37.0 35.0 37.0 32.0 37.0 8 35.06679771957755 37.0 35.0 37.0 32.0 37.0 9 36.57886388251338 39.0 37.0 39.0 32.0 39.0 10-11 36.44458166987391 39.0 37.0 39.0 32.0 39.0 12-13 36.283211533140914 39.0 35.0 39.0 32.0 39.0 14-15 37.30143793425516 39.5 36.5 41.0 32.0 41.0 16-17 37.1525593310121 39.0 36.0 41.0 31.0 41.0 18-19 37.03870363051951 39.0 36.0 41.0 31.0 41.0 20-21 37.03871671884424 39.0 36.0 41.0 31.0 41.0 22-23 37.12654264994221 39.0 36.0 41.0 31.0 41.0 24-25 37.05132586547457 39.0 36.0 41.0 31.0 41.0 26-27 36.87719915667561 39.0 36.0 41.0 30.0 41.0 28-29 36.694545767872015 39.0 36.0 41.0 30.0 41.0 30-31 36.42523858016399 39.0 35.5 40.5 30.0 41.0 32-33 36.138230160371975 38.5 35.0 40.0 29.5 41.0 34-35 35.87908187583325 38.0 35.0 40.0 29.0 41.0 36-37 35.60451045484473 38.0 35.0 40.0 27.0 41.0 38-39 35.30536770375886 38.0 34.0 40.0 26.5 41.0 40-41 35.20082398275541 38.0 34.0 40.0 26.0 41.0 42-43 34.97177193739563 38.0 34.0 40.0 25.5 41.0 44-45 34.64358837509539 38.0 33.5 40.0 24.0 41.0 46-47 34.21932778364126 38.0 33.0 40.0 23.5 41.0 48-49 33.82946149178544 38.0 33.0 40.0 21.5 41.0 50-51 33.03701523662782 37.0 32.0 39.5 19.5 40.5 52-53 33.05667390038985 37.0 32.0 39.5 19.5 40.5 54-55 33.14339186844198 37.0 32.0 40.0 18.5 41.0 56-57 32.84288665910695 37.0 31.5 40.0 16.0 41.0 58-59 32.43557672066933 36.5 31.0 39.5 12.5 41.0 60-61 31.971492356236567 36.0 31.0 39.0 8.0 41.0 62-63 31.41392754085284 35.5 30.0 39.0 7.0 40.0 64-65 30.93380198019082 35.0 29.0 39.0 2.0 40.0 66-67 30.39453118893251 35.0 29.0 38.0 2.0 40.0 68-69 29.81179952467567 34.5 28.0 37.5 2.0 40.0 70-71 29.193058425190955 34.0 27.5 37.0 2.0 39.0 72-73 28.570059439172557 34.0 26.0 36.0 2.0 39.0 74-75 27.886181564876463 33.5 26.0 36.0 2.0 39.0 76-77 27.181340375336795 33.0 24.5 35.0 2.0 37.5 78-79 26.417703886105397 32.0 22.5 35.0 2.0 37.0 80-81 25.682113495407997 32.0 20.0 35.0 2.0 36.5 82-83 24.984820997163105 31.0 18.0 35.0 2.0 36.0 84-85 24.264608660908046 31.0 10.5 34.0 2.0 35.5 86-87 23.550708714606536 30.5 4.5 34.0 2.0 35.0 88-89 22.809622499993637 30.0 2.0 34.0 2.0 35.0 90-91 22.020440327600767 29.0 2.0 34.0 2.0 35.0 92-93 21.160946584001778 29.0 2.0 34.0 2.0 35.0 94-95 20.26194355078252 28.0 2.0 34.0 2.0 35.0 96-97 19.205853716884267 26.0 2.0 34.0 2.0 35.0 98-99 17.965407739489983 24.5 2.0 33.0 2.0 35.0 100-101 15.071534420658029 2.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5634716350414415 1101 2 0.5235917832138384 1101 3 0.5951662340302306 1101 4 0.41608575137026094 1101 5 0.47389884265806614 1101 6 0.3694565585653393 1101 7 0.3346372691605737 1101 8 0.2572426997917745 1101 9 0.47263508558841494 1101 10-11 0.5801309217396309 1101 12-13 0.6076218714204273 1101 14-15 0.7268601895200604 1101 16-17 0.7913761305843465 1101 18-19 0.831437787329314 1101 20-21 0.7834705089951299 1101 22-23 0.7824453287113826 1101 24-25 0.7850216224841873 1101 26-27 0.8881905570564257 1101 28-29 0.8745055079063064 1101 30-31 0.8008550464073849 1101 32-33 0.9470854908856126 1101 34-35 0.9514662684229691 1101 36-37 0.9939765317575038 1101 38-39 1.0800933819125902 1101 40-41 1.2661914366123241 1101 42-43 1.2972300048900038 1101 44-45 1.2950282997062459 1101 46-47 1.3340562613387945 1101 48-49 1.2248786975196069 1101 50-51 1.223946377372279 1101 52-53 1.338063139644774 1101 54-55 1.53965540623404 1101 56-57 1.6431955804722804 1101 58-59 1.7195013987378402 1101 60-61 1.7647747460058447 1101 62-63 1.7930615891479142 1101 64-65 1.7489256743012938 1101 66-67 1.8562837958338605 1101 68-69 2.0304612388273284 1101 70-71 2.0243643897353003 1101 72-73 2.2318712414172097 1101 74-75 2.4403857190311236 1101 76-77 2.7113934261282466 1101 78-79 2.8554402333715423 1101 80-81 3.2304254945782986 1101 82-83 3.4490429363711286 1101 84-85 3.567797427023077 1101 86-87 3.718516402901482 1101 88-89 4.131608081948627 1101 90-91 4.42191299143477 1101 92-93 4.564565457309872 1101 94-95 4.731959394855121 1101 96-97 4.943507721053724 1101 98-99 5.270221116908569 1101 100-101 5.312809410504164 1102 1 0.5854038275457256 1102 2 0.5931883743538187 1102 3 0.5577299737056194 1102 4 0.45243642446184396 1102 5 0.3609855487823026 1102 6 0.45502927839675067 1102 7 0.5134499551013789 1102 8 0.522851186254627 1102 9 0.624234760859423 1102 10-11 0.5967754214055248 1102 12-13 0.672210721678745 1102 14-15 1.0258201584526532 1102 16-17 0.9684194284963112 1102 18-19 1.0767341361447151 1102 20-21 0.8977358982559096 1102 22-23 0.7516240314025566 1102 24-25 0.7834109630136155 1102 26-27 0.8604314682041831 1102 28-29 0.9065910575577902 1102 30-31 0.8905701385753559 1102 32-33 0.8971167782857421 1102 34-35 0.8319873564056479 1102 36-37 1.0059867380496357 1102 38-39 0.9577463563696682 1102 40-41 1.1413891598428805 1102 42-43 1.3251960213959322 1102 44-45 1.4556858911936246 1102 46-47 1.5139139739426355 1102 48-49 1.3416781675892722 1102 50-51 1.54986652178318 1102 52-53 1.5792293377642963 1102 54-55 1.6340729274225438 1102 56-57 1.6697437338893195 1102 58-59 1.8462084182066079 1102 60-61 1.8455362642147044 1102 62-63 2.097519539523777 1102 64-65 2.184917122819318 1102 66-67 2.0864509756566676 1102 68-69 2.133694611361573 1102 70-71 2.2492981866354604 1102 72-73 2.338987123653144 1102 74-75 2.481947012239587 1102 76-77 2.592872614354647 1102 78-79 2.8091902604883074 1102 80-81 3.189191588569436 1102 82-83 3.486823617807037 1102 84-85 3.6951974899034177 1102 86-87 3.858404003527596 1102 88-89 4.116710054735311 1102 90-91 4.3687561874793595 1102 92-93 4.320521368352214 1102 94-95 4.470917066119263 1102 96-97 4.666928326067712 1102 98-99 5.193362503264272 1102 100-101 5.1641045835977355 1103 1 0.6037402169439581 1103 2 0.6197728575944659 1103 3 0.5457161603797758 1103 4 0.4129416530235659 1103 5 0.41957099106292617 1103 6 0.4310524038607966 1103 7 0.4986570259211476 1103 8 0.41873365835518683 1103 9 0.6383687875716646 1103 10-11 0.6710863629929023 1103 12-13 0.704502411588571 1103 14-15 0.7687687863735775 1103 16-17 0.8002636685030566 1103 18-19 0.8139197738209916 1103 20-21 0.9114035305253338 1103 22-23 0.8635967617657556 1103 24-25 0.7826509938324051 1103 26-27 0.8800375355321108 1103 28-29 0.9921586871583514 1103 30-31 0.9124869584827948 1103 32-33 0.9705378077749103 1103 34-35 1.084382440625724 1103 36-37 1.0683337433904185 1103 38-39 1.1253718442571952 1103 40-41 1.287963238899799 1103 42-43 1.4791344074524062 1103 44-45 1.4101708687861247 1103 46-47 1.585396578873727 1103 48-49 1.6597233010957737 1103 50-51 1.89614470725504 1103 52-53 1.938470094863355 1103 54-55 2.0737752575134323 1103 56-57 2.1196618961026985 1103 58-59 2.313395862833211 1103 60-61 2.4682345354488895 1103 62-63 2.5104013452840555 1103 64-65 2.5197920516679098 1103 66-67 2.553058271098294 1103 68-69 2.795905154763606 1103 70-71 3.0365260666977143 1103 72-73 2.9861612589749136 1103 74-75 3.2014107548843604 1103 76-77 3.3257727608795093 1103 78-79 3.281252752093426 1103 80-81 3.707253435272669 1103 82-83 3.87611050245404 1103 84-85 4.07063976615331 1103 86-87 4.3035748740882624 1103 88-89 4.441647194302316 1103 90-91 4.654614854212529 1103 92-93 4.609156088312101 1103 94-95 4.70798610518483 1103 96-97 5.000427697572764 1103 98-99 4.936340007388974 1103 100-101 5.109041381900516 1104 1 0.5375939770272247 1104 2 0.5496119555760686 1104 3 0.5125071967461352 1104 4 0.4635328701050341 1104 5 0.36870808663089605 1104 6 0.3416312044615424 1104 7 0.3697083904022236 1104 8 0.43260159699400447 1104 9 0.5994662733367662 1104 10-11 0.6474796826803981 1104 12-13 0.6725986126781862 1104 14-15 0.8649452889009339 1104 16-17 0.9062684506921741 1104 18-19 0.9364028240954454 1104 20-21 0.8374011005012179 1104 22-23 0.9006519659308054 1104 24-25 0.9171591803090919 1104 26-27 0.9558866609488135 1104 28-29 0.9353491899848478 1104 30-31 1.0545868189510514 1104 32-33 1.1508531781896707 1104 34-35 1.1370418418071253 1104 36-37 1.4391972215212263 1104 38-39 1.269692123894366 1104 40-41 1.4716710131329407 1104 42-43 1.5225447598468804 1104 44-45 1.55982367857834 1104 46-47 1.5848310757911221 1104 48-49 1.7521646147840286 1104 50-51 1.7122115513585427 1104 52-53 1.6725639888643045 1104 54-55 1.8322490958246291 1104 56-57 2.0140634155849 1104 58-59 2.2582115723449263 1104 60-61 2.3306535773309527 1104 62-63 2.518672032759593 1104 64-65 2.6039911579271724 1104 66-67 2.661321377554252 1104 68-69 2.7663983922892363 1104 70-71 2.8038420374472715 1104 72-73 2.8640984430564096 1104 74-75 2.8564798958319066 1104 76-77 2.97174775171057 1104 78-79 3.1563607816225208 1104 80-81 3.500859562375638 1104 82-83 3.8326595813601614 1104 84-85 4.046559784347757 1104 86-87 4.037635575516976 1104 88-89 4.44947674839494 1104 90-91 4.71632373896259 1104 92-93 4.780513971855349 1104 94-95 5.197149549918169 1104 96-97 5.369918806266142 1104 98-99 5.6443326558488955 1104 100-101 5.473593353887621 1105 1 0.43286679878596246 1105 2 0.4298407699960727 1105 3 0.4393269427394415 1105 4 0.3398198193208444 1105 5 0.31646485326208307 1105 6 0.2836869994096318 1105 7 0.37432042281188416 1105 8 0.23075398781979573 1105 9 0.4796265014653187 1105 10-11 0.4897585809207641 1105 12-13 0.6027705136659662 1105 14-15 0.7536340245258373 1105 16-17 0.7603046879954647 1105 18-19 0.6668019171736361 1105 20-21 0.6992733216023126 1105 22-23 0.7049699346158889 1105 24-25 0.6468397660360239 1105 26-27 0.6442206603605527 1105 28-29 0.7261168156725262 1105 30-31 0.8214998027317151 1105 32-33 0.878715831503122 1105 34-35 0.9963621252795534 1105 36-37 1.1043487246821329 1105 38-39 1.1979252750783402 1105 40-41 1.2024593993634483 1105 42-43 1.4039140851556198 1105 44-45 1.289806637522858 1105 46-47 1.4844177774367964 1105 48-49 1.5531690742119295 1105 50-51 1.5095465122973266 1105 52-53 1.6206231946614338 1105 54-55 1.7011101281274534 1105 56-57 1.8011267220508742 1105 58-59 2.0242511360833575 1105 60-61 2.22960137129245 1105 62-63 2.337318798850003 1105 64-65 2.49550419789583 1105 66-67 2.637451369680022 1105 68-69 2.6623614295540143 1105 70-71 2.6997059543312396 1105 72-73 2.767639606042497 1105 74-75 2.951355774428894 1105 76-77 2.9518804134258367 1105 78-79 3.306920683174816 1105 80-81 3.5413788265502433 1105 82-83 3.838542900250804 1105 84-85 4.051669133318764 1105 86-87 4.298286433043465 1105 88-89 4.619487722201573 1105 90-91 4.8027546006833965 1105 92-93 4.833052521967527 1105 94-95 5.134867393828962 1105 96-97 5.499945159229664 1105 98-99 5.951048704645409 1105 100-101 5.791569095443924 1106 1 0.16697831218205295 1106 2 0.18417794332238557 1106 3 0.13394849497431593 1106 4 0.11766169938140081 1106 5 0.1895320641106153 1106 6 0.062482338755295075 1106 7 0.0833702549493367 1106 8 0.12660126581334197 1106 9 0.10132750864062245 1106 10-11 0.11803308556167025 1106 12-13 0.03575741380419828 1106 14-15 0.35081248545665034 1106 16-17 0.11887252421427519 1106 18-19 0.30098702677801015 1106 20-21 0.2368867769377161 1106 22-23 0.37296493165360545 1106 24-25 0.39665412980404824 1106 26-27 0.44362767767343314 1106 28-29 0.3034561613377207 1106 30-31 0.45624603405288866 1106 32-33 0.6161212689331776 1106 34-35 0.5393458656521375 1106 36-37 0.608742510476354 1106 38-39 0.5530161841692447 1106 40-41 0.6907980209156861 1106 42-43 0.22961877872418057 1106 44-45 0.7503277355414184 1106 46-47 1.0100779709724463 1106 48-49 1.0105361994834752 1106 50-51 0.29648311949896566 1106 52-53 0.763776772437744 1106 54-55 0.8148260125326985 1106 56-57 0.7832258596981632 1106 58-59 0.1418694627241308 1106 60-61 1.3162892786059963 1106 62-63 1.526608114892401 1106 64-65 1.645922603530753 1106 66-67 1.7686765688110881 1106 68-69 1.1966176338855306 1106 70-71 1.6512833356754548 1106 72-73 2.0440322069246015 1106 74-75 2.241821839862375 1106 76-77 2.2425313251498835 1106 78-79 2.6616384766051375 1106 80-81 3.1137487824451853 1106 82-83 3.2385900334217617 1106 84-85 3.335619893637375 1106 86-87 3.531466342200204 1106 88-89 3.5324040542402777 1106 90-91 4.117812727205678 1106 92-93 4.4292531670863475 1106 94-95 4.266911762204973 1106 96-97 4.550446377056009 1106 98-99 4.656808562455954 1106 100-101 4.150186829628639 1107 1 0.31511183095813067 1107 2 0.2604879698744149 1107 3 0.2308818620245141 1107 4 0.1810510027067309 1107 5 0.15186788406256824 1107 6 0.20161876561078174 1107 7 0.18623736836443072 1107 8 0.0986225074368079 1107 9 0.02571870853947189 1107 10-11 0.2507386109125349 1107 12-13 0.22575981613066887 1107 14-15 0.4111314891233562 1107 16-17 0.4758524205349275 1107 18-19 0.556462560979547 1107 20-21 0.5264981058035616 1107 22-23 0.4362393026233917 1107 24-25 0.5598612356328445 1107 26-27 0.5112205465569843 1107 28-29 0.47846716146415247 1107 30-31 0.6097490053514107 1107 32-33 0.6412446726504584 1107 34-35 0.6812716464082413 1107 36-37 0.6917834764644653 1107 38-39 0.7121811860658269 1107 40-41 0.8090844035177795 1107 42-43 0.9663149476456638 1107 44-45 1.086185745403597 1107 46-47 0.8106095173120593 1107 48-49 0.9406113037950163 1107 50-51 1.0551067128736022 1107 52-53 1.1763821587065522 1107 54-55 1.3045254688482828 1107 56-57 1.4035815304529962 1107 58-59 1.3662960653287612 1107 60-61 1.4150250226950085 1107 62-63 1.6445394589308329 1107 64-65 1.8049858226482094 1107 66-67 1.8551118956539199 1107 68-69 2.015910338399376 1107 70-71 2.020825882141505 1107 72-73 2.307884147751505 1107 74-75 2.4704183754547344 1107 76-77 2.703095618992343 1107 78-79 2.682528348902519 1107 80-81 2.978560642351617 1107 82-83 3.1941648216377168 1107 84-85 3.428333384597039 1107 86-87 3.5879856240310275 1107 88-89 3.840823196989046 1107 90-91 4.101991890184561 1107 92-93 4.389992072131232 1107 94-95 4.46765281669985 1107 96-97 4.6604652163530105 1107 98-99 4.990434558157052 1107 100-101 4.865582201989239 1108 1 0.3367735877726048 1108 2 0.36473951937043836 1108 3 0.29433453960411526 1108 4 0.4167237889522255 1108 5 0.3008412670610383 1108 6 0.2945205572981351 1108 7 0.315093014900782 1108 8 0.3408412209968077 1108 9 0.4053435678980364 1108 10-11 0.432713985627025 1108 12-13 0.505899519558767 1108 14-15 0.39217727335618946 1108 16-17 0.5805492849749143 1108 18-19 0.6501141625658704 1108 20-21 0.6176522059626421 1108 22-23 0.5591918775936691 1108 24-25 0.6429075921923939 1108 26-27 0.633815595883263 1108 28-29 0.7457411197890593 1108 30-31 0.7867716946298984 1108 32-33 0.8633664215894896 1108 34-35 1.0137037754588647 1108 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2.2325967207819417 2311 80-81 2.090031474870443 2311 82-83 2.018113662449089 2311 84-85 2.36479137985123 2311 86-87 2.6922035189441047 2311 88-89 2.7250632215692505 2311 90-91 2.918623022492099 2311 92-93 3.1498823778218146 2311 94-95 3.0849944179486926 2311 96-97 3.410553487161774 2311 98-99 3.6346959501187293 2311 100-101 3.6291576678018966 2312 1 -0.28660963410029083 2312 2 -0.41092576986583396 2312 3 -0.2114509035308778 2312 4 -0.2782870842293548 2312 5 -0.2593358750252577 2312 6 -0.2220169614858989 2312 7 -0.2970718735085143 2312 8 -0.2683666643894398 2312 9 -0.25487592762177513 2312 10-11 -0.2522758207302047 2312 12-13 -0.23699820659897597 2312 14-15 -0.2661706310010814 2312 16-17 -0.11759985764559389 2312 18-19 -0.1385844230626887 2312 20-21 -0.1727287428193094 2312 22-23 -0.35158635917806436 2312 24-25 -0.3509887252118844 2312 26-27 -0.09765797280448396 2312 28-29 -0.21285881945613738 2312 30-31 -0.06369977186081854 2312 32-33 -0.03165105635044796 2312 34-35 -0.2544086530031322 2312 36-37 -0.09942971952097679 2312 38-39 0.08967109885822566 2312 40-41 -0.032254594991179886 2312 42-43 0.144601359196443 2312 44-45 -0.03880480175423884 2312 46-47 -0.0842733972793539 2312 48-49 0.23236325483886588 2312 50-51 0.12862283304450983 2312 52-53 0.09730867846163704 2312 54-55 0.36101741889900296 2312 56-57 0.4013605111587282 2312 58-59 0.489474798757243 2312 60-61 0.5230832517471704 2312 62-63 0.5265240842816716 2312 64-65 0.6257359614797124 2312 66-67 0.7042854780806529 2312 68-69 0.7913048863644612 2312 70-71 0.8881053587228287 2312 72-73 0.9653364382321072 2312 74-75 1.0424691912008477 2312 76-77 1.1825035134594444 2312 78-79 1.3311205359804141 2312 80-81 1.4153809773056807 2312 82-83 1.565485843802012 2312 84-85 1.8362363364804786 2312 86-87 2.138301109790948 2312 88-89 2.1067599485915878 2312 90-91 2.2721490283104053 2312 92-93 2.447077899838039 2312 94-95 2.630991266716183 2312 96-97 2.992640880227256 2312 98-99 3.1914727080057865 2312 100-101 3.0012169341458073 2313 1 -0.3610480730422232 2313 2 -0.4176567892273866 2313 3 -0.37571545321469557 2313 4 -0.34733926008129856 2313 5 -0.2724314763067497 2313 6 -0.24994150389282055 2313 7 -0.17728193982159013 2313 8 -0.22094466846345995 2313 9 -0.2163661828921235 2313 10-11 -0.2029310545198868 2313 12-13 -0.2743945411010529 2313 14-15 -0.1972786634999366 2313 16-17 -0.1506072071361686 2313 18-19 -0.03453504086411385 2313 20-21 -0.12545707050772137 2313 22-23 -0.161517678084941 2313 24-25 -0.0299457742496827 2313 26-27 -0.05904754420231484 2313 28-29 -0.06844849205638326 2313 30-31 -0.1465651916607129 2313 32-33 -0.021090052726698616 2313 34-35 0.08100297766940656 2313 36-37 0.0602802893201968 2313 38-39 -0.04729240302192039 2313 40-41 -0.004319033533825234 2313 42-43 0.21807044579062307 2313 44-45 0.2026552721209356 2313 46-47 0.1522197315864986 2313 48-49 0.323752558322532 2313 50-51 0.2772072739724578 2313 52-53 0.272873318372028 2313 54-55 0.35845829845258237 2313 56-57 0.46755675439713684 2313 58-59 0.4724259510906492 2313 60-61 0.6533103867854102 2313 62-63 0.7388602137170572 2313 64-65 0.7099208045381822 2313 66-67 0.8695393656448545 2313 68-69 1.0298242781647353 2313 70-71 1.2205336355075467 2313 72-73 1.3593968027509398 2313 74-75 1.4380517511120772 2313 76-77 1.726690715891067 2313 78-79 1.737688008867071 2313 80-81 1.8509464317615674 2313 82-83 2.096486051651553 2313 84-85 2.3556093395476623 2313 86-87 2.385355404325413 2313 88-89 2.35985776016496 2313 90-91 2.6193042119742067 2313 92-93 2.6290898185615212 2313 94-95 2.892619605812552 2313 96-97 3.1400299702438232 2313 98-99 3.1793992316811845 2313 100-101 3.040176515404605 2314 1 -0.4127902611249823 2314 2 -0.4647358095913674 2314 3 -0.6213402504682755 2314 4 -0.4848089240122988 2314 5 -0.3953955552785402 2314 6 -0.2931377676340361 2314 7 -0.3470353394562622 2314 8 -0.3328665484237021 2314 9 -0.44174582827886866 2314 10-11 -0.4078791731198166 2314 12-13 -0.5811521552592112 2314 14-15 -0.5901478875037256 2314 16-17 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1.8357885887926813 2314 82-83 2.048813211952634 2314 84-85 2.390040669442765 2314 86-87 2.418587494552124 2314 88-89 2.65982369442915 2314 90-91 2.6525585284130173 2314 92-93 2.7267491924285245 2314 94-95 2.7457548678173076 2314 96-97 2.9372688024938185 2314 98-99 3.1068373712539916 2314 100-101 3.1718656294612853 2315 1 -0.46796329124915914 2315 2 -0.4592201551149735 2315 3 -0.48488105422936556 2315 4 -0.37874200776595046 2315 5 -0.3440561306045993 2315 6 -0.2549100609927635 2315 7 -0.3970315326395166 2315 8 -0.33943802514973953 2315 9 -0.48942441841615647 2315 10-11 -0.40179439411800644 2315 12-13 -0.34251870268900575 2315 14-15 -0.5105050515098455 2315 16-17 -0.31606056117608006 2315 18-19 -0.40355260910573776 2315 20-21 -0.39222710686900797 2315 22-23 -0.476111640734743 2315 24-25 -0.37140711610938837 2315 26-27 -0.5553103265855839 2315 28-29 -0.344549657323995 2315 30-31 -0.43218502680311843 2315 32-33 -0.3562498657058555 2315 34-35 -0.389311523148443 2315 36-37 -0.25441084425206384 2315 38-39 -0.17686852234825068 2315 40-41 -0.36271423126631674 2315 42-43 -0.2706445607077512 2315 44-45 -0.32130856810874775 2315 46-47 -0.3623359938156625 2315 48-49 -0.24646610998534157 2315 50-51 -0.1352586998825771 2315 52-53 0.020426363031909034 2315 54-55 0.09039789100064155 2315 56-57 0.27448982898523155 2315 58-59 0.42620436569644937 2315 60-61 0.5288046200774552 2315 62-63 0.5714749755144553 2315 64-65 0.7812318024150038 2315 66-67 0.9131760739249053 2315 68-69 1.1859914978968078 2315 70-71 1.1764636841952942 2315 72-73 1.2115682736292577 2315 74-75 1.2214049357503214 2315 76-77 1.379255538580793 2315 78-79 1.5064701253259294 2315 80-81 1.551395463708495 2315 82-83 1.941290432330014 2315 84-85 2.0795044201962654 2315 86-87 2.348384371053566 2315 88-89 2.603407863364179 2315 90-91 2.7330237886615443 2315 92-93 2.9282788711744097 2315 94-95 2.819616060216429 2315 96-97 2.7846620874426193 2315 98-99 2.928879046244681 2315 100-101 2.946854236125146 2316 1 -0.7208427476940642 2316 2 -0.6400583076943214 2316 3 -0.7821153228321016 2316 4 -0.5978202501900185 2316 5 -0.5855371969792955 2316 6 -0.49960487891347327 2316 7 -0.6148896461956284 2316 8 -0.6013038834077022 2316 9 -0.4054847293622572 2316 10-11 -0.614356578456345 2316 12-13 -0.6426072236230667 2316 14-15 -1.1098860066912621 2316 16-17 -0.9089102449779816 2316 18-19 -0.7570138491526777 2316 20-21 -0.6304734182696947 2316 22-23 -0.8355788961634474 2316 24-25 -0.8350508066909796 2316 26-27 -0.5899264736123015 2316 28-29 -0.6251214523768596 2316 30-31 -0.4813651778597645 2316 32-33 -0.5200726871451593 2316 34-35 -0.45630939306024487 2316 36-37 -0.4732476040203082 2316 38-39 -0.46312083641512203 2316 40-41 -0.5913033367768676 2316 42-43 -0.35064712880257076 2316 44-45 -0.24037100687239388 2316 46-47 -0.19221208721437932 2316 48-49 0.19593743829971544 2316 50-51 0.18714181936757512 2316 52-53 0.2059404463412733 2316 54-55 0.17525872228680583 2316 56-57 0.3316595705159102 2316 58-59 0.5161971859300394 2316 60-61 0.5380318801201049 2316 62-63 0.7249487267925439 2316 64-65 0.7650130120898417 2316 66-67 0.9470069198090165 2316 68-69 1.0835745255829572 2316 70-71 0.8994466341801122 2316 72-73 1.1680333331211834 2316 74-75 1.2660907505800125 2316 76-77 1.4481019939855209 2316 78-79 1.53121965660889 2316 80-81 1.8806050672477852 2316 82-83 2.1984687795895645 2316 84-85 2.0370872026532005 2316 86-87 2.282650850740527 2316 88-89 2.321745479504621 2316 90-91 2.646038091655406 2316 92-93 2.7308084445686696 2316 94-95 2.7078322402128556 2316 96-97 2.7165521144019387 2316 98-99 2.9704653227637827 2316 100-101 2.824127516608554 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7961.0 3 2690.0 4 2732.0 5 3204.0 6 3793.0 7 4626.0 8 5968.0 9 7586.0 10 9894.0 11 12242.0 12 15563.0 13 19084.0 14 22249.0 15 27013.0 16 32642.0 17 38618.0 18 36571.0 19 32269.0 20 34370.0 21 37593.0 22 41288.0 23 46415.0 24 52870.0 25 59331.0 26 67010.0 27 76069.0 28 86352.0 29 98155.0 30 111708.0 31 128167.0 32 149777.0 33 175911.0 34 211801.0 35 257540.0 36 304625.0 37 323714.0 38 187560.0 39 15581.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.013747549248436 18.442926664177016 14.472636191765476 23.070689594809075 2 15.625013638327555 20.16668582094003 40.10377494359188 24.104525597140533 3 19.21101819040182 24.571645552493493 29.35258638467719 26.864749872427502 4 12.903672833096124 16.498851182589043 36.25807418410685 34.33940180020797 5 14.207717063556588 37.114855261860775 33.190117566430544 15.487310108152094 6 32.366982462215844 35.37196311725273 17.37085290989278 14.890201510638637 7 29.29406330918507 32.655654476157224 19.894351342701697 18.15593087195601 8 26.858764282485254 34.52716368036328 19.450329119522085 19.163742917629378 9 27.386625426543574 16.619432565197243 19.6624313583999 36.331510649859275 10-11 25.577457894206635 26.484053968442154 26.549352452157176 21.389135685194027 12-13 26.577429513941738 23.88940441101461 27.147613350768346 22.38555272427531 14-15 23.231387435083338 24.981439393870517 25.877930761984025 25.909242409062124 16-17 22.716320541678638 28.50767462917777 26.19886762401044 22.57713720513315 18-19 22.616444025191083 26.45625489570022 29.180409780563533 21.74689129854516 20-21 23.40264266647415 25.591304852259483 29.11941243851002 21.886640042756344 22-23 22.99182167111684 25.572279363684697 28.98829911270514 22.447599852493322 24-25 22.797576718749145 25.762076848788634 29.133700596362893 22.306645836099324 26-27 22.357978254342395 26.25609842813452 29.015905969953554 22.370017347569533 28-29 22.30604547586285 26.369028449373054 29.19347163018488 22.131454444579212 30-31 22.259920991397212 26.011556699328697 29.09552534319862 22.63299696607547 32-33 22.125976749311373 26.12267527663199 28.930599974833456 22.820747999223183 34-35 22.98339965567178 26.164053413788114 28.546602504562312 22.3059444259778 36-37 22.815140826502663 25.83203593369679 28.619435864817323 22.733387374983224 38-39 22.40729724009882 26.204606792808505 28.786655107988068 22.601440859104606 40-41 22.68350229751162 25.946890600338428 28.80406670374491 22.565540398405044 42-43 22.63567892873214 25.89413684483252 28.574561024722616 22.89562320171272 44-45 22.49470007448347 26.32157262847763 28.37114986158654 22.812577435452354 46-47 22.58758401409133 26.55875263789747 27.881142208014044 22.972521139997156 48-49 22.757753265271948 26.503832293589124 27.86893859548808 22.869475845650847 50-51 22.80095006003776 26.70657670244087 27.571411300915067 22.921061936606307 52-53 23.082076156673427 26.60827898687068 27.500437207702568 22.80920764875333 54-55 22.54258853891229 26.563446183470262 27.702824922641906 23.19114035497554 56-57 22.633940712537395 26.986659375595366 27.577083445438404 22.802316466428834 58-59 22.80626357214861 26.32689142008419 27.681720218990506 23.18512478877669 60-61 22.745358680893325 26.436835102394163 27.98280526320669 22.835000953505823 62-63 22.626319918048505 26.60842075219763 27.838529381555276 22.926729948198584 64-65 22.37578241037845 26.353599496784913 27.831321139578556 23.439296953258083 66-67 22.428829072521513 26.68482354038314 27.61331821593627 23.273029171159074 68-69 22.500724909715792 26.466921847006326 27.83411520565166 23.198238037626222 70-71 22.4755633998963 26.658747179273483 27.537653926400402 23.328035494429816 72-73 22.735718464666093 26.512657185337524 27.44710613171443 23.30451821828195 74-75 22.503505128249877 26.791833196618786 27.327710584032904 23.376951091098427 76-77 22.684868888228756 26.577717161881655 27.401285756722842 23.33612819316675 78-79 22.73579225455812 26.657797544035954 27.341415651589646 23.26499454981628 80-81 22.596437990429873 26.948352915532485 27.213593862117246 23.24161523192039 82-83 22.807740618978265 26.806495615672134 26.997082205306643 23.388681560042958 84-85 22.740952438778976 26.46945097727677 27.143292660203056 23.6463039237412 86-87 22.84927315350742 26.442095144467647 27.241582658065116 23.467049043959815 88-89 22.76858562742146 26.430756995899852 27.209659997203655 23.590997379475034 90-91 22.695688382343317 26.54741470226709 27.216977358188654 23.53991955720094 92-93 22.973719112389404 26.162259550922347 27.214967679535164 23.64905365715309 94-95 22.78545547637853 26.089490159115275 27.21434452042142 23.910709844084778 96-97 22.995829343249284 26.018232782609314 27.151795433277222 23.83414244086418 98-99 22.853141579518237 26.015592360245265 26.854097148818283 24.27716891141822 100-101 23.316677575796614 25.569053518790362 27.17155830597862 23.9427105994344 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1383.0 1 933.5 2 717.5 3 970.0 4 834.5 5 783.0 6 1315.5 7 2831.0 8 4606.5 9 5238.0 10 4442.5 11 3554.0 12 3500.0 13 3723.0 14 3925.0 15 4087.0 16 4219.0 17 4394.0 18 4610.5 19 4812.0 20 5147.5 21 5820.0 22 6830.0 23 7973.0 24 9304.0 25 11139.0 26 13482.0 27 16104.0 28 18858.5 29 21912.0 30 25829.0 31 30388.0 32 35408.0 33 39766.5 34 43446.5 35 47642.0 36 52604.5 37 58049.0 38 62183.0 39 64670.5 40 68305.5 41 75404.5 42 83317.5 43 90129.0 44 94797.5 45 97543.0 46 98415.5 47 101428.0 48 109696.5 49 113877.5 50 113110.0 51 110913.5 52 106421.0 53 104247.5 54 102420.0 55 94545.5 56 83871.0 57 75818.5 58 69070.0 59 60596.0 60 51199.5 61 42804.5 62 35575.5 63 27915.0 64 21773.5 65 17649.5 66 13752.0 67 10983.5 68 8459.0 69 6192.5 70 4988.5 71 4058.5 72 3423.0 73 2970.5 74 2347.0 75 1853.5 76 1416.0 77 932.0 78 559.0 79 368.5 80 238.0 81 137.5 82 86.0 83 59.0 84 33.5 85 23.5 86 14.5 87 9.0 88 7.5 89 9.0 90 11.0 91 9.0 92 5.5 93 2.0 94 0.5 95 0.0 96 0.5 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31001151409012695 2 0.03413871370125826 3 0.04093737127541726 4 0.043409610393293246 5 0.05613437055883147 6 0.054789181627046005 7 0.03137562292245567 8 0.033956931413179146 9 0.040610163156874844 10-11 0.03133926646483985 12-13 0.03284805945589653 14-15 0.02790358122014453 16-17 0.020832250213866862 18-19 0.024758747636375797 20-21 0.036701843963173816 22-23 0.030430355024444265 24-25 0.05009919859460478 26-27 0.042409807808858106 28-29 0.035429367946619994 30-31 0.03522940742973296 32-33 0.03130291000722403 34-35 0.030575780854907558 36-37 0.02952144358404868 38-39 0.018796288587380746 40-41 0.018269119951951306 42-43 0.019287100765194364 44-45 0.009761708869848607 46-47 0.01757834725725066 48-49 0.015542385630764544 50-51 0.006925905175814376 52-53 0.004871765320520348 54-55 0.006707766430119434 56-57 0.005344399269526054 58-59 0.007107687463893494 60-61 0.006053350193034612 62-63 0.004926300006944083 64-65 0.008289272336407756 66-67 0.002526773804299733 68-69 0.007289469751972611 70-71 0.013270107029775576 72-73 0.013306463487391397 74-75 0.010434303335741343 76-77 0.009307253149650814 78-79 0.006435092998000758 80-81 0.0077621037009783156 82-83 0.012706581936730311 84-85 0.004344596685090908 86-87 0.0011270501860905283 88-89 0.018959892646651953 90-91 0.027739977160873324 92-93 0.012742938394346134 94-95 0.02134124062048839 96-97 0.018050981206256367 98-99 0.012724760165538224 100-101 0.10341594368820993 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2750543.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.61358892601122 #Duplication Level Percentage of deduplicated Percentage of total 1 71.7482670107706 32.00947690572211 2 14.283841689743326 12.745068828608602 3 5.471123414285759 7.322593529052594 4 2.6941487940348683 4.80782587210322 5 1.5246065415158006 3.400908475854679 6 0.9207350499798906 2.4646377017743943 7 0.6154063169810612 1.9218839113784536 8 0.4437973452591831 1.5839513862278616 9 0.33022524523781793 1.3259280009628127 >10 1.7095101583741095 13.974728396625204 >50 0.14641748589340803 4.540380414652816 >100 0.10178157921519934 8.869060780634607 >500 0.00717174859904339 2.181447786331587 >1k 0.002720318434115431 1.7021368247126993 >5k 8.243389194289185E-5 0.26479236734722117 >10k+ 1.648677838857837E-4 0.8851788180111481 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13644 0.49604750771029577 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10425 0.3790160706449599 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7200 0.26176649483392916 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 7.271291523164698E-5 0.0 0.0 0.0 0.0 8 1.0906937284747048E-4 0.0 0.0 0.0 0.0 9 1.0906937284747048E-4 0.0 0.0 0.0 0.0 10-11 1.0906937284747048E-4 0.0 0.0 0.0 0.0 12-13 1.2724760165538224E-4 0.0 0.0 0.0 0.0 14-15 2.1813874569494095E-4 0.0 0.0 0.0 0.0 16-17 2.363169745028527E-4 0.0 0.0 0.0 0.0 18-19 3.2720811854241146E-4 0.0 0.0 0.0 0.0 20-21 3.9992103377405844E-4 0.0 0.0 3.635645761582349E-5 0.0 22-23 3.9992103377405844E-4 0.0 0.0 1.4542583046329397E-4 0.0 24-25 5.817033218531759E-4 0.0 0.0 4.5445572019779364E-4 0.0 26-27 6.544162370848229E-4 0.0 0.0 9.99802584435146E-4 0.0 28-29 7.089509235085581E-4 0.0 0.0 0.004180992625819701 0.0 30-31 7.998420675481169E-4 0.0 0.0 0.013651849834741723 0.0 32-33 8.180202963560286E-4 0.0 0.0 0.033829683811523764 0.0 34-35 9.270896692034991E-4 0.0 0.0 0.06409643477669683 0.0 36-37 9.816243556272344E-4 0.0 0.0 0.11505001012527344 0.0 38-39 0.0010361590420509695 0.0 0.0 0.19196209621154806 0.0 40-41 0.0010725154996667932 0.0 0.0 0.27563648341436586 0.0 42-43 0.0011997631013221754 0.0 0.0 0.3618194661926754 0.0 44-45 0.0013451889317854693 0.0 0.0 0.46901648147293096 0.0 46-47 0.0014179018470171162 0.0 0.0 0.5954097063743413 0.0 48-49 0.0014906147622487632 0.0 0.0 0.7326916903316909 0.0 50-51 0.0014906147622487632 0.0 0.0 0.8820803746751096 0.0 52-53 0.001508792991056675 0.0 0.0 1.0277788785705222 0.0 54-55 0.0015996841350962338 0.0 0.0 1.1832027348781677 0.0 56-57 0.0017451099655595276 0.0 0.0 1.364785062440398 0.0 58-59 0.0017451099655595276 0.0 0.0 1.567308709589343 0.0 60-61 0.0017451099655595276 0.0 0.0 1.79200979588394 0.0 62-63 0.0017451099655595276 0.0 0.0 2.0152566238739045 0.0 64-65 0.0017451099655595276 0.0 0.0 2.2428116920913435 0.0 66-67 0.0018905357960228217 0.0 0.0 2.501615135629583 0.0 68-69 0.0018905357960228217 0.0 0.0 2.79177602386147 7.271291523164698E-5 70-71 0.0019268922536386452 0.0 0.0 3.102841875222456 7.271291523164698E-5 72-73 0.002017783397678204 0.0 0.0 3.41967022511555 7.271291523164698E-5 74-75 0.002072318084101939 0.0 0.0 3.744660599743396 1.0906937284747048E-4 76-77 0.002072318084101939 0.0 0.0 4.092046552262589 1.0906937284747048E-4 78-79 0.002072318084101939 0.0 0.0 4.4769705472701204 1.0906937284747048E-4 80-81 0.002308635058604792 0.0 0.0 4.896869454504074 1.0906937284747048E-4 82-83 0.0023268132874127035 0.0 0.0 5.325203059904899 1.0906937284747048E-4 84-85 0.002381347973836439 0.0 0.0 5.751191673789503 1.0906937284747048E-4 86-87 0.0024540608890680857 1.8178228807911746E-5 0.0 6.191886474779707 1.0906937284747048E-4 88-89 0.0024722391178759974 3.635645761582349E-5 0.0 6.6666836330135535 1.0906937284747048E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6675 0.0 49.114796 1 GTATCAA 11960 0.0 42.153114 1 ATCAACG 15580 0.0 32.058216 3 TATCAAC 15775 0.0 31.692059 2 TCAACGC 15990 0.0 31.20649 4 CAACGCA 16150 0.0 30.838472 5 AACGCAG 16645 0.0 29.919199 6 GTACATG 14260 0.0 26.93337 1 ACGCAGA 18845 0.0 26.269823 7 TACATGG 14570 0.0 26.061014 2 CGCAGAG 18995 0.0 25.962328 8 ACATGGG 14965 0.0 24.261671 3 GCAGAGT 20005 0.0 23.86697 9 GAGTACT 10185 0.0 22.73694 12-13 AGTACTT 10540 0.0 21.993666 12-13 AGAGTAC 17945 0.0 21.376144 10-11 GTACTTT 10940 0.0 21.124956 14-15 TGGTATC 3275 0.0 21.04073 2 CATGGGG 7095 0.0 20.898075 4 CATGGGA 8490 0.0 20.598907 4 >>END_MODULE