FastQCFastQC Report
Thu 26 May 2016
SRR938325_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938325_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2750543
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125450.45609176079050573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103960.37796173337410105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77060.28016286238753585No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG28160.10237978464615895No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67900.049.2421041
GTATCAA120850.040.2605821
ATCAACG156350.030.9237633
TATCAAC159050.030.4713422
TCAACGC160650.030.096054
CAACGCA163050.029.6822095
AACGCAG168200.028.745676
GTACATG137450.025.4327721
ACGCAGA190500.025.23117
CGCAGAG193350.024.7854968
TACATGG139550.024.300252
GCAGAGT204300.023.2943179
GAGTACT107850.023.27423912-13
ACATGGG141850.023.0583553
TGGTATC33850.022.6213382
GTGGTAT38150.021.8169771
GGACCGA8300.021.7448356
ATTCGCG3550.021.40750180-81
GTACTTT116600.021.34437214-15
AGAGTAC184350.020.77838910-11
Produced by FastQC (version 0.11.5)