FastQCFastQC Report
Thu 26 May 2016
SRR938324_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938324_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741066
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127810.46627844787392936No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98560.35956813881898503No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70370.256724938399878No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG27870.10167577139696744No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66800.050.1791461
GTATCAA118750.041.758431
ATCAACG154750.031.8141373
TATCAAC156450.031.4678672
TCAACGC157850.031.2194464
CAACGCA159800.030.897965
AACGCAG165900.029.757526
GTACATG144150.026.9249041
TACATGG145100.026.0365122
ACGCAGA189600.025.9579127
CGCAGAG192700.025.5403238
ACATGGG150900.024.0599753
GCAGAGT202000.023.5156699
GAGTACT102550.023.32150812-13
AGTACTT107150.021.61102112-13
GTACTTT111100.021.50634414-15
CATGGGG68550.021.2824654
AGAGTAC181100.021.06133810-11
TGGTATC32550.021.0229972
CAGAGTA197750.020.17677310-11
Produced by FastQC (version 0.11.5)