FastQCFastQC Report
Thu 26 May 2016
SRR938324_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938324_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2741066
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121780.44427970723798693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99530.3631069080423456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72690.2651887988103898No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG28960.10565232650363034No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA66750.052.459751
GTATCAA116550.040.5213741
ATCAACG154500.030.4356143
TATCAAC156600.030.059452
TCAACGC159000.029.6041034
CAACGCA161200.029.1706095
AACGCAG165300.028.4758136
ACGCAGA191100.024.5816447
GTACATG141300.024.5465661
CGCAGAG192750.024.395868
TACATGG141950.023.6582782
TGCACCG6750.023.2212455
ACATGGG147350.022.4031963
GCAGAGT207900.022.2068589
GCACCGT7150.021.9221536
GAGTACT109250.021.91212312-13
TAAGGTG11150.021.7255445
GTAAGGT9050.021.5184334
AGTACTT112150.021.49374612-13
ATTCGCG3250.021.19218880-81
Produced by FastQC (version 0.11.5)