##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938324_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2741066 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.83177238344498 31.0 31.0 34.0 30.0 34.0 2 32.0867443542038 33.0 31.0 34.0 30.0 34.0 3 32.13553595571942 33.0 31.0 34.0 30.0 34.0 4 35.78654983134299 37.0 35.0 37.0 35.0 37.0 5 35.68323783520718 37.0 35.0 37.0 33.0 37.0 6 35.718983782221954 37.0 35.0 37.0 33.0 37.0 7 35.63780478105963 37.0 35.0 37.0 33.0 37.0 8 35.61542188331109 37.0 35.0 37.0 33.0 37.0 9 37.158692640016696 39.0 37.0 39.0 33.0 39.0 10-11 37.088091275438096 39.0 37.0 39.0 33.0 39.0 12-13 36.96734919918018 39.0 37.0 39.0 33.0 39.0 14-15 37.96884423797165 40.0 37.0 41.0 33.0 41.0 16-17 38.06552195386759 40.0 37.0 41.0 33.0 41.0 18-19 38.21675326314653 40.0 37.5 41.0 33.0 41.0 20-21 38.17526648391538 40.0 37.5 41.0 33.0 41.0 22-23 38.04548139300549 40.0 37.0 41.0 33.0 41.0 24-25 37.92372566001694 40.0 37.0 41.0 33.0 41.0 26-27 37.71209120831092 40.0 37.0 41.0 32.0 41.0 28-29 37.563827722499205 39.5 36.5 41.0 32.0 41.0 30-31 37.29399638680718 39.0 36.0 41.0 31.0 41.0 32-33 37.185683234186996 39.0 36.0 41.0 31.0 41.0 34-35 37.04025751295299 39.0 36.0 41.0 31.0 41.0 36-37 36.899677351804 39.0 35.0 41.0 31.0 41.0 38-39 36.69461133004459 39.0 35.0 40.5 30.0 41.0 40-41 36.467420521796996 39.0 35.0 40.0 30.0 41.0 42-43 36.18648547681814 38.5 35.0 40.0 30.0 41.0 44-45 35.86105387466044 38.0 35.0 40.0 29.0 41.0 46-47 35.39122206470037 38.0 34.5 40.0 27.5 41.0 48-49 34.958138731427844 38.0 34.0 40.0 26.0 41.0 50-51 34.557091657041454 38.0 33.0 40.0 24.5 41.0 52-53 33.97001513279869 37.5 33.0 40.0 23.5 41.0 54-55 33.54562403823914 37.0 32.5 40.0 22.5 41.0 56-57 33.15915195037259 37.0 32.0 39.5 21.5 40.5 58-59 32.63317446570057 36.0 31.0 39.0 19.5 40.0 60-61 32.15285877830012 36.0 31.0 39.0 18.0 40.0 62-63 31.65472502303848 35.0 30.0 38.5 13.5 40.0 64-65 31.011863815026707 35.0 29.0 38.0 8.5 40.0 66-67 30.3630167971147 34.0 28.5 38.0 7.5 40.0 68-69 29.652358972750015 34.0 28.0 37.0 4.0 39.0 70-71 28.895704809734607 33.5 26.0 36.0 2.0 39.0 72-73 28.323318008395276 33.0 26.0 36.0 2.0 38.5 74-75 27.614403666310842 32.5 25.5 35.0 2.0 38.0 76-77 26.472684167400566 30.5 24.0 34.0 2.0 36.0 78-79 26.986639504484753 32.0 25.0 35.0 2.0 36.0 80-81 26.608990261453023 32.0 24.5 35.0 2.0 36.0 82-83 25.956297476966988 31.5 22.5 34.5 2.0 36.0 84-85 25.203091972247293 31.0 20.0 34.0 2.0 35.0 86-87 24.32386086289057 31.0 16.5 34.0 2.0 35.0 88-89 23.38266280344946 30.0 7.5 34.0 2.0 35.0 90-91 22.550400282225965 29.5 2.0 34.0 2.0 35.0 92-93 21.48714569441232 29.0 2.0 34.0 2.0 35.0 94-95 20.340388921682294 27.5 2.0 33.0 2.0 35.0 96-97 18.99026692534948 25.5 2.0 33.0 2.0 34.0 98-99 17.256461354815972 21.0 2.0 32.0 2.0 34.0 100-101 12.993129497793923 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34743226899718493 1101 2 0.31324711252257487 1101 3 0.25601290375433905 1101 4 0.2069364351332581 1101 5 0.19124262407579806 1101 6 0.23682276617997644 1101 7 0.2615891223856295 1101 8 0.2309514631804177 1101 9 0.2978013934015067 1101 10-11 0.29686735427387845 1101 12-13 0.30142653766650795 1101 14-15 0.5135838982971919 1101 16-17 0.3992912385285692 1101 18-19 0.432130973839044 1101 20-21 0.45641966193196026 1101 22-23 0.5576786860665877 1101 24-25 0.3985926639453865 1101 26-27 0.5182705589553507 1101 28-29 0.6421977551276896 1101 30-31 0.5136216060141763 1101 32-33 0.5412625845695302 1101 34-35 0.3958489037432855 1101 36-37 0.49488372428575644 1101 38-39 0.5020841169327426 1101 40-41 0.4946089679698318 1101 42-43 0.5722529429635657 1101 44-45 0.6817807720997209 1101 46-47 0.7440632550818407 1101 48-49 0.8207535662666743 1101 50-51 0.9030935097462347 1101 52-53 0.9109064221375789 1101 54-55 0.928737953218608 1101 56-57 1.0065734939280162 1101 58-59 1.0708526500250954 1101 60-61 1.2717721196786087 1101 62-63 1.4065143858354965 1101 64-65 1.41045095544332 1101 66-67 1.6055908646458334 1101 68-69 1.4502226834393994 1101 70-71 1.6652634921129064 1101 72-73 1.8474385881438131 1101 74-75 1.969122617949786 1101 76-77 1.9035773627898713 1101 78-79 1.848071956592083 1101 80-81 1.9357167494443672 1101 82-83 2.068810997812001 1101 84-85 2.2203196627707023 1101 86-87 2.2933221769581458 1101 88-89 2.262792034665818 1101 90-91 2.4903268785169814 1101 92-93 2.6868701876322945 1101 94-95 2.674937683121076 1101 96-97 2.633788368802172 1101 98-99 2.7298320157993814 1101 100-101 2.488372051778864 1102 1 0.24868699714190612 1102 2 0.24957491267220178 1102 3 0.2966798665044621 1102 4 0.25514234825759985 1102 5 0.22544205118412464 1102 6 0.25884392323038696 1102 7 0.2679419008506301 1102 8 0.28209068897186285 1102 9 0.3786029648296534 1102 10-11 0.40529820636826486 1102 12-13 0.4099132779963526 1102 14-15 0.561829380292231 1102 16-17 0.4362190547740141 1102 18-19 0.3733889360496576 1102 20-21 0.3845518621996291 1102 22-23 0.46912593800480096 1102 24-25 0.37280933443729936 1102 26-27 0.3431166054960926 1102 28-29 0.46340368911697993 1102 30-31 0.3906269504198434 1102 32-33 0.3989708390383555 1102 34-35 0.6359566860682904 1102 36-37 0.5635539717737643 1102 38-39 0.6288691871730805 1102 40-41 0.6941092526272428 1102 42-43 0.5636352751274671 1102 44-45 0.6297212533644938 1102 46-47 0.5624828252044267 1102 48-49 0.6767162448482154 1102 50-51 0.7502227919028357 1102 52-53 0.7014087754048788 1102 54-55 0.6872666469838649 1102 56-57 0.7339924857936708 1102 58-59 1.0287849790592531 1102 60-61 0.9453514407334254 1102 62-63 1.076939598434354 1102 64-65 1.096200871238377 1102 66-67 1.183171783359004 1102 68-69 1.4768436658204465 1102 70-71 1.5344169865434587 1102 72-73 1.6024967715228016 1102 74-75 1.5226853811833756 1102 76-77 1.5221401558305168 1102 78-79 1.6220572497892185 1102 80-81 1.6968584045111186 1102 82-83 1.8403177595433178 1102 84-85 1.8128950224673872 1102 86-87 1.7437912655477028 1102 88-89 1.5538749549229927 1102 90-91 1.9112744598231899 1102 92-93 2.0846914406614374 1102 94-95 1.735477682703774 1102 96-97 2.0549489215442733 1102 98-99 2.194887859324247 1102 100-101 2.056501673735937 1103 1 0.1494078202219491 1103 2 0.10994734268813033 1103 3 0.16666450797705323 1103 4 0.1443237216182638 1103 5 0.08636630338370566 1103 6 0.10113182996015979 1103 7 0.14670887944574673 1103 8 0.2456141863769048 1103 9 0.19485217263795107 1103 10-11 0.2146382179014381 1103 12-13 0.26528176846436224 1103 14-15 0.4022421478531939 1103 16-17 0.28180933037108247 1103 18-19 0.26718942934957823 1103 20-21 0.3192153163267335 1103 22-23 0.23757215746616822 1103 24-25 0.23853655214585245 1103 26-27 0.3446997042645492 1103 28-29 0.22771509610653595 1103 30-31 0.25709740095567923 1103 32-33 0.31782157584800785 1103 34-35 0.42964141946367107 1103 36-37 0.38485693442798663 1103 38-39 0.3167113997145421 1103 40-41 0.37310974935284946 1103 42-43 0.39503575586650186 1103 44-45 0.5645074615210675 1103 46-47 0.5195585958428097 1103 48-49 0.6143390702463094 1103 50-51 0.6486666704734105 1103 52-53 0.7026050562432644 1103 54-55 0.8432664642801981 1103 56-57 1.0241163531736461 1103 58-59 1.083728968964877 1103 60-61 1.1687517844447015 1103 62-63 1.2891449173949958 1103 64-65 1.2934810130621024 1103 66-67 1.2131685860447483 1103 68-69 0.9564782013820015 1103 70-71 1.3825161546708031 1103 72-73 1.310117655694384 1103 74-75 1.2813336880341915 1103 76-77 1.3158791293784518 1103 78-79 1.4403230493747117 1103 80-81 1.5474683207529054 1103 82-83 1.8048174369571441 1103 84-85 1.8112255676665647 1103 86-87 1.775725726314949 1103 88-89 1.8186945064996678 1103 90-91 2.0106559793467866 1103 92-93 2.1194884968485255 1103 94-95 1.7592712675211004 1103 96-97 1.9852030794801792 1103 98-99 2.2117003082956828 1103 100-101 2.015779451830909 1104 1 0.13814557476675304 1104 2 0.18630382843547721 1104 3 0.1958569312201348 1104 4 0.1263087114738397 1104 5 0.1475967162472358 1104 6 0.12386471932840237 1104 7 0.07388507488640528 1104 8 0.06996074537467223 1104 9 0.143990128598233 1104 10-11 0.1618247568885849 1104 12-13 0.13096814406057433 1104 14-15 0.27771111257657566 1104 16-17 0.26266517701164815 1104 18-19 0.20499162757142386 1104 20-21 0.216834254836634 1104 22-23 0.2057582507424769 1104 24-25 0.1878163499884309 1104 26-27 0.24669773811784523 1104 28-29 0.1536256255204549 1104 30-31 0.27475398757442093 1104 32-33 0.48899595025711307 1104 34-35 0.4514616108738423 1104 36-37 0.4530103222395212 1104 38-39 0.42559359293918675 1104 40-41 0.5605420675892958 1104 42-43 0.4875493958728967 1104 44-45 0.6107194103903453 1104 46-47 0.7797135199110272 1104 48-49 0.7521263205159485 1104 50-51 0.8378754218993336 1104 52-53 0.8470598296863088 1104 54-55 1.0063194362222347 1104 56-57 1.1165027446452385 1104 58-59 1.2231704501869274 1104 60-61 1.078212337944457 1104 62-63 1.0875652352626481 1104 64-65 1.1812314389969139 1104 66-67 1.0886597817554495 1104 68-69 1.3430214710665176 1104 70-71 1.46230616867496 1104 72-73 1.5429886117686777 1104 74-75 1.633366418594079 1104 76-77 1.6112808064950457 1104 78-79 1.6944713788864938 1104 80-81 1.6846665180516887 1104 82-83 1.8511357672381514 1104 84-85 2.1287997223298625 1104 86-87 1.9783315799112096 1104 88-89 1.8378630730292365 1104 90-91 2.175801800984985 1104 92-93 2.413633373056026 1104 94-95 2.3334065639460846 1104 96-97 2.3761591319551307 1104 98-99 2.228971466876544 1104 100-101 1.8117375855627085 1105 1 0.0233590335930991 1105 2 0.056952607911739506 1105 3 0.05441008881786047 1105 4 -0.04647398567677641 1105 5 0.049589345957222974 1105 6 -0.11211713143560331 1105 7 0.09098896564434966 1105 8 -0.011389449663589346 1105 9 0.1696035370788067 1105 10-11 0.11911532417601478 1105 12-13 0.17055495285086408 1105 14-15 0.22533230092235357 1105 16-17 0.11673235146900396 1105 18-19 0.17106484867908733 1105 20-21 0.1397533910766171 1105 22-23 0.09111137264422808 1105 24-25 0.1274753244823259 1105 26-27 0.1086458819788092 1105 28-29 0.19195082409219566 1105 30-31 0.2782712273884371 1105 32-33 0.344230078702779 1105 34-35 0.3310369629073193 1105 36-37 0.27053271217779695 1105 38-39 0.22512446428203248 1105 40-41 0.3204362228378983 1105 42-43 0.34674528732824683 1105 44-45 0.38604433659975257 1105 46-47 0.3941587054424005 1105 48-49 0.5003536440297935 1105 50-51 0.595364612518928 1105 52-53 0.4204509058992798 1105 54-55 0.41967700361386306 1105 56-57 0.5987940341055378 1105 58-59 0.8709807962418452 1105 60-61 0.7607784141832141 1105 62-63 0.8040658279602475 1105 64-65 1.038783226529528 1105 66-67 1.0095114402254524 1105 68-69 1.1786673051091299 1105 70-71 1.0647680560015509 1105 72-73 1.2313410981583033 1105 74-75 1.1424606384255647 1105 76-77 1.143265327235337 1105 78-79 1.2536899414117073 1105 80-81 1.3033361569755684 1105 82-83 1.5753877251003985 1105 84-85 1.8028399029968796 1105 86-87 1.7678214112013109 1105 88-89 1.7425144595495716 1105 90-91 1.6975205053655813 1105 92-93 1.8596393443940151 1105 94-95 1.6455292930992798 1105 96-97 1.8798606548113845 1105 98-99 2.34285232589086 1105 100-101 2.2406039443773977 1106 1 -0.01175732376707117 1106 2 0.04058205814425264 1106 3 0.06064336629430045 1106 4 0.06636426465421863 1106 5 0.10127083103036938 1106 6 0.048405025829602266 1106 7 0.03789430956620521 1106 8 0.039718853249929964 1106 9 -0.050166034397499004 1106 10-11 0.015824896807977495 1106 12-13 0.02149009521056655 1106 14-15 0.09217560349671317 1106 16-17 0.04069005934332637 1106 18-19 0.1551032391966487 1106 20-21 0.0696683998319898 1106 22-23 0.173302717254451 1106 24-25 0.05506802221949414 1106 26-27 -0.1987294889000708 1106 28-29 0.15017032911806183 1106 30-31 0.21324840310651183 1106 32-33 0.09356224548962189 1106 34-35 0.09031515284252833 1106 36-37 0.07663208385056208 1106 38-39 0.11727169283386019 1106 40-41 0.20641587066312184 1106 42-43 0.2678432395492578 1106 44-45 0.27705085351635006 1106 46-47 -0.0693735060634495 1106 48-49 0.2032748255161252 1106 50-51 0.5021969488862865 1106 52-53 0.35303548889449843 1106 54-55 0.4162152999200188 1106 56-57 0.5842673898653672 1106 58-59 0.6584241123826473 1106 60-61 0.7653872358875162 1106 62-63 0.4974923217133984 1106 64-65 0.9041791907643635 1106 66-67 0.7552705454432562 1106 68-69 0.7048045623263484 1106 70-71 0.5986486110197511 1106 72-73 0.7998341679676919 1106 74-75 0.9420096798559676 1106 76-77 0.871188330142072 1106 78-79 1.026217080575762 1106 80-81 1.1724753285684457 1106 82-83 1.4948507954876185 1106 84-85 1.597419041829312 1106 86-87 1.424105625641534 1106 88-89 1.3379244173177405 1106 90-91 1.5403054822900692 1106 92-93 1.4996623570987353 1106 94-95 1.355135527130443 1106 96-97 1.6215953630449604 1106 98-99 1.73787526322646 1106 100-101 1.4572036790780931 1107 1 -0.01910654495808828 1107 2 -0.0376531047430646 1107 3 0.023920300943096606 1107 4 0.008512234094332882 1107 5 -0.06288018367051507 1107 6 0.018609623953928178 1107 7 0.037762770880540586 1107 8 0.05447800579457862 1107 9 0.04202356660994866 1107 10-11 0.019215096139681975 1107 12-13 0.05149079008592139 1107 14-15 0.23781593113653798 1107 16-17 0.0416987867339671 1107 18-19 0.034130316172991115 1107 20-21 0.03427784940537748 1107 22-23 0.14932628693886585 1107 24-25 0.06709265737945458 1107 26-27 0.15684828776699078 1107 28-29 0.17001798074413443 1107 30-31 0.21226033911458586 1107 32-33 0.1615094130191892 1107 34-35 0.23899702305688209 1107 36-37 0.19525693771339547 1107 38-39 0.2252320995675703 1107 40-41 0.2985178198494509 1107 42-43 0.2991869943874761 1107 44-45 0.5794008732044844 1107 46-47 0.5588150879842715 1107 48-49 0.7162559505071258 1107 50-51 0.6521715310772365 1107 52-53 0.5876569220200665 1107 54-55 0.6298439390249158 1107 56-57 0.5268800724377698 1107 58-59 0.6711853154191303 1107 60-61 0.7994181468691082 1107 62-63 0.8494140177944196 1107 64-65 1.03943765391951 1107 66-67 0.8607383611330555 1107 68-69 1.0964232683709128 1107 70-71 1.0795074160048124 1107 72-73 1.3735725729971264 1107 74-75 1.3565774369080437 1107 76-77 1.2945012764013875 1107 78-79 1.307697250417931 1107 80-81 1.5090300999849298 1107 82-83 1.730981022443661 1107 84-85 1.9317754470562356 1107 86-87 1.9225163725593966 1107 88-89 2.196719710695337 1107 90-91 2.290881070709304 1107 92-93 2.137418470478014 1107 94-95 2.207588498088313 1107 96-97 2.143467235343934 1107 98-99 2.143902880255048 1107 100-101 2.067629186611395 1108 1 -0.037045783399541676 1108 2 0.040712645106822265 1108 3 0.05736211357248777 1108 4 0.04765681128620258 1108 5 -0.014362062227704087 1108 6 -0.012385427746849587 1108 7 0.043761472471338436 1108 8 0.017324330453973857 1108 9 0.06167250869890495 1108 10-11 0.041785633020516855 1108 12-13 0.013916652057645251 1108 14-15 0.019909895669123046 1108 16-17 0.03532494143963305 1108 18-19 0.004074371308725233 1108 20-21 -0.014552862776945119 1108 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-0.5498930122340013 2316 54-55 -0.496587305253108 2316 56-57 -0.5181211736345048 2316 58-59 -0.4633662058382555 2316 60-61 -0.4873342657602464 2316 62-63 -0.627259202638708 2316 64-65 -0.5306335761459842 2316 66-67 -0.3448443491837878 2316 68-69 -0.22594753680397517 2316 70-71 -0.2948366866781491 2316 72-73 -0.18293798372250336 2316 74-75 0.046640122477910495 2316 76-77 0.1410173549777376 2316 78-79 -0.07506579756210385 2316 80-81 -0.6600922643128477 2316 82-83 -0.05125258038865965 2316 84-85 0.15964451963658988 2316 86-87 0.3080131168566389 2316 88-89 0.3664988269954925 2316 90-91 0.3568235222109202 2316 92-93 0.30069378501989874 2316 94-95 0.7979234333573899 2316 96-97 0.3323061293976153 2316 98-99 0.034324085693821615 2316 100-101 -0.32120259881802404 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 16.0 4 55.0 5 121.0 6 263.0 7 599.0 8 1397.0 9 2537.0 10 4055.0 11 6104.0 12 9171.0 13 12749.0 14 16925.0 15 22741.0 16 29165.0 17 37283.0 18 36201.0 19 31381.0 20 32615.0 21 35294.0 22 38990.0 23 43205.0 24 48907.0 25 55774.0 26 64482.0 27 74812.0 28 89161.0 29 103763.0 30 121346.0 31 141707.0 32 167272.0 33 200272.0 34 243931.0 35 299458.0 36 348135.0 37 314871.0 38 104219.0 39 2081.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.6259747557865 19.275989534489113 15.09489386702087 23.003141842703517 2 16.00541176006988 20.742270764600843 38.967883364018945 24.28443411131033 3 19.243055504885042 24.578733464184864 29.8054373919198 26.372773639010294 4 13.344771705606506 17.13406390068681 35.83437976320162 33.686784630505066 5 14.162310378806426 37.30093901732069 33.28121232064857 15.255538283224318 6 32.545805172148356 35.62281973509576 17.019619374360197 14.81175571839569 7 29.28422737723207 32.576596112607284 19.860995685620118 18.278180824540524 8 26.906731507643926 34.70413871980416 19.322343687581288 19.066786084970623 9 27.07985231990544 16.452978044989894 19.631784783988675 36.83538485111599 10-11 25.567206335053587 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28.63199020276575 22.26898720379484 36-37 22.796764648636834 25.863110292603352 28.680316151054814 22.659808907705 38-39 22.374251037642274 26.214626619357734 28.85941719084682 22.551705152153176 40-41 22.66545238410589 25.96351752263006 28.866922585473198 22.504107507790856 42-43 22.64412699987887 25.959131910645926 28.53283354907629 22.863907540398902 44-45 22.521411121068805 26.284379964469696 28.394345140102818 22.799863774358677 46-47 22.62486392145532 26.548303870321888 27.883515306464936 22.943316901757857 48-49 22.758609241805924 26.517566523389075 27.882108639485516 22.841715595319485 50-51 22.802004767488267 26.61918757155063 27.652873006341327 22.925934654619773 52-53 23.094737594789763 26.641733544540703 27.39529073725332 22.86823812341622 54-55 22.542032917120565 26.59293865963096 27.680216382969252 23.18481204027922 56-57 22.68279931968074 26.951156958643097 27.514259051040728 22.85178467063544 58-59 22.804795652494324 26.403140238140928 27.586366034236313 23.205698075128435 60-61 22.714356385435448 26.521707248201977 27.87988322791206 22.884053138450515 62-63 22.567315051881277 26.70499360467789 27.70854477783461 23.019146565606228 64-65 22.322815707979252 26.456719111600947 27.65526763224009 23.565197548179714 66-67 22.392791240157983 26.758776003683256 27.484992999043435 23.36343975711533 68-69 22.50783188990152 26.575380047770242 27.54740268008057 23.369385382247668 70-71 22.406742925333234 26.734749680370644 27.347777716366135 23.510729677929987 72-73 22.597918323568255 26.600165629355065 27.269851809154122 23.532064237922555 74-75 22.381456703340962 26.825603615527683 27.046995584929366 23.74594409620199 76-77 22.40055511250003 26.598301536701413 27.424932489768576 23.576210861029978 78-79 22.47149466667348 26.659299703108207 27.32394987935351 23.54525575086481 80-81 22.217195783458344 27.05725500868915 27.23727650765953 23.488272700192972 82-83 22.434131188742356 26.843030672167504 27.05112721086611 23.671710928224023 84-85 22.25319127821875 26.520408565336744 27.143048945823793 24.083351210620716 86-87 22.387594127604928 26.443180491506624 27.25523174362267 23.913993637265776 88-89 22.22595150937628 26.53551574460447 27.049713505621536 24.188819240397713 90-91 22.13750149578699 26.532561458603915 27.276934380919304 24.053002664689796 92-93 22.25689661210862 26.19154807490252 27.24519309346471 24.306362219524154 94-95 22.029407907253585 26.076538484760764 27.228426318159855 24.6656272898258 96-97 22.170826970237126 26.06781449260981 27.145223792495326 24.616134744657735 98-99 21.935462334726708 26.074290075466987 26.92452498407554 25.065722605730762 100-101 22.100031228749128 25.7320879775153 27.19080984610356 24.977070947632015 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1258.0 1 893.0 2 683.0 3 850.0 4 778.5 5 784.0 6 1324.0 7 2752.5 8 4196.0 9 4619.5 10 3916.5 11 3146.5 12 3176.5 13 3404.0 14 3643.0 15 3922.5 16 4116.0 17 4286.5 18 4506.0 19 4753.5 20 5199.0 21 5937.0 22 6852.5 23 7856.5 24 9069.5 25 10885.0 26 13271.5 27 15948.5 28 18544.0 29 21743.5 30 25972.0 31 30370.5 32 35319.0 33 39626.5 34 42977.0 35 47247.0 36 52554.0 37 57750.0 38 62262.0 39 64805.0 40 67966.5 41 75597.0 42 83675.5 43 90253.5 44 94939.0 45 98097.0 46 99088.5 47 102223.0 48 110565.0 49 114891.5 50 114465.0 51 111794.5 52 106994.5 53 105043.0 54 103226.5 55 95001.0 56 84598.0 57 75843.5 58 67991.5 59 59815.0 60 50595.0 61 41689.0 62 34170.0 63 26705.0 64 20693.5 65 16558.0 66 12706.0 67 10362.5 68 8024.5 69 5521.5 70 4271.5 71 3565.0 72 3104.0 73 2770.0 74 2334.5 75 2011.5 76 1422.5 77 784.0 78 448.0 79 285.0 80 186.5 81 103.5 82 58.5 83 26.0 84 13.5 85 7.0 86 4.0 87 3.5 88 1.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02320265181502379 2 0.013425433754604962 3 0.0016781792193256203 4 0.0 5 3.2833941247675173E-4 6 0.0 7 0.0 8 3.64821569418613E-5 9 2.188929416511678E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0019517953963895799 26-27 0.009266467863232772 28-29 2.918572555348904E-4 30-31 0.0 32-33 2.553750985930291E-4 34-35 0.010671030905494431 36-37 0.001258634414494215 38-39 0.013042371106715417 40-41 0.012878201400477041 42-43 0.005472323541279196 44-45 9.302950020174633E-4 46-47 7.29643138837226E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.824107847093065E-5 66-67 4.377858833023356E-4 68-69 0.0010579825513139778 70-71 1.824107847093065E-5 72-73 2.188929416511678E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.6416970623837587E-4 82-83 0.0011127057867267697 84-85 0.005764180796814087 86-87 0.0038671086358372984 88-89 0.0 90-91 0.0012403933360232843 92-93 3.6482156941861305E-4 94-95 1.824107847093065E-5 96-97 0.0 98-99 0.0 100-101 7.29643138837226E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2741066.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.978670170382216 #Duplication Level Percentage of deduplicated Percentage of total 1 70.27811106880196 30.204597558235267 2 14.998649091409536 12.892439846019865 3 5.763044146464765 7.430639206447831 4 2.7885297121678443 4.793891950382906 5 1.6026038364214619 3.4438890849673593 6 0.9665787630354863 2.492536191011892 7 0.6903688511284365 2.076979460398834 8 0.4554310648408464 1.5659057216912555 9 0.36119973100975455 1.3971495694229115 >10 1.8145991266684396 14.38568833675213 >50 0.1577897000651137 4.697128327342456 >100 0.11127604104552061 9.277726392319332 >500 0.008672837611239112 2.4930929103112223 >1k 0.002890945870407509 1.773946954722222 >5k 1.7005563943573584E-4 0.6293577746374577 >10k+ 8.502781971786792E-5 0.4450307153370665 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12178 0.44427970723798693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9953 0.3631069080423456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7269 0.2651887988103898 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 2896 0.10565232650363034 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.64821569418613E-5 0.0 0.0 0.0 0.0 3 3.64821569418613E-5 0.0 0.0 0.0 0.0 4 3.64821569418613E-5 0.0 0.0 0.0 0.0 5 3.64821569418613E-5 0.0 0.0 0.0 0.0 6 3.64821569418613E-5 0.0 0.0 0.0 0.0 7 1.8241078470930652E-4 0.0 0.0 0.0 0.0 8 1.8241078470930652E-4 0.0 0.0 0.0 0.0 9 1.8241078470930652E-4 0.0 0.0 0.0 0.0 10-11 2.0065186318023718E-4 0.0 0.0 0.0 0.0 12-13 2.371340201220985E-4 0.0 0.0 3.64821569418613E-5 0.0 14-15 3.6482156941861305E-4 0.0 0.0 3.64821569418613E-5 0.0 16-17 3.6482156941861305E-4 0.0 0.0 3.64821569418613E-5 0.0 18-19 4.19544804831405E-4 0.0 0.0 3.64821569418613E-5 3.64821569418613E-5 20-21 4.377858833023357E-4 0.0 0.0 1.2768754929651455E-4 3.64821569418613E-5 22-23 4.377858833023357E-4 0.0 0.0 3.1009833400582113E-4 3.64821569418613E-5 24-25 5.837145110697808E-4 0.0 0.0 8.938128450756021E-4 3.64821569418613E-5 26-27 5.837145110697808E-4 0.0 0.0 0.0018605900040349265 3.64821569418613E-5 28-29 6.201966680116421E-4 0.0 0.0 0.005125743050331514 3.64821569418613E-5 30-31 6.201966680116421E-4 0.0 0.0 0.015377229150994539 3.64821569418613E-5 32-33 6.201966680116421E-4 0.0 0.0 0.03584371919537873 3.64821569418613E-5 34-35 7.661252957790874E-4 0.0 0.0 0.06471934641486196 3.64821569418613E-5 36-37 9.850182374302552E-4 0.0 0.0 0.1155754731918166 3.64821569418613E-5 38-39 0.00111270578672677 0.0 0.0 0.19216976169125444 3.64821569418613E-5 40-41 0.0011309468651977005 0.0 0.0 0.2765347496193087 3.64821569418613E-5 42-43 0.0011856701006104924 0.0 0.0 0.3605531570564153 3.64821569418613E-5 44-45 0.0013498398068488683 0.0 0.0 0.46728170718983053 3.64821569418613E-5 46-47 0.0014957684346163136 0.0 0.0 0.5943308187398625 3.64821569418613E-5 48-49 0.0015504916700291053 0.0 0.0 0.732032720116918 3.64821569418613E-5 50-51 0.001568732748500036 0.0 0.0 0.8797672146529854 3.64821569418613E-5 52-53 0.001568732748500036 3.64821569418613E-5 0.0 1.023999422122634 3.64821569418613E-5 54-55 0.0016052149054418974 3.64821569418613E-5 0.0 1.1843020197251726 3.64821569418613E-5 56-57 0.0016052149054418974 3.64821569418613E-5 0.0 1.368828769537107 3.64821569418613E-5 58-59 0.0016052149054418974 3.64821569418613E-5 0.0 1.5723992052726932 3.64821569418613E-5 60-61 0.0016052149054418974 3.64821569418613E-5 0.0 1.7931892190848377 3.64821569418613E-5 62-63 0.0016052149054418974 3.64821569418613E-5 0.0 2.012793562796372 3.64821569418613E-5 64-65 0.0016052149054418974 3.64821569418613E-5 0.0 2.2416643743711386 3.64821569418613E-5 66-67 0.00167817921932562 3.64821569418613E-5 0.0 2.508075325439081 3.64821569418613E-5 68-69 0.0016964202977965506 3.64821569418613E-5 0.0 2.7919612296821747 5.472323541279195E-5 70-71 0.0018241078470930651 3.64821569418613E-5 0.0 3.108115601740345 7.29643138837226E-5 72-73 0.0018788310825058573 3.64821569418613E-5 0.0 3.4259116708608985 7.29643138837226E-5 74-75 0.001897072160976788 3.64821569418613E-5 0.0 3.754415253043889 1.0944647082558392E-4 76-77 0.0019517953963895797 3.64821569418613E-5 0.0 4.105902594100252 1.0944647082558392E-4 78-79 0.0019700364748605104 3.64821569418613E-5 0.0 4.495459065925447 1.0944647082558392E-4 80-81 0.0024443045151047076 3.64821569418613E-5 0.0 4.915441656640153 1.0944647082558392E-4 82-83 0.0024443045151047076 3.64821569418613E-5 0.0 5.342155205310634 1.0944647082558392E-4 84-85 0.0024990277505174997 3.64821569418613E-5 0.0 5.774505247228633 1.6416970623837587E-4 86-87 0.002571992064401222 3.64821569418613E-5 0.0 6.214315890241242 1.8241078470930652E-4 88-89 0.002608474221343083 3.64821569418613E-5 0.0 6.685701840087032 1.8241078470930652E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6675 0.0 52.45975 1 GTATCAA 11655 0.0 40.521374 1 ATCAACG 15450 0.0 30.435614 3 TATCAAC 15660 0.0 30.05945 2 TCAACGC 15900 0.0 29.604103 4 CAACGCA 16120 0.0 29.170609 5 AACGCAG 16530 0.0 28.475813 6 ACGCAGA 19110 0.0 24.581644 7 GTACATG 14130 0.0 24.546566 1 CGCAGAG 19275 0.0 24.39586 8 TACATGG 14195 0.0 23.658278 2 TGCACCG 675 0.0 23.221245 5 ACATGGG 14735 0.0 22.403196 3 GCAGAGT 20790 0.0 22.206858 9 GCACCGT 715 0.0 21.922153 6 GAGTACT 10925 0.0 21.912123 12-13 TAAGGTG 1115 0.0 21.725544 5 GTAAGGT 905 0.0 21.518433 4 AGTACTT 11215 0.0 21.493746 12-13 ATTCGCG 325 0.0 21.192188 80-81 >>END_MODULE