FastQCFastQC Report
Thu 26 May 2016
SRR938323_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938323_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527702
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110510.43719552383943994No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85180.3369859263473305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60790.2404951216559547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67400.045.2956471
GTATCAA110700.038.704231
TATCAAC139750.030.5220222
ATCAACG140650.030.2590983
TCAACGC142200.029.9292724
CAACGCA144450.029.4301625
AACGCAG148800.028.5169416
ACGCAGA164800.025.6344957
CGCAGAG166200.025.3894658
TAAGGTG15700.023.6247735
GCAGAGT175700.023.1777949
GTACATG105050.023.041091
TACATGG104400.022.774152
GAGTACT98550.022.56364612-13
GGTAAGG15450.021.8525713
GTAAGGT15800.021.3684964
ACATGGG108350.021.329473
GTACTTT105500.021.11954514-15
CAGAGTA170350.020.30710210-11
AGTACTT104100.020.24244112-13
Produced by FastQC (version 0.11.5)