##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938323_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2527702 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.273904914424246 31.0 31.0 34.0 28.0 34.0 2 31.333738312506775 31.0 31.0 34.0 28.0 34.0 3 31.36841407729234 31.0 31.0 34.0 28.0 34.0 4 35.02609287012472 37.0 35.0 37.0 32.0 37.0 5 35.015317865792724 37.0 35.0 37.0 32.0 37.0 6 35.034397250941765 37.0 35.0 37.0 32.0 37.0 7 34.95883652424218 37.0 35.0 37.0 32.0 37.0 8 34.92859878260966 37.0 35.0 37.0 32.0 37.0 9 36.441687746419476 39.0 37.0 39.0 32.0 39.0 10-11 36.28683029091246 39.0 35.0 39.0 32.0 39.0 12-13 36.158833003257506 39.0 35.0 39.0 32.0 39.0 14-15 37.13674594552681 39.0 36.0 41.0 31.0 41.0 16-17 36.976279442750766 39.0 36.0 41.0 31.0 41.0 18-19 37.15494963409452 39.5 36.5 41.0 31.0 41.0 20-21 37.15497198641296 40.0 37.0 41.0 31.0 41.0 22-23 37.065843402426395 39.0 36.0 41.0 31.0 41.0 24-25 36.943065677837026 39.0 36.0 41.0 30.5 41.0 26-27 36.7327177016911 39.0 36.0 41.0 30.0 41.0 28-29 36.53476220693737 39.0 36.0 41.0 30.0 41.0 30-31 36.27483382139192 39.0 35.5 40.0 29.5 41.0 32-33 35.99249812675703 38.5 35.0 40.0 29.0 41.0 34-35 35.74298374571053 38.0 35.0 40.0 27.5 41.0 36-37 35.467444936151495 38.0 34.5 40.0 27.0 41.0 38-39 35.319333924647765 38.0 34.0 40.0 26.0 41.0 40-41 35.207763019533154 38.0 34.0 40.0 25.5 41.0 42-43 34.910142888679125 38.0 34.0 40.0 24.5 41.0 44-45 34.5491517591868 38.0 33.0 40.0 24.0 41.0 46-47 34.136796188791244 38.0 33.0 40.0 23.0 41.0 48-49 33.75878802168927 38.0 33.0 40.0 21.0 41.0 50-51 33.06213984085149 37.5 32.0 39.5 19.0 40.5 52-53 33.089238565305564 37.0 32.0 39.5 19.0 40.0 54-55 33.20682936517042 37.5 32.5 40.0 18.0 41.0 56-57 32.90760758190641 37.0 32.0 40.0 14.5 41.0 58-59 32.55902792338654 37.0 31.0 40.0 12.0 41.0 60-61 32.10815139601108 36.0 31.0 39.0 8.0 41.0 62-63 31.568334004562246 36.0 30.5 39.0 7.0 40.0 64-65 31.077778947043598 35.0 29.5 39.0 2.0 40.0 66-67 30.56544244535155 35.0 29.0 38.0 2.0 40.0 68-69 29.998598925031512 34.5 28.5 38.0 2.0 40.0 70-71 29.382692659182133 34.0 28.0 37.0 2.0 39.5 72-73 28.75511947215297 34.0 26.0 36.5 2.0 39.0 74-75 28.0963588666702 34.0 26.0 36.0 2.0 39.0 76-77 27.352942910200646 33.0 25.0 35.5 2.0 38.0 78-79 26.583100776911202 32.0 23.0 35.0 2.0 37.0 80-81 25.90428222947167 32.0 21.0 35.0 2.0 36.5 82-83 25.1749369585497 31.0 19.0 35.0 2.0 36.0 84-85 24.473465819942383 31.0 14.5 34.0 2.0 36.0 86-87 23.729536353573323 30.5 7.0 34.0 2.0 35.0 88-89 22.919513257496334 30.0 2.0 34.0 2.0 35.0 90-91 22.019194905095617 29.0 2.0 34.0 2.0 35.0 92-93 21.094469403434424 29.0 2.0 34.0 2.0 35.0 94-95 20.10815554998176 27.5 2.0 34.0 2.0 35.0 96-97 18.87487646882425 25.5 2.0 33.0 2.0 35.0 98-99 17.380797855126907 21.5 2.0 32.5 2.0 34.0 100-101 13.743932433490972 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5434190221486652 1101 2 0.4426407330771056 1101 3 0.4279335102438786 1101 4 0.2595081865669542 1101 5 0.4017741397353731 1101 6 0.5075069036306132 1101 7 0.5271389061595642 1101 8 0.5336462234453379 1101 9 0.6368008487927668 1101 10-11 0.664468193579502 1101 12-13 0.7889132070383909 1101 14-15 0.8884178920535248 1101 16-17 0.7728136737749267 1101 18-19 0.8504560821260014 1101 20-21 0.7743129029237323 1101 22-23 0.8400711844524054 1101 24-25 0.9102648563738143 1101 26-27 1.0203078923866116 1101 28-29 1.0396897925237738 1101 30-31 0.9410833588933301 1101 32-33 0.9014751727490236 1101 34-35 0.9593678619911614 1101 36-37 0.9349759018489294 1101 38-39 0.9711733493029797 1101 40-41 0.9378729756032342 1101 42-43 1.2197060803513722 1101 44-45 1.2046396094145138 1101 46-47 1.067088462919017 1101 48-49 1.0645149605562452 1101 50-51 0.9733763526101882 1101 52-53 1.0634404167568405 1101 54-55 1.1021014207587427 1101 56-57 1.1169345293051265 1101 58-59 1.0611277679284257 1101 60-61 1.2551499911761113 1101 62-63 0.9971267223250599 1101 64-65 1.074081902817305 1101 66-67 1.142047775283654 1101 68-69 1.239831987661951 1101 70-71 1.2200809752812383 1101 72-73 1.2776624633558598 1101 74-75 0.915022198203463 1101 76-77 1.0389012743612334 1101 78-79 1.3006829300767997 1101 80-81 1.3608090204632646 1101 82-83 1.2352218874051495 1101 84-85 1.1772825102329456 1101 86-87 1.4097246251759792 1101 88-89 1.3390128043083713 1101 90-91 1.1825532249564858 1101 92-93 1.205925328987174 1101 94-95 1.3331293074134862 1101 96-97 1.139859515905652 1101 98-99 1.2940253583133519 1101 100-101 1.3113688762734714 1102 1 0.43249068344394104 1102 2 0.37910407417737346 1102 3 0.3491146214751488 1102 4 0.23357163754459975 1102 5 0.3348002827858423 1102 6 0.3945124273436207 1102 7 0.3738829063059015 1102 8 0.42053798684570864 1102 9 0.5184463203140197 1102 10-11 0.7143424635640159 1102 12-13 0.5465718914428876 1102 14-15 0.6977304973700598 1102 16-17 0.7228266061884696 1102 18-19 0.7307170152301268 1102 20-21 0.761087183530158 1102 22-23 0.7946942976597953 1102 24-25 0.8147082796318088 1102 26-27 0.7008634791411339 1102 28-29 0.6350894474240789 1102 30-31 0.7890607518792123 1102 32-33 0.7868425640052337 1102 34-35 0.8196965121648887 1102 36-37 0.9487567041784359 1102 38-39 0.7296387852616917 1102 40-41 0.9291266688020983 1102 42-43 1.0023331477324575 1102 44-45 0.9808985060646336 1102 46-47 1.1063606408048443 1102 48-49 1.2272952176705942 1102 50-51 0.9946751231323745 1102 52-53 0.7454821881316676 1102 54-55 0.9087784451227066 1102 56-57 0.8605274490172974 1102 58-59 0.870052481064711 1102 60-61 0.8079476123821863 1102 62-63 0.9847019782215902 1102 64-65 1.136038994160259 1102 66-67 0.7795271131055692 1102 68-69 0.8786541464379738 1102 70-71 0.9260656020752087 1102 72-73 1.045191740190056 1102 74-75 1.142101260176684 1102 76-77 1.109200895598761 1102 78-79 1.0702752156835373 1102 80-81 1.2214226329998148 1102 82-83 1.3891218604813425 1102 84-85 1.3188049922551244 1102 86-87 1.2309140317643212 1102 88-89 1.1867008225074507 1102 90-91 1.0988478574272413 1102 92-93 1.1508578766630144 1102 94-95 1.2973317128424071 1102 96-97 1.600805391990022 1102 98-99 1.8322691205096895 1102 100-101 1.6527100638284455 1103 1 0.25834778042773365 1103 2 0.18124863544356629 1103 3 0.27312033001952685 1103 4 0.1535196823901046 1103 5 0.17591309144987832 1103 6 0.3431411725175124 1103 7 0.3085041743026764 1103 8 0.3825415466894668 1103 9 0.44783224726572257 1103 10-11 0.5179894218920182 1103 12-13 0.5680502739843547 1103 14-15 0.6472511634045404 1103 16-17 0.6644462817886136 1103 18-19 0.6480137573082771 1103 20-21 0.45128207494112615 1103 22-23 0.5044996884774591 1103 24-25 0.6133383889107407 1103 26-27 0.6023565156201158 1103 28-29 0.5451682707439431 1103 30-31 0.7450291661055388 1103 32-33 0.756563352087916 1103 34-35 0.7569391353357346 1103 36-37 0.7031196451763435 1103 38-39 0.6947793163030767 1103 40-41 0.7275411867681427 1103 42-43 0.9175136454834103 1103 44-45 0.7761936137254466 1103 46-47 0.643325927310805 1103 48-49 0.744709743162737 1103 50-51 0.8463448504117181 1103 52-53 0.8790376353950577 1103 54-55 0.9741941020380551 1103 56-57 0.897452125959596 1103 58-59 0.9719076952699623 1103 60-61 1.046387841376223 1103 62-63 1.1859345176771505 1103 64-65 1.1676808999686443 1103 66-67 1.1872113833125155 1103 68-69 1.2782150143725346 1103 70-71 1.3356825896044668 1103 72-73 1.381105897530631 1103 74-75 1.3223701707490925 1103 76-77 1.3052419962402375 1103 78-79 1.0665715921188266 1103 80-81 1.19939061196688 1103 82-83 1.318133677707575 1103 84-85 1.2667220509059973 1103 86-87 1.3790991919322018 1103 88-89 1.241920186276321 1103 90-91 1.2262833336911854 1103 92-93 1.1301234801430802 1103 94-95 1.2777949064662977 1103 96-97 1.3686854379242988 1103 98-99 1.39219982083808 1103 100-101 1.29851054888538 1104 1 0.382476326991835 1104 2 0.305087794826175 1104 3 0.3832246710068077 1104 4 0.2936004354790924 1104 5 0.4300267586647948 1104 6 0.5224600741988894 1104 7 0.36399783232423744 1104 8 0.5038767737581509 1104 9 0.558950191545371 1104 10-11 0.7177410935743254 1104 12-13 0.6305122676016808 1104 14-15 0.7337871330042205 1104 16-17 0.7736877731470955 1104 18-19 0.7433956376605977 1104 20-21 0.6920967252943697 1104 22-23 0.7413592809293306 1104 24-25 0.6859548949330971 1104 26-27 0.7021113744518459 1104 28-29 0.7344717783440302 1104 30-31 0.9077660625821409 1104 32-33 0.9137354557621435 1104 34-35 0.8204622402237689 1104 36-37 0.9626706192170928 1104 38-39 0.9074245784932558 1104 40-41 0.9930109067538737 1104 42-43 1.1527184639745016 1104 44-45 0.9783798635548635 1104 46-47 0.8849361679660177 1104 48-49 0.9130513092172308 1104 50-51 0.8854222948505708 1104 52-53 1.0267228072796613 1104 54-55 1.1143885001965117 1104 56-57 1.125842579594206 1104 58-59 1.1966593370259915 1104 60-61 1.058243737931825 1104 62-63 1.1944188440843426 1104 64-65 1.3196277245503438 1104 66-67 1.3985083167061774 1104 68-69 1.5774673210906265 1104 70-71 1.7239943251375003 1104 72-73 1.537448594200196 1104 74-75 1.4730459511667675 1104 76-77 1.5097443909416093 1104 78-79 1.6192042972395733 1104 80-81 1.6391950855874562 1104 82-83 1.7600127910755248 1104 84-85 1.6715156134028568 1104 86-87 1.713897042865785 1104 88-89 1.8358985238352439 1104 90-91 1.689409511290524 1104 92-93 1.9218401237066551 1104 94-95 1.89827723439614 1104 96-97 2.0884587672594783 1104 98-99 2.2273672613984914 1104 100-101 1.7425718121816285 1105 1 0.4021850023626108 1105 2 0.3126102718445587 1105 3 0.30705274770705415 1105 4 0.2460779641785038 1105 5 0.18433386813102715 1105 6 0.30358265219357605 1105 7 0.3412285630258509 1105 8 0.3148715546957632 1105 9 0.5941972668563409 1105 10-11 0.49951206977814167 1105 12-13 0.5132879179618044 1105 14-15 0.6799449233385957 1105 16-17 0.6844473239054807 1105 18-19 0.5967698742095848 1105 20-21 0.6471332541568771 1105 22-23 0.7122705591557761 1105 24-25 0.6677853791118835 1105 26-27 0.5905036170591274 1105 28-29 0.6482327438284017 1105 30-31 0.7035896691084886 1105 32-33 0.7906359979665609 1105 34-35 0.7920641568865889 1105 36-37 0.9136712408845966 1105 38-39 0.8184131985043166 1105 40-41 0.9631563575099946 1105 42-43 1.144721767145974 1105 44-45 1.1873005278313968 1105 46-47 1.1150400560538714 1105 48-49 0.9945785929018882 1105 50-51 1.013522538128548 1105 52-53 1.188935000167838 1105 54-55 1.147674013685446 1105 56-57 1.2969441604079037 1105 58-59 1.334770871524114 1105 60-61 1.2625237664807898 1105 62-63 1.2664909638047241 1105 64-65 1.3384324876469904 1105 66-67 1.4241131846100537 1105 68-69 1.198637951453474 1105 70-71 1.3909572213504724 1105 72-73 1.3902936486129 1105 74-75 1.5222023545064758 1105 76-77 1.4886211325290475 1105 78-79 1.668002480581027 1105 80-81 1.748097324262325 1105 82-83 1.7718780065843056 1105 84-85 1.8452195781663576 1105 86-87 1.976571290950428 1105 88-89 1.6945773038459997 1105 90-91 1.4534017831157477 1105 92-93 1.7653270737635545 1105 94-95 1.8081612474492985 1105 96-97 2.0593052199595014 1105 98-99 2.0940226544748946 1105 100-101 1.9440494768216698 1106 1 0.16985949943276069 1106 2 0.1323033821665227 1106 3 0.03081738775494003 1106 4 0.1322560900127172 1106 5 0.29806132181121114 1106 6 0.02470958234537335 1106 7 0.28110769036688765 1106 8 0.24207942399355176 1106 9 0.004201622614829148 1106 10-11 0.06303287397503254 1106 12-13 0.07868796413256263 1106 14-15 0.36168587828197474 1106 16-17 0.18299434566825568 1106 18-19 0.05806173413867555 1106 20-21 0.2765640328963386 1106 22-23 0.2601132315269368 1106 24-25 0.12733450252451206 1106 26-27 0.013279065365139786 1106 28-29 0.30380428156706785 1106 30-31 -0.038656626006350336 1106 32-33 0.13122830315898426 1106 34-35 -0.1024389249894 1106 36-37 -5.420451749671429E-4 1106 38-39 0.05383162202003433 1106 40-41 0.4511260470903764 1106 42-43 -0.09034322923096028 1106 44-45 0.2626268523927209 1106 46-47 0.25571308065531184 1106 48-49 0.3821466777960083 1106 50-51 0.07129733603044741 1106 52-53 0.2048888138242475 1106 54-55 0.2641639884387956 1106 56-57 -0.5872990673405099 1106 58-59 -0.2515717538533693 1106 60-61 0.4403410016266349 1106 62-63 0.34630348860995497 1106 64-65 0.25607306408549846 1106 66-67 -0.053078998470979855 1106 68-69 -0.0340988725214757 1106 70-71 -0.1377594555450763 1106 72-73 0.1716781012548907 1106 74-75 0.4550348117072147 1106 76-77 0.3453261347791141 1106 78-79 0.5258674543778987 1106 80-81 0.7790860121395298 1106 82-83 0.4834896184560513 1106 84-85 0.4264932383302842 1106 86-87 0.38368174544935485 1106 88-89 0.5563137341135089 1106 90-91 0.7762319202290193 1106 92-93 0.6796218588508331 1106 94-95 0.5631758920088643 1106 96-97 0.8333382293035214 1106 98-99 0.8290644066650117 1106 100-101 0.3098652618767552 1107 1 0.23334287784897612 1107 2 0.08098039434874948 1107 3 0.1601500326431058 1107 4 0.026896578637526147 1107 5 -0.014033820625868998 1107 6 0.23256350866632403 1107 7 0.15106048392154037 1107 8 0.0808946304115068 1107 9 0.2782319124896304 1107 10-11 0.2529052376110812 1107 12-13 0.3777820679079582 1107 14-15 0.38582684171132087 1107 16-17 0.49639594981399426 1107 18-19 0.38466517345317186 1107 20-21 0.4821092275233809 1107 22-23 0.3904556388073317 1107 24-25 0.43684861779112794 1107 26-27 0.35625362800122673 1107 28-29 0.5106786291924834 1107 30-31 0.41277352463813344 1107 32-33 0.507621783823005 1107 34-35 0.5211106520535296 1107 36-37 0.5427300302388858 1107 38-39 0.6127110233810029 1107 40-41 0.511372919759701 1107 42-43 0.5655465733034433 1107 44-45 0.3717987145084578 1107 46-47 0.3781450046454822 1107 48-49 0.37634091866105024 1107 50-51 0.26774075465734626 1107 52-53 0.4110961231596164 1107 54-55 0.5529282036755205 1107 56-57 0.6237661046123719 1107 58-59 0.5253011552299114 1107 60-61 0.325440873270999 1107 62-63 0.5676987631702808 1107 64-65 0.5289886978703464 1107 66-67 0.5954526182020672 1107 68-69 0.6545303937620943 1107 70-71 0.7554138057241424 1107 72-73 0.6943246505947442 1107 74-75 0.6759148227360363 1107 76-77 0.8526171030464162 1107 78-79 1.0578861485755233 1107 80-81 1.069069563095173 1107 82-83 0.8525383995912854 1107 84-85 0.8917316809714109 1107 86-87 0.9948722760744637 1107 88-89 1.1192475979948817 1107 90-91 0.85639339658427 1107 92-93 0.7326469074658526 1107 94-95 0.7803600057084985 1107 96-97 1.164305246443579 1107 98-99 1.222763634904279 1107 100-101 1.2779420423509436 1108 1 0.28578808945852785 1108 2 0.10562072211966367 1108 3 0.27930384877612724 1108 4 0.2193712767428906 1108 5 0.221804451850943 1108 6 0.15816620286276617 1108 7 0.27111621831586774 1108 8 0.3211588445613529 1108 9 0.14935750026253913 1108 10-11 0.30833873346774254 1108 12-13 0.23367176512737586 1108 14-15 0.2924091352881888 1108 16-17 0.35267521128946555 1108 18-19 0.558203403172584 1108 20-21 0.4334366567751502 1108 22-23 0.3473737460987323 1108 24-25 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9432.0 11 12158.0 12 15088.0 13 18429.0 14 21668.0 15 25480.0 16 30216.0 17 35021.0 18 32813.0 19 29559.0 20 30818.0 21 33119.0 22 36964.0 23 40473.0 24 45741.0 25 51154.0 26 57693.0 27 65929.0 28 75253.0 29 86504.0 30 100228.0 31 117205.0 32 139665.0 33 166866.0 34 204194.0 35 251016.0 36 297906.0 37 300279.0 38 148057.0 39 7855.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41677556732197 19.38795655684812 15.670220725110214 22.525047150719693 2 15.134434492787602 20.488092333915226 40.634098663971585 23.743374509325584 3 18.800894152836133 24.761490592701026 30.2572669721108 26.180348282352046 4 12.624110216684912 17.351269738415983 37.77188593663111 32.252734108267994 5 13.518139820909859 37.62557171708686 34.326680421292544 14.529608040710734 6 31.08126462949052 36.843922665607515 17.811002525251528 14.263810179650442 7 27.87095497670185 33.23932291924298 20.900505535164868 17.989216568890296 8 26.17520974334561 34.75085446595729 20.189060857129178 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21.34146051840986 26.816346305322398 29.66349986423466 22.178693312033086 34-35 22.111450915641363 26.9975699940482 29.433731617847176 21.457247472463266 36-37 22.14148278301168 26.51463304479033 29.474095451538986 21.869788720659 38-39 21.58733368646814 26.911167337418323 29.4504532545224 22.051045721591134 40-41 22.100733398409094 26.694174466226727 29.471404229502273 21.733687905861903 42-43 21.78996457302421 26.744220559043374 29.264474721475587 22.20134014645683 44-45 21.85126329680695 26.983005368672657 29.263469702775453 21.902261631744942 46-47 22.16573233818464 27.30640190915981 28.58518921363483 21.94267653902072 48-49 22.068428797994528 27.221508624074847 28.469862474151714 22.24020010377891 50-51 22.193843285095294 27.41920328007848 28.397818318928124 21.989135115898097 52-53 22.324006506931767 27.32782753900806 28.42477927926413 21.923386674796046 54-55 21.927610784770092 27.439405452445946 28.484892486828045 22.148091275955913 56-57 22.05384680822441 27.5189278910165 28.345483717166765 22.081741583592333 58-59 22.101891849862835 27.155945551001125 28.34953500086459 22.392627598271453 60-61 21.800872944267695 27.36899900280165 28.635698117985974 22.19442993494468 62-63 21.87141249925567 27.382585174673885 28.675593886854223 22.070408439216227 64-65 21.633482071851862 27.328684957890832 28.56270163380255 22.47513133645476 66-67 21.587263466482977 27.377996054947452 28.534607440366738 22.500133038202836 68-69 22.046451524070697 27.137020561373326 28.540196699465735 22.276331215090238 70-71 21.891397217555795 27.185184305764142 28.24242208384532 22.68099639283474 72-73 21.88517819036586 27.204583295821827 28.237249468490834 22.672989045321483 74-75 21.931544947866456 27.612514107793835 28.014556552268882 22.441384392070827 76-77 22.02547008019399 27.213051051749776 28.178694973966433 22.582783894089797 78-79 22.01742687009093 27.371042229537927 28.07222371024426 22.539307190126884 80-81 22.189967142679105 27.51864915067485 27.774643485210248 22.51674022143579 82-83 22.215911788444192 27.45305749418411 27.8602486192217 22.470782098149993 84-85 22.135035456727916 27.442526234497077 27.862294013106563 22.560144295668437 86-87 22.222842099619704 27.476283259966888 27.667548409292387 22.633326231121025 88-89 22.177498826563284 27.403685184936 27.55472805950373 22.864087928996987 90-91 22.13560495879216 27.440641912281222 27.60210936649748 22.821643762429137 92-93 22.294951245765663 27.097085034982747 27.764942856238328 22.843020863013265 94-95 22.05080130669363 26.924858783560612 27.901732396158447 23.122607513587315 96-97 22.315884253281286 26.68105337627578 27.84689137242679 23.156170998016137 98-99 22.13277679573796 26.824724293475445 27.4772432552859 23.565255655500696 100-101 22.681551727175496 26.20686911124937 27.87785545699882 23.233723704576313 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 964.0 1 628.0 2 410.5 3 571.0 4 538.0 5 532.5 6 937.0 7 2114.5 8 3361.5 9 3753.5 10 3190.0 11 2505.0 12 2394.0 13 2498.0 14 2726.0 15 2917.5 16 3089.5 17 3411.0 18 3763.5 19 4173.0 20 4730.5 21 5676.0 22 6919.5 23 8377.5 24 10127.5 25 12394.5 26 15632.5 27 19766.5 28 23577.5 29 27597.0 30 32376.5 31 38486.5 32 46090.5 33 51002.5 34 53541.5 35 57214.0 36 61997.0 37 66453.0 38 69766.0 39 72706.0 40 76578.5 41 81444.5 42 85184.5 43 87528.5 44 89793.5 45 93859.5 46 96009.5 47 96544.5 48 96779.0 49 92946.5 50 88070.0 51 83573.5 52 79898.0 53 79013.0 54 78485.5 55 73703.5 56 66744.0 57 60525.0 58 55623.0 59 48868.5 60 39634.5 61 32761.0 62 27651.5 63 21523.5 64 15895.5 65 12221.0 66 9340.0 67 7225.0 68 5441.0 69 4018.0 70 3118.5 71 2365.0 72 1778.0 73 1443.5 74 1111.0 75 779.5 76 561.5 77 383.5 78 274.0 79 199.0 80 130.5 81 82.0 82 51.5 83 28.0 84 16.5 85 12.5 86 10.5 87 10.5 88 9.5 89 7.5 90 4.0 91 3.5 92 3.5 93 1.0 94 1.0 95 1.5 96 1.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006685914716212591 2 0.10309759615650896 3 0.11290887929035938 4 0.11591556283137806 5 0.15607061275419334 6 0.09530395592518422 7 0.026783220490390083 8 0.027099713494707838 9 0.023183112566275612 10-11 0.05034216849929303 12-13 0.05307192066153368 14-15 0.02765357625226391 16-17 0.03131302661468797 18-19 0.036594503624240514 20-21 0.05872923311371356 22-23 0.031154780112529087 24-25 0.04943225111187948 26-27 0.057562165160291834 28-29 0.05354666016801032 30-31 0.06798665349000792 32-33 0.050797127192999815 34-35 0.06236890266336775 36-37 0.05265652359336662 38-39 0.03206469749994264 40-41 0.035743928675136545 42-43 0.032203163189331654 44-45 0.0066661339034427315 46-47 0.012165199853463739 48-49 0.008446407052730108 50-51 0.007971667546253475 52-53 0.009633255818921691 54-55 0.010582734831874961 56-57 0.01372788406228266 58-59 0.019503881391081702 60-61 0.024607331085705512 62-63 0.010424488329716082 64-65 0.008861804120897163 66-67 0.009198077937984777 68-69 0.011591556283137806 70-71 0.031134999299759228 72-73 0.027673357065033773 74-75 0.028879986643995218 76-77 0.0229457428130373 78-79 0.004391340434908862 80-81 0.004233093932749984 82-83 0.0056177508266401655 84-85 0.00451002531152802 86-87 0.012679500985480091 88-89 0.03702968150517743 90-91 0.03469554559833398 92-93 0.02935472615047185 94-95 0.046386005945321086 96-97 0.04211335038703138 98-99 0.02567549497527794 100-101 0.07352528106556865 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2527702.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.367879439839406 #Duplication Level Percentage of deduplicated Percentage of total 1 75.95330398752975 42.81326682226551 2 13.459274779451283 15.173415562315624 3 4.651190075226784 7.865331642364828 4 2.081241338906551 4.692606435067777 5 1.1659967874088386 3.286238316995074 6 0.6576620445611409 2.224260890398841 7 0.4135332592693625 1.6316995032001496 8 0.292056693776803 1.3170093203527142 9 0.20330950821924446 1.031411326344787 >10 1.020671108684091 10.33979773221254 >50 0.06427634309094196 2.4830508918457084 >100 0.03241998789245827 3.298263583257218 >500 0.0028837156289818772 1.1316577556484668 >1k 0.0019693667710043478 1.6951475146482855 >5k 1.4066905507173914E-4 0.5787138543705364 >10k+ 7.033452753586957E-5 0.4381288487119817 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11051 0.43719552383943994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8518 0.3369859263473305 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6079 0.2404951216559547 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.956162553971948E-5 2 0.0 0.0 0.0 0.0 3.956162553971948E-5 3 0.0 0.0 0.0 0.0 3.956162553971948E-5 4 0.0 0.0 0.0 0.0 3.956162553971948E-5 5 0.0 0.0 0.0 0.0 3.956162553971948E-5 6 3.956162553971948E-5 0.0 0.0 0.0 1.1868487661915842E-4 7 7.912325107943896E-5 0.0 0.0 0.0 1.1868487661915842E-4 8 7.912325107943896E-5 0.0 0.0 0.0 1.1868487661915842E-4 9 7.912325107943896E-5 0.0 0.0 3.956162553971948E-5 1.1868487661915842E-4 10-11 1.1868487661915842E-4 0.0 0.0 7.912325107943896E-5 1.1868487661915842E-4 12-13 1.1868487661915842E-4 0.0 0.0 1.1868487661915842E-4 1.1868487661915842E-4 14-15 1.9780812769859738E-4 0.0 0.0 1.9780812769859738E-4 1.5824650215887792E-4 16-17 2.1758894046845713E-4 0.0 0.0 1.9780812769859738E-4 1.5824650215887792E-4 18-19 2.5715056600817657E-4 0.0 0.0 2.3736975323831685E-4 1.5824650215887792E-4 20-21 2.7693137877803634E-4 0.0 0.0 2.7693137877803634E-4 1.5824650215887792E-4 22-23 2.7693137877803634E-4 0.0 0.0 5.538627575560727E-4 1.5824650215887792E-4 24-25 2.7693137877803634E-4 0.0 0.0 7.516708852546701E-4 1.5824650215887792E-4 26-27 2.967121915478961E-4 0.0 0.0 0.0016022458343586388 1.5824650215887792E-4 28-29 3.3627381708761556E-4 0.0 0.0 0.0037187928007336307 1.5824650215887792E-4 30-31 3.560546298574753E-4 0.0 0.0 0.009910187197699729 1.5824650215887792E-4 32-33 3.75835442627335E-4 0.0 0.0 0.023242455004585192 1.5824650215887792E-4 34-35 3.9561625539719477E-4 0.0 0.0 0.04504091067697062 1.9780812769859738E-4 36-37 3.9561625539719477E-4 0.0 0.0 0.08074527772656745 1.9780812769859738E-4 38-39 4.3517788093691426E-4 0.0 0.0 0.13332267806885462 1.9780812769859738E-4 40-41 4.3517788093691426E-4 0.0 0.0 0.19414867733617333 1.9780812769859738E-4 42-43 5.340819447862129E-4 0.0 0.0 0.25558788179935765 1.9780812769859738E-4 44-45 6.725476341752311E-4 0.0 0.0 0.33516609157250343 1.9780812769859738E-4 46-47 7.912325107943895E-4 0.0 0.0 0.43167667707664903 1.9780812769859738E-4 48-49 7.912325107943895E-4 0.0 0.0 0.5359413411865798 1.9780812769859738E-4 50-51 7.912325107943895E-4 3.956162553971948E-5 0.0 0.6515008493881003 1.9780812769859738E-4 52-53 7.912325107943895E-4 3.956162553971948E-5 0.0 0.7653394268786431 1.9780812769859738E-4 54-55 7.912325107943895E-4 3.956162553971948E-5 0.0 0.8873672608559078 1.9780812769859738E-4 56-57 7.912325107943895E-4 3.956162553971948E-5 0.0 1.0337848369784095 1.9780812769859738E-4 58-59 7.912325107943895E-4 3.956162553971948E-5 0.0 1.1959281592529498 1.9780812769859738E-4 60-61 8.110133235642493E-4 3.956162553971948E-5 0.0 1.3718784888408524 1.9780812769859738E-4 62-63 8.30794136334109E-4 3.956162553971948E-5 0.0 1.5484024619990806 1.9780812769859738E-4 64-65 8.30794136334109E-4 3.956162553971948E-5 0.0 1.7341838555336033 1.9780812769859738E-4 66-67 9.09917387413548E-4 3.956162553971948E-5 0.0 1.949458440907987 1.9780812769859738E-4 68-69 9.494790129532674E-4 3.956162553971948E-5 0.0 2.195373505262883 2.3736975323831685E-4 70-71 9.890406384929868E-4 3.956162553971948E-5 0.0 2.4456007868016085 2.3736975323831685E-4 72-73 0.0010286022640327063 3.956162553971948E-5 0.0 2.714085758526915 2.967121915478961E-4 74-75 0.0010879447023422857 3.956162553971948E-5 0.0 2.9821355523712842 3.1649300431775584E-4 76-77 0.0011077255151121454 3.956162553971948E-5 0.0 3.2782147579105447 3.1649300431775584E-4 78-79 0.0011670679534217245 3.956162553971948E-5 0.0 3.6050531273069373 3.1649300431775584E-4 80-81 0.0013055336428107428 3.956162553971948E-5 0.0 3.9532152128692384 3.1649300431775584E-4 82-83 0.0013055336428107428 3.956162553971948E-5 0.0 4.31819098928592 3.1649300431775584E-4 84-85 0.0013450952683504622 3.956162553971948E-5 0.0 4.6976067590246 3.1649300431775584E-4 86-87 0.0013846568938901817 3.956162553971948E-5 0.0 5.087387674654686 3.1649300431775584E-4 88-89 0.0013846568938901817 3.956162553971948E-5 0.0 5.4985318680762205 3.1649300431775584E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6740 0.0 45.295647 1 GTATCAA 11070 0.0 38.70423 1 TATCAAC 13975 0.0 30.522022 2 ATCAACG 14065 0.0 30.259098 3 TCAACGC 14220 0.0 29.929272 4 CAACGCA 14445 0.0 29.430162 5 AACGCAG 14880 0.0 28.516941 6 ACGCAGA 16480 0.0 25.634495 7 CGCAGAG 16620 0.0 25.389465 8 TAAGGTG 1570 0.0 23.624773 5 GCAGAGT 17570 0.0 23.177794 9 GTACATG 10505 0.0 23.04109 1 TACATGG 10440 0.0 22.77415 2 GAGTACT 9855 0.0 22.563646 12-13 GGTAAGG 1545 0.0 21.852571 3 GTAAGGT 1580 0.0 21.368496 4 ACATGGG 10835 0.0 21.32947 3 GTACTTT 10550 0.0 21.119545 14-15 CAGAGTA 17035 0.0 20.307102 10-11 AGTACTT 10410 0.0 20.242441 12-13 >>END_MODULE