FastQCFastQC Report
Thu 26 May 2016
SRR938323_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938323_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527702
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105010.4154366297925942No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88770.35118854991608983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62880.24876350139375605No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29310.11595512445691779No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58950.046.598381
GTATCAA100900.041.637811
ATCAACG131650.031.6812783
TATCAAC134250.031.2942622
TCAACGC134000.031.1256754
CAACGCA136450.030.6010135
AACGCAG141550.029.5880226
ACGCAGA160100.025.9818447
CGCAGAG160850.025.8606998
GTACCGT1301.0051734E-525.5688936
TAAGGTG14000.024.0907765
CGTAATA1000.001649031523.7627542
GCAGAGT173000.023.6053039
GTAAGGT13400.022.3339144
GTACATG101750.022.1863561
TACATGG102350.021.8705582
AGAGTAC156050.021.6200910-11
AGTACTT106650.021.57198512-13
GAGTACT102900.021.36598212-13
TGGTATC30500.021.0358832
Produced by FastQC (version 0.11.5)