##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938323_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2527702 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.755759974870454 31.0 31.0 34.0 30.0 34.0 2 31.99083080204866 31.0 31.0 34.0 30.0 34.0 3 32.04383665479554 33.0 31.0 34.0 30.0 34.0 4 35.725672171798735 37.0 35.0 37.0 33.0 37.0 5 35.62655012339271 37.0 35.0 37.0 33.0 37.0 6 35.626914881580184 37.0 35.0 37.0 33.0 37.0 7 35.56958138261552 37.0 35.0 37.0 33.0 37.0 8 35.545667962441776 37.0 35.0 37.0 33.0 37.0 9 37.12000227874963 39.0 37.0 39.0 33.0 39.0 10-11 37.01893993041901 39.0 37.0 39.0 33.0 39.0 12-13 36.91619660861922 39.0 37.0 39.0 33.0 39.0 14-15 37.92034108451075 40.0 37.0 41.0 33.0 41.0 16-17 37.83471311096007 40.0 37.0 41.0 32.5 41.0 18-19 38.10216789795633 40.0 37.0 41.0 33.0 41.0 20-21 38.082357611775436 40.0 37.0 41.0 33.0 41.0 22-23 37.97085890662744 40.0 37.0 41.0 33.0 41.0 24-25 37.843091274208746 40.0 37.0 41.0 32.0 41.0 26-27 37.639494687269305 40.0 37.0 41.0 32.0 41.0 28-29 37.519312403123465 39.5 36.5 41.0 32.0 41.0 30-31 37.250744747600784 39.0 36.0 41.0 31.0 41.0 32-33 37.028389818103555 39.0 36.0 40.5 31.0 41.0 34-35 36.910868646699655 39.0 35.5 41.0 30.5 41.0 36-37 36.845628756870866 39.0 35.0 41.0 30.0 41.0 38-39 36.67191464816659 39.0 35.0 41.0 30.0 41.0 40-41 36.49313071714941 39.0 35.0 40.0 30.0 41.0 42-43 36.26272816178489 39.0 35.0 40.0 30.0 41.0 44-45 35.93612577748485 38.0 35.0 40.0 29.0 41.0 46-47 35.51820705130589 38.0 34.5 40.0 27.5 41.0 48-49 35.1334601547176 38.0 34.0 40.0 26.5 41.0 50-51 34.74948154489731 38.0 34.0 40.0 25.5 41.0 52-53 34.25500771056082 38.0 33.0 40.0 24.0 41.0 54-55 33.92146364563544 37.5 33.0 40.0 23.0 41.0 56-57 33.58118579642695 37.0 32.5 40.0 23.0 41.0 58-59 33.101557659882374 36.5 32.0 39.0 21.5 41.0 60-61 32.69991458645046 36.0 31.0 39.0 19.5 40.0 62-63 32.21908357868135 35.5 31.0 39.0 18.0 40.0 64-65 31.690793851490405 35.0 30.0 38.5 15.0 40.0 66-67 31.18496088542083 35.0 29.5 38.0 12.5 40.0 68-69 30.673806485099906 34.0 29.0 38.0 8.0 40.0 70-71 30.082503989789934 34.0 28.5 37.0 7.5 39.0 72-73 29.569148776240237 34.0 28.0 36.5 6.5 39.0 74-75 28.965481492675956 33.5 27.0 36.0 2.0 39.0 76-77 27.769215477140897 31.5 26.0 34.5 2.0 37.0 78-79 28.43978522784727 33.0 27.0 35.0 2.0 37.0 80-81 28.045044866839525 33.0 26.0 35.0 2.0 37.0 82-83 27.567129748680816 33.0 26.0 35.0 2.0 36.0 84-85 26.98027754062781 32.0 25.5 35.0 2.0 36.0 86-87 26.337246044035254 32.0 24.5 34.5 2.0 35.5 88-89 25.54902911814763 31.0 21.5 34.0 2.0 35.0 90-91 24.894595169842013 31.0 19.0 34.0 2.0 35.0 92-93 24.02737466679221 31.0 14.0 34.0 2.0 35.0 94-95 23.17607791583027 30.0 4.5 34.0 2.0 35.0 96-97 22.067193640706066 29.5 2.0 34.0 2.0 35.0 98-99 20.659921145767974 29.0 2.0 34.0 2.0 35.0 100-101 16.771480182394917 21.0 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30147481158187617 1101 2 0.34455606794788096 1101 3 0.31631993035772865 1101 4 0.2707167122850649 1101 5 0.17857337009434815 1101 6 0.257792754040274 1101 7 0.30763167767120336 1101 8 0.2653520588573457 1101 9 0.3602984791260724 1101 10-11 0.26702107505912664 1101 12-13 0.28827261220311584 1101 14-15 0.4433245801055108 1101 16-17 0.5273417763552786 1101 18-19 0.47758485966527076 1101 20-21 0.4472785614578427 1101 22-23 0.4090696665277562 1101 24-25 0.5788526239070677 1101 26-27 0.505386001608926 1101 28-29 0.5465641583390166 1101 30-31 0.6090576145232589 1101 32-33 0.4998943213795357 1101 34-35 0.49163981876883867 1101 36-37 0.6069387352213127 1101 38-39 0.6257974863951574 1101 40-41 0.6062905206328963 1101 42-43 0.5514445182134651 1101 44-45 0.7177948718441982 1101 46-47 0.5321917037879018 1101 48-49 0.7787432273616375 1101 50-51 0.737724861147818 1101 52-53 0.7877161044578003 1101 54-55 0.7030229349986783 1101 56-57 0.7502243527240395 1101 58-59 0.9301909157144408 1101 60-61 0.7277917553989539 1101 62-63 0.9886695497255289 1101 64-65 0.9237738473542016 1101 66-67 0.7632004708812907 1101 68-69 0.9839188777719876 1101 70-71 0.9175365767670662 1101 72-73 0.870304717814065 1101 74-75 0.8004331017958854 1101 76-77 0.7914845821792653 1101 78-79 1.0978846078098918 1101 80-81 1.1490377441677992 1101 82-83 1.0793064460768065 1101 84-85 1.088226170776391 1101 86-87 1.1156642541181512 1101 88-89 1.2700755657808607 1101 90-91 1.431976627535132 1101 92-93 1.7556441647189978 1101 94-95 1.7692794214751935 1101 96-97 1.697984273847414 1101 98-99 1.7783258379614253 1101 100-101 1.838159476360186 1102 1 0.29861652355744894 1102 2 0.2835696296533037 1102 3 0.25627406040261036 1102 4 0.18139384768166167 1102 5 0.12473923038384527 1102 6 0.20610722412740756 1102 7 0.20336096471013576 1102 8 0.20505492141338522 1102 9 0.21464147561442104 1102 10-11 0.28831174276637483 1102 12-13 0.3807192323023969 1102 14-15 0.5096320005832027 1102 16-17 0.36696332194877357 1102 18-19 0.38426161126120917 1102 20-21 0.42719962559971947 1102 22-23 0.43027128349663 1102 24-25 0.45069737974919377 1102 26-27 0.5388000047492909 1102 28-29 0.5039327619688834 1102 30-31 0.44029924495723805 1102 32-33 0.4506941895523511 1102 34-35 0.4217231959322518 1102 36-37 0.5394268032246359 1102 38-39 0.5989385000845857 1102 40-41 0.487929894986479 1102 42-43 0.3836483779773303 1102 44-45 0.6142430630873221 1102 46-47 0.7296300269255909 1102 48-49 0.6277010609545286 1102 50-51 0.700329247065163 1102 52-53 0.48564597162619094 1102 54-55 0.348116191178228 1102 56-57 0.6088656919701307 1102 58-59 0.7125546817231196 1102 60-61 0.6721247749955666 1102 62-63 0.6530111631195297 1102 64-65 0.6712395917012479 1102 66-67 0.6883692099966474 1102 68-69 0.6295588733948598 1102 70-71 0.6149123237102181 1102 72-73 0.7016106091862824 1102 74-75 0.8688823128705216 1102 76-77 0.9967819939957714 1102 78-79 1.1750590262764788 1102 80-81 1.208642211273741 1102 82-83 1.15140304703419 1102 84-85 1.1256045362926095 1102 86-87 1.0017700444699287 1102 88-89 1.094342196848622 1102 90-91 1.1817531984302683 1102 92-93 1.5562355739381069 1102 94-95 1.4791664762576033 1102 96-97 1.4640716507528175 1102 98-99 1.7325338624117066 1102 100-101 1.984399882547077 1103 1 0.25186017717637554 1103 2 0.14996573314214956 1103 3 0.17252632136750634 1103 4 0.18509825521478263 1103 5 0.12006395637120448 1103 6 0.1704090657632804 1103 7 0.23969241275623432 1103 8 0.11031751320040684 1103 9 0.1853508478626935 1103 10-11 0.1939413885631751 1103 12-13 0.3052929259601598 1103 14-15 0.4502221688667234 1103 16-17 0.3424344399253627 1103 18-19 0.3445124529296919 1103 20-21 0.3757639285702723 1103 22-23 0.26611598123604097 1103 24-25 0.40343443298127113 1103 26-27 0.32459827587943835 1103 28-29 0.36582402617752763 1103 30-31 0.12343356006989126 1103 32-33 0.17409516054426177 1103 34-35 0.3486554585528907 1103 36-37 0.3219799155165006 1103 38-39 0.365185069438823 1103 40-41 0.4641288492353368 1103 42-43 0.4915357587200546 1103 44-45 0.3689057152085482 1103 46-47 0.44106630722754403 1103 48-49 0.5586466930588472 1103 50-51 0.4970362818332177 1103 52-53 0.37569510763028546 1103 54-55 0.2752644370959487 1103 56-57 0.4292188603314173 1103 58-59 0.6055781154796662 1103 60-61 0.5856400454458566 1103 62-63 0.5232468302954629 1103 64-65 0.6256962796416836 1103 66-67 0.7065421465290775 1103 68-69 0.5533152015878677 1103 70-71 0.18046368555742376 1103 72-73 0.411332428971388 1103 74-75 0.415642108712607 1103 76-77 0.3418921791879228 1103 78-79 0.6777012350102645 1103 80-81 0.7189098455455785 1103 82-83 0.7828812132003726 1103 84-85 0.8543151685349066 1103 86-87 0.7081058262934015 1103 88-89 0.6139718250845299 1103 90-91 0.7987502137057376 1103 92-93 1.111051742753478 1103 94-95 1.142226731442456 1103 96-97 1.21234488193193 1103 98-99 1.3782916211902716 1103 100-101 1.3956754336455113 1104 1 0.23568606724395735 1104 2 0.21616566945901283 1104 3 0.21369636132508418 1104 4 0.1913310027111521 1104 5 0.15805986484944157 1104 6 0.10639494534469662 1104 7 0.22632688472487672 1104 8 0.2100607755964745 1104 9 0.2471537150402625 1104 10-11 0.2833437726107846 1104 12-13 0.3826982361999782 1104 14-15 0.44402810109446733 1104 16-17 0.4276771450939876 1104 18-19 0.41751171614454563 1104 20-21 0.40340010514014324 1104 22-23 0.46533511466657274 1104 24-25 0.4538102472755057 1104 26-27 0.304702907151686 1104 28-29 0.4210426616346652 1104 30-31 0.5008064900687259 1104 32-33 0.43035805877334354 1104 34-35 0.5331480164841977 1104 36-37 0.6265502728124162 1104 38-39 0.40956672354842283 1104 40-41 0.55472087795021 1104 42-43 0.7494840305369124 1104 44-45 0.7271805875430104 1104 46-47 0.4998876185734389 1104 48-49 0.5769297831513356 1104 50-51 0.6612776475013007 1104 52-53 0.6065957780987858 1104 54-55 0.44298667867050767 1104 56-57 0.4779066824878839 1104 58-59 0.7641209926645942 1104 60-61 0.6895523386851465 1104 62-63 0.7270298719073622 1104 64-65 0.6498405823951394 1104 66-67 0.7359400087149375 1104 68-69 0.8648321979224995 1104 70-71 1.0078126595646495 1104 72-73 0.8581747419859695 1104 74-75 1.0848858774614207 1104 76-77 0.934042020698314 1104 78-79 1.2762186419335286 1104 80-81 1.225041591828134 1104 82-83 1.2867712562463396 1104 84-85 1.5204005919200263 1104 86-87 1.6735227114219562 1104 88-89 1.6954392545123618 1104 90-91 1.7329404413370355 1104 92-93 2.02525562574888 1104 94-95 2.096066184102664 1104 96-97 1.9899963209074478 1104 98-99 1.9203334138466026 1104 100-101 2.083651934927552 1105 1 0.24033120573687228 1105 2 0.2604035521243837 1105 3 0.2030982811612816 1105 4 0.17705151878237757 1105 5 0.10423352207121184 1105 6 0.12201882635390149 1105 7 0.136718423757209 1105 8 0.21252600388453402 1105 9 0.3292612836741995 1105 10-11 0.2296124882119841 1105 12-13 0.34151851739838435 1105 14-15 0.5541142096917255 1105 16-17 0.3929842672823085 1105 18-19 0.4241423597355123 1105 20-21 0.4670502668608876 1105 22-23 0.3924930220728484 1105 24-25 0.45416367861855633 1105 26-27 0.28881314856995033 1105 28-29 0.43105330960261057 1105 30-31 0.4412524153591164 1105 32-33 0.38419027658826366 1105 34-35 0.5423303700467628 1105 36-37 0.5030337731792827 1105 38-39 0.5633119307835486 1105 40-41 0.5920973324327576 1105 42-43 0.5598276258392261 1105 44-45 0.6082697021292418 1105 46-47 0.36091305405159346 1105 48-49 0.5658201030778116 1105 50-51 0.5610821131295509 1105 52-53 0.47259891345291294 1105 54-55 0.5672833717395847 1105 56-57 0.4245291867944587 1105 58-59 0.4827777791681811 1105 60-61 0.4755858612728048 1105 62-63 0.6061396995000479 1105 64-65 0.658385758925693 1105 66-67 0.5960143600915906 1105 68-69 0.5545990275797408 1105 70-71 0.6592585261046864 1105 72-73 0.8315701172314256 1105 74-75 0.8385495203186579 1105 76-77 0.8204345351868554 1105 78-79 0.9828210253115479 1105 80-81 0.9384415070046153 1105 82-83 1.0880519284763288 1105 84-85 1.0174813705296337 1105 86-87 0.9367878938532783 1105 88-89 1.039200087057953 1105 90-91 1.1697965905635392 1105 92-93 1.5046585476290488 1105 94-95 1.5668774008770825 1105 96-97 1.4789929203904713 1105 98-99 1.6895576131084056 1105 100-101 1.5952732149107902 1106 1 -0.06507026684733219 1106 2 0.11512608511127453 1106 3 0.07131298179564283 1106 4 0.049405705905009256 1106 5 0.008297255610251852 1106 6 -0.051981814377164426 1106 7 -0.002940591240779611 1106 8 0.012757521760249801 1106 9 -0.2873945766111987 1106 10-11 0.004799694149561162 1106 12-13 -0.060131430056756585 1106 14-15 -0.05418991320620847 1106 16-17 0.05792689384366412 1106 18-19 0.04140538416804418 1106 20-21 0.04575977559571953 1106 22-23 0.11784526895556979 1106 24-25 0.11764177703616951 1106 26-27 0.016845406881301983 1106 28-29 0.032900096339218976 1106 30-31 0.020247784279739278 1106 32-33 -0.04013870632841332 1106 34-35 -0.06894698753098538 1106 36-37 0.0781397832741888 1106 38-39 -0.01537678811958898 1106 40-41 -0.018358610232354522 1106 42-43 0.17466697458042546 1106 44-45 0.13244300674373477 1106 46-47 -0.6179802858825099 1106 48-49 -0.5232324191113591 1106 50-51 0.0825401791005973 1106 52-53 0.05460375488496538 1106 54-55 0.2538387608321031 1106 56-57 0.1631532868582326 1106 58-59 0.08181548427106833 1106 60-61 0.11884888021913298 1106 62-63 -0.05453711347072243 1106 64-65 0.06967215430526963 1106 66-67 -0.0012532496538177895 1106 68-69 -0.09980827958410288 1106 70-71 -0.05256805840172163 1106 72-73 0.27656137756155275 1106 74-75 0.34635408088586317 1106 76-77 0.2413165206966319 1106 78-79 0.4533755493160818 1106 80-81 -0.14923094573208928 1106 82-83 0.3926165381479585 1106 84-85 0.6825830970529658 1106 86-87 0.7703342507745532 1106 88-89 0.7383788020817654 1106 90-91 0.9256115714563826 1106 92-93 0.984631298512145 1106 94-95 1.2625510080269322 1106 96-97 1.490304465691441 1106 98-99 1.4309858860956446 1106 100-101 1.5541266008308483 1107 1 0.07187337912059633 1107 2 0.14363790470438786 1107 3 0.11007184531310088 1107 4 0.06903495591934927 1107 5 0.044940691533902566 1107 6 0.10560748012923682 1107 7 0.08331328233363422 1107 8 0.087172964167614 1107 9 0.024495487943376304 1107 10-11 0.13852824124768404 1107 12-13 0.12663201281984726 1107 14-15 0.22007542417902215 1107 16-17 0.22186033044376785 1107 18-19 0.14388973788157955 1107 20-21 0.17691971151209174 1107 22-23 0.21336510927738317 1107 24-25 0.11169772947523171 1107 26-27 0.19543677442966612 1107 28-29 0.20334293612415877 1107 30-31 0.09085023374024104 1107 32-33 0.3056230317044921 1107 34-35 0.2177075799687529 1107 36-37 0.300667267563675 1107 38-39 0.3424821902243522 1107 40-41 0.2562205519709835 1107 42-43 0.25058539382812484 1107 44-45 0.3990316239365441 1107 46-47 0.42728305723804993 1107 48-49 0.5251047374902171 1107 50-51 0.4684109123888831 1107 52-53 0.45203570028201767 1107 54-55 0.3844269190614611 1107 56-57 0.4373982877102236 1107 58-59 0.4173366004427592 1107 60-61 0.37227719158472894 1107 62-63 0.6100224742842002 1107 64-65 0.3625884474219312 1107 66-67 0.35482895789185065 1107 68-69 0.7274114637217544 1107 70-71 0.6984123765688963 1107 72-73 0.8263878106021281 1107 74-75 0.6625473558726114 1107 76-77 0.759704385908659 1107 78-79 1.030814284974543 1107 80-81 0.9256419616386431 1107 82-83 0.9953138902885179 1107 84-85 1.0563771406322324 1107 86-87 1.1893762245228707 1107 88-89 1.2700716836101158 1107 90-91 1.1580343846796666 1107 92-93 1.2674816498846262 1107 94-95 1.12813444389921 1107 96-97 1.2489592660980016 1107 98-99 1.0672571420542454 1107 100-101 1.1846426178844212 1108 1 0.11577742620855602 1108 2 0.15107240718264947 1108 3 0.1227180590791761 1108 4 0.1426062215920041 1108 5 0.15882118955443048 1108 6 0.18176609113837117 1108 7 0.13230328365902722 1108 8 0.08824882279860446 1108 9 0.20718416802846207 1108 10-11 0.24775184265266148 1108 12-13 0.30409146041010615 1108 14-15 0.34239125297874295 1108 16-17 0.3167140693421757 1108 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-0.9074228780184619 2316 76-77 -0.9163527592685803 2316 78-79 -0.9114834629572393 2316 80-81 -0.9149581566137606 2316 82-83 -1.2345092583663693 2316 84-85 -1.3147541040264628 2316 86-87 -1.5625467163021511 2316 88-89 -1.4729722471290536 2316 90-91 -1.3255892506878908 2316 92-93 -1.711093913372089 2316 94-95 -1.6409720314771405 2316 96-97 -1.973657436250214 2316 98-99 -1.838310462426712 2316 100-101 -2.1880946489061373 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 17.0 4 54.0 5 96.0 6 217.0 7 523.0 8 1245.0 9 2300.0 10 3853.0 11 5885.0 12 8116.0 13 10996.0 14 14722.0 15 19658.0 16 24764.0 17 30871.0 18 29408.0 19 24791.0 20 24188.0 21 25688.0 22 28033.0 23 31107.0 24 35971.0 25 41976.0 26 49429.0 27 59205.0 28 70247.0 29 82407.0 30 97983.0 31 114276.0 32 138251.0 33 168792.0 34 211016.0 35 271453.0 36 346457.0 37 376091.0 38 171697.0 39 5913.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.033447877789 20.287147099292874 16.322426686141597 22.356978336776525 2 15.54170819423741 21.358127700941328 39.471959291238164 23.628204813583096 3 18.79481837653331 24.937670658962173 30.729492637977103 25.538018326527418 4 13.108211503114692 18.187417958949208 37.26548820310021 31.43888233483589 5 13.409543003198731 37.85506837972854 34.436794365291895 14.298594251780832 6 30.962114996150653 37.35175269869629 17.458664035554825 14.227468269598237 7 27.76011570984238 33.30815895228156 20.828641983904745 18.103083353971314 8 26.10936732257204 35.18967030132508 19.964655643742816 18.736306732360063 9 26.16748335048989 16.774247913717677 20.41672633878519 36.64154239700724 10-11 24.48898248290344 27.200239585204265 27.551289669430968 20.759488262461318 12-13 25.62750672349826 24.437097411008104 27.860898950904815 22.074496914588824 14-15 22.22038040876654 25.93175540471147 26.57109105424611 25.276773132275878 16-17 21.474782233032215 29.07595515610622 27.339001986784833 22.11026062407673 18-19 21.47401078133419 27.365725864836914 29.97845473873107 21.181808615097825 20-21 22.187484917130263 26.446056536727824 30.169497828462372 21.196960717679538 22-23 21.947405192542472 26.442871825871876 30.073936721971183 21.535786259614465 24-25 21.81068688880475 26.62317751699586 30.11516790918554 21.45096768501385 26-27 21.40254081301823 26.930311508035803 29.91339207419682 21.753755604749145 28-29 21.47432448470942 27.212525220556238 29.853503184713375 21.459647110020967 30-31 21.68478055533082 26.809768464129636 29.78907999855202 21.716370981987524 32-33 21.352617060817327 26.83320854890379 29.724837969808434 22.08933642047045 34-35 22.081055036606067 26.97989994783138 29.49835630275841 21.44068871280414 36-37 22.13487276609586 26.539485524388244 29.524039226331755 21.801602483184134 38-39 21.59573965124438 26.874550089581504 29.5387912540283 21.990919005145816 40-41 22.09283464619886 26.697734718478667 29.506044482740478 21.703386152581995 42-43 21.80933249857329 26.74011368165773 29.285122973802974 22.165430845966004 44-45 21.834262847954726 26.987233153602553 29.325012542116983 21.853491456325738 46-47 22.154648767932294 27.288541133408923 28.620976681586676 21.935833417072107 48-49 22.036003452938676 27.216914810369257 28.55987375094058 22.187207985751485 50-51 22.164901550427466 27.39637772036919 28.47805704022249 21.960663688980848 52-53 22.377736774350772 27.28759165439597 28.393477553920516 21.941194017332737 54-55 21.941559095362535 27.43754165159182 28.470174740358146 22.150724512687503 56-57 22.084763156416383 27.46991140569577 28.332809801155356 22.112515636732493 58-59 22.10760807541358 27.24239220158618 28.249523758269433 22.400475964730806 60-61 21.813390186026677 27.481265592225668 28.538925870217298 22.16641835153036 62-63 21.84093140959343 27.549063518076405 28.53723810435706 22.072766967973102 64-65 21.637853126122746 27.415062968783882 28.445497221523187 22.50158668357019 66-67 21.58396102421427 27.462712431721553 28.343853535365525 22.609473008698654 68-69 22.007321812742187 27.273475482967015 28.371153862133557 22.348048842157244 70-71 21.871595841038793 27.31261263952168 28.107070522849142 22.708720996590376 72-73 21.830214323425047 27.34145310447429 28.073482656258253 22.754849915842414 74-75 21.889202839524224 27.667435594008737 27.795922106387387 22.647439460079653 76-77 21.859079478961707 27.30600292825769 28.210960423398035 22.62395716938257 78-79 21.854520198822264 27.473623599062876 28.09864338631039 22.57321281580447 80-81 21.998422643650827 27.632168888723562 27.81691269494854 22.55249577267707 82-83 22.06891570461193 27.567706622445787 27.85408693518449 22.509290737757794 84-85 21.9561979214994 27.548867753524625 27.865594857977307 22.629339466998672 86-87 22.033984813780677 27.636925041831145 27.64693523798747 22.682154906400708 88-89 21.889192543042384 27.684976721754683 27.41964669650062 23.006184038702308 90-91 21.83768245671207 27.61054743295765 27.65910296523135 22.892667145098926 92-93 21.87057044199223 27.398658835689567 27.694259490680466 23.03651123163774 94-95 21.633746489374037 27.247166667128354 27.809222978815352 23.309863864682264 96-97 21.84472484430591 26.983703250464693 27.836941079947337 23.33463082528206 98-99 21.681376083365215 27.21117732267307 27.401908579225314 23.705538014736398 100-101 21.709266266043475 26.940930336078345 27.870949872641393 23.478853525236786 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1009.0 1 690.5 2 455.0 3 552.0 4 513.5 5 506.5 6 893.5 7 2123.0 8 3326.5 9 3664.5 10 3101.5 11 2327.0 12 2214.5 13 2338.5 14 2487.0 15 2763.5 16 3079.0 17 3355.0 18 3716.5 19 4128.5 20 4693.5 21 5627.5 22 6848.0 23 8290.5 24 10074.5 25 12455.0 26 15643.5 27 19627.0 28 23625.5 29 27758.5 30 32466.0 31 38589.0 32 46780.5 33 51757.5 34 53871.5 35 57534.0 36 62262.5 37 66719.5 38 70160.0 39 73156.0 40 76755.5 41 81843.5 42 85793.5 43 88448.0 44 91299.0 45 95177.0 46 96683.5 47 97581.5 48 98090.0 49 93662.5 50 88720.0 51 84310.0 52 80224.5 53 78838.0 54 78725.5 55 74004.5 56 66534.0 57 60323.0 58 54916.0 59 47954.5 60 38623.0 61 31411.0 62 26579.5 63 20539.0 64 14717.0 65 11062.5 66 8426.5 67 6538.5 68 4935.5 69 3629.0 70 2716.5 71 1990.0 72 1522.5 73 1303.5 74 985.0 75 672.5 76 496.5 77 364.0 78 249.5 79 157.5 80 97.5 81 49.5 82 25.5 83 17.0 84 9.5 85 5.5 86 4.5 87 2.5 88 2.5 89 1.5 90 1.0 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.1649300431775584E-4 2 0.0501641411843643 3 0.0 4 1.5824650215887792E-4 5 0.04256830908073816 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006923284469450909 26-27 0.0018396155875969556 28-29 7.912325107943896E-5 30-31 0.0014835609577394803 32-33 3.75835442627335E-4 34-35 0.012679500985480091 36-37 0.024686454336784954 38-39 0.0045693677498375995 40-41 9.89040638492987E-4 42-43 0.0011670679534217245 44-45 0.008624434367658846 46-47 0.0 48-49 0.0 50-51 4.7473950647663375E-4 52-53 0.0 54-55 0.0014835609577394803 56-57 0.0 58-59 0.024686454336784954 60-61 0.0 62-63 0.01044426914248594 64-65 0.016536759475602743 66-67 0.007279339099308384 68-69 0.01815878612273124 70-71 1.3846568938901817E-4 72-73 1.3846568938901817E-4 74-75 0.023282016630124913 76-77 0.050836688818539526 78-79 0.03251965619364941 80-81 0.02725795999686672 82-83 0.03924513253540172 84-85 0.017703827429024464 86-87 0.011037693525581734 88-89 0.08576960417011183 90-91 0.027633795439494055 92-93 0.02547768684757934 94-95 0.028919548269534936 96-97 0.04084737836976036 98-99 0.05429833105326499 100-101 0.015409253147720736 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2527702.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.236104471102855 #Duplication Level Percentage of deduplicated Percentage of total 1 74.15310414089505 40.217755030421536 2 14.424315732612989 15.64637389996341 3 4.953620618758723 8.059952561676218 4 2.2832990024696356 4.953489729468323 5 1.2298371391149376 3.335078777974 6 0.710932395609011 2.3134922208085045 7 0.46119495679814565 1.7509392500914995 8 0.3184383130058422 1.3816682889429286 9 0.23383991738294505 1.1414309569825922 >10 1.114399118178081 10.87132806212378 >50 0.07446899629469275 2.7478123674142734 >100 0.03714945176222332 3.6347424629209923 >500 0.0030649880939261505 1.1701221046757395 >1k 0.0021163013029467746 1.7599722510003222 >5k 1.4595181399632926E-4 0.6002199200850042 >10k+ 7.297590699816463E-5 0.41562211545088223 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10501 0.4154366297925942 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8877 0.35118854991608983 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6288 0.24876350139375605 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2931 0.11595512445691779 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.956162553971948E-5 0.0 3 0.0 0.0 0.0 3.956162553971948E-5 0.0 4 0.0 0.0 0.0 3.956162553971948E-5 0.0 5 1.1868487661915842E-4 0.0 0.0 3.956162553971948E-5 0.0 6 1.1868487661915842E-4 0.0 0.0 3.956162553971948E-5 0.0 7 1.5824650215887792E-4 0.0 0.0 3.956162553971948E-5 0.0 8 2.3736975323831685E-4 0.0 0.0 3.956162553971948E-5 0.0 9 2.3736975323831685E-4 0.0 0.0 2.3736975323831685E-4 3.956162553971948E-5 10-11 2.3736975323831685E-4 0.0 0.0 2.3736975323831685E-4 3.956162553971948E-5 12-13 2.5715056600817657E-4 0.0 0.0 2.7693137877803634E-4 3.956162553971948E-5 14-15 3.1649300431775584E-4 0.0 0.0 2.7693137877803634E-4 3.956162553971948E-5 16-17 3.1649300431775584E-4 0.0 0.0 3.1649300431775584E-4 3.956162553971948E-5 18-19 3.560546298574753E-4 0.0 0.0 3.560546298574753E-4 3.956162553971948E-5 20-21 3.9561625539719477E-4 0.0 0.0 3.75835442627335E-4 3.956162553971948E-5 22-23 3.9561625539719477E-4 0.0 0.0 6.329860086355117E-4 3.956162553971948E-5 24-25 3.9561625539719477E-4 0.0 0.0 8.703557618738285E-4 3.956162553971948E-5 26-27 3.9561625539719477E-4 0.0 0.0 0.0017209307109777973 3.956162553971948E-5 28-29 5.538627575560727E-4 0.0 0.0 0.0038770393028925085 3.956162553971948E-5 30-31 5.538627575560727E-4 0.0 0.0 0.010147556950938046 3.956162553971948E-5 32-33 5.538627575560727E-4 0.0 0.0 0.023618290447212528 3.956162553971948E-5 34-35 5.538627575560727E-4 0.0 0.0 0.04551565018344726 3.956162553971948E-5 36-37 5.538627575560727E-4 0.0 0.0 0.08135848292243311 3.956162553971948E-5 38-39 5.934243830957921E-4 0.0 0.0 0.1342325954562682 3.956162553971948E-5 40-41 5.934243830957921E-4 0.0 0.0 0.19535530691513478 3.956162553971948E-5 42-43 6.329860086355117E-4 0.0 0.0 0.25740771657418476 3.956162553971948E-5 44-45 7.714516980245298E-4 0.0 0.0 0.3372232961005688 3.956162553971948E-5 46-47 8.901365746436882E-4 0.0 0.0 0.43458445655381844 3.956162553971948E-5 48-49 9.494790129532674E-4 0.0 0.0 0.5388293398509792 3.956162553971948E-5 50-51 9.494790129532674E-4 0.0 0.0 0.6536767387927849 3.956162553971948E-5 52-53 9.494790129532674E-4 0.0 0.0 0.7672977273428592 3.956162553971948E-5 54-55 9.494790129532674E-4 0.0 0.0 0.8898794240776801 3.956162553971948E-5 56-57 9.494790129532674E-4 0.0 0.0 1.036435465889571 3.956162553971948E-5 58-59 9.494790129532674E-4 0.0 0.0 1.1981040486576346 3.956162553971948E-5 60-61 9.494790129532674E-4 0.0 0.0 1.3734016114241316 3.956162553971948E-5 62-63 9.494790129532674E-4 0.0 0.0 1.5488772015055572 3.956162553971948E-5 64-65 9.494790129532674E-4 0.0 0.0 1.7336893352143568 3.956162553971948E-5 66-67 9.890406384929868E-4 0.0 0.0 1.949260632780288 7.912325107943896E-5 68-69 9.890406384929868E-4 0.0 0.0 2.194780080879787 7.912325107943896E-5 70-71 9.890406384929868E-4 0.0 0.0 2.4462139919974746 7.912325107943896E-5 72-73 0.0010088214512628466 0.0 0.0 2.714936333476019 9.890406384929869E-5 74-75 0.0010286022640327065 0.0 0.0 2.986269742240185 1.1868487661915842E-4 76-77 0.0010286022640327065 0.0 0.0 3.286364452771727 1.1868487661915842E-4 78-79 0.0010286022640327065 0.0 0.0 3.6181084637350445 1.1868487661915842E-4 80-81 0.001068163889572426 0.0 0.0 3.9705629856684057 1.1868487661915842E-4 82-83 0.001068163889572426 0.0 0.0 4.339930102519997 1.1868487661915842E-4 84-85 0.001068163889572426 0.0 0.0 4.7248844998342365 1.1868487661915842E-4 86-87 0.0010879447023422857 0.0 0.0 5.121509576682694 1.1868487661915842E-4 88-89 0.0011077255151121454 0.0 0.0 5.541297985284658 1.9780812769859738E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5895 0.0 46.59838 1 GTATCAA 10090 0.0 41.63781 1 ATCAACG 13165 0.0 31.681278 3 TATCAAC 13425 0.0 31.294262 2 TCAACGC 13400 0.0 31.125675 4 CAACGCA 13645 0.0 30.601013 5 AACGCAG 14155 0.0 29.588022 6 ACGCAGA 16010 0.0 25.981844 7 CGCAGAG 16085 0.0 25.860699 8 GTACCGT 130 1.0051734E-5 25.568893 6 TAAGGTG 1400 0.0 24.090776 5 CGTAATA 100 0.0016490315 23.762754 2 GCAGAGT 17300 0.0 23.605303 9 GTAAGGT 1340 0.0 22.333914 4 GTACATG 10175 0.0 22.186356 1 TACATGG 10235 0.0 21.870558 2 AGAGTAC 15605 0.0 21.62009 10-11 AGTACTT 10665 0.0 21.571985 12-13 GAGTACT 10290 0.0 21.365982 12-13 TGGTATC 3050 0.0 21.035883 2 >>END_MODULE