FastQCFastQC Report
Thu 26 May 2016
SRR938322_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938322_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2539522
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111810.44027970618092693No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87630.3450649374173565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62420.24579428727138417No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68650.041.8787541
GTATCAA112650.039.6763531
ATCAACG139150.031.7331453
TATCAAC141900.031.1851522
TCAACGC142050.031.08534
CAACGCA144700.030.4813615
AACGCAG148650.029.6649676
GTAAGGT14750.028.035474
TAAGGTG16050.027.5399285
ACGCAGA166100.026.4028037
CGCAGAG166500.026.28238
GGTAAGG16450.024.5602973
GCAGAGT175850.023.8827929
GAGTACT97050.022.68660412-13
AGGTAAG17150.022.1720812
GTACATG103650.021.905161
TACATGG102150.021.7764992
AGAGTAC154550.021.50147410-11
AGTACTT101100.021.1434912-13
GTACTTT103350.021.12086514-15
Produced by FastQC (version 0.11.5)