##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938322_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2539522 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.207357526337635 31.0 31.0 34.0 28.0 34.0 2 31.327241898278494 31.0 31.0 34.0 28.0 34.0 3 31.381991965417114 31.0 31.0 34.0 28.0 34.0 4 35.015633256967256 37.0 35.0 37.0 32.0 37.0 5 35.04103134369382 37.0 35.0 37.0 32.0 37.0 6 35.07680736768573 37.0 35.0 37.0 32.0 37.0 7 35.01325525039751 37.0 35.0 37.0 32.0 37.0 8 34.97656369978287 37.0 35.0 37.0 32.0 37.0 9 36.50275681801536 39.0 37.0 39.0 32.0 39.0 10-11 36.36301319697171 39.0 37.0 39.0 32.0 39.0 12-13 36.22521108303059 39.0 35.0 39.0 32.0 39.0 14-15 37.23244394023757 39.5 36.5 41.0 31.5 41.0 16-17 37.10068489266878 39.0 36.0 41.0 31.0 41.0 18-19 37.12305977266588 39.5 36.5 41.0 31.0 41.0 20-21 37.2174269409755 40.0 37.0 41.0 31.0 41.0 22-23 37.14225708617606 39.5 36.0 41.0 31.0 41.0 24-25 37.03536984519134 39.0 36.0 41.0 31.0 41.0 26-27 36.85730090150824 39.0 36.0 41.0 30.0 41.0 28-29 36.67831761252708 39.0 36.0 41.0 30.0 41.0 30-31 36.45280332283004 39.0 35.5 40.5 30.0 41.0 32-33 36.161570366391786 39.0 35.0 40.0 29.5 41.0 34-35 35.91275681013986 38.0 35.0 40.0 28.5 41.0 36-37 35.626159371724285 38.0 35.0 40.0 27.0 41.0 38-39 35.37342814907687 38.0 34.5 40.0 26.5 41.0 40-41 35.29773417989685 38.0 34.0 40.0 26.0 41.0 42-43 35.10697426523574 38.0 34.0 40.0 25.5 41.0 44-45 34.79572179331386 38.0 34.0 40.0 24.5 41.0 46-47 34.41735905418422 38.0 33.0 40.0 23.5 41.0 48-49 34.0358667891044 38.0 33.0 40.0 22.5 41.0 50-51 33.29452904916752 37.5 32.0 39.5 20.5 40.5 52-53 33.35617883995492 37.5 33.0 39.5 20.0 40.5 54-55 33.50074659719428 38.0 33.0 40.0 19.5 41.0 56-57 33.21862716684478 37.5 32.5 40.0 18.0 41.0 58-59 32.915371081644494 37.0 32.0 40.0 16.0 41.0 60-61 32.48790303844582 36.5 31.0 39.5 12.5 41.0 62-63 32.000506985172805 36.0 31.0 39.0 8.0 41.0 64-65 31.52869457323071 35.5 30.5 39.0 7.5 40.0 66-67 31.016248727122665 35.0 29.5 38.5 4.0 40.0 68-69 30.47722327272613 35.0 29.0 38.0 2.0 40.0 70-71 29.875334413326602 34.0 28.5 37.5 2.0 39.5 72-73 29.34206673539351 34.0 28.0 37.0 2.0 39.0 74-75 28.750023429606046 34.0 26.5 36.0 2.0 39.0 76-77 28.0328575613836 33.5 26.0 36.0 2.0 38.5 78-79 27.27286394841234 33.0 25.0 35.0 2.0 37.5 80-81 26.648755750097852 32.0 23.5 35.0 2.0 37.0 82-83 25.999874779584502 32.0 21.5 35.0 2.0 36.0 84-85 25.35787856927406 31.5 19.5 35.0 2.0 36.0 86-87 24.712589810208378 31.0 17.5 34.0 2.0 35.5 88-89 23.95059739588789 31.0 9.0 34.0 2.0 35.0 90-91 23.1527029496102 30.0 3.5 34.0 2.0 35.0 92-93 22.342309694501566 29.5 2.0 34.0 2.0 35.0 94-95 21.412160831841582 29.0 2.0 34.0 2.0 35.0 96-97 20.210490793149262 28.0 2.0 34.0 2.0 35.0 98-99 18.764647047751506 25.5 2.0 33.5 2.0 35.0 100-101 15.241966007776266 10.5 2.0 30.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6059048941114007 1101 2 0.5185937785116828 1101 3 0.5632562538636243 1101 4 0.45588417978065365 1101 5 0.4808280206456743 1101 6 0.46606613392027896 1101 7 0.3477392473598613 1101 8 0.5183871580177808 1101 9 0.4492261346043236 1101 10-11 0.5672387721155729 1101 12-13 0.6121447237084752 1101 14-15 0.8094542610229141 1101 16-17 0.7350417442323973 1101 18-19 0.6493131138149337 1101 20-21 0.8409558153604806 1101 22-23 0.8567366650899757 1101 24-25 0.8065836341514938 1101 26-27 0.8241739021313634 1101 28-29 0.8405798225624252 1101 30-31 0.8008692016286147 1101 32-33 0.7625282854911788 1101 34-35 0.7902852141075911 1101 36-37 0.8398917138890525 1101 38-39 0.9433831831707735 1101 40-41 0.9349862569993093 1101 42-43 0.9545026195463677 1101 44-45 0.9600439770471709 1101 46-47 1.3090666026961912 1101 48-49 1.2511765040314984 1101 50-51 1.136606038108873 1101 52-53 1.1280458973051495 1101 54-55 1.037545834095127 1101 56-57 0.9765055369850657 1101 58-59 0.9472526352335819 1101 60-61 0.8847724712556513 1101 62-63 0.8828811504091405 1101 64-65 0.8373669789426437 1101 66-67 0.808229286643968 1101 68-69 0.9299336677825636 1101 70-71 1.1745823105859188 1101 72-73 1.2086227990150462 1101 74-75 1.2623107200129695 1101 76-77 1.1503478775688833 1101 78-79 1.1736857200003747 1101 80-81 1.2022847749864631 1101 82-83 1.215776437482834 1101 84-85 1.3019603683827015 1101 86-87 1.5132532195313395 1101 88-89 1.363187825036011 1101 90-91 1.3276206746178616 1101 92-93 1.1609270222019425 1101 94-95 1.0181225922747323 1101 96-97 1.1794195011585522 1101 98-99 1.1108124043812886 1101 100-101 1.5071845210963062 1102 1 0.44865587450355804 1102 2 0.46657155628946256 1102 3 0.41249677673944163 1102 4 0.4597299314146426 1102 5 0.5014927265280278 1102 6 0.4556334541817222 1102 7 0.3173176787324081 1102 8 0.36837866128575314 1102 9 0.4582882261075909 1102 10-11 0.4324792949913885 1102 12-13 0.4982584491986657 1102 14-15 0.5101150453366401 1102 16-17 0.4636188684154092 1102 18-19 0.48088827721363003 1102 20-21 0.41264241666767276 1102 22-23 0.49177457358670296 1102 24-25 0.3951911504913568 1102 26-27 0.33199285637972764 1102 28-29 0.386091260470927 1102 30-31 0.363933253916187 1102 32-33 0.40267076915131383 1102 34-35 0.39702018142784823 1102 36-37 0.48738877271257763 1102 38-39 0.43047926160213734 1102 40-41 0.30223298902544826 1102 42-43 0.3713650378470206 1102 44-45 0.5957260031909613 1102 46-47 0.4904711778595896 1102 48-49 0.3577921903060073 1102 50-51 0.45545571131148677 1102 52-53 0.568718446324759 1102 54-55 0.4454886445526398 1102 56-57 0.43661109254062325 1102 58-59 0.5127428313120177 1102 60-61 0.5143950202752663 1102 62-63 0.2875425883176419 1102 64-65 0.24198462600147153 1102 66-67 0.24475183566357472 1102 68-69 0.2250715762792943 1102 70-71 0.3124826373833045 1102 72-73 0.3067022107797506 1102 74-75 0.3604829422351905 1102 76-77 0.33356846580418065 1102 78-79 0.1709576154252126 1102 80-81 0.40793640897339145 1102 82-83 0.5639895093782563 1102 84-85 0.5336695187095017 1102 86-87 0.497352239139186 1102 88-89 0.7541920734020238 1102 90-91 0.545732112526359 1102 92-93 0.49501787187514523 1102 94-95 0.37425821318976205 1102 96-97 0.333413292008224 1102 98-99 0.5719758030740998 1102 100-101 0.5851002073708162 1103 1 0.5182758033291819 1103 2 0.525859496186655 1103 3 0.4429837676449395 1103 4 0.5306230907661629 1103 5 0.45884915199263787 1103 6 0.459445870156415 1103 7 0.34114369692141366 1103 8 0.335890879553844 1103 9 0.4397900054670245 1103 10-11 0.5600544630420004 1103 12-13 0.4956577374548985 1103 14-15 0.6914713917186148 1103 16-17 0.6956705093802142 1103 18-19 0.5700705627019644 1103 20-21 0.5000870983600407 1103 22-23 0.6418005126930737 1103 24-25 0.7008069670196306 1103 26-27 0.7173236590685406 1103 28-29 0.7138884134958374 1103 30-31 0.8055036770083248 1103 32-33 0.8250937031173109 1103 34-35 0.7586964961767677 1103 36-37 0.8728832764690111 1103 38-39 0.8444877234447645 1103 40-41 0.9321914706387346 1103 42-43 0.7450259314808676 1103 44-45 0.8153045717951528 1103 46-47 0.8389159386345995 1103 48-49 0.8758508696258929 1103 50-51 0.9121334495479445 1103 52-53 0.8676677543516433 1103 54-55 1.0910552716779947 1103 56-57 1.146224299341732 1103 58-59 1.0455680586746112 1103 60-61 1.060374458788509 1103 62-63 1.2356653245770346 1103 64-65 0.8125097347401073 1103 66-67 1.1432075098430907 1103 68-69 1.175480908030977 1103 70-71 1.2466107512623097 1103 72-73 1.3876657536820858 1103 74-75 1.0973122028124962 1103 76-77 1.1785755832419014 1103 78-79 1.2334163738277404 1103 80-81 0.9922287774985001 1103 82-83 1.1291896675435389 1103 84-85 1.2943646551270547 1103 86-87 1.1775554024448418 1103 88-89 1.1119587005273708 1103 90-91 1.2740807088094783 1103 92-93 1.387482798723699 1103 94-95 1.0567967659774276 1103 96-97 1.2652830428979023 1103 98-99 1.255370820867693 1103 100-101 0.8883090646266467 1104 1 0.4858207319811072 1104 2 0.46559256370843727 1104 3 0.5241072414016834 1104 4 0.5105535159519334 1104 5 0.450992765575279 1104 6 0.4516094010774623 1104 7 0.45300842453482915 1104 8 0.39048939927872084 1104 9 0.5533924822575358 1104 10-11 0.42168601111790593 1104 12-13 0.4588852356102251 1104 14-15 0.5589599496708857 1104 16-17 0.6340786107035754 1104 18-19 0.6373810534729003 1104 20-21 0.6547152007363977 1104 22-23 0.7092522776085772 1104 24-25 0.8305685811824972 1104 26-27 0.6642593147944069 1104 28-29 0.7138112917087227 1104 30-31 0.6429275530181044 1104 32-33 0.690405404059554 1104 34-35 0.7372757847078191 1104 36-37 0.8872171210140252 1104 38-39 0.8272487266548509 1104 40-41 0.7616179948825419 1104 42-43 0.6259435618610851 1104 44-45 0.66127375797425 1104 46-47 0.8483826187030132 1104 48-49 0.791417180544201 1104 50-51 0.5864756254075445 1104 52-53 0.674046521295466 1104 54-55 0.7925569772351935 1104 56-57 0.9319903194051236 1104 58-59 0.7523504064779658 1104 60-61 0.7863348875146272 1104 62-63 0.7104639471618412 1104 64-65 0.7934574881646874 1104 66-67 0.9671374662766148 1104 68-69 0.874429951913811 1104 70-71 0.8605849411708846 1104 72-73 0.8162575407289907 1104 74-75 0.8563481511379649 1104 76-77 1.0494243814621242 1104 78-79 1.0194401613057273 1104 80-81 0.9827347689890118 1104 82-83 1.0282459716976646 1104 84-85 0.8732954460816202 1104 86-87 1.1137771512828785 1104 88-89 1.1141242092864445 1104 90-91 0.9747685826552157 1104 92-93 1.0625517258384427 1104 94-95 1.1305096774615322 1104 96-97 1.1850756895092012 1104 98-99 0.7369324606297383 1104 100-101 0.3414753733216145 1105 1 0.5297451845869539 1105 2 0.5762237170172853 1105 3 0.48794605649683476 1105 4 0.43727109896581595 1105 5 0.3535006112892134 1105 6 0.37902814713092425 1105 7 0.3535693845701644 1105 8 0.425863422468467 1105 9 0.5950569903229095 1105 10-11 0.4962879379026859 1105 12-13 0.5732021944602295 1105 14-15 0.65431686489692 1105 16-17 0.7013706500681707 1105 18-19 0.6127775872970247 1105 20-21 0.600871074741967 1105 22-23 0.7135119503873142 1105 24-25 0.7824363362381348 1105 26-27 0.7401942210499328 1105 28-29 0.7611776895838887 1105 30-31 0.8163196072141474 1105 32-33 0.8788145144128734 1105 34-35 0.7390015309350559 1105 36-37 0.830051092651928 1105 38-39 0.6737706945058548 1105 40-41 0.838104861656241 1105 42-43 0.9517080249584708 1105 44-45 0.9046768750635934 1105 46-47 0.9158576827875748 1105 48-49 0.9004187255599874 1105 50-51 0.7655906620317339 1105 52-53 0.9098259520108769 1105 54-55 0.9491035042948681 1105 56-57 1.1732902434124952 1105 58-59 1.1802295636850246 1105 60-61 1.2951774311926272 1105 62-63 1.0556823911986086 1105 64-65 1.1363652173585628 1105 66-67 1.0819303042003448 1105 68-69 1.0101263147175032 1105 70-71 1.102103562326441 1105 72-73 0.9708605125234975 1105 74-75 1.0833056867386048 1105 76-77 1.123184083696522 1105 78-79 1.0042329755465147 1105 80-81 1.1009079025291157 1105 82-83 1.27464394455642 1105 84-85 1.1191721722348973 1105 86-87 0.9869442027479813 1105 88-89 0.9466938171472847 1105 90-91 0.9068614529357646 1105 92-93 0.8251405405635452 1105 94-95 0.7493833079585279 1105 96-97 0.9693758393926082 1105 98-99 0.5720637484872881 1105 100-101 0.3857197676437494 1106 1 0.2266484418148984 1106 2 0.435497456934133 1106 3 0.14242654541596877 1106 4 0.460574224171566 1106 5 0.31610903293682924 1106 6 0.07051582050709015 1106 7 0.24184100068917758 1106 8 0.17914665521825412 1106 9 0.3929693030890107 1106 10-11 0.16669726996294543 1106 12-13 0.22026390994952294 1106 14-15 0.47471140483607854 1106 16-17 0.15559679788070468 1106 18-19 -0.03085764618417386 1106 20-21 0.24774397146479288 1106 22-23 0.2993463597072932 1106 24-25 0.4247653636123303 1106 26-27 0.1755745021893631 1106 28-29 0.2878914121584515 1106 30-31 0.3357629088270784 1106 32-33 0.3710601510246576 1106 34-35 0.3183202193497294 1106 36-37 0.2739139149196319 1106 38-39 0.43962306137461127 1106 40-41 0.4735431900114193 1106 42-43 0.031184984756386314 1106 44-45 0.22615171422622637 1106 46-47 0.010422031101910534 1106 48-49 0.3374627250045279 1106 50-51 0.10464569993492745 1106 52-53 0.3052707405985515 1106 54-55 0.17435250500012245 1106 56-57 -0.1801710841753419 1106 58-59 -0.5279113211339492 1106 60-61 0.14683567253161556 1106 62-63 0.2606890426217703 1106 64-65 0.44265736016908264 1106 66-67 -0.18726818708955406 1106 68-69 -0.44095208697440214 1106 70-71 -0.5156440216989893 1106 72-73 0.048920792501405685 1106 74-75 0.00798525546992579 1106 76-77 0.08192606914130707 1106 78-79 0.39874092979760434 1106 80-81 0.19885646964839765 1106 82-83 0.03471897467973761 1106 84-85 0.18554437650244537 1106 86-87 0.37365538665530096 1106 88-89 0.05911638132769781 1106 90-91 0.23245975757755488 1106 92-93 0.32711548279664626 1106 94-95 0.2204546485329537 1106 96-97 0.2796650174538051 1106 98-99 -0.08971627125279014 1106 100-101 -0.35218246232960126 1107 1 0.3055141211603214 1107 2 0.274640501311751 1107 3 0.2350810263679719 1107 4 0.17972339352459699 1107 5 0.2039783134522466 1107 6 0.1871775849395192 1107 7 0.17671143801918276 1107 8 0.08372903275677857 1107 9 0.17487960797980406 1107 10-11 0.18612892352036425 1107 12-13 0.24861298114517894 1107 14-15 0.2958318358269807 1107 16-17 0.33532496054021976 1107 18-19 0.18683775715418705 1107 20-21 0.3507199798177467 1107 22-23 0.2829219732895254 1107 24-25 0.19110318615258137 1107 26-27 0.34707339129801085 1107 28-29 0.31764846700881577 1107 30-31 0.35283994039464517 1107 32-33 0.282494246120109 1107 34-35 0.30741810119010893 1107 36-37 0.3030532600825637 1107 38-39 0.21431848894240346 1107 40-41 0.3987857379110409 1107 42-43 0.17687142714865445 1107 44-45 0.30572659754459863 1107 46-47 0.381209067904166 1107 48-49 0.27497614275771554 1107 50-51 0.48726254637835353 1107 52-53 0.5277505414213408 1107 54-55 0.5102078124575513 1107 56-57 0.36559563934597605 1107 58-59 0.34993451036104517 1107 60-61 0.4339477691608522 1107 62-63 0.43104006231466485 1107 64-65 0.46181523521395107 1107 66-67 0.38071439138422747 1107 68-69 0.40023116023174765 1107 70-71 0.39092988849771615 1107 72-73 0.40264812459847477 1107 74-75 0.4168787817597064 1107 76-77 0.4690454346006163 1107 78-79 0.42041289031377005 1107 80-81 0.424392278215592 1107 82-83 0.2389217530632486 1107 84-85 0.2678463389175221 1107 86-87 0.28880186766815896 1107 88-89 0.39961049836487916 1107 90-91 0.49246792233319425 1107 92-93 0.5341940977295287 1107 94-95 0.4454766381526163 1107 96-97 0.46285341087895304 1107 98-99 0.347565699062212 1107 100-101 0.3644722727497154 1108 1 0.3836534935511793 1108 2 0.286326913432319 1108 3 0.3144890386442043 1108 4 0.42331385998607374 1108 5 0.36841117890897834 1108 6 0.23146500180077112 1108 7 0.3358563692085994 1108 8 0.22184770890480365 1108 9 0.2618209642028333 1108 10-11 0.36720667594376977 1108 12-13 0.35255963967485826 1108 14-15 0.46048498581283326 1108 16-17 0.30638642793921633 1108 18-19 0.33443143197553127 1108 20-21 0.48789063095338747 1108 22-23 0.494104633110517 1108 24-25 0.5130604957294622 1108 26-27 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>>Per sequence quality scores pass #Quality Count 2 8538.0 3 2732.0 4 2734.0 5 2984.0 6 3483.0 7 4386.0 8 5549.0 9 7003.0 10 8904.0 11 11132.0 12 14163.0 13 17313.0 14 20244.0 15 24108.0 16 28184.0 17 33148.0 18 31071.0 19 27708.0 20 28239.0 21 30250.0 22 32992.0 23 36798.0 24 41910.0 25 46669.0 26 53570.0 27 61055.0 28 70372.0 29 81360.0 30 95635.0 31 112757.0 32 134111.0 33 163422.0 34 202997.0 35 255014.0 36 310784.0 37 333392.0 38 182824.0 39 11987.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48474794913419 19.330744150175253 15.613011138484481 22.571496762206078 2 15.107973189602836 20.51051326711235 40.629972630954406 23.75154091233041 3 18.80608257466613 24.742380136885114 30.212426817127618 26.239110471321137 4 12.626863554701043 17.334328805118666 37.78197591956378 32.25683172061651 5 13.49280961901107 37.59195326666073 34.43104618093014 14.484190933398056 6 31.01629226145163 36.87840144497875 17.821877369092142 14.283428924477478 7 27.842997298517375 33.28653599652723 20.88902334588353 17.981443359071868 8 26.124458569220423 34.79794725057944 20.182703837166613 18.894890343033524 9 26.497207969351678 16.88450743064241 20.470006598449856 36.14827800155605 10-11 24.5249073057123 27.055406208193023 27.566032369333755 20.85365411676092 12-13 25.781730099553446 24.384015038589173 27.820956645877782 22.013298215979603 14-15 22.5305279163023 25.815312015000043 26.57687520187816 25.077284866819504 16-17 21.613639761050507 28.910339975786776 27.34680074865221 22.129219514510503 18-19 21.655010654093168 27.24201789982198 29.930654833775268 21.172316612309587 20-21 22.372329188839295 26.354045560148876 30.137729998144376 21.135895252867453 22-23 22.039816067465043 26.37986827518337 30.00381748257313 21.576498174778454 24-25 21.77375835201267 26.521833101405495 30.14713806697293 21.557270479608903 26-27 21.431081679513223 26.828841635134687 29.909513333259792 21.830563352092298 28-29 21.459316571986857 27.186256588848007 29.82396252728118 21.530464311883957 30-31 21.654382541735327 26.777882074370584 29.791690264728395 21.776045119165698 32-33 21.366046838807 26.816555524191095 29.649423019759414 22.167974617242493 34-35 22.08023345052611 26.98227689117146 29.426010177018348 21.511479481284088 36-37 22.15699788857532 26.509262868728634 29.45168552516838 21.88205371752766 38-39 21.620237718473 26.89338669358706 29.40965049637758 22.07672509156236 40-41 22.08908065161252 26.71247781767036 29.439582433583972 21.758859097133147 42-43 21.824679754295808 26.738606090300543 29.225443365624415 22.211270789779235 44-45 21.828127187385615 26.995038431042385 29.302042967710918 21.874791413861086 46-47 22.137463052145335 27.27136142765073 28.628785881336416 21.96238963886752 48-49 22.02048083451347 27.212994220345205 28.58410665818123 22.182418286960097 50-51 22.116054087006106 27.415355158633268 28.458372254575643 22.01021849978498 52-53 22.27285740896004 27.30493912291473 28.459766620446526 21.962436847678706 54-55 21.8939296827665 27.439426491961505 28.519489715366685 22.14715410990531 56-57 22.048283655399615 27.480239191291005 28.393126233481464 22.078350919827912 58-59 22.07943444461794 27.098163484840043 28.36604359929168 22.456358471250336 60-61 21.775342763543808 27.36203064644469 28.692127854262505 22.170498735748996 62-63 21.85932532404462 27.369663977279913 28.731802239600164 22.039208459075308 64-65 21.687761619452843 27.25018572655263 28.60592556800427 22.456127085990264 66-67 21.569031280445017 27.374268236687676 28.528353391766764 22.528347091100546 68-69 22.051002951989997 27.11953747956133 28.594410121828655 22.23504944662002 70-71 21.88037254785346 27.16602420922988 28.258405348039684 22.695197894876976 72-73 21.877043111121612 27.230289639309657 28.2805373726871 22.61212987688163 74-75 21.93185810454211 27.571902437026292 28.085648815516056 22.410590642915544 76-77 22.015205823667817 27.12135142048291 28.31913838260461 22.544304373244668 78-79 21.958945066802134 27.372265719987283 28.190844106044633 22.477945107165958 80-81 22.133446904832084 27.51773619396468 27.854965573147194 22.49385132805604 82-83 22.201136614457802 27.412972479343907 27.940127987757656 22.445762918440636 84-85 22.105528035942335 27.41957650642809 27.983075536317426 22.49181992131215 86-87 22.205848618772563 27.475020354878822 27.742336277365865 22.57679474898275 88-89 22.14415712839848 27.401320602994094 27.65668103520433 22.797841233403098 90-91 22.126729504748308 27.416835658247813 27.712216397461898 22.74421843954198 92-93 22.258842842659906 27.093014187777133 27.831194519300528 22.81694845026243 94-95 22.01999325246932 26.9525248389288 28.01152870566172 23.01595320294016 96-97 22.270730489067322 26.725026274034636 27.891522507433148 23.112720729464893 98-99 22.160596412850122 26.820125960056 27.577891038505825 23.441386588588053 100-101 22.61852122944972 26.28614392100839 27.858075151086958 23.237259698454938 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1003.0 1 661.0 2 451.5 3 585.0 4 506.0 5 504.0 6 930.0 7 2170.5 8 3513.5 9 3924.5 10 3283.0 11 2522.5 12 2456.5 13 2587.5 14 2771.0 15 2966.5 16 3179.5 17 3476.5 18 3760.5 19 4230.5 20 4823.5 21 5608.0 22 6894.5 23 8393.0 24 10065.0 25 12347.5 26 15680.0 27 19766.0 28 23446.5 29 27654.0 30 32493.0 31 38610.0 32 46297.0 33 51085.0 34 53607.0 35 57467.5 36 62492.0 37 67143.0 38 70613.5 39 73602.5 40 77146.0 41 81735.5 42 85696.5 43 88252.0 44 90653.0 45 94372.5 46 96171.5 47 96867.0 48 97201.0 49 93613.0 50 88616.0 51 83993.0 52 80430.0 53 78895.0 54 78527.5 55 74285.5 56 67108.0 57 60908.5 58 55870.5 59 48812.5 60 39348.5 61 32747.0 62 27730.0 63 21524.0 64 15925.0 65 12137.5 66 9260.5 67 7120.0 68 5395.0 69 3989.5 70 3049.5 71 2303.0 72 1764.0 73 1421.5 74 1076.5 75 747.5 76 520.5 77 407.0 78 293.0 79 196.0 80 124.0 81 73.0 82 46.5 83 23.5 84 19.5 85 11.5 86 8.5 87 10.5 88 6.5 89 3.0 90 3.5 91 3.5 92 3.0 93 2.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18180586740339325 2 0.04933999390436468 3 0.04681983459879458 4 0.05363214022166376 5 0.06095635320347687 6 0.06418530731373857 7 0.036069780060972105 8 0.03457343547328985 9 0.04122823113956091 10-11 0.042133913390000166 12-13 0.038826204301439404 14-15 0.03394339564689733 16-17 0.032545494782088916 18-19 0.04177951598765437 20-21 0.05099384844864505 22-23 0.04725298697943944 24-25 0.06833963241901429 26-27 0.05347463026506563 28-29 0.04496909260876653 30-31 0.053336809053042264 32-33 0.049753457540434774 34-35 0.04937937139351421 36-37 0.04680014585421981 38-39 0.02671762638795805 40-41 0.024433732017285142 42-43 0.02102757920585055 44-45 0.004075570126976651 46-47 0.01187231297858416 48-49 0.010631922070373874 50-51 0.00811176276480377 52-53 0.008859935058644894 54-55 0.010730365793247706 56-57 0.008485848911724332 58-59 0.011163518173892568 60-61 0.010690988304098172 62-63 0.003760550213780389 64-65 0.006713861899995353 66-67 0.0042527688281495495 68-69 0.008584292634598164 70-71 0.02256330128268233 72-73 0.019767499553065497 74-75 0.020712559292654285 76-77 0.011714803021986027 78-79 0.004705609953369177 80-81 0.006005067095303762 82-83 0.005453782247210302 84-85 0.004764676187093477 86-87 0.008052696531079472 88-89 0.029828448030771147 90-91 0.036778574865663695 92-93 0.02268143375013093 94-95 0.032112342401444054 96-97 0.03520347529968238 98-99 0.02136228786362158 100-101 0.12409815705475283 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2539522.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.13900814186602 #Duplication Level Percentage of deduplicated Percentage of total 1 75.78338328475466 42.544039712409955 2 13.54644038913117 15.20967454597475 3 4.723671018287965 7.9554661726549405 4 2.1035847626320616 4.723726484660265 5 1.1197786642114547 3.143163177362735 6 0.6705707464753676 2.2587105957646703 7 0.43723279933854575 1.7182070977370245 8 0.29361219907706804 1.3186478107630968 9 0.19075290585945484 0.9637811041615683 >10 1.02737955952923 10.41862967593153 >50 0.06532240112252818 2.5095388798606786 >100 0.03319689936450684 3.349322514672666 >500 0.0029600492926140885 1.1807866095376005 >1k 0.0019028888309592612 1.670248851410579 >5k 1.4095472821920455E-4 0.593677224100811 >10k+ 7.047736410960227E-5 0.4423795429970788 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11181 0.44027970618092693 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8763 0.3450649374173565 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6242 0.24579428727138417 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.937748914953286E-5 2 0.0 0.0 0.0 0.0 3.937748914953286E-5 3 0.0 0.0 0.0 3.937748914953286E-5 3.937748914953286E-5 4 0.0 0.0 0.0 3.937748914953286E-5 3.937748914953286E-5 5 0.0 0.0 0.0 3.937748914953286E-5 3.937748914953286E-5 6 3.937748914953286E-5 0.0 0.0 3.937748914953286E-5 3.937748914953286E-5 7 3.937748914953286E-5 0.0 0.0 3.937748914953286E-5 3.937748914953286E-5 8 3.937748914953286E-5 0.0 0.0 3.937748914953286E-5 3.937748914953286E-5 9 3.937748914953286E-5 0.0 0.0 7.875497829906572E-5 7.875497829906572E-5 10-11 3.937748914953286E-5 0.0 0.0 7.875497829906572E-5 7.875497829906572E-5 12-13 7.875497829906572E-5 0.0 0.0 7.875497829906572E-5 7.875497829906572E-5 14-15 1.181324674485986E-4 0.0 0.0 1.181324674485986E-4 1.181324674485986E-4 16-17 1.5750995659813145E-4 0.0 0.0 1.181324674485986E-4 1.181324674485986E-4 18-19 1.9688744574766434E-4 0.0 0.0 1.3782121202336503E-4 1.181324674485986E-4 20-21 2.362649348971972E-4 0.0 0.0 1.9688744574766434E-4 1.181324674485986E-4 22-23 2.362649348971972E-4 0.0 0.0 3.150199131962629E-4 1.181324674485986E-4 24-25 2.7564242404673006E-4 0.0 0.0 7.28483549266358E-4 1.5750995659813145E-4 26-27 2.7564242404673006E-4 0.0 0.0 0.001456967098532716 1.5750995659813145E-4 28-29 3.150199131962629E-4 0.0 0.0 0.0038393051920794542 1.5750995659813145E-4 30-31 3.150199131962629E-4 0.0 0.0 0.011281650641341165 1.5750995659813145E-4 32-33 3.150199131962629E-4 0.0 0.0 0.02652073894221038 1.5750995659813145E-4 34-35 3.7408614692056223E-4 0.0 0.0 0.04888715277914505 1.5750995659813145E-4 36-37 4.725298697943944E-4 0.0 0.0 0.08623670123747698 1.5750995659813145E-4 38-39 5.119073589439272E-4 0.0 0.0 0.1433931267380239 1.5750995659813145E-4 40-41 5.119073589439272E-4 0.0 0.0 0.20596395699663164 1.5750995659813145E-4 42-43 5.315961035186937E-4 0.0 0.0 0.27253160240391694 1.5750995659813145E-4 44-45 5.512848480934601E-4 0.0 0.0 0.3559331244226276 1.5750995659813145E-4 46-47 5.90662337242993E-4 0.0 0.0 0.4549675096337027 1.5750995659813145E-4 48-49 5.90662337242993E-4 0.0 0.0 0.5600857169183807 1.5750995659813145E-4 50-51 5.90662337242993E-4 0.0 0.0 0.6737488393485073 1.5750995659813145E-4 52-53 5.90662337242993E-4 0.0 0.0 0.7859746834246759 1.5750995659813145E-4 54-55 5.90662337242993E-4 0.0 0.0 0.9145618742424755 1.5750995659813145E-4 56-57 5.90662337242993E-4 0.0 0.0 1.0665392936150977 1.5750995659813145E-4 58-59 5.90662337242993E-4 0.0 0.0 1.2312750194721684 1.5750995659813145E-4 60-61 5.90662337242993E-4 0.0 0.0 1.4071151972694074 1.5750995659813145E-4 62-63 6.300398263925258E-4 0.0 0.0 1.5825419114305763 1.5750995659813145E-4 64-65 6.497285709672922E-4 0.0 0.0 1.7711010182231144 1.5750995659813145E-4 66-67 7.481722938411245E-4 0.0 0.0 1.9899217254270685 1.5750995659813145E-4 68-69 7.481722938411245E-4 0.0 0.0 2.2328611447351117 1.5750995659813145E-4 70-71 8.269272721401901E-4 1.968874457476643E-5 0.0 2.4894448640334677 1.5750995659813145E-4 72-73 8.466160167149566E-4 3.937748914953286E-5 0.0 2.7568377041033703 1.771987011728979E-4 74-75 8.66304761289723E-4 7.875497829906572E-5 0.0 3.029073975338666 2.362649348971972E-4 76-77 8.66304761289723E-4 7.875497829906572E-5 0.0 3.3320246881105975 2.362649348971972E-4 78-79 8.859935058644895E-4 7.875497829906572E-5 0.0 3.6638784779182854 2.362649348971972E-4 80-81 0.001043503462462621 7.875497829906572E-5 0.0 4.015243813599567 2.362649348971972E-4 82-83 0.0011025696961869203 7.875497829906572E-5 0.0 4.388089569611919 2.362649348971972E-4 84-85 0.0011419471853364531 7.875497829906572E-5 0.0 4.77282732734743 2.7564242404673006E-4 86-87 0.001220702163635519 7.875497829906572E-5 0.0 5.168039497196717 2.7564242404673006E-4 88-89 0.0012600796527850516 7.875497829906572E-5 0.0 5.586504074388802 2.7564242404673006E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6865 0.0 41.878754 1 GTATCAA 11265 0.0 39.676353 1 ATCAACG 13915 0.0 31.733145 3 TATCAAC 14190 0.0 31.185152 2 TCAACGC 14205 0.0 31.0853 4 CAACGCA 14470 0.0 30.481361 5 AACGCAG 14865 0.0 29.664967 6 GTAAGGT 1475 0.0 28.03547 4 TAAGGTG 1605 0.0 27.539928 5 ACGCAGA 16610 0.0 26.402803 7 CGCAGAG 16650 0.0 26.2823 8 GGTAAGG 1645 0.0 24.560297 3 GCAGAGT 17585 0.0 23.882792 9 GAGTACT 9705 0.0 22.686604 12-13 AGGTAAG 1715 0.0 22.172081 2 GTACATG 10365 0.0 21.90516 1 TACATGG 10215 0.0 21.776499 2 AGAGTAC 15455 0.0 21.501474 10-11 AGTACTT 10110 0.0 21.14349 12-13 GTACTTT 10335 0.0 21.120865 14-15 >>END_MODULE