FastQCFastQC Report
Thu 26 May 2016
SRR938322_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938322_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2539522
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105250.4144480732988334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88790.3496327261587023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66000.2598914283869169No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29150.1147853808708883No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65700.043.214961
GTATCAA104000.038.2294541
ATCAACG133850.029.6927453
TCAACGC135400.029.3879474
TATCAAC135500.029.342162
CAACGCA139250.028.5071495
AACGCAG143100.027.812996
ACGCAGA162650.024.2947587
CGCAGAG163000.024.0969038
TAAGGTG15700.022.407565
GCAGAGT173250.022.2600489
GTAAGGT13750.022.1278784
GTACATG101400.021.7623181
TACATGG101450.021.6578372
GTACCGT1353.8006617E-421.1086676
AGTACTT103300.020.82769412-13
GAGTACT99800.020.77289412-13
AGAGTAC156000.020.6418410-11
ACATGGG104800.020.231873
GTACTTT104650.019.60594614-15
Produced by FastQC (version 0.11.5)