##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938322_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2539522 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.757391351600813 31.0 31.0 34.0 30.0 34.0 2 31.990861666093068 31.0 31.0 34.0 30.0 34.0 3 32.045399882340064 33.0 31.0 34.0 30.0 34.0 4 35.72416502003133 37.0 35.0 37.0 33.0 37.0 5 35.59567272896238 37.0 35.0 37.0 33.0 37.0 6 35.6263926833475 37.0 35.0 37.0 33.0 37.0 7 35.566599147398605 37.0 35.0 37.0 33.0 37.0 8 35.556676413907816 37.0 35.0 37.0 33.0 37.0 9 37.12696286939038 39.0 37.0 39.0 33.0 39.0 10-11 37.03632573374044 39.0 37.0 39.0 33.0 39.0 12-13 36.93163949751174 39.0 37.0 39.0 33.0 39.0 14-15 37.95780131064035 40.0 37.0 41.0 33.0 41.0 16-17 37.88301814278435 40.0 37.0 41.0 33.0 41.0 18-19 38.015108748811784 40.0 37.0 41.0 33.0 41.0 20-21 38.08111782453548 40.0 37.0 41.0 33.0 41.0 22-23 37.98098106651567 40.0 37.0 41.0 33.0 41.0 24-25 37.87866830844545 40.0 37.0 41.0 32.0 41.0 26-27 37.680667464192084 40.0 37.0 41.0 32.0 41.0 28-29 37.55586346564432 39.5 36.5 41.0 32.0 41.0 30-31 37.30394755390975 39.0 36.0 41.0 31.5 41.0 32-33 37.06371710896775 39.0 36.0 41.0 31.0 41.0 34-35 36.898072747548554 39.0 35.5 40.5 30.5 41.0 36-37 36.86678634798202 39.0 35.5 41.0 30.0 41.0 38-39 36.73350437602036 39.0 35.0 41.0 30.0 41.0 40-41 36.54972175866167 39.0 35.0 40.5 30.0 41.0 42-43 36.312876202686965 39.0 35.0 40.0 30.0 41.0 44-45 36.02128156401086 38.5 35.0 40.0 29.5 41.0 46-47 35.619897563399725 38.0 35.0 40.0 27.5 41.0 48-49 35.23439509482493 38.0 34.0 40.0 26.5 41.0 50-51 34.86135481401618 38.0 34.0 40.0 25.5 41.0 52-53 34.37321905460949 38.0 33.0 40.0 24.0 41.0 54-55 34.06649125307834 38.0 33.0 40.0 23.5 41.0 56-57 33.729818839923425 37.0 33.0 40.0 23.0 41.0 58-59 33.28859525532758 37.0 32.0 39.5 22.0 41.0 60-61 32.92512783901853 36.0 31.5 39.0 20.5 40.5 62-63 32.473959272650525 36.0 31.0 39.0 19.5 40.0 64-65 31.970438334458215 35.0 31.0 39.0 18.0 40.0 66-67 31.482282295644616 35.0 30.0 38.0 14.0 40.0 68-69 30.940943807535433 34.5 29.0 38.0 10.5 40.0 70-71 30.363518016382613 34.0 29.0 37.0 8.0 39.0 72-73 29.912009228508357 34.0 28.0 36.5 7.0 39.0 74-75 29.390314594636315 34.0 28.0 36.0 6.5 39.0 76-77 28.14487450787983 31.5 26.5 34.5 2.0 37.0 78-79 28.82812789178436 33.0 28.0 35.0 2.0 37.0 80-81 28.541021105546633 33.0 27.0 35.0 2.0 37.0 82-83 28.052575642187783 33.0 26.5 35.0 2.0 36.5 84-85 27.50302064719266 33.0 26.0 35.0 2.0 36.0 86-87 26.916950118959395 32.0 25.5 35.0 2.0 35.5 88-89 26.24649185949167 32.0 24.5 34.0 2.0 35.0 90-91 25.68458788701181 31.5 22.5 34.0 2.0 35.0 92-93 24.93274698939407 31.0 19.5 34.0 2.0 35.0 94-95 24.141062766930155 31.0 14.0 34.0 2.0 35.0 96-97 23.115192347221246 30.5 2.0 34.0 2.0 35.0 98-99 21.849546489457467 30.0 2.0 34.0 2.0 35.0 100-101 18.217236157040578 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2665961389396898 1101 2 0.2943226901458793 1101 3 0.29370095726378054 1101 4 0.23661442762765716 1101 5 0.22266724778222624 1101 6 0.14843426181248986 1101 7 0.25078964713841145 1101 8 0.2689837382379565 1101 9 0.26369684366304114 1101 10-11 0.2806142080214613 1101 12-13 0.2881103298168526 1101 14-15 0.3955096637876565 1101 16-17 0.4563513776934158 1101 18-19 0.4375225082299323 1101 20-21 0.45872105181174305 1101 22-23 0.5053438825331753 1101 24-25 0.5100735788433752 1101 26-27 0.558246574615282 1101 28-29 0.5241684801742394 1101 30-31 0.47371420899257544 1101 32-33 0.47741100469486497 1101 34-35 0.4963525884071629 1101 36-37 0.4932434808240984 1101 38-39 0.482286052242074 1101 40-41 0.6005744478779604 1101 42-43 0.6223636339787717 1101 44-45 0.6641826584616766 1101 46-47 0.7636552233919716 1101 48-49 0.7190321267913902 1101 50-51 0.6523936561443477 1101 52-53 0.7623682565052903 1101 54-55 0.7783612443764838 1101 56-57 0.6507445859537029 1101 58-59 0.6683307529358657 1101 60-61 0.6015597139064894 1101 62-63 0.6747644037204239 1101 64-65 0.6917193290410601 1101 66-67 0.5842586213717773 1101 68-69 0.7150349591910654 1101 70-71 0.673888913056512 1101 72-73 0.6088528923401917 1101 74-75 0.7866683149322888 1101 76-77 0.8648519404621844 1101 78-79 0.854052403548696 1101 80-81 0.9347577632402455 1101 82-83 1.0241076912307854 1101 84-85 0.8960732061005707 1101 86-87 0.9900119885101475 1101 88-89 1.0409985842185385 1101 90-91 1.1238301610698969 1101 92-93 1.0558072993052612 1101 94-95 1.2695905043471676 1101 96-97 1.3416160474714474 1101 98-99 1.2297027684728796 1101 100-101 0.7934631420313174 1102 1 0.24932032194622877 1102 2 0.22354099079947432 1102 3 0.25020618602195555 1102 4 0.15743011390216566 1102 5 0.10495025431817595 1102 6 0.2094564840347104 1102 7 0.24076611772664336 1102 8 0.1909948493490674 1102 9 0.16396547111402526 1102 10-11 0.2615057112894448 1102 12-13 0.2117773232809057 1102 14-15 0.381530252022948 1102 16-17 0.24249059337968504 1102 18-19 0.2867123775109661 1102 20-21 0.2294583067137026 1102 22-23 0.3693987844939599 1102 24-25 0.3128399187126547 1102 26-27 0.304382195530323 1102 28-29 0.21744331677554385 1102 30-31 0.19194460114943723 1102 32-33 0.11584629881252084 1102 34-35 0.26638298056403187 1102 36-37 0.29549380762148303 1102 38-39 0.33627624832050884 1102 40-41 0.34290287925050933 1102 42-43 0.22698258822713768 1102 44-45 0.31409082839631 1102 46-47 0.3575699292743195 1102 48-49 0.5195919307129557 1102 50-51 0.3189688195430378 1102 52-53 0.22204145911967288 1102 54-55 0.20753967574903243 1102 56-57 0.08440079510403109 1102 58-59 0.2279895764652693 1102 60-61 0.37882932174962747 1102 62-63 0.481676822021079 1102 64-65 0.5557212898253745 1102 66-67 0.4849125429404104 1102 68-69 0.29543659317799253 1102 70-71 0.1879291091349451 1102 72-73 0.04293034332058454 1102 74-75 0.07694315153359454 1102 76-77 0.3706398489589162 1102 78-79 0.2758337760977163 1102 80-81 0.333242730560503 1102 82-83 0.3339240311000644 1102 84-85 0.10935229106788924 1102 86-87 -0.021564808875471186 1102 88-89 0.2226476038263847 1102 90-91 -0.13022179971441972 1102 92-93 -0.1538639425248043 1102 94-95 -0.08616929957439723 1102 96-97 -0.08425257997953395 1102 98-99 -0.34278677401078284 1102 100-101 -0.06289241352423858 1103 1 0.3212213104792454 1103 2 0.29302015252347857 1103 3 0.34208819472104324 1103 4 0.24024292529006885 1103 5 0.24090422822090574 1103 6 0.09821678454874672 1103 7 0.2616576954253418 1103 8 0.2638680798749675 1103 9 0.3591237154793845 1103 10-11 0.3160647464812172 1103 12-13 0.40687166887204995 1103 14-15 0.5434981444705542 1103 16-17 0.47118082667348204 1103 18-19 0.46890043603212916 1103 20-21 0.5089065471249299 1103 22-23 0.561489650448884 1103 24-25 0.49908705196690306 1103 26-27 0.4698942556331218 1103 28-29 0.41584924797364664 1103 30-31 0.4489260167287199 1103 32-33 0.45764906670496686 1103 34-35 0.49563517510732424 1103 36-37 0.5625905256918671 1103 38-39 0.4280461178341497 1103 40-41 0.44499461511448857 1103 42-43 0.5460590611413281 1103 44-45 0.5380945452804582 1103 46-47 0.6302316137345869 1103 48-49 0.804052508016234 1103 50-51 0.5131471508993073 1103 52-53 0.6124368723264766 1103 54-55 0.5374833689083829 1103 56-57 0.5414157417232488 1103 58-59 0.4796343372402774 1103 60-61 0.4830847586812226 1103 62-63 0.29087342146750217 1103 64-65 0.24300820164822667 1103 66-67 0.5299685334504964 1103 68-69 0.3488772416556536 1103 70-71 0.13275939778658596 1103 72-73 0.13533176680812886 1103 74-75 0.3499089878325279 1103 76-77 0.22859065955598723 1103 78-79 0.2686714985176444 1103 80-81 0.1986292074288336 1103 82-83 0.2949642841645179 1103 84-85 0.13458732467801227 1103 86-87 0.24170921247684163 1103 88-89 0.39488904313045836 1103 90-91 0.4548935027766845 1103 92-93 0.2560390230742442 1103 94-95 0.22930124214169467 1103 96-97 0.09281827806712784 1103 98-99 0.03607095631740975 1103 100-101 -0.25537038900862186 1104 1 0.28315804158699365 1104 2 0.2546910103230964 1104 3 0.2774792824686543 1104 4 0.2102307386581188 1104 5 0.21138071117096757 1104 6 0.12622071675630764 1104 7 0.23072051054194986 1104 8 0.2684441269749982 1104 9 0.38272251914213484 1104 10-11 0.4204585422148597 1104 12-13 0.35990789727544126 1104 14-15 0.5344826924758976 1104 16-17 0.37792144070494516 1104 18-19 0.4345461924270211 1104 20-21 0.44286767805302674 1104 22-23 0.47855153732488276 1104 24-25 0.46257478790438 1104 26-27 0.5725274512897869 1104 28-29 0.5484614346982326 1104 30-31 0.46717068471055256 1104 32-33 0.47071267913270276 1104 34-35 0.4235751711145923 1104 36-37 0.5411042722837252 1104 38-39 0.49518839201437714 1104 40-41 0.6467724614449608 1104 42-43 0.6734017994727495 1104 44-45 0.5782566230077961 1104 46-47 0.45195985508454584 1104 48-49 0.5918555777258447 1104 50-51 0.6745303189573306 1104 52-53 0.7658876129658267 1104 54-55 0.6608658764518864 1104 56-57 0.5920451527768122 1104 58-59 0.7267035163325133 1104 60-61 0.6837328750491167 1104 62-63 0.6911511679796902 1104 64-65 0.6783911063033123 1104 66-67 0.573526131382426 1104 68-69 0.571907737262336 1104 70-71 0.4502259463079241 1104 72-73 0.28150465023194826 1104 74-75 0.1886945767581203 1104 76-77 0.23414566699874584 1104 78-79 0.22210452970958627 1104 80-81 0.26996338655425944 1104 82-83 0.25746842782009693 1104 84-85 0.3288581872022114 1104 86-87 0.33137544883635783 1104 88-89 0.361932023974763 1104 90-91 0.393296122972032 1104 92-93 0.4235751047223637 1104 94-95 0.5869759561850678 1104 96-97 0.4844509733199587 1104 98-99 0.4095056117760194 1104 100-101 0.1266229320438974 1105 1 0.17169606114258684 1105 2 0.2902891333316937 1105 3 0.16261376752309076 1105 4 0.15831684627517717 1105 5 0.10950188434092212 1105 6 0.23153593816662976 1105 7 0.16789485161595508 1105 8 0.24168506921260047 1105 9 0.22530527399499078 1105 10-11 0.28271708354114367 1105 12-13 0.3034980207790099 1105 14-15 0.44880455073408854 1105 16-17 0.3289830876935582 1105 18-19 0.37953997417511687 1105 20-21 0.3353116804817091 1105 22-23 0.37184442513080995 1105 24-25 0.326645074133431 1105 26-27 0.34731623647042653 1105 28-29 0.3556954775033745 1105 30-31 0.4610621144170679 1105 32-33 0.4469010372507327 1105 34-35 0.4648415097528016 1105 36-37 0.42969668859191046 1105 38-39 0.42323303376402777 1105 40-41 0.3027891567334464 1105 42-43 0.5304233767032542 1105 44-45 0.3655763477291387 1105 46-47 0.15819570637818714 1105 48-49 0.15007623700559947 1105 50-51 0.05425843288647769 1105 52-53 0.08228406715605985 1105 54-55 0.03457045664364955 1105 56-57 0.10062429775755533 1105 58-59 0.1890628137662631 1105 60-61 0.23006662273522238 1105 62-63 0.27306438835920943 1105 64-65 0.01727140354789114 1105 66-67 0.059069176753904884 1105 68-69 -0.06713702319804682 1105 70-71 0.016916068952991026 1105 72-73 0.15366969131148878 1105 74-75 0.04076923189751014 1105 76-77 0.16895948504685876 1105 78-79 0.22306849937292483 1105 80-81 0.24096555087892924 1105 82-83 0.11150462245715431 1105 84-85 0.2644799635470392 1105 86-87 0.37060350044977497 1105 88-89 0.2205510155018935 1105 90-91 0.23253225638868713 1105 92-93 0.2777058831006691 1105 94-95 0.2522120499328011 1105 96-97 0.23624340182486492 1105 98-99 -0.07497540175907602 1105 100-101 -0.42282023763341314 1106 1 0.13222999202586294 1106 2 0.1273325721419063 1106 3 0.17634528348119005 1106 4 0.11617899520668118 1106 5 0.1499645887891674 1106 6 0.011915490481428037 1106 7 0.13404044461325526 1106 8 0.10812385958797677 1106 9 -0.03394670180975368 1106 10-11 0.04070533207062965 1106 12-13 0.0584215401940682 1106 14-15 0.057795098439328285 1106 16-17 -0.11386405205072947 1106 18-19 0.18249576772712572 1106 20-21 0.17828037724840584 1106 22-23 0.2286340518432226 1106 24-25 0.17029422284613815 1106 26-27 0.15335451255724308 1106 28-29 0.23506781431062507 1106 30-31 0.2937337554915018 1106 32-33 0.15092312854327616 1106 34-35 -1.333637666505183E-4 1106 36-37 -0.29507016659163554 1106 38-39 0.0017893313694230528 1106 40-41 0.18384713408554632 1106 42-43 0.39081011951268607 1106 44-45 0.15658085494163032 1106 46-47 0.07164433200469489 1106 48-49 -0.0039338940879574125 1106 50-51 0.12969350668752355 1106 52-53 0.11754013185383627 1106 54-55 -0.023782736082594624 1106 56-57 0.26571759449727494 1106 58-59 0.24689317904395836 1106 60-61 -0.19497196759432 1106 62-63 -0.19497672367479169 1106 64-65 -0.5185616832500877 1106 66-67 -0.17962678205011073 1106 68-69 -0.7275879802008482 1106 70-71 -0.5305246640922086 1106 72-73 -0.3810023074188926 1106 74-75 -0.17085290459078806 1106 76-77 -0.021475130184050784 1106 78-79 -0.1451257233334573 1106 80-81 -1.0450404700462421 1106 82-83 -0.3838717974930326 1106 84-85 -0.3643343983519074 1106 86-87 -0.1613221088375525 1106 88-89 -0.014187511835356048 1106 90-91 -0.25800344552405363 1106 92-93 -0.4330433888653431 1106 94-95 -0.28138166210758087 1106 96-97 -0.3284594059181032 1106 98-99 -0.4290463113638374 1106 100-101 -0.6066127017305334 1107 1 0.09698926696851728 1107 2 0.15532940090348646 1107 3 0.07704897948406142 1107 4 0.07820752846382817 1107 5 -0.014498185503519778 1107 6 0.051934344361605156 1107 7 0.0400344683952909 1107 8 -0.009113917367123747 1107 9 0.0711309986028752 1107 10-11 0.016368415598506658 1107 12-13 0.061262018674668184 1107 14-15 0.11409577951180694 1107 16-17 0.11540470927864277 1107 18-19 0.12850019326134543 1107 20-21 0.17091477030954394 1107 22-23 0.17714558984314266 1107 24-25 0.2137359068255833 1107 26-27 0.06583836194933212 1107 28-29 0.10036040444271066 1107 30-31 0.09283167395776104 1107 32-33 0.10465075646482092 1107 34-35 0.07609412469478372 1107 36-37 0.11240495175224652 1107 38-39 0.10162840285244812 1107 40-41 0.051342700944417174 1107 42-43 0.1593790221052913 1107 44-45 0.07881564266079977 1107 46-47 0.006354178902846286 1107 48-49 -0.024613715646630396 1107 50-51 0.06528977050886198 1107 52-53 0.061707135196940044 1107 54-55 -0.013580383686331743 1107 56-57 0.0352620135289925 1107 58-59 -0.056081448794763844 1107 60-61 -0.014307379587670255 1107 62-63 0.03573833762286682 1107 64-65 0.013764677641123768 1107 66-67 0.09280882823015801 1107 68-69 -0.12791140087847097 1107 70-71 -0.2436836694683251 1107 72-73 -0.1482533119543028 1107 74-75 -0.1872883492093429 1107 76-77 -0.17204781820304405 1107 78-79 -0.1253264022083762 1107 80-81 -0.18207108816163498 1107 82-83 -0.1882310952000843 1107 84-85 -0.08826613389496529 1107 86-87 -0.00880106444753892 1107 88-89 -0.2421916235138859 1107 90-91 -0.2753418591497834 1107 92-93 -0.597934967784834 1107 94-95 -0.7141039206739528 1107 96-97 -0.7535224165322845 1107 98-99 -0.6648753327032075 1107 100-101 -1.000948282766437 1108 1 0.21135425051765466 1108 2 0.2545088479423292 1108 3 0.1635436860219528 1108 4 0.19547297104502093 1108 5 0.07638894479436686 1108 6 0.1154481507013756 1108 7 0.2087071891552128 1108 8 0.18031806363478609 1108 9 0.1469470187330728 1108 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38901.0 26 46205.0 27 55468.0 28 65974.0 29 78622.0 30 93204.0 31 111229.0 32 133758.0 33 164132.0 34 207407.0 35 271442.0 36 352647.0 37 403291.0 38 204454.0 39 8643.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.04853463626391 20.264453007789704 16.299311755908434 22.38770060003796 2 15.517362284788588 21.357331902464548 39.4468305389632 23.678475273783665 3 18.756785300396103 24.948247625415583 30.75510622772935 25.53986084645896 4 13.12132755691819 18.12285934124611 37.289970317248674 31.46584278458702 5 13.377267225713032 37.871482920604095 34.47282679166058 14.278423062022302 6 31.06371986539199 37.33383684016126 17.44686598501608 14.155577309430672 7 27.74085044350866 33.34044753303968 20.804584484796745 18.114117538654913 8 26.186542191798303 35.20067949795276 19.945643313977985 18.667134996270953 9 26.171972520812968 16.81040762789218 20.351900869533715 36.66571898176114 10-11 24.49982713282263 27.244516881523374 27.52208880253843 20.73356718311556 12-13 25.611768671427143 24.439225177021502 27.854887652085708 22.094118499465647 14-15 22.270647783323003 25.9292103002061 26.565648968585425 25.234492947885467 16-17 21.50869336827954 29.050289778942652 27.39629741345025 22.044719439327558 18-19 21.513556488189508 27.362275262825055 29.963119043662545 21.16104920532289 20-21 22.206206522329794 26.460648893768195 30.186704427053595 21.146440156848413 22-23 21.9252284485033 26.464409443981978 30.05024173840589 21.56012036910883 24-25 21.795995065203343 26.646228396974703 30.12141678909808 21.43635974872387 26-27 21.383992073381084 26.938473401247276 29.94121983608029 21.736314689291344 28-29 21.442460431530026 27.250502259874104 29.849554364955296 21.457482943640574 30-31 21.63395316126421 26.81622762078848 29.829117448086688 21.72070176986063 32-33 21.347974146315725 26.849737864054728 29.710729026958617 22.09155896267093 34-35 22.070599327119115 26.984941503943517 29.509623189067874 21.434835979869497 36-37 22.138630244006936 26.53281055696396 29.547024277835398 21.781534921193707 38-39 21.58293141792213 26.893294780957334 29.520743560739188 22.00303024038135 40-41 22.06110205485642 26.69265600642993 29.52776362840534 21.718478310308306 42-43 21.807159648908648 26.74423412390579 29.260703836566897 22.187902390618664 44-45 21.791325296650317 26.977301240154645 29.37202355403891 21.859349909156133 46-47 22.177953173865003 27.311832699224496 28.60471380047111 21.905500326439384 48-49 22.03593825924721 27.24508785511604 28.579905982306908 22.13906790332984 50-51 22.14580145397441 27.46961829824668 28.468605509225753 21.91597473855316 52-53 22.36023157113819 27.340184491412163 28.387094106686217 21.912489830763427 54-55 21.9441887095288 27.44979173245989 28.457225414861536 22.148794143149775 56-57 22.087621213755977 27.5098620921575 28.3126706521936 22.089846041892926 58-59 22.102820924567695 27.235814456421327 28.27817597169861 22.38318864731237 60-61 21.807726020881095 27.44707468570857 28.556752018686982 22.18844727472335 62-63 21.845429966741772 27.50366013761645 28.600874495279033 22.050035400362745 64-65 21.67122395474424 27.390922386181337 28.434268338687357 22.503585320387064 66-67 21.561362297080215 27.464920037038503 28.397478341867426 22.57623932401385 68-69 21.987639274737695 27.277116119543454 28.40145383235643 22.33379077336242 70-71 21.836871470542256 27.317279374558723 28.08618482476406 22.75966433013496 72-73 21.85012018996492 27.364958319903966 28.05020552100071 22.734715969130406 74-75 21.893194861080158 27.688458694195205 27.844039153825012 22.574307290899625 76-77 21.86789482430158 27.30637891697729 28.22326406307959 22.602462195641543 78-79 21.86096438621126 27.465838059288323 28.126395439771734 22.546802114728678 80-81 22.017902821356117 27.658331128164416 27.790089970891767 22.533676079587696 82-83 22.067771828896674 27.54200471627066 27.89278153058478 22.49744192424788 84-85 21.930573634552005 27.57183747076814 27.90863022321651 22.588958671463345 86-87 22.005157392620443 27.59069196363087 27.698687300437513 22.70546334331117 88-89 21.877815492691532 27.675525296118952 27.448277627268148 22.998381583921372 90-91 21.81896151825719 27.633121974232093 27.70870956487223 22.83920694263849 92-93 21.89084220582178 27.411559292427086 27.736820426714253 22.960778075036878 94-95 21.64381031773518 27.272116921284944 27.87449441735162 23.209578343628255 96-97 21.835393006123397 27.028123211400256 27.81868552796265 23.317798254513693 98-99 21.649161396972104 27.237341215134663 27.49542138064619 23.618076007247033 100-101 21.72625399583071 26.964995774795415 27.859475129571624 23.449275099802247 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1017.0 1 671.5 2 429.0 3 569.0 4 518.5 5 483.5 6 908.0 7 2185.5 8 3330.0 9 3638.5 10 3112.0 11 2388.5 12 2318.0 13 2473.5 14 2704.0 15 2953.0 16 3171.5 17 3434.0 18 3849.0 19 4290.5 20 4717.0 21 5601.0 22 6796.5 23 8238.0 24 9983.0 25 12460.5 26 15845.5 27 19641.5 28 23466.0 29 27803.5 30 32724.0 31 38806.5 32 46772.5 33 51480.0 34 53749.5 35 57951.5 36 63005.5 37 67326.0 38 70588.5 39 73513.5 40 76977.0 41 82161.5 42 86636.0 43 89105.5 44 91660.5 45 95645.0 46 97184.5 47 98071.0 48 98756.0 49 94394.0 50 89243.5 51 84733.5 52 80526.5 53 79455.5 54 79384.0 55 74656.0 56 66946.0 57 60170.5 58 55091.0 59 48095.0 60 38387.0 61 31315.5 62 26590.5 63 20533.5 64 14630.5 65 11005.5 66 8322.0 67 6491.5 68 4924.5 69 3519.5 70 2691.5 71 1991.0 72 1493.5 73 1278.5 74 977.0 75 674.0 76 475.0 77 309.0 78 234.5 79 171.5 80 87.5 81 48.0 82 30.5 83 16.0 84 9.0 85 6.0 86 2.5 87 3.5 88 3.0 89 0.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.300398263925258E-4 2 0.04741049693603757 3 9.844372287383216E-4 4 0.0 5 0.08761491335771061 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.968874457476643E-4 26-27 0.009056822504392558 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011458849342514064 36-37 1.5750995659813145E-4 38-39 0.004705609953369177 40-41 0.005808179649556097 42-43 4.725298697943944E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 9.844372287383216E-5 68-69 0.0010631922070373874 70-71 1.181324674485986E-4 72-73 1.968874457476643E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0013388346310841174 82-83 6.103510818177595E-4 84-85 0.004981252377415907 86-87 0.0023823380935467384 88-89 7.875497829906572E-5 90-91 0.003307709088560761 92-93 9.450597395887888E-4 94-95 5.90662337242993E-5 96-97 1.968874457476643E-5 98-99 3.937748914953286E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2539522.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.14284354539345 #Duplication Level Percentage of deduplicated Percentage of total 1 74.03004949897397 40.081973876806806 2 14.518205267971092 15.72113832767332 3 4.961602564774235 8.059058141969828 4 2.2837441653587436 4.945936121708944 5 1.2469720638596538 3.3757306679514807 6 0.7245581124555915 2.353778191333721 7 0.4732107098725003 1.7934681400041947 8 0.30446004100698004 1.3187465892851997 9 0.22400185064962225 1.091528743824098 >10 1.1173971569416028 10.92232420578063 >50 0.07381109171255602 2.7130493942845417 >100 0.036592910238659085 3.6444663183737425 >500 0.0029888812645845727 1.1327857147883755 >1k 0.002186986291153823 1.819640662824874 >5k 1.4579908607692153E-4 0.610954358159434 >10k+ 7.289954303846076E-5 0.4154205452308316 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10525 0.4144480732988334 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8879 0.3496327261587023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6600 0.2598914283869169 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2915 0.1147853808708883 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 3.937748914953286E-5 0.0 0.0 0.0 0.0 6 3.937748914953286E-5 0.0 0.0 0.0 0.0 7 7.875497829906572E-5 0.0 0.0 0.0 3.937748914953286E-5 8 7.875497829906572E-5 0.0 0.0 0.0 3.937748914953286E-5 9 7.875497829906572E-5 0.0 0.0 3.937748914953286E-5 7.875497829906572E-5 10-11 7.875497829906572E-5 0.0 0.0 5.9066233724299293E-5 7.875497829906572E-5 12-13 9.844372287383217E-5 0.0 0.0 7.875497829906572E-5 7.875497829906572E-5 14-15 1.181324674485986E-4 0.0 0.0 2.362649348971972E-4 7.875497829906572E-5 16-17 1.181324674485986E-4 0.0 0.0 2.362649348971972E-4 7.875497829906572E-5 18-19 1.5750995659813145E-4 0.0 0.0 3.150199131962629E-4 7.875497829906572E-5 20-21 1.968874457476643E-4 0.0 0.0 3.543974023457958E-4 7.875497829906572E-5 22-23 1.968874457476643E-4 0.0 0.0 4.725298697943944E-4 7.875497829906572E-5 24-25 2.7564242404673006E-4 0.0 0.0 9.647484841635552E-4 7.875497829906572E-5 26-27 2.7564242404673006E-4 0.0 0.0 0.0016932320334299132 7.875497829906572E-5 28-29 2.9533116862149645E-4 0.0 0.0 0.0040361926378271185 7.875497829906572E-5 30-31 3.150199131962629E-4 0.0 0.0 0.011399783108789766 7.875497829906572E-5 32-33 3.150199131962629E-4 0.0 0.0 0.026737315132532816 7.875497829906572E-5 34-35 3.3470865777102934E-4 0.0 0.0 0.049182483947766545 7.875497829906572E-5 36-37 3.543974023457958E-4 0.0 0.0 0.08688642980844427 7.875497829906572E-5 38-39 4.331523806448615E-4 0.0 0.0 0.14455476266793515 9.844372287383217E-5 40-41 4.331523806448615E-4 0.0 0.0 0.2075587453071877 1.181324674485986E-4 42-43 4.7252986979439435E-4 0.0 0.0 0.27459892058426744 1.181324674485986E-4 44-45 6.103510818177595E-4 0.0 0.0 0.3587092374076696 1.181324674485986E-4 46-47 7.481722938411245E-4 0.0 0.0 0.4577042451295953 1.181324674485986E-4 48-49 7.875497829906572E-4 0.0 0.0 0.5621530350987312 1.181324674485986E-4 50-51 7.875497829906572E-4 0.0 0.0 0.6759342899963063 1.181324674485986E-4 52-53 7.875497829906572E-4 0.0 0.0 0.7880026241158768 1.181324674485986E-4 54-55 7.875497829906572E-4 0.0 0.0 0.9168457686131485 1.181324674485986E-4 56-57 8.66304761289723E-4 0.0 0.0 1.06886256547492 1.181324674485986E-4 58-59 8.66304761289723E-4 0.0 0.0 1.233165138951346 1.181324674485986E-4 60-61 9.056822504392559E-4 0.0 0.0 1.4082965219438934 1.181324674485986E-4 62-63 9.056822504392559E-4 0.0 0.0 1.583073507534095 1.181324674485986E-4 64-65 9.056822504392559E-4 0.0 0.0 1.7716916805603575 1.181324674485986E-4 66-67 9.450597395887888E-4 0.0 0.0 1.9899807916607928 1.181324674485986E-4 68-69 0.0010238147178878545 0.0 0.0 2.2317585750389246 1.181324674485986E-4 70-71 0.001043503462462621 0.0 0.0 2.4883816718264304 1.181324674485986E-4 72-73 0.0010631922070373874 0.0 0.0 2.757448055185188 1.181324674485986E-4 74-75 0.0010631922070373874 0.0 0.0 3.0310625385407173 1.181324674485986E-4 76-77 0.0010631922070373874 0.0 0.0 3.336336523172471 1.181324674485986E-4 78-79 0.0010631922070373874 0.0 0.0 3.6711633134109487 1.181324674485986E-4 80-81 0.0011616359299112195 0.0 0.0 4.026052934371114 1.181324674485986E-4 82-83 0.0012207021636355187 0.0 0.0 4.401359783455312 1.181324674485986E-4 84-85 0.001279768397359818 0.0 0.0 4.78972027019258 1.181324674485986E-4 86-87 0.0013388346310841174 0.0 0.0 5.190858752158871 1.181324674485986E-4 88-89 0.0013388346310841174 0.0 0.0 5.6165687873544705 1.181324674485986E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6570 0.0 43.21496 1 GTATCAA 10400 0.0 38.229454 1 ATCAACG 13385 0.0 29.692745 3 TCAACGC 13540 0.0 29.387947 4 TATCAAC 13550 0.0 29.34216 2 CAACGCA 13925 0.0 28.507149 5 AACGCAG 14310 0.0 27.81299 6 ACGCAGA 16265 0.0 24.294758 7 CGCAGAG 16300 0.0 24.096903 8 TAAGGTG 1570 0.0 22.40756 5 GCAGAGT 17325 0.0 22.260048 9 GTAAGGT 1375 0.0 22.127878 4 GTACATG 10140 0.0 21.762318 1 TACATGG 10145 0.0 21.657837 2 GTACCGT 135 3.8006617E-4 21.108667 6 AGTACTT 10330 0.0 20.827694 12-13 GAGTACT 9980 0.0 20.772894 12-13 AGAGTAC 15600 0.0 20.64184 10-11 ACATGGG 10480 0.0 20.23187 3 GTACTTT 10465 0.0 19.605946 14-15 >>END_MODULE