FastQCFastQC Report
Thu 26 May 2016
SRR938321_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938321_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2540539
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115870.45608431911495945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88850.3497289354739289No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64250.252899089523916No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65750.045.3646851
GTATCAA108300.039.46421
TATCAAC135500.031.4608382
ATCAACG137850.031.0279393
TCAACGC138750.030.7581714
CAACGCA142150.030.0224865
AACGCAG146750.029.1427296
ACGCAGA163750.025.9089877
CGCAGAG166000.025.414718
GCAGAGT174950.023.461899
GTACATG103300.022.9242631
GAGTACT101000.022.71406612-13
TACATGG103350.022.2102682
AGTACTT105700.021.20977812-13
GTACTTT108750.020.85658514-15
CAGAGTA169450.020.44849610-11
AGAGTAC157650.020.33702910-11
ACATGGG109600.020.249933
GTAAGGT15250.019.3214174
TAAGGTG16350.018.8935155
Produced by FastQC (version 0.11.5)