##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938321_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2540539 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.239135081177654 31.0 31.0 34.0 28.0 34.0 2 31.405319894715255 31.0 31.0 34.0 28.0 34.0 3 31.45501249931609 33.0 31.0 34.0 28.0 34.0 4 35.12337618119619 37.0 35.0 37.0 32.0 37.0 5 35.13647064658326 37.0 35.0 37.0 32.0 37.0 6 35.14174000084234 37.0 35.0 37.0 32.0 37.0 7 35.068492552171016 37.0 35.0 37.0 32.0 37.0 8 35.028006655280635 37.0 35.0 37.0 32.0 37.0 9 36.53545330341317 39.0 37.0 39.0 32.0 39.0 10-11 36.40369248415395 39.0 37.0 39.0 32.0 39.0 12-13 36.245239888071 39.0 35.0 39.0 32.0 39.0 14-15 37.24977337486258 39.5 36.5 41.0 31.5 41.0 16-17 37.092186933560164 39.0 36.0 41.0 31.0 41.0 18-19 36.984410001184784 39.0 36.0 41.0 31.0 41.0 20-21 36.98276704274172 39.0 36.0 41.0 31.0 41.0 22-23 37.077295408572745 39.0 36.0 41.0 31.0 41.0 24-25 36.99681898211364 39.0 36.0 41.0 31.0 41.0 26-27 36.80498055727544 39.0 36.0 41.0 30.0 41.0 28-29 36.6270905504698 39.0 36.0 41.0 30.0 41.0 30-31 36.35537498145079 39.0 35.5 40.0 30.0 41.0 32-33 36.03522028199528 38.5 35.0 40.0 29.0 41.0 34-35 35.781929346489065 38.0 35.0 40.0 28.0 41.0 36-37 35.486849444153385 38.0 34.5 40.0 27.0 41.0 38-39 35.16948667192277 38.0 34.0 40.0 26.0 41.0 40-41 35.040789375797814 38.0 34.0 40.0 25.0 41.0 42-43 34.79831661706433 38.0 34.0 40.0 24.5 41.0 44-45 34.47173847754354 38.0 33.0 40.0 24.0 41.0 46-47 34.05003642927741 38.0 33.0 40.0 23.0 41.0 48-49 33.63772234946994 38.0 33.0 40.0 20.5 41.0 50-51 32.85262989467983 37.0 31.5 39.5 18.5 40.5 52-53 32.87372108832024 37.0 32.0 39.5 18.0 40.0 54-55 32.95610478721248 37.0 32.0 40.0 16.0 41.0 56-57 32.63478812960557 37.0 31.0 40.0 13.0 41.0 58-59 32.21343148048504 36.5 31.0 39.5 8.5 41.0 60-61 31.73958360804538 36.0 30.5 39.0 7.5 41.0 62-63 31.16348597679469 35.5 29.5 39.0 3.5 40.0 64-65 30.67557907987242 35.0 29.0 39.0 2.0 40.0 66-67 30.130364658838143 35.0 28.0 38.0 2.0 40.0 68-69 29.518006021556843 34.0 27.5 38.0 2.0 40.0 70-71 28.879157139488903 34.0 26.0 37.0 2.0 39.5 72-73 28.245268818939604 34.0 26.0 36.5 2.0 39.0 74-75 27.549742397184218 33.0 25.0 36.0 2.0 39.0 76-77 26.804142349320358 32.5 23.0 35.5 2.0 38.0 78-79 26.01048616061395 32.0 20.5 35.0 2.0 37.0 80-81 25.250084726115205 31.5 18.0 35.0 2.0 36.5 82-83 24.508855010688677 31.0 10.5 35.0 2.0 36.0 84-85 23.759515992472465 31.0 6.0 34.0 2.0 36.0 86-87 23.03995195507725 30.0 2.0 34.0 2.0 35.0 88-89 22.268050205094273 29.5 2.0 34.0 2.0 35.0 90-91 21.45407057321301 29.0 2.0 34.0 2.0 35.0 92-93 20.596862122565327 28.5 2.0 34.0 2.0 35.0 94-95 19.706868109483853 26.5 2.0 34.0 2.0 35.0 96-97 18.64757990331973 25.5 2.0 33.5 2.0 35.0 98-99 17.409363328018188 21.5 2.0 33.0 2.0 35.0 100-101 14.575682561850064 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5866085945884478 1101 2 0.6011040319011904 1101 3 0.6617040011940638 1101 4 0.4378791373670978 1101 5 0.47067866949368664 1101 6 0.3785559619101093 1101 7 0.47727480965488667 1101 8 0.4073912964073969 1101 9 0.6526807541842174 1101 10-11 0.5842606364908534 1101 12-13 0.6472046086012355 1101 14-15 0.7886579068697515 1101 16-17 0.7712462413251444 1101 18-19 0.9184518430494251 1101 20-21 1.0284420873864804 1101 22-23 0.9038825950390006 1101 24-25 0.9950684122037288 1101 26-27 1.0312960013480748 1101 28-29 1.0986416769832417 1101 30-31 0.9418275980927078 1101 32-33 1.128368304481235 1101 34-35 1.0955704012979481 1101 36-37 1.3193541572466998 1101 38-39 1.3550296749693729 1101 40-41 1.4596612267460003 1101 42-43 1.4909660863367762 1101 44-45 1.6194812214157324 1101 46-47 1.6502197083997103 1101 48-49 1.807376925334303 1101 50-51 1.7994023784232098 1101 52-53 1.9168487771952982 1101 54-55 2.113860292714584 1101 56-57 2.090007171780435 1101 58-59 2.274424369739343 1101 60-61 2.3090936323959035 1101 62-63 2.240309098110785 1101 64-65 2.3576096517158156 1101 66-67 2.4087499827926777 1101 68-69 2.6128095421641895 1101 70-71 2.7188359907912734 1101 72-73 2.930255191166971 1101 74-75 3.068646019459859 1101 76-77 3.303769390320536 1101 78-79 3.4131761479953227 1101 80-81 3.785375727166006 1101 82-83 3.9623574001409416 1101 84-85 4.09842732319818 1101 86-87 4.207449228237216 1101 88-89 4.517123372630781 1101 90-91 4.778409210210043 1101 92-93 4.896586281457246 1101 94-95 5.261909743242789 1101 96-97 5.48216246184894 1101 98-99 5.67360523761975 1101 100-101 5.522298446995471 1102 1 0.6581757034012519 1102 2 0.5623530067245497 1102 3 0.6373879195137242 1102 4 0.5504203889105668 1102 5 0.46776905845450756 1102 6 0.5556934470621613 1102 7 0.6031407497989107 1102 8 0.6653032479134424 1102 9 0.6835996905821986 1102 10-11 0.7553738642358852 1102 12-13 0.8615714894955957 1102 14-15 1.1058100737248324 1102 16-17 1.135354758147173 1102 18-19 1.0710581061126874 1102 20-21 0.9927085694235629 1102 22-23 0.7497351443224503 1102 24-25 0.8259823938529038 1102 26-27 0.8990531039152785 1102 28-29 0.9063682491706686 1102 30-31 0.9682104945606298 1102 32-33 1.0208835044770623 1102 34-35 0.9822712687701483 1102 36-37 1.155460194086551 1102 38-39 1.101359575183345 1102 40-41 1.4483536424598498 1102 42-43 1.4705455129158693 1102 44-45 1.6307020975978688 1102 46-47 1.647234635232408 1102 48-49 1.4436008982344362 1102 50-51 1.595059899666694 1102 52-53 1.678701546834688 1102 54-55 1.932389521168922 1102 56-57 1.9491594729352855 1102 58-59 2.009277833043562 1102 60-61 2.0209050689992445 1102 62-63 2.458080249998602 1102 64-65 2.3525173434417788 1102 66-67 2.5275431820087846 1102 68-69 2.686125155751153 1102 70-71 2.647499505976924 1102 72-73 2.7472849561684534 1102 74-75 2.9295155322385504 1102 76-77 3.1553718929902317 1102 78-79 3.4780021906765697 1102 80-81 3.8890509120889654 1102 82-83 4.060230965382598 1102 84-85 4.057176105699046 1102 86-87 4.399336544210673 1102 88-89 4.7013143664621175 1102 90-91 4.884747898583722 1102 92-93 4.848151656107973 1102 94-95 5.143084109980254 1102 96-97 5.422138939830948 1102 98-99 5.762133396891453 1102 100-101 5.565429046350573 1103 1 0.6133288774195691 1103 2 0.5635152912324486 1103 3 0.5544469935366898 1103 4 0.4981114213473603 1103 5 0.5213697693875687 1103 6 0.4837057840649166 1103 7 0.5135021059966718 1103 8 0.44952757119450837 1103 9 0.6896017448524887 1103 10-11 0.7016579206894349 1103 12-13 0.8063285952696546 1103 14-15 0.813048104470603 1103 16-17 0.9682869112709227 1103 18-19 1.136663874002906 1103 20-21 0.9893371856173943 1103 22-23 0.9236126221792915 1103 24-25 0.9886785145446026 1103 26-27 1.0004032697459238 1103 28-29 1.1025735870488163 1103 30-31 1.0755947237208332 1103 32-33 1.140756720149838 1103 34-35 1.1893513881568296 1103 36-37 1.364310770107359 1103 38-39 1.317176745127263 1103 40-41 1.2850519577874948 1103 42-43 1.489131395355848 1103 44-45 1.3562635364975435 1103 46-47 1.4098025774245286 1103 48-49 1.2499643466391461 1103 50-51 1.5654088410915392 1103 52-53 1.717784845313716 1103 54-55 1.910136823160876 1103 56-57 1.9260655294286622 1103 58-59 2.160528936763349 1103 60-61 2.3439336191832787 1103 62-63 2.3066119588840337 1103 64-65 2.4660814549793564 1103 66-67 2.6545304703814807 1103 68-69 2.887791713349916 1103 70-71 2.9677726689177355 1103 72-73 3.0603337929829486 1103 74-75 3.2546170839429003 1103 76-77 3.352999144412621 1103 78-79 3.456399395123885 1103 80-81 3.839399881966308 1103 82-83 4.2293694427377275 1103 84-85 4.312591090111823 1103 86-87 4.24627633588149 1103 88-89 4.685698924722875 1103 90-91 4.895538033913287 1103 92-93 5.004181830929596 1103 94-95 5.218207475265746 1103 96-97 5.479744102631397 1103 98-99 5.560445025711559 1103 100-101 5.499888956307906 1104 1 0.49078746955207464 1104 2 0.455819862884848 1104 3 0.40558501043557627 1104 4 0.3415519115269845 1104 5 0.3835277348064068 1104 6 0.42255223921572593 1104 7 0.4092584990356727 1104 8 0.4564172248232907 1104 9 0.6417553736365562 1104 10-11 0.6541088187925013 1104 12-13 0.6299517137297315 1104 14-15 0.6908913980747613 1104 16-17 0.8265821241856983 1104 18-19 0.930841592093671 1104 20-21 0.8281769959245935 1104 22-23 0.8041663125155907 1104 24-25 0.8934544084509355 1104 26-27 0.974853846098668 1104 28-29 0.9413521948491592 1104 30-31 0.9625344584866511 1104 32-33 0.974693335336454 1104 34-35 1.1465166279307653 1104 36-37 1.3603238639465403 1104 38-39 1.3000120649190947 1104 40-41 1.4507288924861683 1104 42-43 1.4002793445472719 1104 44-45 1.462946500517468 1104 46-47 1.5765199547397302 1104 48-49 1.711938354808808 1104 50-51 1.6295525655613545 1104 52-53 1.5544878138101552 1104 54-55 1.7616752793189008 1104 56-57 1.912588737419263 1104 58-59 1.9403188198141024 1104 60-61 2.2632617205765975 1104 62-63 2.435319082156383 1104 64-65 2.434497464156234 1104 66-67 2.462118532045629 1104 68-69 2.7074541552949647 1104 70-71 2.9150353430825824 1104 72-73 3.047680611851508 1104 74-75 3.078441296354068 1104 76-77 3.13763976329237 1104 78-79 3.3791880611190734 1104 80-81 3.8398615237322993 1104 82-83 3.953054113433268 1104 84-85 4.2556377858759085 1104 86-87 4.35742533425875 1104 88-89 4.912994543322842 1104 90-91 5.236042552406936 1104 92-93 5.208484542025786 1104 94-95 5.528215658915233 1104 96-97 5.799742284042619 1104 98-99 6.1682243889257045 1104 100-101 6.0045893088343405 1105 1 0.4173908449038457 1105 2 0.4430457300355286 1105 3 0.35560311943928724 1105 4 0.3245939743714672 1105 5 0.2785392246570382 1105 6 0.3137920607067386 1105 7 0.28250900275738644 1105 8 0.24869446326267308 1105 9 0.3103429129768003 1105 10-11 0.37083820783218613 1105 12-13 0.4350546020117463 1105 14-15 0.5048421998665162 1105 16-17 0.6148353130355488 1105 18-19 0.6644061101027603 1105 20-21 0.7661989307155253 1105 22-23 0.5735595984463515 1105 24-25 0.4770014419047115 1105 26-27 0.6856015398025619 1105 28-29 0.6931974398827165 1105 30-31 0.7939666933458582 1105 32-33 0.7858002950220566 1105 34-35 0.8433760687580332 1105 36-37 0.9650938171545391 1105 38-39 1.005592968454316 1105 40-41 1.2285761690585275 1105 42-43 1.2888798307844311 1105 44-45 1.2088568838455274 1105 46-47 1.3494543834943329 1105 48-49 1.4070524623471528 1105 50-51 1.3333569133874406 1105 52-53 1.465509640363372 1105 54-55 1.6175146004185947 1105 56-57 1.6682759012578714 1105 58-59 1.5158125503521234 1105 60-61 1.8867037218088463 1105 62-63 2.1348865475619334 1105 64-65 2.273153526301339 1105 66-67 2.3010113642105523 1105 68-69 2.3734897821579004 1105 70-71 2.550759724046067 1105 72-73 2.888885464231901 1105 74-75 2.953398625713138 1105 76-77 2.987623319484399 1105 78-79 3.342466852353482 1105 80-81 3.51660123698559 1105 82-83 3.7440777717017966 1105 84-85 3.981945172684231 1105 86-87 4.292103347846744 1105 88-89 4.5007337070615385 1105 90-91 4.719711665523679 1105 92-93 4.8960433442504865 1105 94-95 5.046429257736275 1105 96-97 5.222651006337927 1105 98-99 5.346876995789639 1105 100-101 5.277464935364243 1106 1 0.3687787452184459 1106 2 0.224087244550347 1106 3 0.2150023420260787 1106 4 0.2689954772427683 1106 5 0.33614750337999766 1106 6 0.12101437264560388 1106 7 0.26166215756652633 1106 8 0.29678672186346233 1106 9 0.2988659641453424 1106 10-11 0.17644156125656707 1106 12-13 0.11283438190412909 1106 14-15 0.4807301119232008 1106 16-17 0.2779287125034102 1106 18-19 0.5562794544321008 1106 20-21 0.5742575402609518 1106 22-23 0.517575714389757 1106 24-25 0.5217157482038957 1106 26-27 0.6651242804581159 1106 28-29 0.5296995907143298 1106 30-31 0.6760349592366026 1106 32-33 0.786646595148099 1106 34-35 0.8521238518952927 1106 36-37 0.8548473521753905 1106 38-39 0.8838547683105631 1106 40-41 1.045514334062993 1106 42-43 0.5651594936908282 1106 44-45 0.857688067700245 1106 46-47 1.2293446370103567 1106 48-49 1.1526567899299494 1106 50-51 0.6158539394499485 1106 52-53 1.1328997018020317 1106 54-55 1.0210065959386228 1106 56-57 1.0927665918115181 1106 58-59 0.5161924286461002 1106 60-61 1.6516770585710567 1106 62-63 1.9183423658535617 1106 64-65 2.1239399272817643 1106 66-67 2.2401930015563067 1106 68-69 1.5757601511290993 1106 70-71 2.052461964407481 1106 72-73 2.5459932255781688 1106 74-75 2.797251686092693 1106 76-77 2.9689825372119962 1106 78-79 3.1961176704870944 1106 80-81 3.7716701949347105 1106 82-83 3.8908387239532516 1106 84-85 4.120454988634972 1106 86-87 4.314274294673641 1106 88-89 4.377947924207831 1106 90-91 4.765011554104312 1106 92-93 4.915419311704319 1106 94-95 4.774648844061058 1106 96-97 5.257227614184945 1106 98-99 5.361887080938626 1106 100-101 4.495743901863342 1107 1 0.39672946754366833 1107 2 0.24764072426767214 1107 3 0.41506682320754607 1107 4 0.31922932068382437 1107 5 0.3813891548235091 1107 6 0.3391755144044737 1107 7 0.3956087798405221 1107 8 0.28739502078416734 1107 9 0.39125792133618376 1107 10-11 0.3981905311441949 1107 12-13 0.4455619528787551 1107 14-15 0.5135833588736674 1107 16-17 0.5987419969866821 1107 18-19 0.6912869281296707 1107 20-21 0.6881836906097547 1107 22-23 0.6775414566372859 1107 24-25 0.8788539509807762 1107 26-27 0.6971290162552464 1107 28-29 0.6851131202034253 1107 30-31 0.7350267410023648 1107 32-33 0.7471786442217905 1107 34-35 0.8695170556467602 1107 36-37 0.8155496700982141 1107 38-39 0.7682925071402522 1107 40-41 0.8414056135781465 1107 42-43 0.9866570549649438 1107 44-45 0.97536537395073 1107 46-47 0.9640643535198024 1107 48-49 1.0578164743461258 1107 50-51 1.2216161092813707 1107 52-53 1.2990995220706552 1107 54-55 1.3183557847676255 1107 56-57 1.3807936319779728 1107 58-59 1.6259417509704193 1107 60-61 1.720447116864765 1107 62-63 1.87164053862233 1107 64-65 2.032708263055337 1107 66-67 2.160140578015799 1107 68-69 2.3198667524608823 1107 70-71 2.2281408866538257 1107 72-73 2.5946549860844037 1107 74-75 2.7568930109373255 1107 76-77 2.807346545380369 1107 78-79 3.117747820482336 1107 80-81 3.383952301803486 1107 82-83 3.5450700040123593 1107 84-85 3.6629442908783254 1107 86-87 3.9462903061782626 1107 88-89 4.069959340436689 1107 90-91 4.355397398615139 1107 92-93 4.581070503642927 1107 94-95 4.681972586054744 1107 96-97 4.7507432324563545 1107 98-99 4.955990084243144 1107 100-101 4.8658640419767565 1108 1 0.3981230309171053 1108 2 0.5026260759060008 1108 3 0.4462684991442991 1108 4 0.3763045459280363 1108 5 0.3477009735720813 1108 6 0.38728354066392257 1108 7 0.3723773157586834 1108 8 0.3711921357058614 1108 9 0.3089643295645601 1108 10-11 0.39125266548980875 1108 12-13 0.45914461989004707 1108 14-15 0.376724689217788 1108 16-17 0.658275093631481 1108 18-19 0.7004646869137048 1108 20-21 0.6065029200648908 1108 22-23 0.6936276151834733 1108 24-25 0.7166469182141526 1108 26-27 0.6597451188037695 1108 28-29 0.6577112270649081 1108 30-31 0.8123408049825898 1108 32-33 0.9559978758955268 1108 34-35 0.9776710237387363 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2311 22-23 0.04821698749439207 2311 24-25 0.07516695228444803 2311 26-27 0.18788113747406499 2311 28-29 0.18441074267066426 2311 30-31 0.3211957314347913 2311 32-33 0.26042065650717916 2311 34-35 0.2057680775881181 2311 36-37 0.2883131376281014 2311 38-39 0.312019582509798 2311 40-41 0.2539923404933191 2311 42-43 0.43692358831889777 2311 44-45 0.44742356384400495 2311 46-47 0.3504225412034856 2311 48-49 0.5073295330994796 2311 50-51 0.5867833762234937 2311 52-53 0.7123934600250053 2311 54-55 0.6346739786381974 2311 56-57 0.77435774913112 2311 58-59 0.8949502745137394 2311 60-61 0.8663418127129212 2311 62-63 1.1312755122175702 2311 64-65 1.1895681134652172 2311 66-67 1.2342987192978114 2311 68-69 1.0628473963391762 2311 70-71 1.432193298435429 2311 72-73 1.4212779394396868 2311 74-75 1.4774852673838446 2311 76-77 1.7197130025030205 2311 78-79 1.8608718123509966 2311 80-81 1.9295202526177242 2311 82-83 1.9777047345512813 2311 84-85 2.3158441185292986 2311 86-87 2.6172728337430584 2311 88-89 2.7915728477394275 2311 90-91 2.9534463192241027 2311 92-93 3.2318419312673825 2311 94-95 3.179385555319165 2311 96-97 3.1277788291596273 2311 98-99 3.4352713280286373 2311 100-101 3.370705143905738 2312 1 -0.3315291188821732 2312 2 -0.33144524229429706 2312 3 -0.27471800190776463 2312 4 -0.18966796108851014 2312 5 -0.2666449296792379 2312 6 -0.15646053084417844 2312 7 -0.231495653604469 2312 8 -0.2029141645529009 2312 9 -0.23159601637182448 2312 10-11 -0.20143382973056845 2312 12-13 -0.25241171537905416 2312 14-15 -0.2000109220301809 2312 16-17 -0.06994809012371661 2312 18-19 0.0441403135072278 2312 20-21 -0.1282474465706258 2312 22-23 -0.17604839201872835 2312 24-25 -0.35092708128402705 2312 26-27 -0.24368081718583312 2312 28-29 -0.1623047757386047 2312 30-31 -0.10004357637813399 2312 32-33 0.04993919454970097 2312 34-35 -0.09594066032805415 2312 36-37 -0.050518833570571076 2312 38-39 -0.041551477192065533 2312 40-41 0.23000777131284167 2312 42-43 0.15479746444043485 2312 44-45 0.14882801791035405 2312 46-47 0.08675585709934808 2312 48-49 0.45665470301420896 2312 50-51 0.5122009700940922 2312 52-53 0.6751311964536271 2312 54-55 0.6455292731235218 2312 56-57 0.6935759871150253 2312 58-59 0.6637178209678254 2312 60-61 0.692572998688906 2312 62-63 0.7762326629886473 2312 64-65 0.906745698000055 2312 66-67 1.1492308271794158 2312 68-69 1.3249005779396974 2312 70-71 1.3308411567787886 2312 72-73 1.3170115270918146 2312 74-75 1.2940239698482934 2312 76-77 1.5296918629241247 2312 78-79 1.6883863961118806 2312 80-81 1.940674666779092 2312 82-83 2.178722546256118 2312 84-85 2.2139958137867097 2312 86-87 2.6206828651170895 2312 88-89 2.592723653685873 2312 90-91 2.770684599567648 2312 92-93 3.005860101111022 2312 94-95 3.250417960844967 2312 96-97 3.427540673561058 2312 98-99 3.500943321171686 2312 100-101 3.1675814223740844 2313 1 -0.4655249748271295 2313 2 -0.5583880969672599 2313 3 -0.44228752275341066 2313 4 -0.3207037010128104 2313 5 -0.38580651292518553 2313 6 -0.29839535567221276 2313 7 -0.2206894582475556 2313 8 -0.11946445081915869 2313 9 -0.3197762510870632 2313 10-11 -0.18968504709371103 2313 12-13 -0.09971888500234627 2313 14-15 -0.26866998893589056 2313 16-17 -0.19025119462123996 2313 18-19 -0.2110292850864326 2313 20-21 -0.10358460072485087 2313 22-23 -0.18562125044486066 2313 24-25 -0.05956150024380236 2313 26-27 -0.09165687208623297 2313 28-29 -0.10117821883194722 2313 30-31 -0.04757017742075931 2313 32-33 0.09964201511662907 2313 34-35 0.12618005903436824 2313 36-37 0.06547973979633781 2313 38-39 0.17594443003006432 2313 40-41 0.12644760492779739 2313 42-43 0.19984607574171065 2313 44-45 0.12258994253880928 2313 46-47 0.3640109521983703 2313 48-49 0.47477299704667075 2313 50-51 0.6196251134296418 2313 52-53 0.60058397930095 2313 54-55 0.5339451009653686 2313 56-57 0.6895679776735832 2313 58-59 0.7938698349796525 2313 60-61 0.8138107035761344 2313 62-63 0.9695860803306431 2313 64-65 1.0619029202697092 2313 66-67 1.3067808277968886 2313 68-69 1.5588680703568478 2313 70-71 1.5783153530828606 2313 72-73 1.5630365390788619 2313 74-75 1.6276267097140718 2313 76-77 1.815193388621509 2313 78-79 2.017539632721963 2313 80-81 2.2398530372601932 2313 82-83 2.4717963150534956 2313 84-85 2.629786271614048 2313 86-87 2.5850803958659476 2313 88-89 2.6029183485651863 2313 90-91 2.657598019529882 2313 92-93 2.8809156066422474 2313 94-95 2.9531244293401073 2313 96-97 3.038240247289316 2313 98-99 3.0782368204605177 2313 100-101 3.0680165132528145 2314 1 -0.4121304911104531 2314 2 -0.42555306672177906 2314 3 -0.5604152483635971 2314 4 -0.46205186694092504 2314 5 -0.4166954794793085 2314 6 -0.44013862517351043 2314 7 -0.3914176138007619 2314 8 -0.3120222161704973 2314 9 -0.5480576439617906 2314 10-11 -0.48234304456153865 2314 12-13 -0.5141347251936637 2314 14-15 -0.5922106334987305 2314 16-17 -0.4932046899964888 2314 18-19 -0.4571873287137862 2314 20-21 -0.4120533353383493 2314 22-23 -0.5928073546686718 2314 24-25 -0.49097558637308225 2314 26-27 -0.5444024297812433 2314 28-29 -0.4850922280286909 2314 30-31 -0.4676059400640682 2314 32-33 -0.44764265068839393 2314 34-35 -0.42100569349780415 2314 36-37 -0.2891650076890713 2314 38-39 -0.16581026944943034 2314 40-41 -0.19850117997760464 2314 42-43 0.04590240355348385 2314 44-45 -0.11271181987590495 2314 46-47 -0.13292136254806053 2314 48-49 -0.03891082997380124 2314 50-51 0.003743818149345657 2314 52-53 0.10057024134638937 2314 54-55 0.015063930975216522 2314 56-57 0.11505056454141283 2314 58-59 0.5567865346174088 2314 60-61 0.542384714883017 2314 62-63 0.5680668823634178 2314 64-65 0.5344748192287021 2314 66-67 0.8223184544058775 2314 68-69 1.0566955502225035 2314 70-71 1.3122839843869336 2314 72-73 1.284821528000574 2314 74-75 1.4125348522546908 2314 76-77 1.5765469837892638 2314 78-79 1.8057457086346034 2314 80-81 2.027248299094616 2314 82-83 2.0973103893131864 2314 84-85 2.3994411632051005 2314 86-87 2.658958719351915 2314 88-89 2.734973062991582 2314 90-91 2.871701843297206 2314 92-93 2.8767848784647114 2314 94-95 3.003654000012542 2314 96-97 3.0278252291771963 2314 98-99 3.3062371347849187 2314 100-101 3.170746464631442 2315 1 -0.5496636455639425 2315 2 -0.5620453255424067 2315 3 -0.4707324516697895 2315 4 -0.37554572951186316 2315 5 -0.4137520579397105 2315 6 -0.2099060042754104 2315 7 -0.1899252778767604 2315 8 -0.42089989936812344 2315 9 -0.2752485365105173 2315 10-11 -0.48102457675989996 2315 12-13 -0.3388324799432354 2315 14-15 -0.4370834514730788 2315 16-17 -0.32868631626098477 2315 18-19 -0.38229199655242496 2315 20-21 -0.2746242704481361 2315 22-23 -0.3664520700029996 2315 24-25 -0.3600212778361964 2315 26-27 -0.4351594693198564 2315 28-29 -0.37980081580317204 2315 30-31 -0.35033362634583654 2315 32-33 -0.3202803958771483 2315 34-35 -0.49300964890120724 2315 36-37 -0.09593237137028865 2315 38-39 -0.1914837674755816 2315 40-41 -0.1703468806931241 2315 42-43 -0.15949521857826454 2315 44-45 -0.12145052423070268 2315 46-47 -0.014792237517212925 2315 48-49 -0.03362250188349947 2315 50-51 0.11148422648884804 2315 52-53 0.3152356897796551 2315 54-55 0.24474237132803722 2315 56-57 0.5415883914597472 2315 58-59 0.7134538566628024 2315 60-61 0.7432785358181242 2315 62-63 0.879639714216367 2315 64-65 0.8291427349701266 2315 66-67 1.007476439231933 2315 68-69 1.2273166565018023 2315 70-71 1.0610956285394089 2315 72-73 1.3176617852174495 2315 74-75 1.492782122795031 2315 76-77 1.604518162545741 2315 78-79 1.9350856651482964 2315 80-81 2.1006989066692547 2315 82-83 2.2769275324787444 2315 84-85 2.3993457477720916 2315 86-87 2.531518397368199 2315 88-89 2.7634434861349284 2315 90-91 2.9219404782589606 2315 92-93 2.851851572888595 2315 94-95 2.918889435184017 2315 96-97 3.0380741959919 2315 98-99 3.2593284020560738 2315 100-101 3.251081440266777 2316 1 -0.7673897096089846 2316 2 -0.7792094025165355 2316 3 -0.7923625526332181 2316 4 -0.5187388285048797 2316 5 -0.5200152927425847 2316 6 -0.44034645557206886 2316 7 -0.501863125255845 2316 8 -0.5462614237819707 2316 9 -0.6270274264572961 2316 10-11 -0.6613209627665881 2316 12-13 -0.5878164444022218 2316 14-15 -1.0297874789735104 2316 16-17 -0.7716612571412469 2316 18-19 -0.6703802692846423 2316 20-21 -0.5544323943178 2316 22-23 -0.8225822026048704 2316 24-25 -0.782428801605505 2316 26-27 -0.6049302598247976 2316 28-29 -0.50924870287011 2316 30-31 -0.3329843541036439 2316 32-33 -0.3727342883623521 2316 34-35 -0.4548182201237694 2316 36-37 -0.43721450830442876 2316 38-39 -0.38228360207273937 2316 40-41 -0.4180301371867259 2316 42-43 -0.4981959527373405 2316 44-45 -0.37407585293943413 2316 46-47 -0.20733167152751264 2316 48-49 -0.05734331541827942 2316 50-51 0.09958809771007537 2316 52-53 0.023106304728003124 2316 54-55 -0.025741755737847427 2316 56-57 0.18497669229868308 2316 58-59 0.3503107316968439 2316 60-61 0.2657299855210944 2316 62-63 0.4771559491531576 2316 64-65 0.5189156445150793 2316 66-67 0.7812971713896886 2316 68-69 0.9444929473871362 2316 70-71 0.7960525781813068 2316 72-73 1.1834143460920252 2316 74-75 1.3011810997696038 2316 76-77 1.4622324247151397 2316 78-79 1.7110724535224193 2316 80-81 2.0373393894572054 2316 82-83 2.1007663647699673 2316 84-85 1.9397592990329926 2316 86-87 2.330187596091477 2316 88-89 2.338114630254669 2316 90-91 2.662262793074728 2316 92-93 2.6733618156511163 2316 94-95 2.70504158225717 2316 96-97 2.7471141089935394 2316 98-99 2.7740104798336738 2316 100-101 2.812005680171538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8308.0 3 2576.0 4 2648.0 5 3125.0 6 3591.0 7 4651.0 8 5663.0 9 7357.0 10 9551.0 11 12180.0 12 15212.0 13 18786.0 14 22230.0 15 26887.0 16 31431.0 17 37278.0 18 35194.0 19 31861.0 20 33774.0 21 36637.0 22 40911.0 23 45107.0 24 51211.0 25 56874.0 26 64394.0 27 72768.0 28 81368.0 29 92174.0 30 104066.0 31 119708.0 32 137890.0 33 161192.0 34 191748.0 35 230299.0 36 268205.0 37 285219.0 38 173238.0 39 15227.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41505417353139 19.350154507972945 15.63754131168524 22.597250006810434 2 15.072298507016043 20.493219812425114 40.6438372723482 23.790644408210643 3 18.826288694909803 24.70831270878053 30.193951522554695 26.271447073754977 4 12.598202563288721 17.34937548092183 37.80126282097225 32.2511591348172 5 13.49563159774859 37.59299322693833 34.41067431683529 14.500700858477789 6 31.01297754028134 36.83717599964397 17.861476543266278 14.288369916808419 7 27.895194821557943 33.248023435285226 20.88984785964028 17.966933883516553 8 26.175147251469955 34.73437642117996 20.181493662841678 18.908982664508407 9 26.510984683816258 16.883635435764642 20.389078949855406 36.21630093056369 10-11 24.48788788981914 27.069400293690148 27.568110502149523 20.874601314341188 12-13 25.80827683829935 24.40042091958526 27.776440118873364 22.014862123242025 14-15 22.49480725061564 25.797102533447486 26.608413641885637 25.099676574051234 16-17 21.619266346707864 28.930956890206726 27.330493585764966 22.119283177320444 18-19 21.64446847878319 27.243266376876583 29.935554156104345 21.176710988235882 20-21 22.38047170065785 26.430904935922843 30.10081882439663 21.087804539022674 22-23 22.047222857133857 26.39462524168477 29.980291161616606 21.577860739564763 24-25 21.754764522775822 26.564026080819662 30.13942668195767 21.541782714446846 26-27 21.42877817199044 26.83669376711909 29.922605108846472 21.811922952043993 28-29 21.45240129226067 27.211068914728237 29.843964387031637 21.49256540597945 30-31 21.660327342624534 26.768078788094762 29.78287872580079 21.788715143479916 32-33 21.348089196677865 26.819876906285057 29.668038355847226 22.163995541189855 34-35 22.136162781884206 26.951324310676284 29.399706857160297 21.512806050279217 36-37 22.136054137846447 26.53030488565951 29.496397070722622 21.83724390577142 38-39 21.619959071800725 26.908775311429896 29.41275356704453 22.05851204972485 40-41 22.13554928058156 26.643885164695103 29.458194598776217 21.762370955947123 42-43 21.792049813369154 26.753820115502077 29.241358702477637 22.212771368651136 44-45 21.813355619401868 27.02000136597959 29.2931567374975 21.873486277121042 46-47 22.169002501280954 27.27705244501345 28.640601929413368 21.91334312429223 48-49 22.067090129971874 27.22425511728569 28.51086445171869 22.197790301023755 50-51 22.160035427840377 27.41333464547557 28.45052403680559 21.976105889878465 52-53 22.329752751075564 27.331375517115212 28.435604333228166 21.903267398581058 54-55 21.92502780600531 27.414113346154306 28.549125845325197 22.111733002515184 56-57 22.053552537442396 27.473658209673467 28.380483014010576 22.09230623887356 58-59 22.086451662680346 27.15780780331575 28.352640338598214 22.403100195405685 60-61 21.779345351911974 27.37446968899246 28.696933225343923 22.149251733751647 62-63 21.888702174870602 27.391438525885608 28.71777067398512 22.00208862525867 64-65 21.664531763346165 27.26863329709115 28.603800783996334 22.46303415556635 66-67 21.57395997612242 27.38409228097999 28.55779773611732 22.484150006780272 68-69 22.034612539763206 27.13780497261993 28.58955317591939 22.238029311697478 70-71 21.92779854168848 27.171779522698625 28.25185280832439 22.648569127288503 72-73 21.932895544386657 27.273403671931497 28.217014560727566 22.576686222954276 74-75 21.998856017207768 27.607855610948224 28.015178045608145 22.378110326235863 76-77 22.07696324495171 27.207222953437803 28.199058336044637 22.516755465565847 78-79 22.0747885461636 27.364942928031887 28.08603328072984 22.474235245074674 80-81 22.212663173841563 27.530669488324545 27.816889834690134 22.43977750314376 82-83 22.298534555196728 27.431163893972112 27.87254324775605 22.39775830307511 84-85 22.24524331371456 27.453960441249176 27.903039020258035 22.397757224778225 86-87 22.282385357213148 27.53524896673883 27.70980122023224 22.472564455815785 88-89 22.266237147139964 27.432616909723144 27.61420800201571 22.68693794112118 90-91 22.23045121663123 27.47541144972045 27.64276714701945 22.65137018662887 92-93 22.33484233435812 27.09940530308324 27.84005520821872 22.725697154339922 94-95 22.1773528375391 26.92395367652704 27.966133391601854 22.932560094332008 96-97 22.3502782198027 26.748135293483116 27.918473369715418 22.98311311699877 98-99 22.28425343825123 26.819333924754602 27.54758123201304 23.348831404981127 100-101 22.702568410699833 26.317343845184517 27.937761098637658 23.042326645477996 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1073.0 1 695.0 2 457.0 3 615.5 4 547.0 5 531.5 6 946.0 7 2235.5 8 3669.5 9 4125.5 10 3398.5 11 2595.0 12 2518.5 13 2703.0 14 2871.5 15 2946.0 16 3214.5 17 3547.0 18 3883.0 19 4403.0 20 4956.5 21 5855.0 22 7147.5 23 8581.5 24 10485.0 25 12995.0 26 16198.5 27 20000.5 28 23828.5 29 27938.5 30 32537.5 31 38528.5 32 46175.5 33 51024.0 34 53399.0 35 57053.5 36 61896.0 37 66644.0 38 70265.0 39 73334.0 40 76834.5 41 81278.0 42 85176.0 43 87866.5 44 90111.5 45 93760.5 46 95547.5 47 96230.0 48 96925.0 49 93161.0 50 88220.5 51 83804.5 52 79876.5 53 78371.5 54 77945.5 55 73912.5 56 66937.0 57 60769.0 58 56121.0 59 49450.5 60 40206.5 61 33241.5 62 28040.0 63 21965.0 64 16423.5 65 12676.5 66 9795.5 67 7487.5 68 5664.0 69 4266.0 70 3245.0 71 2488.5 72 1958.0 73 1553.5 74 1134.5 75 820.5 76 622.0 77 443.5 78 310.5 79 212.0 80 137.0 81 93.0 82 65.5 83 37.5 84 18.0 85 10.5 86 8.5 87 7.0 88 5.0 89 3.0 90 2.5 91 3.0 92 4.0 93 3.5 94 2.0 95 0.5 96 0.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30151082112890215 2 0.0338510843565086 3 0.04290428133557485 4 0.04506917626535157 5 0.05943620625386975 6 0.05746811995407274 7 0.030662784550837437 8 0.032906402932606035 9 0.03947981117392805 10-11 0.030997359221802936 12-13 0.03391012694550251 14-15 0.027533527334160195 16-17 0.02064522528487065 18-19 0.02576224966434288 20-21 0.038712257517007215 22-23 0.03209948754968926 24-25 0.053335138724499014 26-27 0.0456399212922927 28-29 0.037728214367108714 30-31 0.038082469901072176 32-33 0.03337874364455731 34-35 0.0323356579056649 36-37 0.03148938079675218 38-39 0.01893299020404725 40-41 0.01836224517710612 42-43 0.019602139545978236 44-45 0.009348409924035805 46-47 0.01733884030121167 48-49 0.014721285522481647 50-51 0.006455323063334198 52-53 0.004861173160498619 54-55 0.00671117428230781 56-57 0.0053728755984458415 58-59 0.007203195857257062 60-61 0.006553727378324048 62-63 0.004408513311545305 64-65 0.008698941445102791 66-67 0.0031686189426731884 68-69 0.007400004487236763 70-71 0.01342234856461562 72-73 0.013776604098579082 74-75 0.011454262264818608 76-77 0.009781388909991147 78-79 0.005904258899391035 80-81 0.007419685350234733 82-83 0.012615433181698846 84-85 0.0045265984895331264 86-87 0.0015351073138416692 88-89 0.01934628832700462 90-91 0.029088315510999833 92-93 0.013284582523629827 94-95 0.022593630721669693 96-97 0.018145755684128448 98-99 0.013009050441658248 100-101 0.10647346881901834 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2540539.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.93027902873953 #Duplication Level Percentage of deduplicated Percentage of total 1 75.55363777814779 42.25736042568121 2 13.719047061646302 15.346202603325738 3 4.737346021528153 7.948832545192761 4 2.127227367866369 4.759056809693487 5 1.1195415758939566 3.1308136362011885 6 0.6592131529560015 2.2121985350546587 7 0.4310880508607647 1.6877612479418616 8 0.29500080293649766 1.3199581777552414 9 0.21116017575312893 1.062922279466716 >10 1.0397885447508548 10.416514774175054 >50 0.06907252196584099 2.625658660383413 >100 0.032920528204872256 3.3607165687278 >500 0.002761432601265259 1.0976082922508388 >1k 0.0019825669957748986 1.7092211680420268 >5k 1.4161192826963562E-4 0.6063062113698663 >10k+ 7.080596413481781E-5 0.4588680647382522 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11587 0.45608431911495945 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8885 0.3497289354739289 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6425 0.252899089523916 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.936172599594023E-5 2 0.0 0.0 0.0 0.0 3.936172599594023E-5 3 0.0 0.0 0.0 0.0 3.936172599594023E-5 4 0.0 0.0 0.0 0.0 3.936172599594023E-5 5 0.0 0.0 0.0 0.0 3.936172599594023E-5 6 0.0 0.0 0.0 0.0 3.936172599594023E-5 7 0.0 0.0 0.0 0.0 3.936172599594023E-5 8 0.0 0.0 0.0 0.0 3.936172599594023E-5 9 0.0 0.0 0.0 0.0 3.936172599594023E-5 10-11 0.0 0.0 0.0 3.936172599594023E-5 3.936172599594023E-5 12-13 0.0 0.0 0.0 3.936172599594023E-5 3.936172599594023E-5 14-15 7.872345199188046E-5 0.0 0.0 1.180851779878207E-4 7.872345199188046E-5 16-17 1.180851779878207E-4 0.0 0.0 1.180851779878207E-4 7.872345199188046E-5 18-19 1.3776604098579082E-4 0.0 0.0 1.3776604098579082E-4 7.872345199188046E-5 20-21 1.5744690398376092E-4 0.0 0.0 2.361703559756414E-4 7.872345199188046E-5 22-23 1.5744690398376092E-4 0.0 0.0 4.1329812295737246E-4 7.872345199188046E-5 24-25 1.9680862997970117E-4 0.0 0.0 8.856388349086552E-4 1.180851779878207E-4 26-27 1.9680862997970117E-4 0.0 0.0 0.0017515968068193403 1.180851779878207E-4 28-29 1.9680862997970117E-4 0.0 0.0 0.004861173160498619 1.180851779878207E-4 30-31 1.9680862997970117E-4 0.0 0.0 0.012851603537674484 1.180851779878207E-4 32-33 1.9680862997970117E-4 0.0 0.0 0.02698246317021703 1.180851779878207E-4 34-35 2.361703559756414E-4 0.0 0.0 0.049064391453939496 1.180851779878207E-4 36-37 3.542555339634621E-4 0.0 0.0 0.08547398800018421 1.180851779878207E-4 38-39 4.3297898595534253E-4 0.0 0.0 0.14213519257134016 1.180851779878207E-4 40-41 4.3297898595534253E-4 0.0 0.0 0.20468097517888922 1.180851779878207E-4 42-43 4.3297898595534253E-4 0.0 0.0 0.27114324952303426 1.180851779878207E-4 44-45 4.723407119512828E-4 0.0 0.0 0.3565581949342246 1.180851779878207E-4 46-47 5.11702437947223E-4 0.0 0.0 0.45720612830584373 1.180851779878207E-4 48-49 5.11702437947223E-4 0.0 0.0 0.5635418310838762 1.180851779878207E-4 50-51 5.11702437947223E-4 0.0 0.0 0.6770413679931699 1.180851779878207E-4 52-53 5.11702437947223E-4 0.0 0.0 0.7864866471248817 1.180851779878207E-4 54-55 5.11702437947223E-4 0.0 0.0 0.9091377853282315 1.180851779878207E-4 56-57 5.904258899391034E-4 0.0 0.0 1.0526506383094296 1.180851779878207E-4 58-59 5.904258899391034E-4 0.0 0.0 1.2158049925626018 1.180851779878207E-4 60-61 5.904258899391034E-4 0.0 0.0 1.393503504571274 1.180851779878207E-4 62-63 5.904258899391034E-4 0.0 0.0 1.5705722289640112 1.180851779878207E-4 64-65 5.904258899391034E-4 0.0 0.0 1.7563595756648491 1.180851779878207E-4 66-67 6.297876159350437E-4 0.0 0.0 1.973636303162439 1.180851779878207E-4 68-69 6.297876159350437E-4 0.0 0.0 2.2143923002166073 1.180851779878207E-4 70-71 6.297876159350437E-4 0.0 0.0 2.467173304562536 1.180851779878207E-4 72-73 6.691493419309839E-4 0.0 0.0 2.7325697420901625 1.7712776698173104E-4 74-75 7.085110679269242E-4 0.0 0.0 3.0009379899304833 2.361703559756414E-4 76-77 7.085110679269242E-4 0.0 0.0 3.296642956474984 2.361703559756414E-4 78-79 7.281919309248942E-4 0.0 0.0 3.6285213492097546 2.361703559756414E-4 80-81 8.659579719106851E-4 0.0 0.0 3.976085389753907 2.361703559756414E-4 82-83 8.659579719106851E-4 0.0 0.0 4.331167519963284 2.558512189736115E-4 84-85 8.659579719106851E-4 0.0 0.0 4.696601784109593 2.7553208197158164E-4 86-87 8.659579719106851E-4 0.0 0.0 5.07319509757575 2.7553208197158164E-4 88-89 8.659579719106851E-4 0.0 0.0 5.480726727674718 2.7553208197158164E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6575 0.0 45.364685 1 GTATCAA 10830 0.0 39.4642 1 TATCAAC 13550 0.0 31.460838 2 ATCAACG 13785 0.0 31.027939 3 TCAACGC 13875 0.0 30.758171 4 CAACGCA 14215 0.0 30.022486 5 AACGCAG 14675 0.0 29.142729 6 ACGCAGA 16375 0.0 25.908987 7 CGCAGAG 16600 0.0 25.41471 8 GCAGAGT 17495 0.0 23.46189 9 GTACATG 10330 0.0 22.924263 1 GAGTACT 10100 0.0 22.714066 12-13 TACATGG 10335 0.0 22.210268 2 AGTACTT 10570 0.0 21.209778 12-13 GTACTTT 10875 0.0 20.856585 14-15 CAGAGTA 16945 0.0 20.448496 10-11 AGAGTAC 15765 0.0 20.337029 10-11 ACATGGG 10960 0.0 20.24993 3 GTAAGGT 1525 0.0 19.321417 4 TAAGGTG 1635 0.0 18.893515 5 >>END_MODULE