FastQCFastQC Report
Thu 26 May 2016
SRR938321_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938321_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2540539
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105540.4154236561611532No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92520.364174688914439No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66910.26336930863883606No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28590.11253517462239312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62450.045.2442741
GTATCAA101500.040.2097051
TATCAAC131900.030.7258872
ATCAACG132650.030.5383173
TCAACGC134500.030.1182734
CAACGCA136650.029.5748185
AACGCAG141900.028.5842276
TAAGGTG15100.025.819615
ACGCAGA157900.025.4772327
CGCAGAG159050.025.113858
GCAGAGT171650.022.8829869
GGTAAGG15950.022.356993
GTACATG99450.022.2411771
GAGTACT101950.021.75607712-13
TACATGG98650.021.553612
GTAAGGT16550.021.259184
AGTACTT105500.021.24909812-13
AGAGTAC156150.020.95695510-11
GTACTTT105050.020.79757714-15
AGGTAAG16250.020.7833042
Produced by FastQC (version 0.11.5)