##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938321_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2540539 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.76391191003169 31.0 31.0 34.0 30.0 34.0 2 32.00518039675833 31.0 31.0 34.0 30.0 34.0 3 32.06174280339723 33.0 31.0 34.0 30.0 34.0 4 35.74118838561424 37.0 35.0 37.0 33.0 37.0 5 35.61494903246909 37.0 35.0 37.0 33.0 37.0 6 35.65564826991438 37.0 35.0 37.0 33.0 37.0 7 35.59235933792002 37.0 35.0 37.0 33.0 37.0 8 35.57733417987286 37.0 35.0 37.0 33.0 37.0 9 37.138883914004076 39.0 37.0 39.0 33.0 39.0 10-11 37.07226183105239 39.0 37.0 39.0 33.0 39.0 12-13 36.96630281999214 39.0 37.0 39.0 33.0 39.0 14-15 37.98255350537819 40.0 37.0 41.0 33.0 41.0 16-17 37.90484046889263 40.0 37.0 41.0 33.0 41.0 18-19 37.93227992170165 40.0 37.0 41.0 33.0 41.0 20-21 37.934452492168 40.0 37.0 41.0 32.5 41.0 22-23 37.959869736303986 40.0 37.0 41.0 33.0 41.0 24-25 37.882829391715696 40.0 37.0 41.0 32.0 41.0 26-27 37.69882513120247 40.0 37.0 41.0 32.0 41.0 28-29 37.56597655064535 39.5 36.5 41.0 32.0 41.0 30-31 37.29841364371891 39.0 36.0 41.0 31.5 41.0 32-33 37.05438845851215 39.0 36.0 40.5 31.0 41.0 34-35 36.86688867992186 39.0 35.0 40.5 30.0 41.0 36-37 36.84542158179819 39.0 35.0 41.0 30.0 41.0 38-39 36.684400239476744 39.0 35.0 41.0 30.0 41.0 40-41 36.49800967432502 39.0 35.0 40.0 30.0 41.0 42-43 36.25278159477182 39.0 35.0 40.0 30.0 41.0 44-45 35.93931661745795 38.0 35.0 40.0 29.0 41.0 46-47 35.541345163368874 38.0 34.5 40.0 27.5 41.0 48-49 35.1143168831496 38.0 34.0 40.0 26.5 41.0 50-51 34.73783634102842 38.0 34.0 40.0 25.5 41.0 52-53 34.21125280895117 38.0 33.0 40.0 24.0 41.0 54-55 33.8868509792607 37.5 33.0 40.0 23.0 41.0 56-57 33.51523042944824 37.0 32.0 40.0 22.5 41.0 58-59 33.056298092648845 36.5 31.5 39.0 20.5 41.0 60-61 32.64275710784207 36.0 31.0 39.0 19.0 40.0 62-63 32.161000283798046 35.5 31.0 39.0 17.5 40.0 64-65 31.661491124521213 35.0 30.0 38.5 13.5 40.0 66-67 31.154986796109014 35.0 29.5 38.0 10.0 40.0 68-69 30.58317978980051 34.0 29.0 38.0 8.0 40.0 70-71 30.004126486544784 34.0 28.0 37.0 7.0 39.0 72-73 29.41068824371521 34.0 28.0 36.0 2.0 39.0 74-75 28.783314485626867 33.5 26.5 36.0 2.0 38.5 76-77 27.518248096171718 31.5 26.0 34.5 2.0 36.5 78-79 28.121067812775163 33.0 26.0 35.0 2.0 37.0 80-81 27.799915687182917 33.0 26.0 35.0 2.0 37.0 82-83 27.276060709951707 33.0 25.5 35.0 2.0 36.0 84-85 26.65745497313759 32.0 25.0 35.0 2.0 36.0 86-87 25.98391227215957 32.0 23.0 34.5 2.0 35.5 88-89 25.215382247625406 31.0 20.0 34.0 2.0 35.0 90-91 24.636282891150266 31.0 18.5 34.0 2.0 35.0 92-93 23.831272222154432 31.0 9.0 34.0 2.0 35.0 94-95 23.017588196835398 30.0 2.0 34.0 2.0 35.0 96-97 22.020072315363002 29.5 2.0 34.0 2.0 35.0 98-99 20.824103467807422 29.0 2.0 34.0 2.0 35.0 100-101 17.745000568776938 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34604400010745806 1101 2 0.34420218465621844 1101 3 0.3102456577672683 1101 4 0.2145788790572425 1101 5 0.18715404848293815 1101 6 0.20670860580079875 1101 7 0.24280629581691215 1101 8 0.22137554958790417 1101 9 0.36832990152629463 1101 10-11 0.34278049857201154 1101 12-13 0.3942151637479654 1101 14-15 0.5157981502342821 1101 16-17 0.5510478649565727 1101 18-19 0.648105333982727 1101 20-21 0.6817545562511853 1101 22-23 0.6453464827732134 1101 24-25 0.6286612981450332 1101 26-27 0.5819123170126872 1101 28-29 0.662464241494277 1101 30-31 0.6795436544926545 1101 32-33 0.6063613800497052 1101 34-35 0.5910984717167764 1101 36-37 0.8200189151170818 1101 38-39 0.9674528770916808 1101 40-41 1.006004567507759 1101 42-43 0.9279778129395098 1101 44-45 1.0742842108941844 1101 46-47 1.1122335187612222 1101 48-49 1.2493637255467362 1101 50-51 1.3546799257144073 1101 52-53 1.4678776622359635 1101 54-55 1.4795310372287531 1101 56-57 1.572690362066119 1101 58-59 1.6225077488548365 1101 60-61 1.5112404483308453 1101 62-63 1.6419070580226958 1101 64-65 1.6589858737125773 1101 66-67 1.71366731769346 1101 68-69 1.7664288118203366 1101 70-71 1.8937228603978689 1101 72-73 1.9828588407524066 1101 74-75 2.004213550788606 1101 76-77 1.937358193251825 1101 78-79 2.2333784977330424 1101 80-81 2.4676660154073105 1101 82-83 2.51750913591583 1101 84-85 2.819771972453175 1101 86-87 3.0072864442497824 1101 88-89 3.112751198880332 1101 90-91 3.375392122863694 1101 92-93 3.669028593323212 1101 94-95 3.6821190840881854 1101 96-97 4.231927042827369 1101 98-99 4.410255313269676 1101 100-101 4.788939227850083 1102 1 0.34392584499513745 1102 2 0.3200992875624351 1102 3 0.31033692945570124 1102 4 0.2485104331141983 1102 5 0.17439426643972666 1102 6 0.15394900630096942 1102 7 0.22962079654121936 1102 8 0.19570897288905087 1102 9 0.3424895826490939 1102 10-11 0.3555513636059544 1102 12-13 0.4381054369241255 1102 14-15 0.53374460895796 1102 16-17 0.49235902839679113 1102 18-19 0.6121182111140868 1102 20-21 0.5706816379954134 1102 22-23 0.5282224145593588 1102 24-25 0.5796117297949266 1102 26-27 0.6666293943367521 1102 28-29 0.5452417302450385 1102 30-31 0.5475905446706477 1102 32-33 0.5850355870617179 1102 34-35 0.7269218270987494 1102 36-37 0.8670984818634153 1102 38-39 0.8103451269518374 1102 40-41 0.8393246990432957 1102 42-43 0.7863771226647671 1102 44-45 0.6787823078794801 1102 46-47 0.8455382970948975 1102 48-49 0.8773872632112969 1102 50-51 0.8773832692571233 1102 52-53 1.0691975486418244 1102 54-55 1.0001977182381196 1102 56-57 1.2917283975864535 1102 58-59 1.3146336750994934 1102 60-61 1.3442315128325433 1102 62-63 1.4213055058824438 1102 64-65 1.3570586394142445 1102 66-67 1.4304693598322906 1102 68-69 1.656568014184021 1102 70-71 1.7717113562534799 1102 72-73 1.859025814483111 1102 74-75 1.989827828809993 1102 76-77 1.9890238363717003 1102 78-79 2.301669930841978 1102 80-81 2.479326788530667 1102 82-83 2.4448074874187498 1102 84-85 2.777200901145214 1102 86-87 2.6880687664883887 1102 88-89 2.974832995263629 1102 90-91 3.2451937112518507 1102 92-93 3.435738483653761 1102 94-95 3.576600048073683 1102 96-97 3.9663635900976253 1102 98-99 4.335388732919949 1102 100-101 4.758412596727219 1103 1 0.24201801022068636 1103 2 0.32775538033275353 1103 3 0.3107186671255704 1103 4 0.2307058088246876 1103 5 0.15608413611781202 1103 6 0.213005483559364 1103 7 0.2128665436042141 1103 8 0.24774906435434474 1103 9 0.3339609466333542 1103 10-11 0.4335085976059361 1103 12-13 0.45943793375403885 1103 14-15 0.5784287814976992 1103 16-17 0.46767885817497756 1103 18-19 0.4057318584491796 1103 20-21 0.5620171413058586 1103 22-23 0.49063144955498217 1103 24-25 0.4540721993180341 1103 26-27 0.5078869852258876 1103 28-29 0.473322353240917 1103 30-31 0.510635492977201 1103 32-33 0.5627677143885421 1103 34-35 0.6704974429293316 1103 36-37 0.7720486473146835 1103 38-39 0.7390742297782111 1103 40-41 0.6306581158372921 1103 42-43 0.8118866831780096 1103 44-45 0.8893770219745605 1103 46-47 0.9474179164763541 1103 48-49 1.1756581101188388 1103 50-51 1.1542165821995027 1103 52-53 1.2892529551379113 1103 54-55 1.3787332966631496 1103 56-57 1.398340852761912 1103 58-59 1.516412857166074 1103 60-61 1.5614153510554374 1103 62-63 1.6837989973737209 1103 64-65 1.5784868788970208 1103 66-67 1.6284989769849751 1103 68-69 1.6484109203600106 1103 70-71 1.7429145839047528 1103 72-73 1.7891486154607392 1103 74-75 1.8352198046367505 1103 76-77 1.7887320085927705 1103 78-79 2.043683077792487 1103 80-81 2.315775196091824 1103 82-83 2.484330376978484 1103 84-85 2.7971875948607234 1103 86-87 2.8730035960437057 1103 88-89 2.9602598633360415 1103 90-91 3.1417183809894915 1103 92-93 3.5005693952053782 1103 94-95 3.5313816799002673 1103 96-97 4.012459717807609 1103 98-99 4.4392422442900425 1103 100-101 4.746016317934476 1104 1 0.30588894637422825 1104 2 0.31262262335216917 1104 3 0.23194929216054305 1104 4 0.19249795811683157 1104 5 0.20154186092335635 1104 6 0.17102755842925887 1104 7 0.17266523981594872 1104 8 0.15418267187490642 1104 9 0.2822990362757878 1104 10-11 0.22779997749513825 1104 12-13 0.29619685882818914 1104 14-15 0.42576175111327785 1104 16-17 0.4708666344605774 1104 18-19 0.5495787332743092 1104 20-21 0.5627970361074901 1104 22-23 0.5216383881706221 1104 24-25 0.4536840005602656 1104 26-27 0.5547173948280388 1104 28-29 0.5423675241882577 1104 30-31 0.5997725726034133 1104 32-33 0.6068405138961168 1104 34-35 0.6638910694751345 1104 36-37 0.5124328591662888 1104 38-39 0.5656088389215128 1104 40-41 0.6189177263795074 1104 42-43 0.7477272209207229 1104 44-45 0.6889248228810558 1104 46-47 0.7752622592663485 1104 48-49 0.8320952149255092 1104 50-51 0.9340449353974236 1104 52-53 1.094055428418642 1104 54-55 0.9110534662115839 1104 56-57 1.1168722723802844 1104 58-59 1.1884635957929035 1104 60-61 1.2111988690454822 1104 62-63 1.2106056953760742 1104 64-65 1.2601953731055069 1104 66-67 1.4280590183928474 1104 68-69 1.4029766273713626 1104 70-71 1.5099009025215118 1104 72-73 1.711153889368184 1104 74-75 1.9254515348141332 1104 76-77 1.7577670374104066 1104 78-79 2.0706489023126586 1104 80-81 2.3796496295314746 1104 82-83 2.5806945983427063 1104 84-85 2.7535624479747014 1104 86-87 2.8354812134126277 1104 88-89 2.8322521972848946 1104 90-91 3.0387915022473955 1104 92-93 3.1516976197031674 1104 94-95 3.4156949528004965 1104 96-97 3.604865011299861 1104 98-99 3.81496443387093 1104 100-101 4.067180844262047 1105 1 0.26952419357253987 1105 2 0.2851937272104266 1105 3 0.20023667996797911 1105 4 0.16194057547150464 1105 5 0.07330305520174818 1105 6 0.20351656813735275 1105 7 0.244348794342784 1105 8 0.21481869338923332 1105 9 0.25087262960168744 1105 10-11 0.23778959083669093 1105 12-13 0.32242525291341195 1105 14-15 0.4003498233161622 1105 16-17 0.44080724941736804 1105 18-19 0.4543534739200723 1105 20-21 0.42529661980855593 1105 22-23 0.2777642270129732 1105 24-25 0.17499371864263225 1105 26-27 0.40380122494476467 1105 28-29 0.43985859457608 1105 30-31 0.4428002939495599 1105 32-33 0.38563799278976774 1105 34-35 0.4033326877123571 1105 36-37 0.5052980157779459 1105 38-39 0.4465306912852611 1105 40-41 0.4819876396228153 1105 42-43 0.6556340906857656 1105 44-45 0.4618269884681254 1105 46-47 0.5049848584703867 1105 48-49 0.43850850818313347 1105 50-51 0.44745080188202735 1105 52-53 0.6049978459498249 1105 54-55 0.6912554336403574 1105 56-57 0.8250446367917235 1105 58-59 1.0402377577831388 1105 60-61 1.0215814685825606 1105 62-63 1.0469554614160685 1105 64-65 1.110112321467792 1105 66-67 1.1774335292416005 1105 68-69 1.2137224477300528 1105 70-71 1.2407147253113777 1105 72-73 1.3321766734089486 1105 74-75 1.5085307523386504 1105 76-77 1.4229398847286099 1105 78-79 1.7195012702210057 1105 80-81 1.900522858179503 1105 82-83 2.0203083561899398 1105 84-85 2.285532124760209 1105 86-87 2.461348155946723 1105 88-89 2.7336288978660477 1105 90-91 2.897074498101059 1105 92-93 3.0310469028363975 1105 94-95 2.9827159592448034 1105 96-97 3.3624437334286448 1105 98-99 3.7623968061087822 1105 100-101 4.219507226790814 1106 1 0.19818274553823656 1106 2 0.1832107929582918 1106 3 0.12090887247096305 1106 4 0.11535043436060022 1106 5 0.1480339076976378 1106 6 0.08056255450319583 1106 7 0.15918995288387805 1106 8 0.1097375874526989 1106 9 0.21553590275657797 1106 10-11 0.1764803074258836 1106 12-13 0.1082908810962735 1106 14-15 0.1880615686804319 1106 16-17 0.210863243231465 1106 18-19 0.29965131850759263 1106 20-21 0.3230068691438248 1106 22-23 0.30285003848258896 1106 24-25 0.22774497685810502 1106 26-27 0.19398656438244188 1106 28-29 0.21200141837721276 1106 30-31 0.3824869135647617 1106 32-33 0.44903316392127834 1106 34-35 0.3230563804337976 1106 36-37 0.5361592674961386 1106 38-39 0.6061813395210081 1106 40-41 0.5651585165770285 1106 42-43 0.6737838797195863 1106 44-45 0.20639685258752394 1106 46-47 0.23646780301496761 1106 48-49 0.6657577776368058 1106 50-51 0.5579302965721382 1106 52-53 0.8701737699724248 1106 54-55 0.8597728804987099 1106 56-57 0.7335264398893813 1106 58-59 1.2132752562139046 1106 60-61 1.0137401456522284 1106 62-63 1.1831623059806873 1106 64-65 0.9155786113825535 1106 66-67 1.1455534701774432 1106 68-69 0.9925795714519374 1106 70-71 1.0833163110441326 1106 72-73 1.4736863029359242 1106 74-75 1.821674193396703 1106 76-77 1.8152081111311418 1106 78-79 2.195063665502598 1106 80-81 2.140193589734782 1106 82-83 2.2987964739216267 1106 84-85 2.501542403871092 1106 86-87 2.8215269227143516 1106 88-89 3.0682059261088135 1106 90-91 2.957364829201847 1106 92-93 2.7429460739609546 1106 94-95 3.332943378847016 1106 96-97 3.985712971390324 1106 98-99 4.265626605150921 1106 100-101 4.146042055418661 1107 1 0.16626059438256036 1107 2 0.20451508067355206 1107 3 0.18193906416932037 1107 4 0.1704450143149927 1107 5 0.07089786940329645 1107 6 0.07446602311479467 1107 7 0.13014933993868993 1107 8 0.12969360652733997 1107 9 0.148207746805042 1107 10-11 0.25270862293717755 1107 12-13 0.23028907811631427 1107 14-15 0.20860871247006685 1107 16-17 0.34194164282894235 1107 18-19 0.25672926141059094 1107 20-21 0.3372965619006294 1107 22-23 0.313244229283427 1107 24-25 0.3079676692477378 1107 26-27 0.32275585207503354 1107 28-29 0.39869795339195946 1107 30-31 0.3249325011934374 1107 32-33 0.336585604460339 1107 34-35 0.44827920314568104 1107 36-37 0.5496185808905452 1107 38-39 0.55702081310978 1107 40-41 0.5996774801638622 1107 42-43 0.6778363349907224 1107 44-45 0.7165880523227983 1107 46-47 0.7270035747719845 1107 48-49 0.7866945566147976 1107 50-51 0.9135761679633418 1107 52-53 0.9208815897605547 1107 54-55 0.9680338377666118 1107 56-57 1.0326726591843212 1107 58-59 1.088686054601986 1107 60-61 1.2260166713291127 1107 62-63 1.3590615011558143 1107 64-65 1.4921755122061917 1107 66-67 1.5941220786078212 1107 68-69 1.7398436541873004 1107 70-71 1.486439285383117 1107 72-73 1.6776165386038748 1107 74-75 1.7987546180885765 1107 76-77 1.7671454079984237 1107 78-79 1.984937173596176 1107 80-81 2.261359424227795 1107 82-83 2.229650180966278 1107 84-85 2.4602550410494253 1107 86-87 2.569375614052344 1107 88-89 2.7410789537099305 1107 90-91 2.7614688184224967 1107 92-93 3.0486680422121886 1107 94-95 3.1347145998418178 1107 96-97 3.5698794699601777 1107 98-99 3.7415053737303374 1107 100-101 4.046720212730079 1108 1 0.16903773739183592 1108 2 0.23080531236281843 1108 3 0.1782405053773104 1108 4 0.1342478643392866 1108 5 0.1543680169185606 1108 6 0.13563818601389954 1108 7 0.15217499037919424 1108 8 0.10703759398776214 1108 9 0.14302327506678125 1108 10-11 0.10836097144505175 1108 12-13 0.29362265087446815 1108 14-15 0.35159018653563834 1108 16-17 0.3577171962053285 1108 18-19 0.3188596551248182 1108 20-21 0.2741539281240861 1108 22-23 0.2620457509159664 1108 24-25 0.3348492987268301 1108 26-27 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0.6646089054567881 2316 78-79 0.8397046763640041 2316 80-81 1.0100955255837327 2316 82-83 1.180226290179263 2316 84-85 1.3411132302376494 2316 86-87 1.4470807157773216 2316 88-89 1.5513812120163806 2316 90-91 1.7735760403603287 2316 92-93 1.8136363908032749 2316 94-95 2.376836643031776 2316 96-97 2.421771337318898 2316 98-99 2.5461885025026163 2316 100-101 2.8663438630437668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 14.0 4 39.0 5 70.0 6 215.0 7 522.0 8 1223.0 9 2240.0 10 3653.0 11 5466.0 12 8029.0 13 11502.0 14 14994.0 15 19575.0 16 25267.0 17 31248.0 18 29861.0 19 25593.0 20 25009.0 21 26888.0 22 29257.0 23 32753.0 24 37952.0 25 43772.0 26 51293.0 27 61183.0 28 71892.0 29 84965.0 30 100412.0 31 117514.0 32 140140.0 33 169040.0 34 208159.0 35 260965.0 36 323836.0 37 361158.0 38 203248.0 39 11587.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.02455504485862 20.251309073632857 16.296318455507635 22.427817426000882 2 15.485125760802449 21.370948687576398 39.481394205930165 23.66253134569099 3 18.823633604732862 24.942148012131305 30.660982824483817 25.57323555865202 4 13.101629221200698 18.143708874376657 37.297597084713125 31.45706481970952 5 13.390736821699164 37.84130521552708 34.491936573781665 14.276021388992099 6 31.10032949700831 37.30511517437835 17.42165737270713 14.172897955906208 7 27.730887028303837 33.33414680900392 20.8212115617985 18.113754600893746 8 26.102807317659753 35.20237240994923 20.003550427684832 18.69126984470618 9 26.173697786178447 16.796632525617593 20.343753825467743 36.68591586273621 10-11 24.544850521877446 27.208497881748716 27.53163797131239 20.715013625061456 12-13 25.612694786421308 24.46508398414667 27.858419020530683 22.06380220890134 14-15 22.264251798535664 25.94833222398869 26.541808647692477 25.24560732978317 16-17 21.471073657991475 29.072551139738458 27.39334054702565 22.06303465524442 18-19 21.49000664819552 27.387869267112215 29.974092111949474 21.148031972742793 20-21 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29.381218079296197 21.832767494267806 46-47 22.130032668270918 27.312969277970005 28.644950666955054 21.91204738680402 48-49 22.010880368299798 27.257463868887665 28.559352956203387 22.17230280660915 50-51 22.122293733731308 27.433312379774527 28.4574257667369 21.986968119757265 52-53 22.320204492038894 27.32150539708306 28.44500714218518 21.91328296869286 54-55 21.929342552899207 27.429730462708896 28.493441746023183 22.14748523836871 56-57 22.04229102564456 27.506387424085993 28.322041110173863 22.129280440095584 58-59 22.105565000183034 27.182302653098418 28.322631536063803 22.38950081065475 60-61 21.773056819832327 27.461219843505653 28.58424924789582 22.181474088766205 62-63 21.820310571890452 27.534393292132105 28.56950828151034 22.0757878544671 64-65 21.636688120119395 27.43124588915974 28.441858204105507 22.49020778661536 66-67 21.592385381895028 27.448593986816554 28.408352651774653 22.55066797951376 68-69 22.008515931207334 27.270011306684722 28.410835130036716 22.310637632071227 70-71 21.85546059320483 27.345161794406618 28.118934604034813 22.68044300835374 72-73 21.85129685129685 27.382290680163017 28.03004717898335 22.73636528955678 74-75 21.930070744830132 27.722778512748675 27.734370541054474 22.612780201366718 76-77 21.852272293399157 27.31963571509825 28.25390596247489 22.5741860290277 78-79 21.869532410248375 27.471650700894575 28.109113853398824 22.549703035458222 80-81 21.97693953013102 27.67437448288512 27.847526783696996 22.501159203286864 82-83 22.04553246445507 27.592985994582993 27.899852667783765 22.461628873178178 84-85 21.92763695387369 27.600011257952158 27.90330678726584 22.56904500090831 86-87 21.993120655019762 27.69677349391568 27.69665540708761 22.61345044397695 88-89 21.900507648579808 27.748798777581275 27.445358993000298 22.90533458083862 90-91 21.87300852543006 27.621326682718518 27.714024350161797 22.791640441689626 92-93 21.936595144873255 27.385121456892282 27.752485602419334 22.92579779581513 94-95 21.638341312611225 27.277518668282596 27.847909439689765 23.236230579416414 96-97 21.830898876183362 27.026804154551453 27.882213183895228 23.260083785369957 98-99 21.69702964607117 27.237389388629733 27.494303374205238 23.57127759109386 100-101 21.746921546194965 26.929146999601066 27.881747067185643 23.44218438701833 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1021.0 1 677.5 2 434.5 3 559.0 4 515.5 5 513.5 6 961.5 7 2280.0 8 3508.0 9 3847.5 10 3260.5 11 2499.5 12 2390.0 13 2537.5 14 2789.0 15 3004.5 16 3174.5 17 3401.0 18 3819.5 19 4296.0 20 4731.0 21 5667.5 22 7002.0 23 8465.0 24 10264.5 25 12558.0 26 15848.5 27 19899.0 28 23634.5 29 27944.5 30 32926.0 31 38750.5 32 46912.5 33 51820.0 34 53566.0 35 57409.5 36 62497.0 37 67135.0 38 70665.5 39 73442.0 40 77039.5 41 82312.0 42 86396.5 43 88930.0 44 91232.0 45 95067.5 46 97069.0 47 97980.0 48 98528.0 49 94231.5 50 89147.0 51 84619.5 52 80315.5 53 79307.5 54 79391.0 55 74527.0 56 66828.5 57 60018.0 58 54761.0 59 48009.0 60 38585.5 61 31578.0 62 26897.0 63 20880.5 64 14970.0 65 11244.0 66 8576.0 67 6615.0 68 4948.0 69 3656.0 70 2821.5 71 2076.5 72 1501.5 73 1234.5 74 971.0 75 676.5 76 490.0 77 351.5 78 242.5 79 162.5 80 98.5 81 56.0 82 29.5 83 17.0 84 11.5 85 8.0 86 5.0 87 2.0 88 1.0 89 1.5 90 1.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 7.872345199188046E-5 2 0.04388832448547336 3 1.5744690398376092E-4 4 0.0 5 0.10226176413745272 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010627666018903862 26-27 0.0117888369357841 28-29 0.0 30-31 0.0 32-33 4.920215749492529E-4 34-35 0.01505586019344714 36-37 0.003050533764685368 38-39 0.0119856455657638 40-41 0.006534046515326078 42-43 0.004979258338486439 44-45 0.0025191504637401747 46-47 1.9680862997970115E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.7712776698173104E-4 68-69 2.558512189736115E-4 70-71 0.0 72-73 3.936172599594023E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.936172599594023E-5 82-83 8.659579719106851E-4 84-85 0.004428194174543276 86-87 0.0013973412728558784 88-89 7.872345199188046E-5 90-91 8.659579719106851E-4 92-93 3.1489380796752184E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.281919309248943E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2540539.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.09631959741418 #Duplication Level Percentage of deduplicated Percentage of total 1 74.0987397290985 40.084691061509226 2 14.376193724051555 15.553983405812655 3 5.078888196313546 8.24247477201936 4 2.2680826021250073 4.907796852715566 5 1.2216914406737116 3.3044505312055232 6 0.714112069387082 2.317850084036064 7 0.45528604577919507 1.7240509608499992 8 0.31740500636420965 1.3736354132878053 9 0.22299905534548348 1.0857085351101652 >10 1.1303612817532898 11.035127572268046 >50 0.07305085050258434 2.697532103464255 >100 0.037719212115314024 3.6580857110176694 >500 0.0030636404346496746 1.1544394758326197 >1k 0.0021883145961723595 1.81460396963119 >5k 1.4588763974482396E-4 0.6291095352461632 >10k+ 7.294381987241198E-5 0.41646001599372806 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10554 0.4154236561611532 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9252 0.364174688914439 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6691 0.26336930863883606 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2859 0.11253517462239312 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.936172599594023E-5 2 0.0 0.0 0.0 0.0 3.936172599594023E-5 3 0.0 0.0 0.0 0.0 3.936172599594023E-5 4 0.0 0.0 0.0 0.0 3.936172599594023E-5 5 3.936172599594023E-5 0.0 0.0 0.0 3.936172599594023E-5 6 7.872345199188046E-5 0.0 0.0 0.0 3.936172599594023E-5 7 7.872345199188046E-5 0.0 0.0 0.0 7.872345199188046E-5 8 7.872345199188046E-5 0.0 0.0 0.0 7.872345199188046E-5 9 7.872345199188046E-5 0.0 0.0 7.872345199188046E-5 1.180851779878207E-4 10-11 7.872345199188046E-5 0.0 0.0 7.872345199188046E-5 1.3776604098579082E-4 12-13 7.872345199188046E-5 0.0 0.0 7.872345199188046E-5 1.5744690398376092E-4 14-15 1.180851779878207E-4 0.0 0.0 7.872345199188046E-5 1.5744690398376092E-4 16-17 1.180851779878207E-4 0.0 0.0 7.872345199188046E-5 1.5744690398376092E-4 18-19 1.180851779878207E-4 0.0 0.0 9.840431498985057E-5 1.5744690398376092E-4 20-21 1.180851779878207E-4 0.0 0.0 2.1648949297767127E-4 1.5744690398376092E-4 22-23 1.180851779878207E-4 0.0 0.0 4.1329812295737246E-4 1.7712776698173104E-4 24-25 1.9680862997970117E-4 0.0 0.0 9.250005609045954E-4 1.9680862997970117E-4 26-27 1.9680862997970117E-4 0.0 0.0 0.0017515968068193403 1.9680862997970117E-4 28-29 2.558512189736115E-4 0.0 0.0 0.004880854023496589 1.9680862997970117E-4 30-31 2.7553208197158164E-4 0.0 0.0 0.012930326989666367 1.9680862997970117E-4 32-33 2.7553208197158164E-4 0.0 0.0 0.02702182489621297 1.9680862997970117E-4 34-35 3.739363969614322E-4 0.0 0.0 0.04924151922092123 2.361703559756414E-4 36-37 4.723407119512828E-4 0.0 0.0 0.08569047749316189 2.361703559756414E-4 38-39 4.723407119512828E-4 0.0 0.0 0.14252880983129956 2.361703559756414E-4 40-41 4.723407119512828E-4 0.0 0.0 0.20525172020583032 2.361703559756414E-4 42-43 4.92021574949253E-4 0.0 0.0 0.27167463282397947 2.361703559756414E-4 44-45 5.904258899391034E-4 0.0 0.0 0.3570108547831779 2.7553208197158164E-4 46-47 6.494684789330137E-4 0.0 0.0 0.45769814988079305 2.7553208197158164E-4 48-49 6.691493419309839E-4 0.0 0.0 0.5640141717958276 2.7553208197158164E-4 50-51 6.691493419309839E-4 0.0 0.0 0.6778876451020827 2.7553208197158164E-4 52-53 6.691493419309839E-4 0.0 0.0 0.7873329242337945 2.7553208197158164E-4 54-55 6.691493419309839E-4 0.0 0.0 0.9100431050261382 2.7553208197158164E-4 56-57 6.691493419309839E-4 0.0 0.0 1.0533985111033526 2.7553208197158164E-4 58-59 6.691493419309839E-4 0.0 0.0 1.2160411629185774 2.7553208197158164E-4 60-61 6.691493419309839E-4 0.0 0.0 1.3931689299003085 2.7553208197158164E-4 62-63 6.888302049289541E-4 0.0 0.0 1.5704738246490213 2.7553208197158164E-4 64-65 7.085110679269242E-4 0.0 0.0 1.7559856392678876 2.7553208197158164E-4 66-67 7.478727939228644E-4 0.0 0.0 1.9733804519434655 2.7553208197158164E-4 68-69 7.478727939228644E-4 0.0 0.0 2.2155731519964856 2.7553208197158164E-4 70-71 7.872345199188047E-4 0.0 0.0 2.4683935180684102 2.952129449695517E-4 72-73 8.265962459147449E-4 0.0 0.0 2.734301658033984 3.1489380796752184E-4 74-75 8.659579719106851E-4 0.0 0.0 3.006901291418868 3.1489380796752184E-4 76-77 8.659579719106851E-4 0.0 0.0 3.3058732812210323 3.1489380796752184E-4 78-79 8.659579719106851E-4 0.0 0.0 3.642475081075315 3.1489380796752184E-4 80-81 0.0010627666018903862 0.0 0.0 3.9970455088467447 3.1489380796752184E-4 82-83 0.0010627666018903862 0.0 0.0 4.359822856488328 3.3457467096549196E-4 84-85 0.0010627666018903862 0.0 0.0 4.732775210299861 3.542555339634621E-4 86-87 0.0010824474648883564 0.0 0.0 5.117536081910178 3.542555339634621E-4 88-89 0.0011021283278863266 0.0 0.0 5.538312932806779 4.3297898595534253E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6245 0.0 45.244274 1 GTATCAA 10150 0.0 40.209705 1 TATCAAC 13190 0.0 30.725887 2 ATCAACG 13265 0.0 30.538317 3 TCAACGC 13450 0.0 30.118273 4 CAACGCA 13665 0.0 29.574818 5 AACGCAG 14190 0.0 28.584227 6 TAAGGTG 1510 0.0 25.81961 5 ACGCAGA 15790 0.0 25.477232 7 CGCAGAG 15905 0.0 25.11385 8 GCAGAGT 17165 0.0 22.882986 9 GGTAAGG 1595 0.0 22.35699 3 GTACATG 9945 0.0 22.241177 1 GAGTACT 10195 0.0 21.756077 12-13 TACATGG 9865 0.0 21.55361 2 GTAAGGT 1655 0.0 21.25918 4 AGTACTT 10550 0.0 21.249098 12-13 AGAGTAC 15615 0.0 20.956955 10-11 GTACTTT 10505 0.0 20.797577 14-15 AGGTAAG 1625 0.0 20.783304 2 >>END_MODULE