FastQCFastQC Report
Thu 26 May 2016
SRR938320_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938320_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546805
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106560.41840659178853506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86510.33968050164814345No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59430.233351198855036No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62750.045.9945451
GTATCAA104600.040.1342961
ATCAACG136600.030.7550263
TATCAAC136800.030.74482
TCAACGC139550.030.0708314
CAACGCA142600.029.3604285
AACGCAG146400.028.6895246
GTAAGGT13300.025.727374
ACGCAGA163050.025.4905957
TAAGGTG14850.025.2817215
CGCAGAG165500.025.0271388
GTACATG104400.023.9439331
TACATGG104300.023.5114782
GCAGAGT175000.023.1514459
GAGTACT100600.021.78939212-13
ACATGGG107900.021.537833
AGAGTAC156550.021.14628210-11
GTACTTT105200.020.88464514-15
AGTACTT105600.020.87014412-13
GGTAAGG16950.020.7480163
Produced by FastQC (version 0.11.5)