FastQCFastQC Report
Thu 26 May 2016
SRR938320_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938320_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2546805
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105090.4126346540076684No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90180.35409071365887845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64040.25145230985489664No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC28780.11300433287982393No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65950.046.3913421
GTATCAA106350.038.640651
TATCAAC137450.029.7190072
ATCAACG137750.029.6186393
TCAACGC140150.029.1114354
CAACGCA142500.028.6313535
TAAGGTG17900.028.1265435
AACGCAG146800.027.8250486
GTAAGGT17700.024.95594
GGTAAGG17800.024.8156973
GGACCGA5650.024.3788416
ACGCAGA167450.024.2801957
CGCAGAG168150.024.0943788
AGGTAAG18300.023.3599572
GCAGAGT179100.022.2499949
AAGGTAA18800.022.2377411
GAGTACT104800.022.18433612-13
GTACATG103100.021.7955761
TACATGG102100.021.6790772
GTACTTT109550.020.98398614-15
Produced by FastQC (version 0.11.5)