##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938320_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2546805 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77372158449508 31.0 31.0 34.0 30.0 34.0 2 32.038882050255125 31.0 31.0 34.0 30.0 34.0 3 32.085105455659146 33.0 31.0 34.0 30.0 34.0 4 35.74655813853043 37.0 35.0 37.0 33.0 37.0 5 35.64140599692556 37.0 35.0 37.0 33.0 37.0 6 35.67788974813541 37.0 35.0 37.0 33.0 37.0 7 35.60221336144699 37.0 35.0 37.0 33.0 37.0 8 35.56754678901604 37.0 35.0 37.0 33.0 37.0 9 37.106909637761824 39.0 37.0 39.0 33.0 39.0 10-11 37.03413610386347 39.0 37.0 39.0 33.0 39.0 12-13 36.918376357828734 39.0 37.0 39.0 33.0 39.0 14-15 37.894607557312 40.0 37.0 41.0 32.5 41.0 16-17 37.990884264794516 40.0 37.0 41.0 33.0 41.0 18-19 38.150719234491845 40.0 37.0 41.0 33.0 41.0 20-21 38.11871639171432 40.0 37.0 41.0 33.0 41.0 22-23 37.98967431742909 40.0 37.0 41.0 33.0 41.0 24-25 37.86488168509172 40.0 37.0 41.0 32.0 41.0 26-27 37.6340198798102 40.0 37.0 41.0 32.0 41.0 28-29 37.48198664601334 39.5 36.5 41.0 32.0 41.0 30-31 37.20347494213338 39.0 36.0 41.0 31.0 41.0 32-33 37.06034109403743 39.0 36.0 41.0 31.0 41.0 34-35 36.906613187896205 39.0 35.5 41.0 30.5 41.0 36-37 36.75363956015478 39.0 35.0 40.5 30.0 41.0 38-39 36.52191942453388 39.0 35.0 40.0 30.0 41.0 40-41 36.288402331548745 39.0 35.0 40.0 30.0 41.0 42-43 36.00133755823473 38.0 35.0 40.0 29.5 41.0 44-45 35.668931857759034 38.0 35.0 40.0 28.5 41.0 46-47 35.20847846615662 38.0 34.0 40.0 26.5 41.0 48-49 34.7554596837999 38.0 33.5 40.0 25.5 41.0 50-51 34.3433910330787 38.0 33.0 40.0 24.0 41.0 52-53 33.72420091055264 37.0 33.0 40.0 23.0 41.0 54-55 33.28038189025072 37.0 32.0 40.0 21.5 41.0 56-57 32.87723795107988 36.5 31.0 39.0 19.5 40.0 58-59 32.33417811728813 36.0 31.0 39.0 17.5 40.0 60-61 31.824408032809735 35.5 30.0 39.0 13.5 40.0 62-63 31.304808377555407 35.0 29.5 38.5 9.5 40.0 64-65 30.634457094280876 34.5 28.5 38.0 7.5 40.0 66-67 29.959426418591136 34.0 28.0 38.0 4.5 40.0 68-69 29.216242115120707 34.0 26.5 37.0 2.0 39.0 70-71 28.42352791046036 33.0 26.0 36.0 2.0 39.0 72-73 27.81634852295327 32.5 25.5 36.0 2.0 39.0 74-75 27.084054727393735 32.0 24.0 35.0 2.0 38.0 76-77 25.957965764948632 30.5 23.0 34.0 2.0 36.0 78-79 26.42649594295598 32.0 24.0 35.0 2.0 37.0 80-81 26.029812451287007 32.0 22.0 35.0 2.0 36.0 82-83 25.314736503187326 31.0 20.0 34.0 2.0 36.0 84-85 24.48927793843659 31.0 17.0 34.0 2.0 35.5 86-87 23.550338365128074 30.0 8.5 34.0 2.0 35.0 88-89 22.538718708342415 29.0 2.0 34.0 2.0 35.0 90-91 21.636027689595394 29.0 2.0 34.0 2.0 35.0 92-93 20.534019094512537 27.5 2.0 33.0 2.0 35.0 94-95 19.32568355252954 25.5 2.0 33.0 2.0 34.0 96-97 17.920302889306406 24.0 2.0 32.0 2.0 34.0 98-99 16.154431925490957 10.0 2.0 32.0 2.0 34.0 100-101 12.085204010515135 2.0 2.0 27.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.26866924494447986 1101 2 0.32928614067235884 1101 3 0.21591663621126145 1101 4 0.22483645717925071 1101 5 0.14788772554832263 1101 6 0.2482671444713418 1101 7 0.2740564701724466 1101 8 0.2774107121922782 1101 9 0.36310994891587 1101 10-11 0.3282959932727252 1101 12-13 0.26716123610642484 1101 14-15 0.49297729199039253 1101 16-17 0.3891865524161773 1101 18-19 0.4237517267578781 1101 20-21 0.4510875299123853 1101 22-23 0.3130255609466275 1101 24-25 0.3533634558526728 1101 26-27 0.5249582291034827 1101 28-29 0.5154386305673313 1101 30-31 0.6623073423245955 1101 32-33 0.5760212713886688 1101 34-35 0.3477258537039063 1101 36-37 0.35491840329685687 1101 38-39 0.3922721481359659 1101 40-41 0.453748022609922 1101 42-43 0.5344007853556789 1101 44-45 0.5061507066808915 1101 46-47 0.6371919971063775 1101 48-49 0.5925779764441117 1101 50-51 0.588930017501589 1101 52-53 0.9364516405477161 1101 54-55 0.9783189476179786 1101 56-57 0.9245567497999261 1101 58-59 0.8254491578129048 1101 60-61 0.9337133567244784 1101 62-63 1.0276318577239039 1101 64-65 1.1094379378267476 1101 66-67 1.2189440947610208 1101 68-69 1.412476530160884 1101 70-71 1.556825244730664 1101 72-73 1.53870615221782 1101 74-75 1.4452346464993475 1101 76-77 1.4794829198041057 1101 78-79 1.6515000073299042 1101 80-81 1.711922192343323 1101 82-83 1.8187659794112392 1101 84-85 1.8333513526975267 1101 86-87 2.053019518767851 1101 88-89 2.078542532731614 1101 90-91 2.235813814439986 1101 92-93 2.5274444036949717 1101 94-95 2.4018077389396595 1101 96-97 2.476409307247213 1101 98-99 2.635856512111612 1101 100-101 2.4180427462774414 1102 1 0.23944876675432525 1102 2 0.24076803566213556 1102 3 0.30695275995297777 1102 4 0.21226052868500034 1102 5 0.216472870412872 1102 6 0.19504943106349515 1102 7 0.23679516926961242 1102 8 0.2407746100846353 1102 9 0.2708115159692426 1102 10-11 0.3126446146804014 1102 12-13 0.3450418669197717 1102 14-15 0.4337842137330128 1102 16-17 0.3587752347083111 1102 18-19 0.4567887506313397 1102 20-21 0.42824547985270556 1102 22-23 0.42783662342401385 1102 24-25 0.37904578210729056 1102 26-27 0.4312101204747094 1102 28-29 0.4626637394781241 1102 30-31 0.4080507107746598 1102 32-33 0.4037082579819824 1102 34-35 0.6252029705265798 1102 36-37 0.6293224040540508 1102 38-39 0.7624768379639661 1102 40-41 0.726194494389695 1102 42-43 0.6910773096882181 1102 44-45 0.7166784330562948 1102 46-47 0.6885872334907575 1102 48-49 0.7154606869609665 1102 50-51 1.0347377247669058 1102 52-53 0.9704398229383528 1102 54-55 0.8660215577424637 1102 56-57 1.2255959254904454 1102 58-59 1.2973922351044749 1102 60-61 1.333454182527742 1102 62-63 1.365975634645764 1102 64-65 1.4928237144575789 1102 66-67 1.5179638493688827 1102 68-69 1.7883737644771536 1102 70-71 1.9922030457307258 1102 72-73 2.1322432321080527 1102 74-75 2.069906996615824 1102 76-77 1.9881249571151436 1102 78-79 2.123428999942128 1102 80-81 2.288476529265594 1102 82-83 2.334191520230732 1102 84-85 2.4977181119528495 1102 86-87 2.304521589457167 1102 88-89 2.473908737613961 1102 90-91 2.6842942304579545 1102 92-93 2.944591559137038 1102 94-95 2.9306502648200556 1102 96-97 2.983884086817959 1102 98-99 2.685585168751917 1102 100-101 2.178132286205818 1103 1 0.14971936295721733 1103 2 0.18411856449291975 1103 3 0.18697230944604826 1103 4 0.12052739625952569 1103 5 0.18758040647180252 1103 6 0.15934642978201907 1103 7 0.12893653740682964 1103 8 0.24040797841319517 1103 9 0.3112791554529295 1103 10-11 0.2890766496655601 1103 12-13 0.2682961022236654 1103 14-15 0.35730028384431733 1103 16-17 0.2949485945272343 1103 18-19 0.3221364244556568 1103 20-21 0.342850984174234 1103 22-23 0.37828155377344075 1103 24-25 0.45487418086156595 1103 26-27 0.49706944217150095 1103 28-29 0.408516804833603 1103 30-31 0.3230802804844046 1103 32-33 0.2857911727572713 1103 34-35 0.4797752923215626 1103 36-37 0.5242769228444715 1103 38-39 0.39491159326171754 1103 40-41 0.5586104617811927 1103 42-43 0.5622064345305446 1103 44-45 0.6610947113766272 1103 46-47 0.5699795274909647 1103 48-49 0.7121312622674338 1103 50-51 0.5602570199780956 1103 52-53 0.6333983170860904 1103 54-55 0.6732176720132159 1103 56-57 0.7558809603723873 1103 58-59 0.8781265747394684 1103 60-61 0.7698083405400062 1103 62-63 0.909704879831402 1103 64-65 1.180109557502714 1103 66-67 1.079344127920841 1103 68-69 0.9588423858413613 1103 70-71 1.190473834533261 1103 72-73 1.2116660374162507 1103 74-75 1.0648038928663013 1103 76-77 1.2537481868978482 1103 78-79 1.4613208395078843 1103 80-81 1.530522038169238 1103 82-83 1.6308767922745773 1103 84-85 1.5851627552910443 1103 86-87 1.72577429906978 1103 88-89 1.6899676139398139 1103 90-91 2.1052627611870385 1103 92-93 2.1928704358657143 1103 94-95 2.375959519421677 1103 96-97 2.431481091578817 1103 98-99 2.4454236431109884 1103 100-101 2.0469453374121453 1104 1 0.23542044272469909 1104 2 0.1621491822896033 1104 3 0.1558714248965387 1104 4 0.1307258678632408 1104 5 0.14150079371119517 1104 6 0.1742146005704015 1104 7 0.15002115980065867 1104 8 0.11963210564796611 1104 9 0.14327588559697801 1104 10-11 0.1930852425008993 1104 12-13 0.17749225662792156 1104 14-15 0.26197454953123867 1104 16-17 0.2365022477740908 1104 18-19 0.29395359492469453 1104 20-21 0.3218561145794183 1104 22-23 0.18977040775261855 1104 24-25 0.2538394024405619 1104 26-27 0.3451553567052059 1104 28-29 0.2794814213510577 1104 30-31 0.2254192459373101 1104 32-33 0.35797533077987964 1104 34-35 0.4139623813175817 1104 36-37 0.2352625452722492 1104 38-39 0.28103113888272446 1104 40-41 0.43134166874678925 1104 42-43 0.45270141032658984 1104 44-45 0.41111221129476405 1104 46-47 0.49793063707590335 1104 48-49 0.5381540611307329 1104 50-51 0.5382405457203987 1104 52-53 0.5096355162339563 1104 54-55 0.5369415284123491 1104 56-57 0.5792070443834021 1104 58-59 0.5733789445888959 1104 60-61 0.4799025186402055 1104 62-63 0.5729702533968251 1104 64-65 0.8166552599283996 1104 66-67 0.8820473470345362 1104 68-69 1.1189199953495397 1104 70-71 0.9623667578667927 1104 72-73 1.1387292327000118 1104 74-75 1.2408635842608753 1104 76-77 1.3174353460437551 1104 78-79 1.3026105519294013 1104 80-81 1.5993191734101089 1104 82-83 1.689397494903048 1104 84-85 1.9329147443482597 1104 86-87 1.7743987604403237 1104 88-89 1.9533220042561865 1104 90-91 2.0604716831710483 1104 92-93 2.2537647432700254 1104 94-95 2.23294996311445 1104 96-97 2.425078975605885 1104 98-99 2.3638715460800235 1104 100-101 2.232427495456987 1105 1 0.19604592214906802 1105 2 0.2220534007647217 1105 3 0.17427227764034114 1105 4 0.1822459014861053 1105 5 0.11853679704591968 1105 6 0.024720752130512835 1105 7 0.08374246323781875 1105 8 0.08495163768384373 1105 9 0.27328587025539974 1105 10-11 0.25332326378160275 1105 12-13 0.19105451174828403 1105 14-15 0.2960088711486222 1105 16-17 0.18714622666335146 1105 18-19 0.2210003494226669 1105 20-21 0.3219805639591016 1105 22-23 0.4409018636180235 1105 24-25 0.3680471149145603 1105 26-27 0.3425423503526872 1105 28-29 0.42653479879526657 1105 30-31 0.49461882829265846 1105 32-33 0.6281990726784699 1105 34-35 0.6347539540132416 1105 36-37 0.6056686837414915 1105 38-39 0.40577226407736333 1105 40-41 0.5888816647965598 1105 42-43 0.6117752772774239 1105 44-45 0.6703032357815815 1105 46-47 0.7131707913644263 1105 48-49 0.7252342059570793 1105 50-51 0.779220047027195 1105 52-53 0.6894165685790625 1105 54-55 0.9498482783629711 1105 56-57 1.0079670233002176 1105 58-59 1.0215835290341886 1105 60-61 0.9948862526613134 1105 62-63 1.2043071957143212 1105 64-65 1.374361446856824 1105 66-67 1.39267635754857 1105 68-69 1.5478495670484627 1105 70-71 1.5663206022186564 1105 72-73 1.522319110702302 1105 74-75 1.861182522040206 1105 76-77 1.7676482018512765 1105 78-79 1.8544659673003814 1105 80-81 1.9010446131025418 1105 82-83 2.099008776476161 1105 84-85 2.423901279126337 1105 86-87 2.561854435217203 1105 88-89 2.4968238806140732 1105 90-91 2.5555021050874593 1105 92-93 2.5217867215759284 1105 94-95 2.3667729504507555 1105 96-97 2.724329494647357 1105 98-99 2.869103587474733 1105 100-101 2.350840290747948 1106 1 -0.028806096595605624 1106 2 0.04970937084253535 1106 3 0.05866156578041881 1106 4 0.0877162505326865 1106 5 0.10905548359689021 1106 6 -0.013073871312180074 1106 7 0.05044739305925816 1106 8 -0.04798305226778865 1106 9 -0.14589126393402552 1106 10-11 0.049743123128180855 1106 12-13 0.055430859363490015 1106 14-15 0.08550959538495562 1106 16-17 -0.04817530919631707 1106 18-19 0.10176752994954086 1106 20-21 0.06347904938775883 1106 22-23 0.14015510530212083 1106 24-25 0.16075433710638976 1106 26-27 -0.07999057362471973 1106 28-29 0.15957607285859865 1106 30-31 0.17574408129102892 1106 32-33 0.11538810975754643 1106 34-35 0.0026064650790758037 1106 36-37 0.19435373039804205 1106 38-39 0.20366552554548178 1106 40-41 0.2619923823546486 1106 42-43 0.2209973528669522 1106 44-45 0.22822462052599946 1106 46-47 -0.11195875633830354 1106 48-49 -0.06878138535253697 1106 50-51 0.3305774345796664 1106 52-53 0.26451655706877375 1106 54-55 0.11069961503770287 1106 56-57 0.4078422050715673 1106 58-59 0.7484741362986611 1106 60-61 0.8451375700762576 1106 62-63 0.662195288949917 1106 64-65 0.7169849203193444 1106 66-67 0.9280076657552883 1106 68-69 0.8251163384473337 1106 70-71 0.7258876445081341 1106 72-73 1.1257881799418037 1106 74-75 0.8717277534767369 1106 76-77 0.9985920538768625 1106 78-79 1.1605321018727217 1106 80-81 1.2516845451348715 1106 82-83 1.4454145471183182 1106 84-85 1.5165513914940831 1106 86-87 1.5565121238605393 1106 88-89 1.7461059122593845 1106 90-91 1.6603755574667858 1106 92-93 1.564990823454501 1106 94-95 1.374515184996202 1106 96-97 1.358232159791477 1106 98-99 1.6049764204638954 1106 100-101 1.3814785931060758 1107 1 0.12288689736246639 1107 2 0.15156022428796234 1107 3 0.20726802058310767 1107 4 0.1509208187039235 1107 5 0.0982330476028963 1107 6 0.12462317181650917 1107 7 0.10599618079113071 1107 8 0.1520663619907907 1107 9 0.1590248923335622 1107 10-11 0.13135237624305063 1107 12-13 0.19209311139706386 1107 14-15 0.34122440514828867 1107 16-17 0.2785277061709337 1107 18-19 0.2655281251375641 1107 20-21 0.27942992267023214 1107 22-23 0.37322784084090443 1107 24-25 0.3412085696530198 1107 26-27 0.3844194308283235 1107 28-29 0.4139932438881786 1107 30-31 0.33406958818501664 1107 32-33 0.35563969994460365 1107 34-35 0.36435491769392314 1107 36-37 0.4048334355696923 1107 38-39 0.32699854883933455 1107 40-41 0.4795709629609419 1107 42-43 0.46016757744255443 1107 44-45 0.49010592369057093 1107 46-47 0.5008478775190497 1107 48-49 0.46985090698890986 1107 50-51 0.4020868769283794 1107 52-53 0.628477607877663 1107 54-55 0.7003707180127918 1107 56-57 0.6886372204048072 1107 58-59 0.8552832208398513 1107 60-61 1.0316645296561653 1107 62-63 1.2220536255962884 1107 64-65 1.2857383722523856 1107 66-67 1.2641878749743256 1107 68-69 1.401722500770294 1107 70-71 1.2606910863305956 1107 72-73 1.6403167539220256 1107 74-75 1.3524569471518184 1107 76-77 1.4090346777767344 1107 78-79 1.652922347207241 1107 80-81 1.7608896313189888 1107 82-83 1.8578740053394007 1107 84-85 2.048568730658353 1107 86-87 2.361771106980399 1107 88-89 2.4126088478943615 1107 90-91 2.6243531528608592 1107 92-93 2.577632066334221 1107 94-95 2.2270340637946333 1107 96-97 2.398723317218277 1107 98-99 2.57693218249295 1107 100-101 2.096680158558568 1108 1 0.05714358449118251 1108 2 0.08551828448166532 1108 3 0.07317317996943018 1108 4 0.00909597603751422 1108 5 0.06829118443901905 1108 6 0.04737163303678216 1108 7 0.12605627752769522 1108 8 0.05157917200320128 1108 9 0.0011030779196232743 1108 10-11 0.07986940210445681 1108 12-13 0.03224930514662816 1108 14-15 0.16343242382304624 1108 16-17 -2.798646149955175E-4 1108 18-19 0.013883742882704553 1108 20-21 0.024019694878219866 1108 22-23 -0.015896078506095535 1108 24-25 0.03198656760440599 1108 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66-67 -0.46171613585536875 2316 68-69 -0.27672024743150914 2316 70-71 -0.4568013649763074 2316 72-73 -0.492181272146091 2316 74-75 -0.4093491644300222 2316 76-77 -0.09128917476083487 2316 78-79 -0.14828633209628705 2316 80-81 -0.4680471828715582 2316 82-83 -0.1692583963499068 2316 84-85 0.12630930325962453 2316 86-87 0.21505952202692313 2316 88-89 0.25034331824207356 2316 90-91 0.4836721935577266 2316 92-93 0.5239451447018197 2316 94-95 0.5125015386165934 2316 96-97 0.361869509223272 2316 98-99 0.5326571124475166 2316 100-101 0.15158211716540926 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 16.0 4 39.0 5 118.0 6 233.0 7 568.0 8 1381.0 9 2534.0 10 4214.0 11 6243.0 12 8975.0 13 12641.0 14 17308.0 15 22648.0 16 28918.0 17 36117.0 18 36189.0 19 32518.0 20 33602.0 21 36151.0 22 39609.0 23 44276.0 24 49775.0 25 56322.0 26 64218.0 27 74583.0 28 87613.0 29 101910.0 30 118210.0 31 135873.0 32 160145.0 33 188023.0 34 226300.0 35 269259.0 36 303081.0 37 262856.0 38 82837.0 39 1495.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.085975196426425 20.300277349478407 16.25608837480746 22.357659079287703 2 15.536130467478356 21.33102431342982 39.496845713881804 23.63599950521002 3 18.840644014236037 24.937175472047794 30.71636453304099 25.505815980675173 4 13.1558560627924 18.197152903343603 37.30293446102077 31.344056572843225 5 13.419074917543583 37.805481388408985 34.47416365635307 14.301280037694362 6 31.01112963104753 37.351191002059444 17.481000704804647 14.156678662088382 7 27.759761740690784 33.3517485633961 20.800336107397307 18.088153588515805 8 26.138231397650856 35.22774836834587 19.913837039549993 18.72018319445328 9 26.21675478395395 16.808498524616233 20.38094938933051 36.593797302099304 10-11 24.465065052094683 27.24566662936503 27.50522713753114 20.784041181009147 12-13 25.63714143799781 24.470287281515468 27.82429357567619 22.068277704810537 14-15 22.30659591134775 25.94423208686963 26.539801830136188 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22.057629571307537 26.735302751104694 29.493691887662486 21.71337578992528 42-43 21.808625409427467 26.808946413520417 29.243472867693498 22.138955309358614 44-45 21.817802374322653 26.968031712082997 29.371410554346955 21.8427553592474 46-47 22.148655029198377 27.30084095645422 28.627425958628518 21.923078055718886 48-49 22.030504887496292 27.245175818329244 28.524190112709846 22.20012918146462 50-51 22.152540143434617 27.425008981841952 28.44736836938831 21.975082505335116 52-53 22.38276978410204 27.32871185662035 28.392986506623004 21.8955318526546 54-55 21.980343999638762 27.450374096171476 28.43429316339492 22.13498874079484 56-57 22.05887376536484 27.556546339433137 28.27334640853933 22.1112334866627 58-59 22.109977010411082 27.2254059498077 28.28039445501324 22.384222584767972 60-61 21.81230993342639 27.4421677356531 28.562296681528426 22.18322564939208 62-63 21.82556183139267 27.525625244178492 28.571720253415556 22.07709267101329 64-65 21.64657609549238 27.369463640647083 28.470629809957593 22.513330453902938 66-67 21.537761106620692 27.448144824416552 28.40507849027429 22.609015578688467 68-69 22.031448873346754 27.242856977381336 28.35491329502466 22.370780854247254 70-71 21.830271128155573 27.331246272287256 28.09439913491542 22.744083464641747 72-73 21.886691771407726 27.357937707233383 28.000313334811004 22.755057186547884 74-75 21.938880283335394 27.69452313781385 27.715235363524105 22.651361215326656 76-77 21.849179658434785 27.290369698504595 28.238027646404024 22.622422996656596 78-79 21.84370220727539 27.457166920906783 28.121705430922272 22.577425440895553 80-81 21.966886700828468 27.65488397898305 27.80004645042026 22.578182869768217 82-83 22.054484700228134 27.580857321234348 27.82923075110827 22.535427227429253 84-85 21.958838406100003 27.51425761543284 27.856495360617977 22.670408617849176 86-87 22.041016907689478 27.594106290873043 27.625068152417175 22.739808649020304 88-89 21.849375982849097 27.731922938740894 27.327003834215812 23.091697244194194 90-91 21.86935557335595 27.56251079803358 27.623666933672585 22.94446669493788 92-93 21.898466404310522 27.30893859629318 27.739480150031852 23.053114849364444 94-95 21.646177580283986 27.15781314848459 27.76072589831251 23.43528337291891 96-97 21.81009146754463 26.972932752998364 27.730234548777783 23.486741230679222 98-99 21.646710289951525 27.14724920046882 27.403825577537344 23.802214932042304 100-101 21.829088895158186 26.80104688092348 27.730309778512556 23.639554445405782 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 960.0 1 648.5 2 415.0 3 544.0 4 520.5 5 508.5 6 882.0 7 2059.0 8 3282.0 9 3691.0 10 3153.0 11 2392.0 12 2280.5 13 2449.0 14 2657.0 15 2856.0 16 3110.5 17 3469.5 18 3824.5 19 4253.5 20 4842.5 21 5820.5 22 7027.0 23 8393.5 24 10245.5 25 12693.0 26 15891.0 27 19822.5 28 23727.0 29 27800.5 30 32824.5 31 39280.5 32 47124.0 33 51782.0 34 54115.5 35 58027.0 36 62818.0 37 67235.5 38 70576.5 39 73785.5 40 77345.0 41 81969.0 42 86209.5 43 89169.0 44 92035.0 45 95643.5 46 96757.5 47 97701.0 48 98498.5 49 94276.5 50 88974.5 51 84652.0 52 80695.0 53 78941.0 54 78864.5 55 74417.5 56 66968.5 57 60672.5 58 55235.0 59 48165.0 60 38836.0 61 31908.0 62 27266.5 63 21354.0 64 15585.0 65 11707.0 66 8794.0 67 6801.0 68 5128.0 69 3641.0 70 2772.0 71 2146.5 72 1669.0 73 1376.5 74 1030.0 75 726.5 76 535.5 77 378.0 78 272.0 79 174.5 80 89.5 81 45.5 82 28.5 83 15.0 84 10.0 85 4.5 86 4.0 87 4.0 88 1.5 89 1.5 90 1.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021949069520438354 2 0.010601518373020313 3 0.0011386816030281078 4 0.0 5 1.9632441431519101E-4 6 0.0 7 0.0 8 1.1779464858911459E-4 9 3.9264882863038203E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014724331073639326 26-27 0.008225992959806503 28-29 1.374270900206337E-4 30-31 0.0 32-33 1.9632441431519101E-4 34-35 0.009718058508601954 36-37 0.001001254513007474 38-39 0.011740199976048422 40-41 0.01109232940880829 42-43 0.004260239790639645 44-45 6.08605684377092E-4 46-47 9.816220715759551E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.9632441431519101E-4 66-67 9.619896301444359E-4 68-69 0.0014331682245008943 70-71 7.85297657260764E-5 72-73 3.337515043358247E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.374270900206337E-4 82-83 0.001374270900206337 84-85 0.00720510600536751 86-87 0.004849213033585218 88-89 0.0 90-91 0.0017669197288367189 92-93 4.908110357879775E-4 94-95 7.85297657260764E-5 96-97 0.0 98-99 0.0 100-101 5.8897324294557295E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2546805.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.14830172113073 #Duplication Level Percentage of deduplicated Percentage of total 1 74.02257624046908 40.08196792444323 2 14.42441069970978 15.621146854347836 3 5.076329893089863 8.246239280610757 4 2.304073188600697 4.990466008156733 5 1.226820901920576 3.3215134177492547 6 0.7069561406447172 2.296828464434177 7 0.472380591019644 1.790502473881642 8 0.32003070401521416 1.3863295296833362 9 0.22203914489761045 1.082073835063591 >10 1.1079748867735277 10.871379078078922 >50 0.07447885899625427 2.7525320650669256 >100 0.03670591936902234 3.6520368177708087 >500 0.002829033184249504 1.0916365695090875 >1k 0.00217617937249499 1.7968089692039164 >5k 1.4507862483299934E-4 0.6057577423338683 >10k+ 7.253931241649967E-5 0.41278096966584243 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10509 0.4126346540076684 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9018 0.35409071365887845 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6404 0.25145230985489664 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2878 0.11300433287982393 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.92648828630382E-5 0.0 5 0.0 0.0 0.0 3.92648828630382E-5 0.0 6 3.92648828630382E-5 0.0 0.0 3.92648828630382E-5 0.0 7 3.92648828630382E-5 0.0 0.0 3.92648828630382E-5 3.92648828630382E-5 8 7.85297657260764E-5 0.0 0.0 3.92648828630382E-5 3.92648828630382E-5 9 7.85297657260764E-5 0.0 0.0 3.92648828630382E-5 3.92648828630382E-5 10-11 7.85297657260764E-5 0.0 0.0 3.92648828630382E-5 3.92648828630382E-5 12-13 7.85297657260764E-5 0.0 0.0 3.92648828630382E-5 3.92648828630382E-5 14-15 1.177946485891146E-4 0.0 0.0 3.92648828630382E-5 3.92648828630382E-5 16-17 1.177946485891146E-4 0.0 0.0 7.85297657260764E-5 3.92648828630382E-5 18-19 1.177946485891146E-4 3.92648828630382E-5 0.0 2.5522173860974826E-4 3.92648828630382E-5 20-21 1.177946485891146E-4 3.92648828630382E-5 0.0 2.7485418004126737E-4 3.92648828630382E-5 22-23 1.177946485891146E-4 3.92648828630382E-5 0.0 6.478705672401303E-4 3.92648828630382E-5 24-25 1.96324414315191E-4 3.92648828630382E-5 0.0 0.0011190491615965885 3.92648828630382E-5 26-27 1.96324414315191E-4 3.92648828630382E-5 0.0 0.0017669197288367189 3.92648828630382E-5 28-29 2.5522173860974826E-4 3.92648828630382E-5 0.0 0.004083547817755973 3.92648828630382E-5 30-31 2.944866214727865E-4 3.92648828630382E-5 0.0 0.010994167201650695 3.92648828630382E-5 32-33 3.141190629043056E-4 3.92648828630382E-5 0.0 0.025757763158153056 3.92648828630382E-5 34-35 3.5338394576734376E-4 3.92648828630382E-5 0.0 0.04833507080440003 7.85297657260764E-5 36-37 3.92648828630382E-4 3.92648828630382E-5 0.0 0.08441949815553212 7.85297657260764E-5 38-39 3.92648828630382E-4 3.92648828630382E-5 0.0 0.13858540406509331 7.85297657260764E-5 40-41 3.92648828630382E-4 3.92648828630382E-5 0.0 0.19689375511670504 7.85297657260764E-5 42-43 4.515461529249393E-4 3.92648828630382E-5 0.0 0.2614844874264029 7.85297657260764E-5 44-45 5.88973242945573E-4 3.92648828630382E-5 0.0 0.3440389036459407 7.85297657260764E-5 46-47 7.460327743977258E-4 3.92648828630382E-5 0.0 0.4411213265248026 7.85297657260764E-5 48-49 8.638274229868404E-4 3.92648828630382E-5 0.0 0.5449965741389702 7.85297657260764E-5 50-51 8.638274229868404E-4 3.92648828630382E-5 0.0 0.6548204515068881 7.85297657260764E-5 52-53 8.638274229868404E-4 3.92648828630382E-5 0.0 0.7680407412424587 7.85297657260764E-5 54-55 8.638274229868404E-4 3.92648828630382E-5 0.0 0.894787783124346 7.85297657260764E-5 56-57 9.030923058498786E-4 3.92648828630382E-5 0.0 1.044445884156816 7.85297657260764E-5 58-59 9.030923058498786E-4 3.92648828630382E-5 0.0 1.2058834500481976 7.85297657260764E-5 60-61 9.030923058498786E-4 3.92648828630382E-5 0.0 1.379748351365731 7.85297657260764E-5 62-63 9.030923058498786E-4 3.92648828630382E-5 0.0 1.5559887780964776 7.85297657260764E-5 64-65 9.030923058498786E-4 3.92648828630382E-5 0.0 1.7459130165049936 7.85297657260764E-5 66-67 9.030923058498786E-4 3.92648828630382E-5 0.0 1.9624784779360807 9.81622071575955E-5 68-69 9.030923058498786E-4 3.92648828630382E-5 0.0 2.2064508276055683 1.570595314521528E-4 70-71 9.030923058498786E-4 3.92648828630382E-5 0.0 2.462987939791229 1.570595314521528E-4 72-73 9.030923058498786E-4 3.92648828630382E-5 0.0 2.731147457304348 1.570595314521528E-4 74-75 9.030923058498786E-4 3.92648828630382E-5 0.0 3.0018395597621335 1.570595314521528E-4 76-77 9.030923058498786E-4 3.92648828630382E-5 0.0 3.3033349628259723 1.7669197288367188E-4 78-79 9.030923058498786E-4 3.92648828630382E-5 0.0 3.6416608260153405 1.96324414315191E-4 80-81 0.0010994167201650695 3.92648828630382E-5 0.0 3.9910201212892233 1.96324414315191E-4 82-83 0.0010994167201650695 3.92648828630382E-5 0.0 4.35206071921486 1.96324414315191E-4 84-85 0.0010994167201650695 3.92648828630382E-5 0.0 4.72556791744951 2.159568557467101E-4 86-87 0.0010994167201650695 3.92648828630382E-5 0.0 5.112896354451951 2.355892971782292E-4 88-89 0.0010994167201650695 3.92648828630382E-5 0.0 5.525982554612543 2.355892971782292E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6595 0.0 46.391342 1 GTATCAA 10635 0.0 38.64065 1 TATCAAC 13745 0.0 29.719007 2 ATCAACG 13775 0.0 29.618639 3 TCAACGC 14015 0.0 29.111435 4 CAACGCA 14250 0.0 28.631353 5 TAAGGTG 1790 0.0 28.126543 5 AACGCAG 14680 0.0 27.825048 6 GTAAGGT 1770 0.0 24.9559 4 GGTAAGG 1780 0.0 24.815697 3 GGACCGA 565 0.0 24.378841 6 ACGCAGA 16745 0.0 24.280195 7 CGCAGAG 16815 0.0 24.094378 8 AGGTAAG 1830 0.0 23.359957 2 GCAGAGT 17910 0.0 22.249994 9 AAGGTAA 1880 0.0 22.237741 1 GAGTACT 10480 0.0 22.184336 12-13 GTACATG 10310 0.0 21.795576 1 TACATGG 10210 0.0 21.679077 2 GTACTTT 10955 0.0 20.983986 14-15 >>END_MODULE