FastQCFastQC Report
Thu 26 May 2016
SRR938319_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938319_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610238
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42990.704479235970228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37190.6094343518430514No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25740.42180264093681485No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19460.31889197329566493No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11450.1876317109062366No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9970.1633788784048191No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9510.15584083587059477No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7230.11847836417922188No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35300.028.0204161
GTATCAA45700.025.2857631
ATAGCCC4150.024.063413
CGAGACA1000.001641886223.7769414
GTATAGC4400.021.61541
GAGTACT34550.021.52089512-13
GTGTAGC5550.021.4206661
TAGCCCT7150.021.2828544
TATCAAC54150.021.1643312
ATCAACG54600.020.815713
GTACATG24750.020.7507861
TCAACGC54850.020.7208354
CAACGCA55050.020.6455555
GTACTTT35850.020.54255314-15
CGCCGTA704.8457494E-420.36101594-95
TACATGG25550.020.2871742
AACGCAG56400.020.0555426
AGTACTT34050.020.02298712-13
ACATGGG25550.019.542693
AGAGTAC47000.018.90640810-11
Produced by FastQC (version 0.11.5)