##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938319_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 610238 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.967948570885458 33.0 31.0 34.0 31.0 34.0 2 32.07480360121789 33.0 31.0 34.0 31.0 34.0 3 32.11161546806328 34.0 31.0 34.0 30.0 34.0 4 35.744642254333556 37.0 35.0 37.0 35.0 37.0 5 35.75600503410145 37.0 35.0 37.0 35.0 37.0 6 35.80702938853366 37.0 35.0 37.0 35.0 37.0 7 35.76685162182624 37.0 35.0 37.0 35.0 37.0 8 35.76727604639501 37.0 35.0 37.0 35.0 37.0 9 37.42407552463138 39.0 37.0 39.0 35.0 39.0 10-11 37.33017527587597 39.0 37.0 39.0 35.0 39.0 12-13 37.25300292672695 39.0 37.0 39.0 35.0 39.0 14-15 38.42624025380262 40.0 38.0 41.0 34.5 41.0 16-17 38.30513258761336 40.0 38.0 41.0 34.0 41.0 18-19 38.46418364638059 40.0 38.0 41.0 35.0 41.0 20-21 38.481898865688464 40.0 38.0 41.0 35.0 41.0 22-23 38.407643411259215 40.0 38.0 41.0 34.0 41.0 24-25 38.33260547524081 40.0 38.0 41.0 34.0 41.0 26-27 38.17690802604885 40.0 38.0 41.0 34.0 41.0 28-29 38.01584381831351 40.0 38.0 41.0 34.0 41.0 30-31 37.80159626244186 40.0 37.5 41.0 34.0 41.0 32-33 37.536158842943244 40.0 37.0 41.0 33.0 41.0 34-35 37.31933442361833 39.5 36.5 41.0 33.0 41.0 36-37 37.11173099675864 39.0 36.0 41.0 32.5 41.0 38-39 37.003139758585995 39.0 36.0 41.0 32.5 41.0 40-41 36.920438419108606 39.0 36.0 41.0 32.0 41.0 42-43 36.645607943130386 39.0 36.0 41.0 31.0 41.0 44-45 36.263923256172184 39.0 36.0 41.0 30.5 41.0 46-47 35.81888296041872 39.0 35.0 40.0 29.5 41.0 48-49 35.438219186612436 39.0 35.0 40.0 27.5 41.0 50-51 34.73614802749091 38.5 34.5 39.5 25.5 40.5 52-53 34.76143816019324 38.5 35.0 40.0 26.0 40.5 54-55 34.932766396061865 39.0 35.0 40.0 25.5 41.0 56-57 34.70175325037117 39.0 35.0 40.0 23.5 41.0 58-59 34.449459882865376 38.0 34.5 40.0 23.0 41.0 60-61 34.10172014853221 38.0 34.0 40.0 21.5 41.0 62-63 33.676443453210055 38.0 34.0 40.0 18.5 41.0 64-65 33.30225911857341 37.5 33.0 40.0 15.5 41.0 66-67 32.892901621990106 37.0 33.0 39.5 10.5 41.0 68-69 32.44743116620073 36.5 32.0 39.0 7.5 40.5 70-71 31.943962847282535 36.0 31.5 39.0 4.0 40.0 72-73 31.39699019071248 35.0 31.0 38.5 2.0 40.0 74-75 30.84077114175125 35.0 30.5 37.5 2.0 39.5 76-77 30.200529137811806 34.5 29.5 37.0 2.0 39.0 78-79 29.5311263801992 34.0 29.0 36.0 2.0 39.0 80-81 28.913032292318732 34.0 28.5 36.0 2.0 38.0 82-83 28.27483211468312 34.0 27.5 35.0 2.0 37.0 84-85 27.640634473762695 33.5 26.0 35.0 2.0 36.5 86-87 26.997940967294728 33.0 25.5 35.0 2.0 36.0 88-89 26.31005853453898 32.0 24.5 35.0 2.0 36.0 90-91 25.519364575788465 32.0 21.5 34.0 2.0 35.0 92-93 24.7126104896778 31.0 18.5 34.0 2.0 35.0 94-95 23.86076907698308 31.0 4.5 34.0 2.0 35.0 96-97 22.77232981230274 30.5 2.0 34.0 2.0 35.0 98-99 21.365483630976765 29.5 2.0 34.0 2.0 35.0 100-101 17.339221746269487 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20496724388656062 1101 2 0.24728623550772966 1101 3 0.179667208761785 1101 4 0.21403537727810118 1101 5 0.20001805007591855 1101 6 0.2731988851559848 1101 7 0.26852872389233085 1101 8 0.21738902360021228 1101 9 0.3036271821193566 1101 10-11 0.391021326513318 1101 12-13 0.3833397630636952 1101 14-15 0.45865261232275145 1101 16-17 0.5185574181071431 1101 18-19 0.489133550008539 1101 20-21 0.6124289760048711 1101 22-23 0.649942051629715 1101 24-25 0.622194651364083 1101 26-27 0.5387262083748681 1101 28-29 0.49377188072647016 1101 30-31 0.551962171523094 1101 32-33 0.5377584156558157 1101 34-35 0.6266793584917068 1101 36-37 0.7985978268249454 1101 38-39 0.5953216007137172 1101 40-41 0.620456760755566 1101 42-43 0.7847922358652184 1101 44-45 0.9935326086727443 1101 46-47 1.1213255519674235 1101 48-49 0.9214101229064013 1101 50-51 0.9562333915317822 1101 52-53 1.0789836328720028 1101 54-55 1.0232170279001735 1101 56-57 1.2308806846504439 1101 58-59 1.3709080109034844 1101 60-61 1.353934118807686 1101 62-63 1.0520281731619363 1101 64-65 1.0742076830538068 1101 66-67 1.122929518485826 1101 68-69 1.0346222240516099 1101 70-71 0.9585193115679651 1101 72-73 1.0987796407010784 1101 74-75 0.8421887716034888 1101 76-77 0.842710953556395 1101 78-79 1.1227708489011228 1101 80-81 1.268533736635277 1101 82-83 1.3308419065620818 1101 84-85 1.3834892293367993 1101 86-87 1.5911802941323892 1101 88-89 1.3078682380901476 1101 90-91 0.7441572714726981 1101 92-93 0.9862246522534797 1101 94-95 1.137898214527521 1101 96-97 1.1666374148385152 1101 98-99 0.9726435431281111 1101 100-101 1.6112971316835498 1102 1 0.3427554360865166 1102 2 0.11201905171112969 1102 3 0.25558565306258174 1102 4 -0.06422631201299822 1102 5 -0.044199252229617514 1102 6 0.04317572204458742 1102 7 0.058242686400404864 1102 8 0.1503937639578936 1102 9 0.24033532691573356 1102 10-11 0.3704505982219999 1102 12-13 0.36831606127529426 1102 14-15 0.5187635797866577 1102 16-17 0.4826492086695282 1102 18-19 0.33800232828908605 1102 20-21 0.27098855368209485 1102 22-23 0.28065068123540016 1102 24-25 0.2837587000604884 1102 26-27 0.15279106508678808 1102 28-29 -0.021960181329120587 1102 30-31 0.21612129671163416 1102 32-33 0.17554256777820143 1102 34-35 -0.048412216599864166 1102 36-37 0.14910552990618697 1102 38-39 -0.050137352098175825 1102 40-41 0.16593456726278788 1102 42-43 0.05036404151054796 1102 44-45 0.2766679782051753 1102 46-47 0.28239805789286976 1102 48-49 0.1694681922880008 1102 50-51 0.1479808597498362 1102 52-53 0.16725394099524493 1102 54-55 0.03472451551060374 1102 56-57 0.22589253554465216 1102 58-59 0.16997340629240654 1102 60-61 -0.005980231547844994 1102 62-63 0.2407482765879223 1102 64-65 0.478829531793302 1102 66-67 0.09169837218114907 1102 68-69 0.33220895105011294 1102 70-71 0.43580464877976866 1102 72-73 0.46899080208870814 1102 74-75 0.5114222342193031 1102 76-77 0.27606987404767125 1102 78-79 0.6372774207606362 1102 80-81 0.8849795457281466 1102 82-83 0.7701814885487224 1102 84-85 0.461426365729821 1102 86-87 0.44200581664908967 1102 88-89 0.782131597279978 1102 90-91 0.4889469181005808 1102 92-93 0.5416356628115473 1102 94-95 0.6170810415044663 1102 96-97 1.1500784835737043 1102 98-99 1.2073114487518986 1102 100-101 1.04425877788049 1103 1 0.2299859253060852 1103 2 0.16784628480045427 1103 3 0.1739198864319178 1103 4 0.22833289035509807 1103 5 0.35844435351461357 1103 6 0.3043686697624963 1103 7 0.36223734618680226 1103 8 0.3208355816306039 1103 9 0.2613586678493718 1103 10-11 0.27159789411383883 1103 12-13 0.3363097810240774 1103 14-15 0.39245352738456063 1103 16-17 0.4695386842485547 1103 18-19 0.4663474620944399 1103 20-21 0.31202519514774707 1103 22-23 0.39599404496995305 1103 24-25 0.2323153923281751 1103 26-27 0.2917316227090936 1103 28-29 0.39470398523754113 1103 30-31 0.3983510859032009 1103 32-33 0.3338451498814834 1103 34-35 0.43318704737068714 1103 36-37 0.3478997316742607 1103 38-39 0.5624121498819079 1103 40-41 0.454788621424953 1103 42-43 0.49556059937286534 1103 44-45 0.3112806127105543 1103 46-47 0.4233011940183147 1103 48-49 0.19034652742141134 1103 50-51 0.42863208579887413 1103 52-53 0.4773488447914005 1103 54-55 0.41752607757308624 1103 56-57 0.3274065961070747 1103 58-59 0.4468869828354727 1103 60-61 0.6624240046215988 1103 62-63 0.7042968185296061 1103 64-65 0.7374098750070317 1103 66-67 0.8441647208350957 1103 68-69 0.6202604731109886 1103 70-71 1.0101088709486525 1103 72-73 0.8551162471065119 1103 74-75 0.926011317782649 1103 76-77 0.9440953450665113 1103 78-79 0.7105053498777956 1103 80-81 1.15639540236241 1103 82-83 0.9487236302343902 1103 84-85 1.0341755256962166 1103 86-87 0.9719482643469952 1103 88-89 1.014617146701955 1103 90-91 1.0060563524779553 1103 92-93 1.3290896609714729 1103 94-95 1.3391174229597276 1103 96-97 1.311114925031358 1103 98-99 1.6033610407355745 1103 100-101 1.3944877870408554 1104 1 0.06833216432281475 1104 2 -0.0026154272850504867 1104 3 0.037203414733532725 1104 4 0.019452863421491884 1104 5 0.02751670911400339 1104 6 0.12045438309984036 1104 7 0.012226202883923065 1104 8 0.1800387643475716 1104 9 0.10652365985939127 1104 10-11 0.23560384036992588 1104 12-13 0.2816412113025706 1104 14-15 0.31294369045613024 1104 16-17 0.4221511960438491 1104 18-19 0.1518905677092377 1104 20-21 0.2696344113704967 1104 22-23 0.22118288838995426 1104 24-25 0.24052113089206273 1104 26-27 0.22440473510471293 1104 28-29 0.20169249578100334 1104 30-31 0.20338359115478255 1104 32-33 0.21117161072107393 1104 34-35 0.12850306669839284 1104 36-37 0.12000851876129559 1104 38-39 0.02324936756514262 1104 40-41 0.005312830843173799 1104 42-43 0.02337618008120046 1104 44-45 0.07814730561535299 1104 46-47 -0.17103990485360043 1104 48-49 -0.18043146479250538 1104 50-51 -0.0491393958810562 1104 52-53 -0.005935015438389257 1104 54-55 0.007751267127787287 1104 56-57 0.12276339518146528 1104 58-59 0.1198263016240304 1104 60-61 0.22714169971238363 1104 62-63 0.19100546620681058 1104 64-65 0.1270058235866145 1104 66-67 0.25303858338838126 1104 68-69 0.16774398339365604 1104 70-71 0.2652241211513733 1104 72-73 0.12934463265530383 1104 74-75 0.23939063107068037 1104 76-77 0.30556630846431787 1104 78-79 0.4463628388886036 1104 80-81 0.29904509011283764 1104 82-83 0.09676359438615023 1104 84-85 0.3258099874272702 1104 86-87 0.5672128348082346 1104 88-89 0.5101657528407308 1104 90-91 0.6491814087872001 1104 92-93 0.7118727838465411 1104 94-95 0.9334194017258071 1104 96-97 0.956231550365068 1104 98-99 1.2464232677839284 1104 100-101 1.7258599780987112 1105 1 0.11318330139389943 1105 2 -0.0710594160058875 1105 3 0.08098339788803344 1105 4 0.0020580562292025206 1105 5 -0.009580006013983677 1105 6 0.11050272235351599 1105 7 0.15723480873590034 1105 8 0.1382683391818631 1105 9 0.1498001091214789 1105 10-11 0.13036525685654254 1105 12-13 0.23090101648072903 1105 14-15 0.17152848556361278 1105 16-17 0.18439851358147763 1105 18-19 0.28482809288593813 1105 20-21 0.3050671758631225 1105 22-23 0.397048966684892 1105 24-25 0.4153137664800113 1105 26-27 0.441233295547093 1105 28-29 0.4037812459183314 1105 30-31 0.4398431070298301 1105 32-33 0.4838237695081986 1105 34-35 0.3547774652042719 1105 36-37 0.43211255801863757 1105 38-39 0.25361612334204864 1105 40-41 0.2254418270712506 1105 42-43 0.2270419068179379 1105 44-45 0.47811800909797597 1105 46-47 0.10152070231671928 1105 48-49 0.15572089540818013 1105 50-51 0.21069324776343024 1105 52-53 0.3610290413968542 1105 54-55 0.3280034002804513 1105 56-57 0.24678379288168628 1105 58-59 0.012555272217646518 1105 60-61 0.16698084352166376 1105 62-63 0.2450346528622518 1105 64-65 0.38922109805836413 1105 66-67 0.5231066977912846 1105 68-69 0.06375050186876052 1105 70-71 0.09346413286997901 1105 72-73 -0.029657637824790584 1105 74-75 0.11529928086743624 1105 76-77 0.007968622889190868 1105 78-79 0.07850386499912432 1105 80-81 0.3102189649434699 1105 82-83 0.33098238443998085 1105 84-85 0.6235266901042387 1105 86-87 0.7058013835320018 1105 88-89 0.5078517857260714 1105 90-91 0.6919222810910028 1105 92-93 0.4066534261726851 1105 94-95 0.5753564508340929 1105 96-97 0.8991903155168579 1105 98-99 1.202590642449767 1105 100-101 1.2280709496444686 1106 1 0.09204318537426204 1106 2 -0.06628104325562845 1106 3 -0.12707104209411568 1106 4 -0.05835024363693009 1106 5 -0.016124703321736433 1106 6 -0.06419607282720818 1106 7 0.12003043568815741 1106 8 0.16392652749065917 1106 9 -0.14279620204912646 1106 10-11 -0.1145031054817025 1106 12-13 -0.12913170700580423 1106 14-15 0.2231795342026146 1106 16-17 0.013632114652430971 1106 18-19 -0.1008628189093912 1106 20-21 -0.07534335514981905 1106 22-23 0.07412765179570613 1106 24-25 -0.07625435786923163 1106 26-27 -0.10166050186478515 1106 28-29 -0.24091792112019306 1106 30-31 -0.2603744297840933 1106 32-33 -0.060538866219900456 1106 34-35 -0.3281486838363392 1106 36-37 -0.07427409972344634 1106 38-39 -0.02681114127196338 1106 40-41 0.11548347514502666 1106 42-43 -0.16446857957206618 1106 44-45 0.08154096016149026 1106 46-47 0.14202531335345725 1106 48-49 0.35607787889768616 1106 50-51 -0.003431770829458003 1106 52-53 -0.07811642937512175 1106 54-55 0.27769103972713083 1106 56-57 -0.2510780247592379 1106 58-59 0.07951756583656788 1106 60-61 0.32165509608748266 1106 62-63 0.19724162891460395 1106 64-65 0.14009496389206078 1106 66-67 -0.10710860787583698 1106 68-69 -0.12327537943422584 1106 70-71 -0.18115285359440136 1106 72-73 0.2460563291542357 1106 74-75 0.656305899936303 1106 76-77 0.48561996901443294 1106 78-79 0.45885861734182853 1106 80-81 0.6529045219864535 1106 82-83 0.39274796528186684 1106 84-85 0.48300162688841297 1106 86-87 0.6041912392511755 1106 88-89 0.5318645032628844 1106 90-91 0.6793255980625936 1106 92-93 0.7043255963348116 1106 94-95 0.4428170339071258 1106 96-97 0.23471760987949608 1106 98-99 0.3778658172229292 1106 100-101 -0.6652616399732629 1107 1 -0.1871201020007014 1107 2 -0.3383973810045724 1107 3 -0.18496506750638986 1107 4 -0.24255543422898285 1107 5 -0.3109431227143631 1107 6 -0.2419332991045806 1107 7 -0.26253917772087476 1107 8 -0.22990288586430552 1107 9 -0.15433760963676235 1107 10-11 -0.04571424362714538 1107 12-13 -0.005748134869200783 1107 14-15 -0.10501051716261856 1107 16-17 -0.04814809621537108 1107 18-19 0.09381367023181753 1107 20-21 0.06652944831057539 1107 22-23 -0.1616815759235024 1107 24-25 -0.1550549346016865 1107 26-27 -0.2666409019729201 1107 28-29 -0.34926927734259294 1107 30-31 -0.14400176149536748 1107 32-33 -0.17140554189801094 1107 34-35 -0.25966057894311945 1107 36-37 -0.07952889831766186 1107 38-39 -0.038304112345230124 1107 40-41 -0.18009528488020266 1107 42-43 -0.1123125914671732 1107 44-45 0.011523162558532363 1107 46-47 -0.1073010811675843 1107 48-49 -0.18236832277843718 1107 50-51 -0.40004459411503746 1107 52-53 -0.1008421660607084 1107 54-55 -0.05952847906172565 1107 56-57 -0.09610821309843232 1107 58-59 -0.19626980912963887 1107 60-61 -0.4622898890436318 1107 62-63 -0.20178400864509882 1107 64-65 -0.13029455579793847 1107 66-67 0.03838679628746178 1107 68-69 -0.18916480900978883 1107 70-71 -0.1569931707972465 1107 72-73 -0.008390818723569282 1107 74-75 0.050653471352003265 1107 76-77 -0.23526855941667435 1107 78-79 -0.2301519260482756 1107 80-81 -0.03871032887413506 1107 82-83 0.15894716326330283 1107 84-85 0.29088664085615434 1107 86-87 0.28634069405878293 1107 88-89 -0.032044661376207983 1107 90-91 -0.1359138810759113 1107 92-93 -0.012573115028615689 1107 94-95 0.09817546411348843 1107 96-97 0.19865606171332573 1107 98-99 0.11507745099782696 1107 100-101 0.5504678364618094 1108 1 0.06593207426315573 1108 2 0.04143500612708806 1108 3 -0.0496636346867092 1108 4 -0.0737206140072999 1108 5 -0.09711235764272175 1108 6 -0.0882701469012801 1108 7 -0.07422882357110439 1108 8 -0.04403643547230729 1108 9 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-0.6961834531402928 2316 56-57 -0.4950687764060575 2316 58-59 -0.42081779602705893 2316 60-61 -0.505453593874563 2316 62-63 -0.3751120115338509 2316 64-65 -0.6898983834010508 2316 66-67 -0.5873386170369344 2316 68-69 -0.6768514820385967 2316 70-71 -0.7249189400577762 2316 72-73 -0.5779195902280456 2316 74-75 -0.7118778020224319 2316 76-77 -0.888589340412782 2316 78-79 -1.0274978804987676 2316 80-81 -1.012671536993608 2316 82-83 -0.7763098587376795 2316 84-85 -0.6008442920576229 2316 86-87 -0.5632633914690608 2316 88-89 -1.0763019670943983 2316 90-91 -1.4205931516647539 2316 92-93 -1.0041931403052402 2316 94-95 -1.6456885063799227 2316 96-97 -1.4537993817882615 2316 98-99 -1.8664602962875172 2316 100-101 -1.8077277220742083 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3543.0 3 608.0 4 445.0 5 367.0 6 410.0 7 420.0 8 467.0 9 570.0 10 742.0 11 1068.0 12 1820.0 13 2475.0 14 3330.0 15 4585.0 16 6359.0 17 8452.0 18 7143.0 19 4657.0 20 4258.0 21 4162.0 22 4357.0 23 4744.0 24 5280.0 25 6219.0 26 7170.0 27 8780.0 28 10567.0 29 13008.0 30 16391.0 31 21053.0 32 27257.0 33 35725.0 34 48387.0 35 66362.0 36 89220.0 37 113299.0 38 71880.0 39 4658.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.263972809048795 20.985803085202793 15.446171192209807 22.304052913538612 2 15.081286411588726 22.04475673122343 40.75982128798614 22.1141355692017 3 18.458321031394547 25.715316733917266 31.534133779483646 24.29222845520454 4 11.919505405729039 18.602892569439547 38.724736766995115 30.752865257836298 5 12.262516204197503 38.375806107546644 35.598201539193646 13.763476149062207 6 29.197171271878037 37.88376761394946 18.58829279172899 14.330768322443518 7 25.170296601279762 34.379261512640305 22.70861743417602 17.741824451903913 8 23.905154355287614 35.317643742029816 21.511885209626183 19.265316693056388 9 24.73284119293783 17.21289442801791 22.74652365748602 35.30774072155824 10-11 23.024311873965146 27.789308021442977 29.179576714372367 20.006803390219513 12-13 24.344974854805457 26.035666419381677 28.906768094498386 20.712590631314477 14-15 21.339563990581894 27.7403022283997 28.57426182364738 22.345871957371028 16-17 19.642494197558385 30.32857554975676 29.429852742555173 20.59907751012969 18-19 18.887732354194668 29.03050519621021 32.32468937481559 19.75707307477953 20-21 20.689016347887105 27.803704034631977 32.21795324748568 19.289326369995237 22-23 19.7455637983655 28.451271443416914 31.756171999724632 20.046992758492955 24-25 19.492709076155112 28.260997860673275 32.3530954664306 19.89319759674101 26-27 19.54397607743357 28.403293287516725 32.341040841486766 19.711689793562943 28-29 19.01138409178096 29.045582270719734 32.221783223547234 19.72125041395207 30-31 19.781144988166123 28.626671683870264 32.03189367463435 19.560289653329267 32-33 19.27673118364182 28.699766965111976 32.11602727565576 19.907474575590438 34-35 19.902955997373468 28.850547481307267 31.87909422318481 19.367402298134458 36-37 19.6405136877102 28.626605888867125 31.766537181865353 19.966343241557325 38-39 19.178695183238865 28.918597294116488 32.08450394867541 19.81820357396923 40-41 19.93816986403763 28.66593941400702 31.6695038262724 19.726386895682953 42-43 19.436747385390312 28.780580955167466 31.43276758924832 20.3499040701939 44-45 19.907915362106984 28.815985591597503 31.225935124496168 20.050163921799346 46-47 19.843583805744984 28.954941843790305 30.76780619076417 20.43366815970054 48-49 19.747584128381053 28.74020683941798 31.064835414264724 20.447373617936243 50-51 19.775971418738425 29.15518117307724 30.584899785312768 20.483947622871565 52-53 19.932430217770378 29.42096154602546 30.193105686153686 20.453502550050477 54-55 20.003834883675196 29.061371249516544 30.20954197722669 20.72525188958157 56-57 20.074520643135934 29.18572225777718 30.002220705332068 20.73753639375482 58-59 20.178549770707612 29.09276262091527 30.137854059245612 20.590833549131506 60-61 20.043188756172018 29.00850253027106 30.301706652461636 20.64660206109529 62-63 19.704678518312143 28.892421529624336 30.45842842100519 20.94447153105833 64-65 19.760238614202134 28.913780953473506 30.203788983759157 21.1221914485652 66-67 19.737473921928853 28.781422744175146 30.099379370586394 21.38172396330961 68-69 19.932020226768593 28.587313774671962 30.25280183027509 21.227864168284356 70-71 19.958612494109452 28.27823877722458 30.18509640010654 21.578052328559426 72-73 19.77818354070405 27.958085491043256 30.712064763259733 21.551666204992962 74-75 19.875920963128692 28.332718674960454 30.04032158926069 21.751038772650162 76-77 19.5959860848757 28.1718165465415 30.217618448602956 22.01457891997984 78-79 19.739419926239172 28.067297536689907 30.12362026202219 22.06966227504873 80-81 19.83997377962595 28.13257676629043 29.756847000020485 22.270602454063134 82-83 19.933644924139877 27.999662406875096 30.009980301605044 22.056712367379983 84-85 20.053243703428148 27.887966988576117 29.85727934344237 22.201509964553363 86-87 19.801777366236596 27.937673362860505 29.980374402320653 22.280174868582243 88-89 20.25733618770674 27.74285395814824 29.616528264525666 22.383281589619347 90-91 20.35078085621673 27.575964954697636 29.49493293774869 22.57832125133694 92-93 20.46763209008433 27.444824166728132 29.711930109245284 22.375613633942255 94-95 20.31220927611546 27.431547972770826 29.662995946771147 22.59324680434257 96-97 20.21915297677739 27.32297811338941 29.70917027919096 22.748698630642238 98-99 20.704870003482963 27.06468069413428 29.234444467208913 22.996004835173842 100-101 21.07955705350562 26.574740047272332 29.631946454206258 22.713756445015786 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1604.0 1 925.5 2 278.5 3 308.5 4 233.0 5 223.5 6 411.5 7 755.5 8 1058.5 9 1077.0 10 842.5 11 628.0 12 633.5 13 700.5 14 776.5 15 861.0 16 923.5 17 1047.0 18 1249.0 19 1450.5 20 1710.0 21 1995.0 22 2362.5 23 2814.5 24 3275.0 25 3976.5 26 4987.0 27 6299.5 28 7620.0 29 9083.0 30 10776.0 31 12366.5 32 14066.0 33 16050.5 34 18044.5 35 19649.0 36 21178.0 37 22364.0 38 22603.0 39 22562.5 40 22728.0 41 22801.5 42 22911.0 43 22906.0 44 22806.0 45 22782.5 46 22733.5 47 23203.5 48 24162.0 49 23463.0 50 21108.0 51 18935.5 52 16958.0 53 14908.0 54 13095.5 55 11552.0 56 9857.5 57 8106.0 58 6468.5 59 5171.5 60 3926.0 61 2841.5 62 2102.0 63 1475.0 64 1021.5 65 752.0 66 560.5 67 414.0 68 321.0 69 251.0 70 197.5 71 164.5 72 138.0 73 113.0 74 87.0 75 63.0 76 52.5 77 35.5 78 26.5 79 21.0 80 13.5 81 11.5 82 7.5 83 4.5 84 3.0 85 1.5 86 1.5 87 1.5 88 2.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005735467145605485 2 0.0888178055119478 3 0.09471714314742773 4 0.09946938735378656 5 0.13568476561603834 6 0.08095202199797455 7 0.020647681724179745 8 0.024908314460915248 9 0.01868123584568644 10-11 0.040639881488861725 12-13 0.045965672409781104 14-15 0.02236832186786139 16-17 0.024580573481166365 18-19 0.029168947197650754 20-21 0.04612954289965554 22-23 0.02359735054191971 24-25 0.038181824140745085 26-27 0.04424503226609945 28-29 0.0432618093268528 30-31 0.055142419842749875 32-33 0.03990246428442673 34-35 0.05055404612626549 36-37 0.042032780652794484 38-39 0.02621927837991079 40-41 0.029414752932462415 42-43 0.025645731665350238 44-45 0.0063090138601660335 46-47 0.011634804781085414 48-49 0.008930941698157112 50-51 0.007865783513973237 52-53 0.008849006453219892 54-55 0.008193524493722121 56-57 0.011716740026022633 58-59 0.016387048987444242 60-61 0.020565746479242527 62-63 0.009094812188031555 64-65 0.007865783513973237 66-67 0.008357394983596564 68-69 0.009258682677905998 70-71 0.024662508726103583 72-73 0.022777998092547498 74-75 0.023433480052045267 76-77 0.018107689131125888 78-79 0.003687086022174955 80-81 0.004178697491798282 82-83 0.0058993376354799275 84-85 0.0040967622468610606 86-87 0.010733517086775978 88-89 0.02949668817739964 90-91 0.027939918523592438 92-93 0.023433480052045267 94-95 0.037772147916058985 96-97 0.03400312664894681 98-99 0.020565746479242527 100-101 0.0602224050288576 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 610238.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.0321723013611 #Duplication Level Percentage of deduplicated Percentage of total 1 82.80067380028775 61.299137494517 2 11.821918502495324 17.50404615018765 3 3.187757098305006 7.079897482698093 4 1.0986024873354139 3.2532771453247693 5 0.4575800779409679 1.693782358589799 6 0.20769327959854533 0.9225590796644565 7 0.13265105381571515 0.6874311970429505 8 0.06625077876008907 0.39237512546130154 9 0.04683137500654086 0.31203255812345 >10 0.15792723265880565 2.008464983720671 >50 0.01017261791343831 0.516692124043923 >100 0.00950912820283311 1.3830779105074786 >500 0.0013268550979454474 0.7070920330475478 >1k 0.0011057125816212063 2.2401343570709877 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4299 0.704479235970228 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3719 0.6094343518430514 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2574 0.42180264093681485 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1946 0.31889197329566493 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1145 0.1876317109062366 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 997 0.1633788784048191 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 951 0.15584083587059477 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 723 0.11847836417922188 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.193524493722122E-5 0.0 22-23 0.0 0.0 0.0 4.096762246861061E-4 0.0 24-25 0.0 0.0 0.0 0.0016387048987444243 0.0 26-27 0.0 0.0 0.0 0.004670308961421609 0.0 28-29 0.0 0.0 0.0 0.013355444924767059 0.0 30-31 0.0 0.0 0.0 0.03506828483313068 0.0 32-33 0.0 0.0 0.0 0.07890364087454403 0.0 34-35 0.0 0.0 0.0 0.1599375981174558 0.0 36-37 0.0 0.0 0.0 0.2843972351770948 0.0 38-39 0.0 0.0 0.0 0.44081161776225014 0.0 40-41 0.0 0.0 0.0 0.5990285757360243 0.0 42-43 0.0 0.0 0.0 0.7596216558129779 0.0 44-45 0.0 0.0 0.0 0.9689662066275782 0.0 46-47 0.0 0.0 0.0 1.2246041708317084 0.0 48-49 0.0 0.0 0.0 1.475244085094668 0.0 50-51 0.0 0.0 0.0 1.7248188411734438 0.0 52-53 0.0 0.0 0.0 1.9693136120661119 0.0 54-55 0.0 0.0 0.0 2.255267616897014 0.0 56-57 0.0 0.0 0.0 2.60775304061694 0.0 58-59 0.0 0.0 0.0 2.9911280516781975 0.0 60-61 0.0 0.0 0.0 3.3740114512698325 0.0 62-63 0.0 0.0 0.0 3.7450961755905072 0.0 64-65 0.0 0.0 0.0 4.173125895142551 0.0 66-67 0.0 0.0 0.0 4.664327688541192 0.0 68-69 0.0 0.0 0.0 5.194039047060327 0.0 70-71 0.0 0.0 0.0 5.758409014187907 0.0 72-73 0.0 0.0 0.0 6.291561652994405 0.0 74-75 0.0 0.0 0.0 6.866255460984075 0.0 76-77 0.0 0.0 0.0 7.493879437203189 0.0 78-79 0.0 0.0 0.0 8.20827283781082 0.0 80-81 0.0 0.0 0.0 8.940364251324894 0.0 82-83 0.0 0.0 0.0 9.664835687059803 0.0 84-85 0.0 0.0 0.0 10.40323611443404 0.0 86-87 0.0 0.0 0.0 11.19382929283329 0.0 88-89 0.0 0.0 0.0 12.046611322139887 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3530 0.0 28.020416 1 GTATCAA 4570 0.0 25.285763 1 ATAGCCC 415 0.0 24.06341 3 CGAGACA 100 0.0016418862 23.776941 4 GTATAGC 440 0.0 21.6154 1 GAGTACT 3455 0.0 21.520895 12-13 GTGTAGC 555 0.0 21.420666 1 TAGCCCT 715 0.0 21.282854 4 TATCAAC 5415 0.0 21.164331 2 ATCAACG 5460 0.0 20.81571 3 GTACATG 2475 0.0 20.750786 1 TCAACGC 5485 0.0 20.720835 4 CAACGCA 5505 0.0 20.645555 5 GTACTTT 3585 0.0 20.542553 14-15 CGCCGTA 70 4.8457494E-4 20.361015 94-95 TACATGG 2555 0.0 20.287174 2 AACGCAG 5640 0.0 20.055542 6 AGTACTT 3405 0.0 20.022987 12-13 ACATGGG 2555 0.0 19.54269 3 AGAGTAC 4700 0.0 18.906408 10-11 >>END_MODULE