FastQCFastQC Report
Thu 26 May 2016
SRR938319_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938319_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences610238
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41550.6808818854283083No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38440.6299181630773567No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26490.434092927677398No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19580.32085841917415825No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14890.2440031594230448No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10270.16829499310105236No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT9960.16321500791494464RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT8210.13453767218691723No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6920.11339837899311417No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36150.027.998161
ACCGGAT350.00840647227.13311810-11
GTATCAA47800.023.659591
AGTACTT36000.022.09276412-13
ATGCCGC652.935753E-421.92958594-95
GCGTGCG1553.9133727E-521.4439169
GAGTACT36000.021.3673312-13
AGAGTAC48150.020.21600310-11
GTACTTT38200.020.074614-15
TATCAAC57700.019.517792
TAGCCCT6850.019.4170484
ATCAACG58300.019.3105873
TCAACGC58750.019.2435344
CAACGCA58700.019.1790035
TACATGG22300.018.9645822
AACGCAG59700.018.9295546
GTACATG22750.018.7983281
GTAGCCC3801.0913936E-1118.7509563
TACTTTT43050.018.75052814-15
GCTTAGG1801.2285431E-418.4792231
Produced by FastQC (version 0.11.5)