##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938319_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 610238 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29923079192053 31.0 31.0 34.0 30.0 34.0 2 31.5525991498399 31.0 31.0 34.0 30.0 34.0 3 31.576902782193176 31.0 31.0 34.0 30.0 34.0 4 35.35814059432549 37.0 35.0 37.0 33.0 37.0 5 35.252925907596705 37.0 35.0 37.0 33.0 37.0 6 35.264908117816326 37.0 35.0 37.0 32.0 37.0 7 35.19816694470026 37.0 35.0 37.0 32.0 37.0 8 35.18963093088271 37.0 35.0 37.0 32.0 37.0 9 36.66682999092158 39.0 35.0 39.0 32.0 39.0 10-11 36.54567070552801 38.0 35.0 39.0 32.0 39.0 12-13 36.42790681668464 38.0 35.0 39.0 32.0 39.0 14-15 37.25386652420859 39.0 36.0 40.0 32.0 41.0 16-17 37.132726903273806 39.0 36.0 40.0 31.5 41.0 18-19 37.4364379143875 39.0 36.0 40.5 32.0 41.0 20-21 37.44106561702156 39.0 36.0 41.0 32.0 41.0 22-23 37.284895401466315 39.0 36.0 40.0 31.5 41.0 24-25 37.13235655596669 39.0 36.0 40.0 31.0 41.0 26-27 36.86418167993472 39.0 36.0 40.0 31.0 41.0 28-29 36.71477194143924 39.0 35.5 40.0 30.5 41.0 30-31 36.391308800828526 38.0 35.0 40.0 30.0 41.0 32-33 36.07298054201803 38.0 35.0 40.0 30.0 41.0 34-35 35.83123633729791 38.0 34.5 40.0 29.5 41.0 36-37 35.72465660938847 38.0 34.5 40.0 29.0 41.0 38-39 35.49501997581272 38.0 34.0 40.0 28.0 41.0 40-41 35.24871279730203 38.0 34.0 40.0 27.0 41.0 42-43 34.93505402810051 38.0 34.0 40.0 26.5 41.0 44-45 34.46371006066485 38.0 33.0 40.0 24.5 41.0 46-47 33.86284941285203 38.0 33.0 40.0 23.5 41.0 48-49 33.344649136894134 37.0 32.5 40.0 20.0 41.0 50-51 32.85738924812942 37.0 31.5 40.0 16.5 41.0 52-53 32.277218069015696 36.5 31.0 39.0 13.5 40.0 54-55 31.85567680151023 36.0 30.0 39.0 9.0 40.0 56-57 31.419710834133568 36.0 30.0 39.0 8.0 40.0 58-59 30.830755377410124 35.0 28.5 38.5 6.5 40.0 60-61 30.323839223384976 35.0 28.0 38.0 2.0 40.0 62-63 29.757437262182954 34.0 27.5 38.0 2.0 40.0 64-65 29.12602623894284 34.0 26.0 38.0 2.0 40.0 66-67 28.497718922780948 33.5 26.0 37.5 2.0 39.0 68-69 27.92199191135262 33.0 25.0 37.0 2.0 39.0 70-71 27.25683831554246 32.0 23.0 36.0 2.0 39.0 72-73 26.660459525627708 31.5 22.0 36.0 2.0 38.5 74-75 25.986222588563805 31.0 20.5 35.0 2.0 38.0 76-77 25.01660171932918 30.5 20.5 34.0 2.0 36.0 78-79 25.64732366715937 31.0 21.0 35.0 2.0 37.0 80-81 25.135867481212248 31.0 19.0 34.5 2.0 36.0 82-83 24.533230477289187 31.0 16.5 34.0 2.0 36.0 84-85 23.791945929293163 30.0 9.0 34.0 2.0 35.5 86-87 22.970848259203787 29.5 4.5 34.0 2.0 35.0 88-89 21.987975183453013 29.0 2.0 34.0 2.0 35.0 90-91 21.123383007941165 28.5 2.0 33.0 2.0 35.0 92-93 20.031810539494426 26.0 2.0 33.0 2.0 34.0 94-95 18.93730396992649 25.5 2.0 32.0 2.0 34.0 96-97 17.581201924495033 22.0 2.0 32.0 2.0 34.0 98-99 15.988874832442423 10.0 2.0 31.5 2.0 34.0 100-101 12.798516316584678 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.24629443798258066 1101 2 0.31877790129110295 1101 3 0.31504047479834796 1101 4 0.3282259779405834 1101 5 0.15131587628651744 1101 6 0.4186196599471188 1101 7 0.2427823329853922 1101 8 0.288647452042035 1101 9 0.457680930707582 1101 10-11 0.3108668329121542 1101 12-13 0.4171425071300803 1101 14-15 0.6168018354538916 1101 16-17 0.44569042441010964 1101 18-19 0.4279488115718877 1101 20-21 0.4626197931614584 1101 22-23 0.14244404937100796 1101 24-25 0.41375663464329904 1101 26-27 0.4292168670125349 1101 28-29 0.3585554714415835 1101 30-31 0.840382109049564 1101 32-33 0.6985789432876714 1101 34-35 0.5377066188300432 1101 36-37 0.6204762214046156 1101 38-39 0.5086930008735564 1101 40-41 0.6767171835982282 1101 42-43 0.8991198952223769 1101 44-45 0.8906545778639909 1101 46-47 0.9105005031819928 1101 48-49 1.2603468858256548 1101 50-51 1.127694489221497 1101 52-53 1.1566896999193474 1101 54-55 1.1432492157643885 1101 56-57 1.0776520512622874 1101 58-59 1.5024387280073874 1101 60-61 1.6707297911159067 1101 62-63 1.3076037530975348 1101 64-65 1.5329241969412735 1101 66-67 1.1609086142242546 1101 68-69 1.3031033455697916 1101 70-71 1.20713684706298 1101 72-73 1.2109477099630972 1101 74-75 1.2480734781436027 1101 76-77 1.2545688194769973 1101 78-79 1.3430491473586024 1101 80-81 1.7495493965363984 1101 82-83 1.358114905815217 1101 84-85 1.6063773797052718 1101 86-87 1.7781326430169706 1101 88-89 1.3843455382701215 1101 90-91 1.5188702457847292 1101 92-93 1.9547224858889543 1101 94-95 1.7992145180680872 1101 96-97 1.8008022555832675 1101 98-99 1.5983301891638728 1101 100-101 1.9650810782060386 1102 1 0.4122392774568304 1102 2 0.47558947207325986 1102 3 0.4163494599308635 1102 4 0.24882768015993406 1102 5 0.17349320875796792 1102 6 0.25565370433410806 1102 7 0.21989394686171693 1102 8 0.29241819110688994 1102 9 0.1276362205158108 1102 10-11 0.49146234034590464 1102 12-13 0.5851706799376686 1102 14-15 0.561898527977057 1102 16-17 0.6664173690341144 1102 18-19 0.674660188430316 1102 20-21 0.6762846821940016 1102 22-23 0.502979224979704 1102 24-25 0.693816158452826 1102 26-27 0.7267780068530882 1102 28-29 0.623079550303899 1102 30-31 0.6011785430077552 1102 32-33 0.7100608437401661 1102 34-35 0.8875679095001558 1102 36-37 0.871895635324023 1102 38-39 0.8288529879453037 1102 40-41 1.0992042553500028 1102 42-43 1.0017365187948784 1102 44-45 0.7633032527185435 1102 46-47 0.7392119877758248 1102 48-49 0.42213179209585405 1102 50-51 0.6360183957071683 1102 52-53 0.7704300037331819 1102 54-55 1.2224387762039477 1102 56-57 1.1856487114648324 1102 58-59 1.238869454143991 1102 60-61 0.947326968448376 1102 62-63 1.0027071790833126 1102 64-65 1.226193427710502 1102 66-67 1.3677026780036101 1102 68-69 1.333891430034349 1102 70-71 1.1850497429905822 1102 72-73 1.5139818082177818 1102 74-75 1.6965840362129825 1102 76-77 1.6138461842690681 1102 78-79 1.8882320282032445 1102 80-81 1.8104804997469124 1102 82-83 1.7242752160931722 1102 84-85 1.7460293943572864 1102 86-87 1.7421409386429154 1102 88-89 1.830807777011774 1102 90-91 2.2129973196912154 1102 92-93 2.8734967263543716 1102 94-95 3.0830892217957278 1102 96-97 3.032716174997187 1102 98-99 3.3721141258154557 1102 100-101 3.0288981434936897 1103 1 0.16749948786524627 1103 2 0.3298661431401051 1103 3 0.22315673222175292 1103 4 0.1909378645673101 1103 5 0.26410183310294144 1103 6 0.07036129263764224 1103 7 0.15154621295235415 1103 8 0.2189952541894158 1103 9 0.28722510407115465 1103 10-11 0.05914879780419824 1103 12-13 0.35582025592070465 1103 14-15 0.4510078423496324 1103 16-17 0.28251576296987224 1103 18-19 0.4494186787699377 1103 20-21 0.5327976927144249 1103 22-23 0.5693256288358626 1103 24-25 0.5627871672931732 1103 26-27 0.6858576200376163 1103 28-29 0.7729617955937087 1103 30-31 0.8646653324793405 1103 32-33 0.8933756763334628 1103 34-35 0.7636692035519204 1103 36-37 0.6486242044667918 1103 38-39 0.6853497478156996 1103 40-41 1.0521848745747704 1103 42-43 1.0733631338594805 1103 44-45 1.3238681753154466 1103 46-47 0.9335697620867975 1103 48-49 0.9836980047443618 1103 50-51 1.2811954527894613 1103 52-53 1.060170343346762 1103 54-55 1.368923385588328 1103 56-57 1.5240147461062143 1103 58-59 1.359209267736535 1103 60-61 1.5416182400992433 1103 62-63 1.7760931145207302 1103 64-65 1.5070271610598382 1103 66-67 1.3278523339898491 1103 68-69 1.4990655369986285 1103 70-71 1.3031082153294768 1103 72-73 1.0956263239984132 1103 74-75 1.5180743958273695 1103 76-77 1.2754788995776813 1103 78-79 1.4871897365115778 1103 80-81 1.5098897261159685 1103 82-83 1.494796036926008 1103 84-85 1.6041487191302757 1103 86-87 2.2092421226902736 1103 88-89 2.0031659213375477 1103 90-91 2.3938083021305125 1103 92-93 2.719421813357851 1103 94-95 2.401524459205227 1103 96-97 2.303699514330763 1103 98-99 2.854244661036864 1103 100-101 2.7544621660545943 1104 1 0.07826297007626692 1104 2 0.09288839655303605 1104 3 0.12049371992618418 1104 4 -0.11320080553876721 1104 5 0.12966381120516246 1104 6 -0.08711965705627733 1104 7 0.19463286043041705 1104 8 0.14480693711266213 1104 9 0.10118173528472596 1104 10-11 0.26315540791662784 1104 12-13 0.352606479953252 1104 14-15 0.41803552041724146 1104 16-17 0.4906394678572781 1104 18-19 0.37867293100688926 1104 20-21 0.23335106439336073 1104 22-23 0.3336115802131303 1104 24-25 0.6938478200536267 1104 26-27 0.3279027243341943 1104 28-29 0.377865323041803 1104 30-31 0.43444611763171537 1104 32-33 0.5976134507076694 1104 34-35 0.6666892757225824 1104 36-37 0.8890172548392599 1104 38-39 0.49075093042835505 1104 40-41 0.5440692308000834 1104 42-43 0.4779148174465817 1104 44-45 0.5955893177173763 1104 46-47 0.6087661924364127 1104 48-49 0.47673344044750365 1104 50-51 0.44622658291004313 1104 52-53 0.23843473836025453 1104 54-55 0.3596625896245733 1104 56-57 0.5850184020579228 1104 58-59 0.5713552307787033 1104 60-61 0.6948671056161722 1104 62-63 0.7709150350571257 1104 64-65 1.1631673580354978 1104 66-67 1.473191825381054 1104 68-69 1.355552836004268 1104 70-71 1.1057958851463 1104 72-73 1.0339943754377963 1104 74-75 1.57599230524891 1104 76-77 1.0852947268612283 1104 78-79 1.4024627000137038 1104 80-81 1.3144966520343395 1104 82-83 1.552250432366261 1104 84-85 1.7887482003739628 1104 86-87 1.7608699932762235 1104 88-89 2.006283675227035 1104 90-91 2.2050940970273523 1104 92-93 2.5793872454097873 1104 94-95 3.124478240578366 1104 96-97 3.1138721643978577 1104 98-99 2.880611612371455 1104 100-101 2.1226262239238984 1105 1 0.19846108777502636 1105 2 0.22865944426974139 1105 3 0.11873592888359497 1105 4 0.19795109690382873 1105 5 0.14746602309222823 1105 6 0.23812203921207953 1105 7 0.2725661742327361 1105 8 0.2081194577117742 1105 9 0.2808838263236595 1105 10-11 0.20766368418279058 1105 12-13 0.05198987981409431 1105 14-15 0.3707010457404252 1105 16-17 0.057298200050489356 1105 18-19 0.28019772318472036 1105 20-21 0.44714697752919363 1105 22-23 0.46728838469550027 1105 24-25 0.346050094195796 1105 26-27 0.12576819838846376 1105 28-29 0.4424435447431989 1105 30-31 0.6462530718925237 1105 32-33 0.5450353571785342 1105 34-35 0.9311750302910085 1105 36-37 0.8905278565164849 1105 38-39 0.8307644800676428 1105 40-41 0.8269614379264638 1105 42-43 0.8212356692428244 1105 44-45 0.9407824000772109 1105 46-47 0.8727461243220134 1105 48-49 0.9858771581755548 1105 50-51 1.110136020050703 1105 52-53 1.4039737942799775 1105 54-55 1.4881072192067286 1105 56-57 1.189742007726803 1105 58-59 1.6060304056422972 1105 60-61 1.0993581701783732 1105 62-63 0.8530894775077762 1105 64-65 1.08503263067621 1105 66-67 1.2765206421679594 1105 68-69 1.0868648622246475 1105 70-71 1.0215592425271929 1105 72-73 1.2653430223049007 1105 74-75 1.226395590120923 1105 76-77 1.2743053285384534 1105 78-79 1.337538870958884 1105 80-81 1.2368494370345218 1105 82-83 1.329754826843871 1105 84-85 1.5150477755744625 1105 86-87 1.7917045778149863 1105 88-89 2.0078331863414007 1105 90-91 2.074221060809535 1105 92-93 2.2197953825174856 1105 94-95 2.427408807832183 1105 96-97 2.634816885531631 1105 98-99 2.792323810708874 1105 100-101 2.42515625285421 1106 1 -0.10129348607593158 1106 2 0.10741520806566385 1106 3 0.1836808939289618 1106 4 0.021560348725934375 1106 5 0.12138019831161984 1106 6 -0.07210033175326203 1106 7 -0.1483396713719074 1106 8 0.11281230316767221 1106 9 -0.0827745107282567 1106 10-11 0.07423180853204059 1106 12-13 -0.12164912063213507 1106 14-15 -0.07417103517583712 1106 16-17 0.23173669477010606 1106 18-19 0.08028696905709864 1106 20-21 0.16195063660995146 1106 22-23 0.23443102934817972 1106 24-25 0.15870813376146486 1106 26-27 -0.07097246798071666 1106 28-29 0.44118852466287706 1106 30-31 0.5495700434825963 1106 32-33 0.30134764336030173 1106 34-35 0.14863747265304994 1106 36-37 0.25680779106951945 1106 38-39 0.35425659668224796 1106 40-41 0.5537389182180021 1106 42-43 0.8478606878357127 1106 44-45 0.39186450627979497 1106 46-47 -0.19597319740935149 1106 48-49 0.3227431406238708 1106 50-51 0.8725746635134399 1106 52-53 0.7517630996496081 1106 54-55 1.3141707325192407 1106 56-57 0.856529528178978 1106 58-59 1.0152227304972676 1106 60-61 0.9539152875366952 1106 62-63 0.9644237032998362 1106 64-65 0.9961958018795443 1106 66-67 0.7761487843663843 1106 68-69 0.4695158364920893 1106 70-71 0.4842247192488962 1106 72-73 1.2237693200886248 1106 74-75 1.3433076829366328 1106 76-77 1.2007704482766677 1106 78-79 1.611077469798687 1106 80-81 0.7122789327953463 1106 82-83 1.1631997175176743 1106 84-85 0.9467653867599459 1106 86-87 1.289743027911669 1106 88-89 1.8266826583033229 1106 90-91 1.8977373481812414 1106 92-93 1.6803877519953971 1106 94-95 1.7521894117292547 1106 96-97 2.0505817970294764 1106 98-99 2.2574714382561023 1106 100-101 1.8896531292486767 1107 1 0.1295797228994644 1107 2 0.20775539009684252 1107 3 0.12377506352434864 1107 4 0.11121070467799399 1107 5 0.20001856904244875 1107 6 0.09875215089970624 1107 7 0.1258222642863842 1107 8 0.25455556711288096 1107 9 0.16096253532023042 1107 10-11 0.259054283074704 1107 12-13 0.10480735409076658 1107 14-15 0.3659400308780505 1107 16-17 0.396006317190583 1107 18-19 0.1823314405358758 1107 20-21 0.32141265337124736 1107 22-23 0.14627769961942505 1107 24-25 0.551191462589081 1107 26-27 0.47902978488041725 1107 28-29 0.4431349966823319 1107 30-31 0.3579923648307002 1107 32-33 0.463885941999564 1107 34-35 0.6835264065991637 1107 36-37 0.44633649252035923 1107 38-39 0.5231749980519922 1107 40-41 0.7740428291849284 1107 42-43 0.4751304454278582 1107 44-45 0.5637454200402701 1107 46-47 0.6317910632016179 1107 48-49 0.46260413909192266 1107 50-51 0.522155631342585 1107 52-53 0.38899050345243325 1107 54-55 0.5765130322398839 1107 56-57 0.7327032688296065 1107 58-59 0.18793832930775878 1107 60-61 0.6990558660714363 1107 62-63 0.61512997526523 1107 64-65 0.3489825911018585 1107 66-67 0.6569093502850976 1107 68-69 0.8112613532984305 1107 70-71 1.0566001360282016 1107 72-73 1.1931473051296315 1107 74-75 0.944466779989888 1107 76-77 1.1229059353452442 1107 78-79 1.474736566851945 1107 80-81 1.2860150776993713 1107 82-83 1.3915688713430328 1107 84-85 1.4572637196427074 1107 86-87 1.8256637722569913 1107 88-89 1.5199770171390448 1107 90-91 1.8462639769998432 1107 92-93 1.9630215719467365 1107 94-95 1.923976831752686 1107 96-97 1.9610733713288369 1107 98-99 1.6765182527104603 1107 100-101 1.190450642089985 1108 1 0.040928736146288 1108 2 0.16562508460887315 1108 3 0.15337225195365534 1108 4 0.20686899070124554 1108 5 0.17533081559557218 1108 6 0.2596280633084689 1108 7 0.23258625455402182 1108 8 0.00768884637754752 1108 9 0.1482131435927343 1108 10-11 0.10355279618635649 1108 12-13 0.2510360645530483 1108 14-15 0.30820550803403535 1108 16-17 0.2230947194614643 1108 18-19 0.3151943764645111 1108 20-21 0.5289876736469878 1108 22-23 0.4231038688543549 1108 24-25 0.37756615845282226 1108 26-27 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22 10027.0 23 11259.0 24 12668.0 25 14392.0 26 16500.0 27 19033.0 28 22065.0 29 25989.0 30 29861.0 31 34358.0 32 39819.0 33 46417.0 34 54546.0 35 61978.0 36 64862.0 37 48122.0 38 11490.0 39 163.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.42310843673595 23.07287672310385 16.55572598142358 20.948288858736618 2 15.977743751495934 23.967927132753868 38.6736744777749 21.380654637975297 3 18.83035799147218 26.981112287337073 32.38048105821007 21.808048662980674 4 12.881859209029919 20.582461269209716 37.639904430730304 28.895775091030057 5 12.069513842441411 39.229485514944066 35.41658470052852 13.284415942086007 6 28.64243131368417 39.41478570656039 17.79273004958721 14.150052930168231 7 24.90175964132027 35.12367305870824 22.258200898665766 17.71636640130572 8 24.09273103281015 36.32943212320439 20.937240879787886 18.640595964197576 9 24.489953100265797 17.386167364208717 22.14152510987516 35.98235442565032 10-11 23.10082295760015 28.53804581163415 28.616703646773882 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19.71788508882063 28.63231962970369 32.094259030398 19.555536251077683 38-39 19.36679383558501 28.882748946267856 32.29525148636867 19.455205731778463 40-41 20.02923485469835 28.682686296407667 32.015115599761074 19.27296324913291 42-43 19.64996661081318 28.72994088270358 31.685723766955764 19.93436873952747 44-45 20.070960340871846 28.781055391674858 31.393477548344805 19.754506719108488 46-47 20.317728492817558 28.848416519456343 30.841737158289064 19.99211782943704 48-49 20.099289129814924 28.775002539992595 31.016423100495217 20.109285229697267 50-51 20.077281449666277 29.148101475328435 30.544935820017464 20.229681254987817 52-53 20.216046853850465 29.586079529626147 30.018206011425054 20.179667605098338 54-55 20.332036563023365 29.20559981908579 29.94351347188128 20.518850146009562 56-57 20.510931800379524 29.34953247749239 29.646547740389817 20.492987981738274 58-59 20.451539305013988 29.557393466456215 29.538380595070095 20.452686633459706 60-61 20.37328058888499 29.626883281604883 29.61696911696748 20.382867012542647 62-63 19.975892972006513 29.52512760254705 29.758741255876192 20.740238169570244 64-65 20.02451838018076 29.549728798197833 29.39329301021299 21.032459811408422 66-67 20.010078538857684 29.32895777256097 29.30044288213435 21.360520806446985 68-69 20.082784007657235 29.09650540366013 29.4251993798165 21.395511208866132 70-71 20.094487879299354 28.737359419373128 29.51320552506649 21.65494717626103 72-73 19.90923198691656 28.446324952436512 29.859300566828956 21.785142493817975 74-75 19.95055018451485 28.70597028257857 29.252180127140274 22.091299405766303 76-77 19.51033777807652 28.405835354602633 29.821967091905115 22.261859775415736 78-79 19.467975596083047 28.510112020666618 29.587946222212025 22.433966161038313 80-81 19.480718513406973 28.33895815883808 29.530661718787137 22.649661608967815 82-83 19.444207661918266 28.258036490008685 29.740668491713524 22.557087356359524 84-85 19.410959689909777 28.1860869137664 29.493481057781345 22.90947233854248 86-87 19.10818996174937 28.1587909260455 29.604169876070575 23.128849236134556 88-89 19.32739415456897 28.08373340111595 29.13081089058983 23.458061553725244 90-91 19.31441937006635 27.681463245128633 29.35283955311951 23.651277831685505 92-93 19.19055580141286 27.602727377030373 29.408385373129438 23.79833144842733 94-95 18.943930633469517 27.631547675147623 29.42250560365846 24.002016087724403 96-97 18.62814606202825 27.599612149268438 29.451001059786552 24.321240728916763 98-99 18.820134684826847 27.43070043487706 29.10012009328748 24.649044787008613 100-101 18.669007032570093 27.226830295610505 29.513439895371878 24.590722776447528 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1616.0 1 943.0 2 295.5 3 312.0 4 238.5 5 213.0 6 385.0 7 678.0 8 927.5 9 975.5 10 784.5 11 593.5 12 594.0 13 622.0 14 649.5 15 737.5 16 836.5 17 947.0 18 1130.5 19 1345.5 20 1629.0 21 1915.5 22 2271.0 23 2819.0 24 3437.0 25 4132.5 26 5102.0 27 6369.0 28 7762.0 29 9277.0 30 11046.5 31 12754.5 32 14406.5 33 16285.5 34 18145.0 35 19871.5 36 21425.5 37 22623.5 38 22909.5 39 22791.0 40 22975.0 41 23250.0 42 23131.5 43 23194.5 44 23387.0 45 23146.5 46 23080.5 47 23638.5 48 24343.5 49 23521.5 50 21315.5 51 18964.5 52 16634.5 53 14415.5 54 12520.5 55 10864.5 56 9264.5 57 7704.0 58 6179.0 59 4862.0 60 3697.5 61 2662.0 62 1942.0 63 1358.0 64 910.5 65 645.5 66 444.5 67 322.0 68 244.0 69 161.0 70 117.5 71 92.0 72 68.5 73 58.0 74 43.0 75 26.5 76 17.5 77 16.0 78 12.5 79 7.0 80 4.5 81 2.5 82 1.5 83 1.0 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.2774097974888486E-4 2 0.04129536344835949 3 0.0 4 0.0 5 0.03769021267112176 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00606320812535437 26-27 0.0020483811234305303 28-29 8.193524493722122E-5 30-31 0.0010651581841838757 32-33 5.735467145605485E-4 34-35 0.011143193311462084 36-37 0.0216309046634264 38-39 0.004260632736735503 40-41 0.0012290286740583183 42-43 0.0017206401436816454 44-45 0.006227078615228812 46-47 0.0 48-49 0.0 50-51 1.6387048987444243E-4 52-53 0.0 54-55 6.554819594977697E-4 56-57 0.0 58-59 0.020483811234305305 60-61 0.0 62-63 0.006964495819663803 64-65 0.013683185904515944 66-67 0.004998049941170494 68-69 0.015731567027946473 70-71 1.6387048987444243E-4 72-73 1.6387048987444243E-4 74-75 0.02040187598936808 76-77 0.04367148555153891 78-79 0.028185724258404096 80-81 0.023761221031794153 82-83 0.03408506189388402 84-85 0.015239955558323148 86-87 0.008521265473471006 88-89 0.07300430323906411 90-91 0.023269609562170827 92-93 0.02097542270392863 94-95 0.02384315627673137 96-97 0.03433086762869569 98-99 0.04842372975789774 100-101 0.012618027720332067 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 610238.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.21075491846445 #Duplication Level Percentage of deduplicated Percentage of total 1 83.32514914544605 61.83622221777193 2 11.63132043194479 17.263381399063654 3 2.964711664003936 6.60040472103927 4 1.0477194527793452 3.110082061340626 5 0.4342781542244415 1.611405483479656 6 0.20826937076007454 0.9273496338299217 7 0.10802410177003857 0.5611585099220515 8 0.062414951562540226 0.3705488538924401 9 0.040881221241108684 0.2730443661262295 >10 0.15296072285077825 1.9668133819862699 >50 0.010590917126425951 0.5405106904239056 >100 0.011032150234297338 1.764471285912212 >500 0.0013238580280790085 0.6985184210838378 >1k 0.0013238580280790085 2.476088974127941 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4155 0.6808818854283083 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3844 0.6299181630773567 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2649 0.434092927677398 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1958 0.32085841917415825 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1489 0.2440031594230448 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1027 0.16829499310105236 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 996 0.16321500791494464 RNA PCR Primer, Index 39 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 821 0.13453767218691723 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 692 0.11339837899311417 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.6387048987444243E-4 0.0 10-11 0.0 0.0 0.0 1.6387048987444243E-4 0.0 12-13 0.0 0.0 0.0 1.6387048987444243E-4 0.0 14-15 0.0 0.0 0.0 1.6387048987444243E-4 0.0 16-17 0.0 0.0 0.0 1.6387048987444243E-4 0.0 18-19 0.0 0.0 0.0 1.6387048987444243E-4 0.0 20-21 0.0 0.0 0.0 2.4580573481166365E-4 0.0 22-23 0.0 0.0 0.0 5.735467145605485E-4 0.0 24-25 0.0 0.0 0.0 0.0018025753886188665 0.0 26-27 0.0 0.0 0.0 0.004670308961421609 0.0 28-29 0.0 0.0 0.0 0.013355444924767059 0.0 30-31 0.0 0.0 0.0 0.034740543853381795 0.0 32-33 0.0 0.0 0.0 0.07792041793529739 0.0 34-35 0.0 0.0 0.0 0.1575614760142764 0.0 36-37 0.0 0.0 0.0 0.2802185376852966 0.0 38-39 0.0 0.0 0.0 0.43499421537170746 0.0 40-41 0.0 0.0 0.0 0.5916544036916744 0.0 42-43 0.0 0.0 0.0 0.7488062034812646 0.0 44-45 0.0 0.0 0.0 0.9527430281300083 0.0 46-47 0.0 0.0 0.0 1.201580367004349 0.0 48-49 0.0 0.0 0.0 1.4461570731419546 0.0 50-51 0.0 0.0 0.0 1.6895866858504387 0.0 52-53 0.0 0.0 0.0 1.9258059970044474 0.0 54-55 0.0 0.0 0.0 2.2013542257283225 0.0 56-57 0.0 0.0 0.0 2.539255175849423 0.0 58-59 0.0 0.0 0.0 2.90673474939286 0.0 60-61 0.0 0.0 0.0 3.272493682792615 0.0 62-63 0.0 0.0 0.0 3.6252249122473525 0.0 64-65 0.0 0.0 0.0 4.032934691054965 0.0 66-67 0.0 0.0 0.0 4.499719781462314 0.0 68-69 0.0 0.0 0.0 5.00231057390723 0.0 70-71 0.0 0.0 0.0 5.539887715940338 0.0 72-73 0.0 0.0 0.0 6.0518191263080965 0.0 74-75 0.0 0.0 0.0 6.603898806695092 0.0 76-77 0.0 0.0 0.0 7.2057951160039195 0.0 78-79 0.0 0.0 0.0 7.890118281719592 0.0 80-81 0.0 0.0 0.0 8.586813669420785 0.0 82-83 0.0 0.0 0.0 9.266712331909844 0.0 84-85 0.0 0.0 0.0 9.961932885202167 0.0 86-87 0.0 0.0 0.0 10.69484365116561 0.0 88-89 0.0 0.0 0.0 11.476915564091389 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3615 0.0 27.99816 1 ACCGGAT 35 0.008406472 27.133118 10-11 GTATCAA 4780 0.0 23.65959 1 AGTACTT 3600 0.0 22.092764 12-13 ATGCCGC 65 2.935753E-4 21.929585 94-95 GCGTGCG 155 3.9133727E-5 21.443916 9 GAGTACT 3600 0.0 21.36733 12-13 AGAGTAC 4815 0.0 20.216003 10-11 GTACTTT 3820 0.0 20.0746 14-15 TATCAAC 5770 0.0 19.51779 2 TAGCCCT 685 0.0 19.417048 4 ATCAACG 5830 0.0 19.310587 3 TCAACGC 5875 0.0 19.243534 4 CAACGCA 5870 0.0 19.179003 5 TACATGG 2230 0.0 18.964582 2 AACGCAG 5970 0.0 18.929554 6 GTACATG 2275 0.0 18.798328 1 GTAGCCC 380 1.0913936E-11 18.750956 3 TACTTTT 4305 0.0 18.750528 14-15 GCTTAGG 180 1.2285431E-4 18.479223 1 >>END_MODULE