FastQCFastQC Report
Thu 26 May 2016
SRR938318_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938318_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643551
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45450.7062377340723579No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38000.5904737930637975No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26020.40431916040842136No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20250.31466037656689216No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12620.1960994544332928No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9990.1552324524396668No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9940.15445551323826706No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7050.10954842739736245No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCCG301.2978668E-463.362153
TCGCCGG404.4758574E-429.7079494-95
TAGGACG1205.3578533E-627.720944
GCTCGGA700.00837027427.1552092
GTATCAA44450.026.3265361
GTACATG24700.024.8440931
GGTATCA33050.024.1806641
TACATGG24650.024.0981832
GTCTAGC1652.396384E-623.0640961
GTGTAGC5350.022.2288321
GTAGCCC4300.022.1030753
ACATGGG25600.022.0901263
TAGCCCT7750.022.0745564
AGTACTT35100.021.92623312-13
CATGGGG10200.021.8972154
TATCAAC53450.021.6936862
ATCAACG54250.021.1985823
TCAACGC54400.021.1401314
CAACGCA54900.020.9475965
AACGCAG55700.020.5566236
Produced by FastQC (version 0.11.5)