##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938318_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 643551 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8492458251172 33.0 31.0 34.0 31.0 34.0 2 32.00268510188004 33.0 31.0 34.0 30.0 34.0 3 32.068857013663255 33.0 31.0 34.0 30.0 34.0 4 35.69291633452516 37.0 35.0 37.0 35.0 37.0 5 35.7280712795101 37.0 35.0 37.0 35.0 37.0 6 35.79284780848759 37.0 35.0 37.0 35.0 37.0 7 35.755458386359436 37.0 35.0 37.0 35.0 37.0 8 35.74723681573022 37.0 35.0 37.0 35.0 37.0 9 37.39513573904787 39.0 37.0 39.0 35.0 39.0 10-11 37.30471089315377 39.0 37.0 39.0 35.0 39.0 12-13 37.22798581619794 39.0 37.0 39.0 35.0 39.0 14-15 38.411508955778174 40.0 38.0 41.0 34.0 41.0 16-17 38.30830423696024 40.0 38.0 41.0 34.0 41.0 18-19 38.349615648177064 40.0 38.0 41.0 34.0 41.0 20-21 38.433470696184145 40.0 38.0 41.0 34.0 41.0 22-23 38.36289043137218 40.0 38.0 41.0 34.0 41.0 24-25 38.299729158994396 40.0 38.0 41.0 34.0 41.0 26-27 38.153419076343596 40.0 38.0 41.0 34.0 41.0 28-29 38.014441745875615 40.0 38.0 41.0 34.0 41.0 30-31 37.82292856354819 40.0 37.5 41.0 34.0 41.0 32-33 37.547349782690105 40.0 37.0 41.0 33.0 41.0 34-35 37.329575278416165 40.0 36.0 41.0 33.0 41.0 36-37 37.105835434953875 39.0 36.0 41.0 32.5 41.0 38-39 36.90219345475339 39.0 36.0 41.0 32.0 41.0 40-41 36.834288191611854 39.0 36.0 41.0 31.5 41.0 42-43 36.65339732204596 39.0 36.0 41.0 31.0 41.0 44-45 36.32040661890045 39.0 36.0 41.0 30.5 41.0 46-47 35.90085090381338 39.0 35.0 41.0 29.5 41.0 48-49 35.52188404648582 39.0 35.0 40.5 27.5 41.0 50-51 34.781607829060945 38.5 34.5 39.5 26.5 40.5 52-53 34.835566256598156 38.5 35.0 40.0 26.0 40.5 54-55 35.0288625143928 39.0 35.0 40.0 26.0 41.0 56-57 34.82041594217086 39.0 35.0 40.0 24.5 41.0 58-59 34.58511757420935 38.5 35.0 40.0 23.0 41.0 60-61 34.25644976078042 38.0 34.0 40.0 22.0 41.0 62-63 33.87035060158402 38.0 34.0 40.0 20.0 41.0 64-65 33.51569106411147 38.0 34.0 40.0 18.0 41.0 66-67 33.08682062493882 37.0 33.0 39.5 14.5 41.0 68-69 32.62217912799452 36.5 32.5 39.0 9.5 40.5 70-71 32.10354035655294 36.0 32.0 39.0 7.5 40.0 72-73 31.64392332542409 35.5 31.0 38.5 2.0 40.0 74-75 31.143837085172734 35.0 31.0 38.0 2.0 39.5 76-77 30.523817071218907 35.0 30.5 37.0 2.0 39.0 78-79 29.855605072480657 34.0 29.0 36.5 2.0 39.0 80-81 29.276779151924245 34.0 29.0 36.0 2.0 38.5 82-83 28.70099261752371 34.0 28.5 35.5 2.0 37.0 84-85 28.1220563716007 34.0 27.0 35.0 2.0 37.0 86-87 27.561693634226344 33.5 26.0 35.0 2.0 36.0 88-89 26.905343166275866 33.0 25.5 35.0 2.0 36.0 90-91 26.201669331568127 32.0 24.5 35.0 2.0 35.0 92-93 25.521517331182764 32.0 21.5 34.0 2.0 35.0 94-95 24.72238563843425 31.5 18.0 34.0 2.0 35.0 96-97 23.66610571656326 31.0 3.5 34.0 2.0 35.0 98-99 22.363398549609897 31.0 2.0 34.0 2.0 35.0 100-101 18.62415566132288 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6427483203988587 1101 2 0.6344362153478471 1101 3 0.6091932059854557 1101 4 0.5024378707890236 1101 5 0.4282348221706016 1101 6 0.33913721488035975 1101 7 0.34036091607232777 1101 8 0.4753525459068584 1101 9 0.501664949297151 1101 10-11 0.5447826147088719 1101 12-13 0.5707606532276088 1101 14-15 0.726327346524485 1101 16-17 0.5675893679155166 1101 18-19 0.6008963743537024 1101 20-21 0.7019467247326219 1101 22-23 0.7383877150258016 1101 24-25 0.7926495316132858 1101 26-27 0.8348028737180684 1101 28-29 0.8535554977937281 1101 30-31 0.8415732344374476 1101 32-33 0.7522388067719206 1101 34-35 0.7340423713804682 1101 36-37 0.7046958618087942 1101 38-39 0.8731232427240201 1101 40-41 1.0829630450027423 1101 42-43 1.1307441929282973 1101 44-45 1.1905500138076022 1101 46-47 1.2039299506436194 1101 48-49 1.0324061923485814 1101 50-51 0.8626592444547043 1101 52-53 0.7530520501198552 1101 54-55 0.8843966333262401 1101 56-57 0.9446976320155756 1101 58-59 1.0619601370406642 1101 60-61 1.140702686749968 1101 62-63 1.1929952566539015 1101 64-65 1.2317394438442406 1101 66-67 0.973816107280733 1101 68-69 1.0247585011066676 1101 70-71 1.2652877901393609 1101 72-73 0.6913199711438942 1101 74-75 0.6320222741441377 1101 76-77 0.9168709144258003 1101 78-79 0.8377191644189921 1101 80-81 1.1361614021423883 1101 82-83 1.257893055296016 1101 84-85 1.3690672930594303 1101 86-87 1.0606072163368516 1101 88-89 1.295602858388044 1101 90-91 1.0001871023247553 1101 92-93 1.1250758250729724 1101 94-95 1.5436986365532732 1101 96-97 1.4551599047546055 1101 98-99 1.2172632371448664 1101 100-101 0.7180797144627178 1102 1 0.4132202543432584 1102 2 0.41166016302857855 1102 3 0.4187253121390313 1102 4 0.3945243288856304 1102 5 0.4025852501684781 1102 6 0.27745249402588 1102 7 0.3295410286900591 1102 8 0.2667269367358358 1102 9 0.314583162563828 1102 10-11 0.3390090622002262 1102 12-13 0.4453951506141749 1102 14-15 0.49836734349441514 1102 16-17 0.540557324888475 1102 18-19 0.546420703318681 1102 20-21 0.5209604358151125 1102 22-23 0.4374256666961216 1102 24-25 0.6277302073196793 1102 26-27 0.6137388129145975 1102 28-29 0.5542890280818327 1102 30-31 0.3562728784038143 1102 32-33 0.25320590522405695 1102 34-35 0.5256566430265579 1102 36-37 0.6504676467745796 1102 38-39 0.5836817862687056 1102 40-41 0.5684048044654091 1102 42-43 0.498730204089064 1102 44-45 0.45703613859612346 1102 46-47 0.16296335720372923 1102 48-49 0.040815908784203714 1102 50-51 -0.013088804935492249 1102 52-53 -0.03457514983974619 1102 54-55 -0.18834785572762058 1102 56-57 0.12287857790352064 1102 58-59 0.22201240580817938 1102 60-61 0.0640582406221526 1102 62-63 -0.151511472634283 1102 64-65 0.019770653601078436 1102 66-67 0.02772742713023746 1102 68-69 -0.11998179634554873 1102 70-71 -0.06340448064820947 1102 72-73 0.09757328528676013 1102 74-75 0.17217958545893808 1102 76-77 0.2847496104841838 1102 78-79 0.4309406375397131 1102 80-81 0.49112819758717663 1102 82-83 0.42498140718855026 1102 84-85 0.3317633009376202 1102 86-87 0.050484245854818255 1102 88-89 -0.028034239811585593 1102 90-91 0.19841198427814533 1102 92-93 0.13324439816087263 1102 94-95 0.23183084875941873 1102 96-97 0.40977697921865897 1102 98-99 0.7547389334305024 1102 100-101 0.6937797876894933 1103 1 0.48255967508801945 1103 2 0.45017730765215447 1103 3 0.4288860578469311 1103 4 0.3830058050829024 1103 5 0.24944642865328603 1103 6 0.38569903418852647 1103 7 0.3454967047256332 1103 8 0.2989022351616697 1103 9 0.3484206096556619 1103 10-11 0.28016210352804904 1103 12-13 0.3455128564209957 1103 14-15 0.3692226723573029 1103 16-17 0.24547600087649357 1103 18-19 0.3756985365806784 1103 20-21 0.17038260730890897 1103 22-23 0.45986110684450665 1103 24-25 0.5665624230423205 1103 26-27 0.47626437528678167 1103 28-29 0.3759421407465027 1103 30-31 0.4059304615112467 1103 32-33 0.3570628510556091 1103 34-35 0.30058530617393586 1103 36-37 0.3760478531758693 1103 38-39 0.18263194558767282 1103 40-41 0.35048617603529664 1103 42-43 -0.14513958237595403 1103 44-45 -0.1490465170371067 1103 46-47 -0.05982876720774044 1103 48-49 -0.07049692709347966 1103 50-51 -0.07846903035995467 1103 52-53 -0.09353624747611633 1103 54-55 -0.1558253846190496 1103 56-57 -0.016626582128822065 1103 58-59 -0.070293914636963 1103 60-61 -0.1131591465467281 1103 62-63 -0.42413119965193147 1103 64-65 -0.3531534439877433 1103 66-67 -0.16234723877550294 1103 68-69 -0.29673787186749934 1103 70-71 -0.3297917446421188 1103 72-73 -0.2827662781316995 1103 74-75 -0.41830645629690366 1103 76-77 -0.33386098541632947 1103 78-79 -0.3186800881938652 1103 80-81 -0.06304025250080159 1103 82-83 -0.06452256389624722 1103 84-85 -0.004761167602826077 1103 86-87 -0.2863457822043429 1103 88-89 0.15784581916021523 1103 90-91 0.10648493809942039 1103 92-93 0.14967565321389742 1103 94-95 0.21711643395940072 1103 96-97 -0.6081606723721507 1103 98-99 -0.25037829679730095 1103 100-101 0.1843823463046057 1104 1 0.4941710067372398 1104 2 0.5286688269592688 1104 3 0.5306724165621972 1104 4 0.4878325824195997 1104 5 0.4228314881985753 1104 6 0.4508109071585764 1104 7 0.32662265203707364 1104 8 0.3577438086008584 1104 9 0.44886268832761544 1104 10-11 0.40541923091887355 1104 12-13 0.41728297551157567 1104 14-15 0.46673259657239186 1104 16-17 0.4906086244780852 1104 18-19 0.6316350216041329 1104 20-21 0.5468667285647797 1104 22-23 0.642042629760418 1104 24-25 0.5844846532841075 1104 26-27 0.5576113619342067 1104 28-29 0.5985377740918665 1104 30-31 0.6163959974188629 1104 32-33 0.669490674945898 1104 34-35 0.6554617045860667 1104 36-37 0.9061904009925428 1104 38-39 0.7227663735920018 1104 40-41 0.7714713799328052 1104 42-43 0.7987074042714681 1104 44-45 0.8690516424721011 1104 46-47 1.1029599372310912 1104 48-49 1.0049631956059102 1104 50-51 1.0455295261178605 1104 52-53 1.159964293844702 1104 54-55 1.2124216381164317 1104 56-57 1.2408391342190725 1104 58-59 1.2338968107329435 1104 60-61 1.299751355077241 1104 62-63 1.519458659604659 1104 64-65 1.522369832807641 1104 66-67 1.602516528817425 1104 68-69 1.408364076887466 1104 70-71 1.4168565772633315 1104 72-73 1.4587394959570794 1104 74-75 1.5067612088059477 1104 76-77 1.5367046948435075 1104 78-79 1.3651118641699043 1104 80-81 1.074085333930089 1104 82-83 1.0620653105173226 1104 84-85 1.3162195252878242 1104 86-87 1.4172991113358933 1104 88-89 1.3307436899649758 1104 90-91 1.4422617334801515 1104 92-93 1.631135415032734 1104 94-95 1.820111742512271 1104 96-97 1.6348111789450606 1104 98-99 1.5485886826325093 1104 100-101 0.8847567598737136 1105 1 0.3517325419896409 1105 2 0.35103011189877265 1105 3 0.4145689539005346 1105 4 0.33016675012923713 1105 5 0.21924367009260948 1105 6 0.2864909238461024 1105 7 0.14639737043506784 1105 8 0.22958360835054492 1105 9 0.20064616767680832 1105 10-11 0.14050996934189897 1105 12-13 0.3083342896542547 1105 14-15 0.4406896261927429 1105 16-17 0.3348670725381595 1105 18-19 0.3356504575657553 1105 20-21 0.3608581762076639 1105 22-23 0.20970011828774915 1105 24-25 0.3898611655911637 1105 26-27 0.40593739447488275 1105 28-29 0.37000731935678033 1105 30-31 0.4163751380112686 1105 32-33 0.32653593986037066 1105 34-35 0.2940881125960786 1105 36-37 0.4821186446304253 1105 38-39 0.3452684595327682 1105 40-41 0.32068915214807703 1105 42-43 0.020880954542640495 1105 44-45 -0.12491998873358057 1105 46-47 -0.17069984583106645 1105 48-49 0.1945022026981107 1105 50-51 0.13520411935940757 1105 52-53 0.18932391003160376 1105 54-55 0.14544729518776478 1105 56-57 0.2301851917910369 1105 58-59 0.3782386964233879 1105 60-61 0.43280308640806453 1105 62-63 0.22976842180740675 1105 64-65 0.24990425083016987 1105 66-67 0.34095520215910113 1105 68-69 0.32567217033761153 1105 70-71 0.4059836110555679 1105 72-73 0.18381771873554698 1105 74-75 0.16705011160111027 1105 76-77 0.04968446121814196 1105 78-79 0.17700694134970973 1105 80-81 0.2590978495746441 1105 82-83 0.5630978511931701 1105 84-85 0.44810834464535887 1105 86-87 0.5905469210980954 1105 88-89 0.17513250932250557 1105 90-91 0.3687896850863268 1105 92-93 0.3243873966764568 1105 94-95 0.1251180003345489 1105 96-97 0.22938773297092752 1105 98-99 -0.11176279838543124 1105 100-101 -0.012223840877222614 1106 1 0.32233943607034377 1106 2 0.290490346057247 1106 3 0.2515621989238781 1106 4 0.33651495284176747 1106 5 0.3217738883384271 1106 6 0.060969547137595725 1106 7 0.14917312839403252 1106 8 0.3750674691200473 1106 9 0.14556096645016225 1106 10-11 0.21869276470089005 1106 12-13 0.27081413537522536 1106 14-15 0.39835352131672863 1106 16-17 0.3131099355522906 1106 18-19 0.18870389670246368 1106 20-21 0.32659642876581074 1106 22-23 0.4396717705697881 1106 24-25 0.3171424192136598 1106 26-27 0.22141588076997465 1106 28-29 0.21754507240495258 1106 30-31 0.3354808676225147 1106 32-33 0.42535882458316365 1106 34-35 0.21884230942830385 1106 36-37 0.11972942730142933 1106 38-39 0.41661197275291784 1106 40-41 0.4126369249026496 1106 42-43 0.008072844355375253 1106 44-45 0.2443116721897809 1106 46-47 0.18073176661663837 1106 48-49 0.5069319920397888 1106 50-51 0.052272817788164616 1106 52-53 0.41747456392599247 1106 54-55 0.11747473662180141 1106 56-57 -0.11015181936890883 1106 58-59 -0.5667467081902302 1106 60-61 0.07493053521883297 1106 62-63 0.12561743069110065 1106 64-65 0.333907285827479 1106 66-67 -0.1843301192028619 1106 68-69 -0.2328790399729499 1106 70-71 -0.3468190192754328 1106 72-73 0.12740154472903598 1106 74-75 0.051841402844932105 1106 76-77 -0.07904895431303771 1106 78-79 0.18501069294361017 1106 80-81 0.07021985014370458 1106 82-83 0.16624982236069386 1106 84-85 -0.12240581340634193 1106 86-87 0.33955896624368265 1106 88-89 -0.06477243624776463 1106 90-91 0.10740791825420715 1106 92-93 0.09216373423560142 1106 94-95 -0.12162411973753606 1106 96-97 0.28454459385634934 1106 98-99 0.20816280202766535 1106 100-101 -0.49865487372474604 1107 1 0.2824063008548876 1107 2 0.13970650045563104 1107 3 0.053127803211239666 1107 4 0.2359819545279933 1107 5 0.18193591648674357 1107 6 0.14957164740972928 1107 7 0.1894944299617265 1107 8 0.06840144244090851 1107 9 0.22151635653647617 1107 10-11 0.2901185038864895 1107 12-13 0.13675587703173164 1107 14-15 0.1383039779339228 1107 16-17 0.2078260732553474 1107 18-19 0.3365837988678493 1107 20-21 0.32229633861103935 1107 22-23 0.4143658287029197 1107 24-25 0.30107421045529037 1107 26-27 0.3916414346374424 1107 28-29 0.29417557977593134 1107 30-31 0.22811113291126617 1107 32-33 0.331032314073461 1107 34-35 0.44559719698526834 1107 36-37 0.2774230513560596 1107 38-39 0.38906677015511804 1107 40-41 0.1727271631449696 1107 42-43 0.1761363533791993 1107 44-45 0.4093606312361402 1107 46-47 0.08572596499050888 1107 48-49 0.2104407236957968 1107 50-51 0.18412833642850046 1107 52-53 0.15138861638271095 1107 54-55 0.1621159540450421 1107 56-57 0.08101593527656803 1107 58-59 0.04020877372038001 1107 60-61 0.1390854595055231 1107 62-63 0.3127501334975875 1107 64-65 0.3640089603687784 1107 66-67 0.24879048783923707 1107 68-69 0.09552534588944894 1107 70-71 0.46360971672135776 1107 72-73 0.09029931089428445 1107 74-75 0.17623411032611003 1107 76-77 0.2916947807655177 1107 78-79 0.09628337771914275 1107 80-81 -0.20606172489344132 1107 82-83 0.0011267778008630103 1107 84-85 0.09245052330501125 1107 86-87 0.42426375112439274 1107 88-89 0.3602050250608144 1107 90-91 0.5174848002349428 1107 92-93 0.5938750052508475 1107 94-95 0.30581068501061637 1107 96-97 0.5447913507890796 1107 98-99 0.4131375920839311 1107 100-101 -0.3931017992802843 1108 1 0.5067698086342389 1108 2 0.48582000426187477 1108 3 0.4047301584708407 1108 4 0.431981430077478 1108 5 0.5320086243995874 1108 6 0.5111882264031067 1108 7 0.520083006475879 1108 8 0.4828623708772568 1108 9 0.5483364382236147 1108 10-11 0.48442642785705203 1108 12-13 0.5473062719588668 1108 14-15 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-0.447961063223417 2316 62-63 -0.6525184645783497 2316 64-65 -0.5338393175191598 2316 66-67 -0.46897039928715145 2316 68-69 -0.4732535701713587 2316 70-71 -0.6575715273118341 2316 72-73 -0.5911788046965185 2316 74-75 -0.49871264740319887 2316 76-77 -0.4241613182283892 2316 78-79 -0.4468979397516648 2316 80-81 -0.12640486214834468 2316 82-83 -0.009503316933660244 2316 84-85 -0.09758766425465737 2316 86-87 0.07890041852543561 2316 88-89 0.15966525859242608 2316 90-91 0.15427153740171917 2316 92-93 0.3116909705882911 2316 94-95 0.2101444222508313 2316 96-97 -0.24067901713337037 2316 98-99 -0.5474080493936349 2316 100-101 -0.17545565842693733 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3549.0 3 648.0 4 418.0 5 399.0 6 390.0 7 385.0 8 496.0 9 577.0 10 759.0 11 1189.0 12 1863.0 13 2665.0 14 3363.0 15 4892.0 16 6449.0 17 8681.0 18 7362.0 19 4815.0 20 4261.0 21 4115.0 22 4192.0 23 4826.0 24 5286.0 25 6005.0 26 7161.0 27 8521.0 28 10601.0 29 12950.0 30 16399.0 31 21206.0 32 27390.0 33 36327.0 34 48743.0 35 67784.0 36 91814.0 37 122942.0 38 86976.0 39 7152.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.42130462664915 21.069278327408117 15.490651441664124 22.01876560427861 2 15.006886388578856 21.99571582687961 40.819340336826784 22.178057447714743 3 18.51429264281373 25.798263026909556 31.576566563501178 24.11087776677553 4 11.94001312038105 18.563473729701496 38.80445968591922 30.692053463998235 5 12.310064889637868 38.41920509416304 35.55467115224968 13.716058863949407 6 29.256916848566256 37.74090035633664 18.608794612040818 14.393388183056285 7 25.24100112223676 34.47955881484337 22.602345816792514 17.67709424612735 8 24.010059499014567 35.32652959718483 21.481973104207206 19.181437799593393 9 24.70454556052794 17.222083188902758 22.817181202211614 35.25619004835769 10-11 23.014752229233522 27.74190088547573 29.187667537401303 20.055679347889445 12-13 24.36075990686136 26.139052012770815 28.802254477400822 20.697933602967005 14-15 21.325354575480862 27.7105886924422 28.559782397513118 22.404274334563823 16-17 19.641985632174976 30.29778576335676 29.371897255440782 20.688331349027482 18-19 18.920523743239283 28.968080947506763 32.32195582208522 19.789439487168725 20-21 20.828349107969395 27.835800767784452 32.0930482006655 19.24280192358065 22-23 19.783769499218863 28.513201563823753 31.68336455281014 20.019664384147244 24-25 19.649080256313983 28.325213718068614 32.174820874835106 19.850885150782304 26-27 19.597555610483326 28.381205996247296 32.21436133250631 19.80687706076307 28-29 19.07494054188494 29.019912639318523 32.201582440813915 19.703564377982623 30-31 19.787877374623015 28.627226937785654 32.01279420452072 19.572101483070607 32-33 19.34049193006183 28.64728948789995 32.070901975415154 19.941316606623065 34-35 19.921962442482275 28.864569083447332 31.77729759980102 19.436170874269372 36-37 19.643849521720576 28.63132106473619 31.693832772030895 20.03099664151234 38-39 19.25436346963833 28.92751122923175 31.90073203711475 19.917393264015168 40-41 20.016256826779312 28.646129600790772 31.625511717037963 19.71210185539195 42-43 19.496594854515898 28.654586766712566 31.44615332013341 20.402665058638128 44-45 20.00506577347446 28.82199788822511 31.116202783222967 20.056733555077464 46-47 19.932928823900088 28.874327792502047 30.763929196086064 20.4288141875118 48-49 19.722034014197444 28.664436142571205 31.07222111714758 20.541308726083766 50-51 19.873055487137332 29.049998484870855 30.525656575388556 20.551289452603264 52-53 19.953178042320026 29.389208882962976 30.084856057056587 20.572757017660408 54-55 20.03198192353088 28.876817534423694 30.258194202382793 20.833006339662635 56-57 20.166151522356333 29.117463780615342 29.94930917827101 20.767075518757313 58-59 20.27980341498475 29.01509353328542 30.04028827288797 20.66481477884186 60-61 20.135418746809368 28.87920551434337 30.288095069741278 20.697280669105986 62-63 19.71699996969855 28.871146568127376 30.484116211510358 20.927737250663718 64-65 19.91955133982683 28.813424996425884 30.172598661105553 21.094425002641735 66-67 19.818857357744676 28.800312652480244 29.988920416579905 21.39190957319518 68-69 20.02727283322779 28.573149079833254 30.132517997350416 21.267060089588536 70-71 19.96479776197692 28.22846485604383 30.192563235808368 21.614174146170882 72-73 19.91559469914196 27.89423460974787 30.606906905506783 21.58326378560339 74-75 19.97550667077997 28.22724090261648 30.04854372704236 21.74870869956119 76-77 19.729877573094054 28.099057322148425 30.073381549870547 22.097683554886974 78-79 19.844700204108747 28.054269462394497 30.060548894805912 22.040481438690847 80-81 19.95081757048122 28.070347190657667 29.775182685920072 22.20365255294104 82-83 19.988671803980253 27.983208163760025 30.125231730758774 21.902888301500948 84-85 20.077168779121852 27.860011545791963 29.864208744189224 22.198610930896958 86-87 19.831873094411517 27.92718592949206 29.912105945280665 22.328835030815757 88-89 20.30562968512247 27.747970525013482 29.62396233717184 22.322437452692213 90-91 20.40350972635637 27.611971617536195 29.487607930302868 22.49691072580457 92-93 20.563167514205073 27.352307625359014 29.781624165412975 22.302900695022938 94-95 20.370701379444338 27.377656887507285 29.687507285797555 22.564134447250826 96-97 20.31346295978233 27.286836405349092 29.648727814766655 22.750972820101918 98-99 20.75246198330988 27.012073489997817 29.235532132641318 22.999932394050987 100-101 21.07845348556851 26.572764110660867 29.553172127896215 22.795610275874406 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1642.0 1 945.5 2 285.0 3 321.5 4 243.5 5 225.5 6 430.0 7 827.5 8 1090.0 9 1105.5 10 941.5 11 712.5 12 687.5 13 751.5 14 814.0 15 894.0 16 1000.0 17 1120.5 18 1296.0 19 1527.5 20 1785.0 21 2080.0 22 2379.0 23 2823.0 24 3409.0 25 4175.0 26 5262.0 27 6519.0 28 7907.5 29 9500.5 30 11181.0 31 12811.5 32 14688.5 33 16783.0 34 18656.5 35 20399.5 36 22280.0 37 23567.5 38 23699.5 39 23594.0 40 23890.5 41 24168.0 42 24114.0 43 24221.0 44 24256.0 45 23977.0 46 24021.5 47 24633.0 48 25933.5 49 25195.5 50 22386.5 51 20169.5 52 18129.5 53 15835.5 54 13796.0 55 12280.0 56 10636.0 57 8811.0 58 6969.5 59 5374.5 60 4047.0 61 2993.0 62 2224.5 63 1527.5 64 1051.5 65 756.0 66 558.5 67 448.0 68 343.5 69 261.5 70 207.5 71 162.5 72 134.0 73 118.5 74 95.5 75 62.5 76 54.0 77 44.5 78 30.0 79 22.0 80 18.5 81 15.5 82 12.5 83 8.5 84 4.0 85 2.5 86 2.5 87 2.0 88 1.5 89 0.5 90 0.0 91 1.0 92 1.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17077123646766146 2 0.03931312359082652 3 0.03853618438942679 4 0.04304243175754524 5 0.047393291285383755 6 0.05267647785490194 7 0.029989853174029718 8 0.027814423410110464 9 0.03418532486158828 10-11 0.03488457014284804 12-13 0.03247605861850887 14-15 0.027348259889270622 16-17 0.026260545007311 18-19 0.03426301878172825 20-21 0.04218779863600554 22-23 0.03977928711166636 24-25 0.05524037721952106 26-27 0.043819370958944975 28-29 0.037448469507467164 30-31 0.045217861521464495 32-33 0.0417216351151657 34-35 0.042110104715865564 36-37 0.03970159319152639 38-39 0.021909685479472488 40-41 0.02004503139611313 42-43 0.01755882595163398 44-45 0.002563899364619121 46-47 0.008624025135537044 48-49 0.00854633121539707 50-51 0.006448595371617789 52-53 0.007691698093857363 54-55 0.00971174001749667 56-57 0.006526289291757763 58-59 0.009867127857776619 60-61 0.010177903538336511 62-63 0.002719287204899068 64-65 0.005438574409798136 66-67 0.0038846960069986686 68-69 0.00831324945497715 70-71 0.019578867875273288 72-73 0.01678188675023425 74-75 0.017170356350934116 76-77 0.010100209618196538 78-79 0.0033408385660188547 80-81 0.004428553447978482 82-83 0.003729308166718722 84-85 0.004117777767418588 86-87 0.00761400417371739 88-89 0.024551278764231584 90-91 0.03045601669486956 92-93 0.01849115299331366 94-95 0.027348259889270622 96-97 0.029445995733049905 98-99 0.018102683392613797 100-101 0.10659605843204346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 643551.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.3571774149643 #Duplication Level Percentage of deduplicated Percentage of total 1 82.15377188875166 60.26568819751663 2 12.184197984401997 17.875967464016558 3 3.333805065546063 7.336755889805079 4 1.1628262657034518 3.4120661070395424 5 0.5061593764722879 1.8565211590062671 6 0.2320758418110611 1.021465722087672 7 0.12027219620643742 0.6175980184642119 8 0.07364687065137013 0.4322021245143589 9 0.050977338718880244 0.33655983124892647 >10 0.1608650950723182 2.0504892155234384 >50 0.009959437973954797 0.5061311462734237 >100 0.00911173080951125 1.3997766826965492 >500 0.001271404298733898 0.6761830726356886 >1k 0.0010595035822782485 2.2125953691715843 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4545 0.7062377340723579 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3800 0.5904737930637975 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2602 0.40431916040842136 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2025 0.31466037656689216 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1262 0.1960994544332928 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 999 0.1552324524396668 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 994 0.15445551323826706 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 705 0.10954842739736245 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.5538784027994675E-4 0.0 10-11 0.0 0.0 0.0 1.5538784027994675E-4 0.0 12-13 0.0 0.0 0.0 3.107756805598935E-4 0.0 14-15 0.0 0.0 0.0 3.107756805598935E-4 0.0 16-17 0.0 0.0 0.0 3.107756805598935E-4 0.0 18-19 0.0 0.0 0.0 3.107756805598935E-4 0.0 20-21 0.0 0.0 0.0 5.438574409798136E-4 0.0 22-23 0.0 0.0 0.0 0.0011654088020996004 0.0 24-25 0.0 0.0 0.0 0.002719287204899068 0.0 26-27 0.0 0.0 0.0 0.005827044010498003 0.0 28-29 0.0 0.0 0.0 0.013207966423795472 0.0 30-31 0.0 0.0 0.0 0.03643844854564751 0.0 32-33 0.0 0.0 0.0 0.08359865807061134 0.0 34-35 0.0 0.0 0.0 0.1668088465405228 0.0 36-37 0.0 0.0 0.0 0.306191739271635 0.0 38-39 0.0 0.0 0.0 0.47820607846153607 0.0 40-41 0.0 0.0 0.0 0.6399648201929606 0.0 42-43 0.0 0.0 0.0 0.8174177337926598 0.0 44-45 0.0 0.0 0.0 1.061532030872456 0.0 46-47 0.0 0.0 0.0 1.3378116108902014 0.0 48-49 0.0 0.0 0.0 1.6047679204911498 0.0 50-51 0.0 0.0 0.0 1.8763081713803569 0.0 52-53 0.0 0.0 0.0 2.1340965984047884 0.0 54-55 0.0 0.0 0.0 2.4454161364056617 0.0 56-57 0.0 0.0 0.0 2.828136387015171 0.0 58-59 0.0 0.0 0.0 3.206039614576001 0.0 60-61 0.0 0.0 0.0 3.5893814165466296 0.0 62-63 0.0 0.0 0.0 3.9771517719652367 0.0 64-65 0.0 0.0 0.0 4.411849255148388 0.0 66-67 0.0 0.0 0.0 4.904428708835819 0.0 68-69 0.0 0.0 0.0 5.430960405624418 0.0 70-71 0.0 0.0 0.0 5.999058349687903 0.0 72-73 0.0 0.0 0.0 6.567156293751388 0.0 74-75 0.0 0.0 0.0 7.14574291703377 0.0 76-77 0.0 0.0 0.0 7.78827163659135 0.0 78-79 0.0 0.0 0.0 8.497461739629028 0.0 80-81 0.0 0.0 0.0 9.237263247201852 0.0 82-83 0.0 0.0 0.0 9.975433182451741 0.0 84-85 0.0 0.0 0.0 10.73675590590334 0.0 86-87 0.0 0.0 0.0 11.514394352584333 0.0 88-89 0.0 0.0 0.0 12.380526174304755 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGCCG 30 1.2978668E-4 63.36215 3 TCGCCGG 40 4.4758574E-4 29.70794 94-95 TAGGACG 120 5.3578533E-6 27.72094 4 GCTCGGA 70 0.008370274 27.155209 2 GTATCAA 4445 0.0 26.326536 1 GTACATG 2470 0.0 24.844093 1 GGTATCA 3305 0.0 24.180664 1 TACATGG 2465 0.0 24.098183 2 GTCTAGC 165 2.396384E-6 23.064096 1 GTGTAGC 535 0.0 22.228832 1 GTAGCCC 430 0.0 22.103075 3 ACATGGG 2560 0.0 22.090126 3 TAGCCCT 775 0.0 22.074556 4 AGTACTT 3510 0.0 21.926233 12-13 CATGGGG 1020 0.0 21.897215 4 TATCAAC 5345 0.0 21.693686 2 ATCAACG 5425 0.0 21.198582 3 TCAACGC 5440 0.0 21.140131 4 CAACGCA 5490 0.0 20.947596 5 AACGCAG 5570 0.0 20.556623 6 >>END_MODULE