FastQCFastQC Report
Thu 26 May 2016
SRR938318_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938318_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences643551
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42490.6602429333494937No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40270.6257468328073456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27330.4246749674850944No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19340.300520083101417No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15240.2368110685866388No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10070.15647555516190634No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT10070.15647555516190634No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT9300.14451069146035045RNA PCR Primer, Index 39 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.11809475861275952No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7210.11203463284184159No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCA650.005824506729.2489013
GCGTCAG700.00836353327.1596954
GGTATCA42650.024.076811
ATAGGAC2052.6471753E-823.1851063
TAGCCCT6650.022.8713234
GTATCAA54550.021.0032521
GAGTACT37650.020.56277812-13
AGTACTT37500.020.2017312-13
ATAGCCC3800.020.0124073
GGACGTG1200.00476344919.7900966
TATACTG2451.7415914E-719.3997825
GTACTTT41150.018.9292414-15
AGAGTAC51400.018.5734310-11
CCTACTC2051.5791618E-518.5480843
AAGTCGT650.007567068618.2677856-57
GTGTAGC4950.018.247891
GTATAGC4700.018.2070221
CTCCGAC1455.2190444E-818.01581294-95
TATCAAC63950.017.9903262
GTGCTAT1851.5101192E-417.9883171
Produced by FastQC (version 0.11.5)