##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938318_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 643551 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.352601425528047 31.0 31.0 34.0 30.0 34.0 2 31.613618811873497 31.0 31.0 34.0 30.0 34.0 3 31.649953150566155 31.0 31.0 34.0 30.0 34.0 4 35.40993176919933 37.0 35.0 37.0 33.0 37.0 5 35.27593306513392 37.0 35.0 37.0 33.0 37.0 6 35.31962346418543 37.0 35.0 37.0 32.0 37.0 7 35.245528326426346 37.0 35.0 37.0 32.0 37.0 8 35.25448021990487 37.0 35.0 37.0 32.0 37.0 9 36.731146404869236 39.0 35.0 39.0 32.0 39.0 10-11 36.633429984569986 38.0 35.0 39.0 32.0 39.0 12-13 36.52394759700475 38.0 35.0 39.0 32.0 39.0 14-15 37.38980049755186 39.0 36.0 40.5 32.0 41.0 16-17 37.28611174561146 39.0 36.0 40.0 32.0 41.0 18-19 37.45740663910087 39.0 36.0 40.5 32.0 41.0 20-21 37.5443507973727 39.0 36.0 41.0 32.0 41.0 22-23 37.40013845056569 39.0 36.0 41.0 32.0 41.0 24-25 37.27695318630536 39.0 36.0 41.0 31.5 41.0 26-27 37.02574465737758 39.0 36.0 40.0 31.0 41.0 28-29 36.876301955866744 39.0 36.0 40.0 30.5 41.0 30-31 36.57866975577693 38.5 35.0 40.0 30.0 41.0 32-33 36.26327827942153 38.0 35.0 40.0 30.0 41.0 34-35 36.00798926580799 38.0 35.0 40.0 30.0 41.0 36-37 35.92987968319527 38.0 35.0 40.0 30.0 41.0 38-39 35.74756468407321 38.0 35.0 40.0 29.0 41.0 40-41 35.52406025318895 38.0 34.5 40.0 28.0 41.0 42-43 35.22769135624061 38.0 34.0 40.0 27.0 41.0 44-45 34.80390676108032 38.0 33.5 40.0 25.5 41.0 46-47 34.2551033251444 38.0 33.0 40.0 24.0 41.0 48-49 33.76626794146851 38.0 33.0 40.0 22.5 41.0 50-51 33.31220136399446 37.5 32.5 40.0 19.5 41.0 52-53 32.75826546769409 37.0 31.0 40.0 16.0 40.5 54-55 32.387564466530236 37.0 31.0 39.0 13.5 40.0 56-57 31.9881757622939 36.0 31.0 39.0 9.0 40.0 58-59 31.474960026478087 36.0 30.0 39.0 8.0 40.0 60-61 31.026896858213256 35.0 29.0 39.0 7.0 40.0 62-63 30.509683770206244 35.0 28.0 38.0 2.0 40.0 64-65 29.948189032415456 34.0 28.0 38.0 2.0 40.0 66-67 29.381606119794704 34.0 26.5 38.0 2.0 40.0 68-69 28.78307702109079 34.0 26.0 37.0 2.0 39.0 70-71 28.144036758547497 33.0 26.0 36.5 2.0 39.0 72-73 27.649963250775773 32.5 25.0 36.0 2.0 39.0 74-75 27.07973493942205 32.0 23.5 36.0 2.0 38.0 76-77 25.99442079959475 30.5 22.5 34.0 2.0 36.5 78-79 26.649604304864727 32.0 24.0 35.0 2.0 37.0 80-81 26.293156253350553 32.0 22.5 35.0 2.0 37.0 82-83 25.70952340995508 31.0 21.5 34.5 2.0 36.0 84-85 25.052809334458342 31.0 19.0 34.0 2.0 36.0 86-87 24.33681635177321 30.5 16.5 34.0 2.0 35.0 88-89 23.522515698056566 30.0 8.5 34.0 2.0 35.0 90-91 22.805686728790725 29.5 4.0 34.0 2.0 35.0 92-93 21.87014937433086 29.0 2.0 34.0 2.0 35.0 94-95 20.869217824228382 28.5 2.0 33.5 2.0 35.0 96-97 19.627843014772722 26.5 2.0 33.0 2.0 34.0 98-99 18.149102402140624 24.5 2.0 32.0 2.0 34.0 100-101 14.905571586401077 9.5 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46840412646694674 1101 2 0.494954236365448 1101 3 0.35644894322273046 1101 4 0.44696477601029017 1101 5 0.18662875433066972 1101 6 0.2705190761877745 1101 7 0.46360244286313446 1101 8 0.32783271797017477 1101 9 0.5546008732698979 1101 10-11 0.47353509086124035 1101 12-13 0.6361706679773249 1101 14-15 0.6809458984129506 1101 16-17 0.8218396812100437 1101 18-19 0.6512908825312991 1101 20-21 0.5806062445882745 1101 22-23 0.5335115629859288 1101 24-25 0.622919329690653 1101 26-27 0.6984145524332632 1101 28-29 0.7353059639715909 1101 30-31 0.9005393186871871 1101 32-33 0.6374144659964571 1101 34-35 0.6487193583626905 1101 36-37 0.8774766845996496 1101 38-39 0.9768554385227404 1101 40-41 0.9315979038724223 1101 42-43 1.1739409801434348 1101 44-45 1.3601668659058177 1101 46-47 1.4112248359262267 1101 48-49 1.4244407425934398 1101 50-51 1.5757012625920481 1101 52-53 1.4419365444213028 1101 54-55 1.2993335550852763 1101 56-57 1.4293555228453982 1101 58-59 1.9037911382005497 1101 60-61 1.711560533747516 1101 62-63 1.5868009929043971 1101 64-65 1.5095239039139337 1101 66-67 1.3407880547140607 1101 68-69 1.149496047367112 1101 70-71 1.2882481158955912 1101 72-73 1.4398295840528412 1101 74-75 1.3593275812675287 1101 76-77 1.3094825384192674 1101 78-79 1.317658973527191 1101 80-81 1.724012468459545 1101 82-83 1.734067688487773 1101 84-85 2.1011224272137277 1101 86-87 1.7443095739513232 1101 88-89 1.6424250835883214 1101 90-91 1.9809534019514992 1101 92-93 1.8703651011906643 1101 94-95 1.6234624238858935 1101 96-97 1.7533217644554107 1101 98-99 1.3863130144063263 1101 100-101 0.9164153756391862 1102 1 0.48321613565817145 1102 2 0.4504803328732869 1102 3 0.4402481503535043 1102 4 0.3647917083134047 1102 5 0.3832375970951105 1102 6 0.3801042086272375 1102 7 0.4060764978589688 1102 8 0.35393175087172324 1102 9 0.506715612052318 1102 10-11 0.48077949153795885 1102 12-13 0.6081096624979452 1102 14-15 0.6418743634282222 1102 16-17 0.6713674947595223 1102 18-19 0.5053524435241599 1102 20-21 0.3990795925669701 1102 22-23 0.6000204133255451 1102 24-25 0.6177732548983386 1102 26-27 0.5388609832592124 1102 28-29 0.4939012724078182 1102 30-31 0.541686454005216 1102 32-33 0.555421940177709 1102 34-35 0.4798622010766209 1102 36-37 0.4520480554553856 1102 38-39 0.4386777752144084 1102 40-41 0.474616109563911 1102 42-43 0.5802498456334249 1102 44-45 0.9835186321861542 1102 46-47 0.7365575127436443 1102 48-49 0.6718399295237276 1102 50-51 0.6066989142851043 1102 52-53 0.3745679107319333 1102 54-55 0.5984510461844295 1102 56-57 1.009503491433641 1102 58-59 1.260182068180601 1102 60-61 0.8764609200818825 1102 62-63 0.37251167138835584 1102 64-65 0.41644383422224607 1102 66-67 0.41280532613289367 1102 68-69 0.3000300978178352 1102 70-71 0.32427316450302257 1102 72-73 0.2887059318042766 1102 74-75 0.2274019695714422 1102 76-77 0.07476673406100787 1102 78-79 0.3833534916192569 1102 80-81 0.4690986993006412 1102 82-83 0.41843629692652584 1102 84-85 0.5125798925572589 1102 86-87 0.015064314753786334 1102 88-89 0.4185683050614415 1102 90-91 0.10829981510201847 1102 92-93 0.32614244129116443 1102 94-95 0.6862909974788032 1102 96-97 0.3518307157974796 1102 98-99 0.27703341437602447 1102 100-101 0.6195754897719521 1103 1 0.34080875467181926 1103 2 0.3009083539889943 1103 3 0.3105315954479053 1103 4 0.11127004568916021 1103 5 0.21517035022203856 1103 6 0.20575218503852 1103 7 0.35797405813860905 1103 8 0.34770642153325326 1103 9 0.47194365219118595 1103 10-11 0.45211515520847456 1103 12-13 0.4072583760567028 1103 14-15 0.6797019184764963 1103 16-17 0.5937042522617801 1103 18-19 0.3869701255520255 1103 20-21 0.4473330286253727 1103 22-23 0.6423148442952495 1103 24-25 0.5138687511650417 1103 26-27 0.5820649590997959 1103 28-29 0.7147078544214196 1103 30-31 0.9180049968311081 1103 32-33 0.6965309904464192 1103 34-35 0.6382954060036283 1103 36-37 0.7705968260836258 1103 38-39 0.6351656241705044 1103 40-41 0.38597531439579313 1103 42-43 0.7523167120432745 1103 44-45 0.9256156978636128 1103 46-47 0.5891589299691162 1103 48-49 0.7071813952582602 1103 50-51 0.5220327707553594 1103 52-53 0.48153157649499434 1103 54-55 0.5461111974185613 1103 56-57 0.8039808300934581 1103 58-59 0.741171985905428 1103 60-61 0.5855813366890992 1103 62-63 0.8335736393343289 1103 64-65 0.708693085185292 1103 66-67 0.7285351021351723 1103 68-69 0.23865543666788724 1103 70-71 0.21398401246041132 1103 72-73 0.13466232120837418 1103 74-75 0.5490170359147157 1103 76-77 0.7420778102963261 1103 78-79 0.7676724624662619 1103 80-81 0.5510243187423107 1103 82-83 0.6557169361047244 1103 84-85 0.33589990967810834 1103 86-87 0.16505955896361257 1103 88-89 0.07299951255656723 1103 90-91 0.1123981172849291 1103 92-93 0.02579764650350924 1103 94-95 -0.03360128387374672 1103 96-97 0.4603994607444548 1103 98-99 0.3508171210388902 1103 100-101 0.07287957499571363 1104 1 0.37108663269419395 1104 2 0.2378264341044769 1104 3 0.333077607717847 1104 4 0.29937903161481216 1104 5 0.2569152076742256 1104 6 0.33247538965778745 1104 7 0.3754677427290076 1104 8 0.24908678963141284 1104 9 0.592955922129839 1104 10-11 0.36541811442897654 1104 12-13 0.3437343775002262 1104 14-15 0.48645940674787624 1104 16-17 0.5056584204321126 1104 18-19 0.5407548603048227 1104 20-21 0.6318828301410733 1104 22-23 0.5991084206214836 1104 24-25 0.6534663693534242 1104 26-27 0.6393682792008448 1104 28-29 0.7061504744142084 1104 30-31 0.609037115199925 1104 32-33 0.6297094458776655 1104 34-35 0.6622037423442961 1104 36-37 0.7924436322907766 1104 38-39 0.5983106981510247 1104 40-41 0.9665734739050009 1104 42-43 1.0044233522453752 1104 44-45 0.5493075058751558 1104 46-47 0.57255842473635 1104 48-49 1.055241662309868 1104 50-51 1.5500641673630753 1104 52-53 1.5895701907136939 1104 54-55 1.8467923594539286 1104 56-57 1.7653801444204191 1104 58-59 1.6124278502143348 1104 60-61 1.5268029173459041 1104 62-63 1.7123087530117012 1104 64-65 2.0586975961921503 1104 66-67 1.9911568993203055 1104 68-69 1.7499103987754303 1104 70-71 1.3447987002987354 1104 72-73 1.5141733280651017 1104 74-75 1.4163212582171312 1104 76-77 1.230552187777377 1104 78-79 1.2552356165622562 1104 80-81 1.1500950513298243 1104 82-83 1.3773111259278927 1104 84-85 1.1846729158130778 1104 86-87 0.9694532796420674 1104 88-89 1.2537826233468934 1104 90-91 1.2544181461553698 1104 92-93 1.7656659251032885 1104 94-95 1.4637191780533563 1104 96-97 1.517174993043266 1104 98-99 1.3770847139656297 1104 100-101 1.0765921336388011 1105 1 0.37805367483019836 1105 2 0.35478513247744203 1105 3 0.1850279623277764 1105 4 0.25217419223225335 1105 5 0.3119527546078871 1105 6 0.11330559407998209 1105 7 0.1657994073097342 1105 8 0.3513917541704217 1105 9 0.17859422000217506 1105 10-11 0.22488307062421597 1105 12-13 0.2950798092899589 1105 14-15 0.4725357521796738 1105 16-17 0.37436930904287635 1105 18-19 0.5006682937633116 1105 20-21 0.48482922146355634 1105 22-23 0.49030575061731696 1105 24-25 0.5680783843721926 1105 26-27 0.6447656509154882 1105 28-29 0.7013893146311432 1105 30-31 0.710497273731427 1105 32-33 0.703483955681591 1105 34-35 0.6302992784310746 1105 36-37 0.6296828896958644 1105 38-39 0.5682835809211539 1105 40-41 0.5813784384440055 1105 42-43 0.5582145495753679 1105 44-45 0.599797701953591 1105 46-47 0.2938558798886248 1105 48-49 0.023827394475070207 1105 50-51 0.22136162251534586 1105 52-53 0.4582393605092676 1105 54-55 0.8356273615398706 1105 56-57 0.7496156471321385 1105 58-59 0.5169856307733696 1105 60-61 0.6356890249804437 1105 62-63 1.1711427121272635 1105 64-65 0.7543953433761281 1105 66-67 0.4367868534296129 1105 68-69 0.2551513249331272 1105 70-71 -0.12669170437377275 1105 72-73 0.30021838438550574 1105 74-75 0.48557449142531084 1105 76-77 0.4070279908645702 1105 78-79 0.40129840337911205 1105 80-81 0.5413236704370412 1105 82-83 0.7049446261364842 1105 84-85 0.9967296534017258 1105 86-87 0.8670022992499113 1105 88-89 0.6829753642730552 1105 90-91 0.8232594060635883 1105 92-93 1.2785257498842668 1105 94-95 1.072271535166415 1105 96-97 0.8762905541613293 1105 98-99 0.9564495450044284 1105 100-101 0.5642068603805619 1106 1 0.19733433831433445 1106 2 0.16358030177338634 1106 3 0.2704104457564789 1106 4 0.16986675121974315 1106 5 0.055868533432459344 1106 6 0.176968877989232 1106 7 0.03690686518334019 1106 8 0.1336707420831189 1106 9 0.14759930994558346 1106 10-11 0.36152750172029613 1106 12-13 0.10466218101390723 1106 14-15 0.2646890195439582 1106 16-17 -0.11993374808461965 1106 18-19 0.07557371355191833 1106 20-21 0.0979576924782819 1106 22-23 0.27877802992137646 1106 24-25 0.18540632215475483 1106 26-27 0.19811205159836476 1106 28-29 0.12371340597613312 1106 30-31 0.2585345367539844 1106 32-33 0.09778806662760076 1106 34-35 0.10645031170012231 1106 36-37 -0.32540336534929537 1106 38-39 0.07826218840536825 1106 40-41 -0.022672439913726805 1106 42-43 0.2953321451830533 1106 44-45 0.03327962369702675 1106 46-47 -0.30269286448662314 1106 48-49 0.10894326182039293 1106 50-51 -0.0029731545493447697 1106 52-53 -0.014048374097079375 1106 54-55 0.2032024197633433 1106 56-57 0.2635850653189422 1106 58-59 0.30092754429702495 1106 60-61 0.0196794141556218 1106 62-63 0.22607721730678065 1106 64-65 -0.5193867058793451 1106 66-67 0.009273759734604425 1106 68-69 -0.5544500155240222 1106 70-71 -0.6573525539622587 1106 72-73 -0.3186428593311028 1106 74-75 -0.024051086857596715 1106 76-77 -0.23303818893794315 1106 78-79 -0.2239215812351354 1106 80-81 -0.977911678851104 1106 82-83 -0.2140630983532006 1106 84-85 -0.40239989250787644 1106 86-87 -0.19565248034394855 1106 88-89 -0.35073839754288727 1106 90-91 -0.34610082071815285 1106 92-93 -0.17281628798997062 1106 94-95 -0.24978488627668227 1106 96-97 0.13511048819228932 1106 98-99 -0.24536196901662422 1106 100-101 -0.3637572676373111 1107 1 0.2037369962103881 1107 2 0.2974100428288651 1107 3 0.2752124391785209 1107 4 0.18825874080186367 1107 5 0.012681896372171764 1107 6 0.029302884700754817 1107 7 0.15641618952345482 1107 8 0.11256993569882923 1107 9 0.3761019895455675 1107 10-11 0.2847175195842979 1107 12-13 0.25678791352137154 1107 14-15 0.3395988647782815 1107 16-17 0.426957164648897 1107 18-19 0.2279822231600761 1107 20-21 0.37578567217675385 1107 22-23 0.18671582313304924 1107 24-25 0.19041283333226744 1107 26-27 0.2809000042504124 1107 28-29 0.6037958808914041 1107 30-31 0.47970796781063996 1107 32-33 0.5770326448201644 1107 34-35 0.6369641521261897 1107 36-37 0.5453435001030655 1107 38-39 0.514495430470916 1107 40-41 0.592556619972413 1107 42-43 0.5397339933557461 1107 44-45 0.8384681139386103 1107 46-47 0.8310680083450706 1107 48-49 0.5759042531889094 1107 50-51 0.5907366723201122 1107 52-53 0.6466587564770734 1107 54-55 0.6610686317606067 1107 56-57 0.7184617703292062 1107 58-59 0.45070445299027995 1107 60-61 0.5254810402804679 1107 62-63 0.7378870742278316 1107 64-65 0.6408853027354411 1107 66-67 0.7242699198094016 1107 68-69 0.752058449006757 1107 70-71 0.6882636705564984 1107 72-73 0.5354472870869138 1107 74-75 0.6427174908674971 1107 76-77 0.4453159022519344 1107 78-79 0.5058635081683391 1107 80-81 0.7237814359960488 1107 82-83 0.4482050452933919 1107 84-85 0.6377628034535228 1107 86-87 0.5079199022461651 1107 88-89 0.143422411846565 1107 90-91 0.3909102148929797 1107 92-93 0.03836916370210375 1107 94-95 0.03424958122586119 1107 96-97 0.5723798839884076 1107 98-99 -0.14628772489755804 1107 100-101 0.07676563000363856 1108 1 0.2670763156961087 1108 2 0.23037573105097664 1108 3 0.24068015368672846 1108 4 0.20226196696717125 1108 5 0.16886087703107222 1108 6 0.18430582480214497 1108 7 0.06004840667775113 1108 8 0.2889382747799232 1108 9 0.19157685565176052 1108 10-11 0.2472520856412217 1108 12-13 0.2786062470401163 1108 14-15 0.409229603935664 1108 16-17 0.22118383852014745 1108 18-19 0.27237874748345803 1108 20-21 0.33767262130505316 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8.0 4 19.0 5 38.0 6 92.0 7 251.0 8 491.0 9 907.0 10 1475.0 11 2317.0 12 3166.0 13 4376.0 14 5428.0 15 7508.0 16 9340.0 17 11286.0 18 10274.0 19 8271.0 20 8025.0 21 8374.0 22 8958.0 23 10023.0 24 11427.0 25 13006.0 26 15330.0 27 17938.0 28 20850.0 29 24625.0 30 28770.0 31 33594.0 32 39399.0 33 46982.0 34 56506.0 35 68537.0 36 77858.0 37 67693.0 38 20092.0 39 313.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.58890465217101 22.897557140171145 16.38942800004351 21.12411020761434 2 15.835372318100276 23.800470702353824 38.853619029557244 21.510537949988652 3 18.883381244658533 26.855566778028123 32.22406961386062 22.036982363452722 4 12.827732378630444 20.285571772866486 37.74712493648522 29.139570912017852 5 12.102400139336844 39.25706714605176 35.40213297337982 13.238399741231577 6 28.870439172652983 39.14716937740754 17.772795007699468 14.209596442240008 7 24.894685891250266 35.08144653648274 22.271894535165046 17.751973037101955 8 24.12023289529501 36.20956225691515 20.97176447554273 18.69844037224711 9 24.52921369091183 17.35216012406165 22.25247105513005 35.86615512989646 10-11 23.138414826486166 28.491137454529635 28.64800147929224 19.72244623969196 12-13 24.486093565234146 26.48111804658838 28.611485336826455 20.42130305135102 14-15 21.20741013532727 28.341421270419904 28.070114101291114 22.38105449296171 16-17 19.485557477185182 30.967320383310724 29.071122568374534 20.47599957112956 18-19 18.895938317242923 29.37645967452463 32.141741680146566 19.585860328085886 20-21 20.659512610500176 28.028936323616932 32.196671281685525 19.114879784197367 22-23 19.824069887235044 28.737116405692788 31.780542645415828 19.65827106165634 24-25 19.658286334949626 28.616324593019506 32.31385881916027 19.411530252870598 26-27 19.58724357151404 28.574070361754334 32.5018686781569 19.336817388574723 28-29 19.022890182751638 29.181525628893436 32.355633042291906 19.439951146063017 30-31 19.807909551845928 28.58879871214558 32.28827241353055 19.315019322477937 32-33 19.391081670294973 28.806108606777087 32.24033526480419 19.562474458123756 34-35 19.916516895926627 28.99064233970257 32.072694948666125 19.020145815704677 36-37 19.747323636606605 28.690366956439316 31.95802038845436 19.604289018499713 38-39 19.357821260302583 28.83052485672729 32.24063288621474 19.571020996755387 40-41 20.04470713099718 28.723593539609787 31.84866395295262 19.383035376440414 42-43 19.693216833527828 28.6947847017088 31.610801974674814 20.001196490088553 44-45 20.07472607044824 28.858185954326814 31.28472435341127 19.782363621813673 46-47 20.312842338835615 28.805953218936803 30.838270781958226 20.042933660269348 48-49 20.130339320426817 28.708214267400717 30.961182563619666 20.200263848552797 50-51 20.094833198922853 29.08246588071497 30.532389818367157 20.290311101995023 52-53 20.24524862831384 29.43791556535535 30.089379085729025 20.227456720601786 54-55 20.263351311706455 29.1186712475002 29.99016394971028 20.627813491083067 56-57 20.439716510424194 29.331319506923304 29.61210533430917 20.616858648343335 58-59 20.411902087014084 29.46495304956406 29.64427061724712 20.47887424617474 60-61 20.402811898357704 29.545599338669355 29.584057829138636 20.467530933834304 62-63 19.941698482326963 29.458349066352163 29.796239925040908 20.80371252627997 64-65 20.000901249473625 29.463787640761964 29.505820051557684 21.02949105820673 66-67 20.02936834744775 29.203946857276048 29.385906300986715 21.380778494289487 68-69 20.073809912282748 29.055155428136338 29.375956615310507 21.495078044270407 70-71 20.067531712789773 28.71636136769588 29.450026765617878 21.766080153896475 72-73 19.865869216270347 28.433643953626053 29.823588184930177 21.87689864517342 74-75 19.881796469899044 28.676437454063468 29.26714432888769 22.174621747149796 76-77 19.473437225643345 28.387882234663607 29.769590910432896 22.369089629260152 78-79 19.501251649053454 28.37459657431967 29.66493720000435 22.45921457662252 80-81 19.380427975839922 28.260592565197868 29.631290003278732 22.727689455683475 82-83 19.44442286266582 28.10696033079169 29.764803503085247 22.683813303457246 84-85 19.37983231277374 28.140794490299047 29.546657094307164 22.932716102620045 86-87 19.109017126456695 28.09327831443904 29.59187241127737 23.20583214782689 88-89 19.28137785505733 27.997547979880384 29.22417959105028 23.496894574012007 90-91 19.294734282213508 27.651059273802876 29.324328839114848 23.72987760486877 92-93 19.11634567049469 27.62835767900694 29.508960932710842 23.74633571778753 94-95 18.94162234228523 27.600454354044977 29.444597242487387 24.013326061182408 96-97 18.609014670166 27.53449221584614 29.514444076693223 24.342049037294636 98-99 18.847161178493373 27.261263879058443 29.240362644423396 24.651212298024785 100-101 18.69750804520543 26.99063477486633 29.549406340756214 24.762450839172033 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1653.0 1 962.5 2 287.5 3 308.0 4 244.0 5 202.0 6 413.0 7 746.0 8 991.5 9 1066.0 10 865.5 11 639.0 12 597.5 13 651.5 14 711.0 15 778.5 16 897.5 17 1003.0 18 1143.0 19 1347.5 20 1689.5 21 2025.0 22 2351.5 23 2802.5 24 3437.5 25 4262.5 26 5324.0 27 6608.5 28 7930.5 29 9598.5 30 11405.5 31 13130.5 32 15065.0 33 17124.0 34 19052.0 35 20799.5 36 22363.5 37 23472.0 38 24017.0 39 24217.0 40 24407.0 41 24477.5 42 24421.0 43 24476.0 44 24554.5 45 24607.5 46 24712.0 47 25349.0 48 26266.0 49 25297.0 50 22724.5 51 20283.0 52 17728.0 53 15247.5 54 13304.0 55 11632.0 56 9839.5 57 8077.0 58 6409.5 59 4987.0 60 3816.5 61 2833.0 62 2007.0 63 1389.0 64 1016.5 65 692.0 66 453.0 67 313.0 68 240.0 69 173.5 70 116.5 71 93.5 72 66.0 73 47.0 74 43.0 75 30.0 76 23.0 77 19.5 78 9.0 79 3.0 80 3.0 81 3.0 82 2.0 83 0.5 84 0.0 85 0.0 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.1077568055989344E-4 2 0.03993467495194631 3 1.5538784027994672E-4 4 0.0 5 0.0784708593413731 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.769392013997336E-5 26-27 0.007458616333437444 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010488679218896403 36-37 7.769392013997336E-5 38-39 0.003573920326438775 40-41 0.004583941288258429 42-43 3.1077568055989344E-4 44-45 7.769392013997336E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.5538784027994672E-4 68-69 9.323270416796805E-4 70-71 2.3308176041992012E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0015538784027994672 82-83 6.215513611197869E-4 84-85 0.004894716968818322 86-87 0.0022531236840592273 88-89 0.0 90-91 0.0031854507257389076 92-93 0.0011654088020996004 94-95 0.0 96-97 0.0 98-99 7.769392013997336E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 643551.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.53161659239723 #Duplication Level Percentage of deduplicated Percentage of total 1 82.73071470310455 60.83323193963685 2 11.88895591816167 17.48428296516352 3 3.1937926139559822 7.045342018951242 4 1.0805456302698728 3.178170679823781 5 0.4779057003829173 1.7570589363938869 6 0.21552918330031445 0.9508925562546735 7 0.11970966155900474 0.616171145531366 8 0.06392136723867532 0.3760193174284877 9 0.04512373576107418 0.29862191134799554 >10 0.1580103322022761 1.9724541208665398 >50 0.011838641993429214 0.5799790078462079 >100 0.01141569169420467 1.7946366085469128 >500 0.0010570084900074289 0.5512153671129815 >1k 0.0014798118860104004 2.5619234250956144 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4249 0.6602429333494937 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4027 0.6257468328073456 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2733 0.4246749674850944 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1934 0.300520083101417 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1524 0.2368110685866388 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1007 0.15647555516190634 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 1007 0.15647555516190634 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 930 0.14451069146035045 RNA PCR Primer, Index 39 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 760 0.11809475861275952 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 721 0.11203463284184159 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.107756805598935E-4 0.0 10-11 0.0 0.0 0.0 4.661635208398402E-4 0.0 12-13 0.0 0.0 0.0 4.661635208398402E-4 0.0 14-15 0.0 0.0 0.0 4.661635208398402E-4 0.0 16-17 0.0 0.0 0.0 4.661635208398402E-4 0.0 18-19 0.0 0.0 0.0 4.661635208398402E-4 0.0 20-21 0.0 0.0 0.0 6.992452812597603E-4 0.0 22-23 0.0 0.0 0.0 0.0013207966423795473 0.0 24-25 0.0 0.0 0.0 0.002952368965318988 0.0 26-27 0.0 0.0 0.0 0.005904737930637976 0.0 28-29 0.0 0.0 0.0 0.013285660343935446 0.0 30-31 0.0 0.0 0.0 0.035894591104667695 0.0 32-33 0.0 0.0 0.0 0.0819670857476719 0.0 34-35 0.0 0.0 0.0 0.16323492621408403 0.0 36-37 0.0 0.0 0.0 0.3009862466222568 0.0 38-39 0.0 0.0 0.0 0.4704366864475387 0.0 40-41 0.0 0.0 0.0 0.630175386255324 0.0 42-43 0.0 0.0 0.0 0.8051420944105441 0.0 44-45 0.0 0.0 0.0 1.0449055319625018 0.0 46-47 0.0 0.0 0.0 1.3170673342128285 0.0 48-49 0.0 0.0 0.0 1.5797504782060785 0.0 50-51 0.0 0.0 0.0 1.8445313580431075 0.0 52-53 0.0 0.0 0.0 2.095793495775782 0.0 54-55 0.0 0.0 0.0 2.3989551721619575 0.0 56-57 0.0 0.0 0.0 2.7698659469101905 0.0 58-59 0.0 0.0 0.0 3.134250432366666 0.0 60-61 0.0 0.0 0.0 3.5022865320697196 0.0 62-63 0.0 0.0 0.0 3.876771227144391 0.0 64-65 0.0 0.0 0.0 4.292822169493949 0.0 66-67 0.0 0.0 0.0 4.770717472274924 0.0 68-69 0.0 0.0 0.0 5.28093344583413 0.0 70-71 0.0 0.0 0.0 5.829452522022342 0.0 72-73 0.0 0.0 0.0 6.378670843491813 0.0 74-75 0.0 0.0 0.0 6.941951764506621 0.0 76-77 0.0 0.0 0.0 7.566222412831307 0.0 78-79 0.0 0.0 0.0 8.25466823919161 0.0 80-81 0.0 0.0 0.0 8.969219222718946 0.0 82-83 0.0 0.0 0.0 9.677865468315643 0.0 84-85 0.0 0.0 0.0 10.408809868992511 0.0 86-87 0.0 0.0 0.0 11.153428399614018 0.0 88-89 0.0 0.0 0.0 11.974808523333815 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCGTCA 65 0.0058245067 29.248901 3 GCGTCAG 70 0.008363533 27.159695 4 GGTATCA 4265 0.0 24.07681 1 ATAGGAC 205 2.6471753E-8 23.185106 3 TAGCCCT 665 0.0 22.871323 4 GTATCAA 5455 0.0 21.003252 1 GAGTACT 3765 0.0 20.562778 12-13 AGTACTT 3750 0.0 20.20173 12-13 ATAGCCC 380 0.0 20.012407 3 GGACGTG 120 0.004763449 19.790096 6 TATACTG 245 1.7415914E-7 19.399782 5 GTACTTT 4115 0.0 18.92924 14-15 AGAGTAC 5140 0.0 18.57343 10-11 CCTACTC 205 1.5791618E-5 18.548084 3 AAGTCGT 65 0.0075670686 18.26778 56-57 GTGTAGC 495 0.0 18.24789 1 GTATAGC 470 0.0 18.207022 1 CTCCGAC 145 5.2190444E-8 18.015812 94-95 TATCAAC 6395 0.0 17.990326 2 GTGCTAT 185 1.5101192E-4 17.988317 1 >>END_MODULE