FastQCFastQC Report
Thu 26 May 2016
SRR938317_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938317_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647794
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46110.7118003562861033No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40110.6191783190335199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26780.4134030262706972No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19770.30518961274726225No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13110.2023791513968947No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT10330.15946427413653105No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.1398592762514009No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7430.11469695613111576No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7080.1092940039580484No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAC300.003952550231.6643590-91
GGTATCA33950.029.04031
GGTCGCG605.3467484E-627.73294-95
GTATCAA46700.026.9251161
TCGCCGG458.903671E-426.41142794-95
GCCGTAT906.6393113E-926.41142794-95
TAGCCCT7900.023.4530854
TATCAAC54050.023.2923132
TCAACGC54150.023.2492984
CAACGCA54500.023.099995
ATCAACG54400.023.0551243
GTACATG26500.022.8259261
GTGTAGC5850.022.796741
AACGCAG55450.022.704236
GAGTACT34800.022.58814612-13
GTATAGC5500.022.515481
ATAGCCC3800.022.5035673
CGCCGTA753.013665E-522.18559894-95
TACATGG26850.022.11712
GTAGCCC5200.021.9265523
Produced by FastQC (version 0.11.5)