##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938317_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 647794 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.858919656557486 33.0 31.0 34.0 31.0 34.0 2 32.04632799933312 33.0 31.0 34.0 30.0 34.0 3 32.099861993164495 34.0 31.0 34.0 30.0 34.0 4 35.75207241808353 37.0 35.0 37.0 35.0 37.0 5 35.775127586856314 37.0 35.0 37.0 35.0 37.0 6 35.81967415567294 37.0 35.0 37.0 35.0 37.0 7 35.78028972173253 37.0 35.0 37.0 35.0 37.0 8 35.769601138633575 37.0 35.0 37.0 35.0 37.0 9 37.40461782603729 39.0 37.0 39.0 35.0 39.0 10-11 37.315776311605234 39.0 37.0 39.0 35.0 39.0 12-13 37.21905883660546 39.0 37.0 39.0 34.5 39.0 14-15 38.39607035569949 40.0 38.0 41.0 34.0 41.0 16-17 38.27717684943053 40.0 38.0 41.0 34.0 41.0 18-19 38.23151572876563 40.0 38.0 41.0 34.0 41.0 20-21 38.256382430217016 40.0 38.0 41.0 34.0 41.0 22-23 38.31719034137396 40.0 38.0 41.0 34.0 41.0 24-25 38.275441729932666 40.0 38.0 41.0 34.0 41.0 26-27 38.13202962670232 40.0 38.0 41.0 34.0 41.0 28-29 37.99285806907751 40.0 38.0 41.0 34.0 41.0 30-31 37.762579616359524 40.0 37.5 41.0 33.5 41.0 32-33 37.474386147448115 40.0 36.5 41.0 33.0 41.0 34-35 37.25561752655937 39.0 36.0 41.0 33.0 41.0 36-37 37.050508340614456 39.0 36.0 41.0 32.5 41.0 38-39 36.79900709176065 39.0 35.5 40.0 31.5 41.0 40-41 36.714082717654065 39.0 36.0 41.0 31.0 41.0 42-43 36.48886065631976 39.0 35.5 41.0 31.0 41.0 44-45 36.15333423897103 39.0 35.0 40.5 30.0 41.0 46-47 35.71399086746713 39.0 35.0 40.0 28.5 41.0 48-49 35.32362911666363 39.0 35.0 40.0 27.0 41.0 50-51 34.579698175654606 38.5 34.5 39.5 25.0 40.5 52-53 34.62433891020911 38.0 35.0 40.0 26.0 40.5 54-55 34.77338552070566 39.0 35.0 40.0 24.5 41.0 56-57 34.53882947356721 38.0 34.5 40.0 23.5 41.0 58-59 34.239056706298605 38.0 34.0 40.0 22.5 41.0 60-61 33.887681114675345 38.0 34.0 40.0 20.0 41.0 62-63 33.44970160266998 38.0 33.0 40.0 17.5 41.0 64-65 33.08652519165043 37.0 33.0 40.0 13.0 41.0 66-67 32.63988551916195 37.0 32.0 39.0 8.0 41.0 68-69 32.128895914441934 36.0 31.5 39.0 6.5 40.0 70-71 31.595863036706113 36.0 31.0 39.0 2.0 40.0 72-73 31.0471916998305 35.0 30.5 38.0 2.0 40.0 74-75 30.443926618647286 35.0 30.0 37.5 2.0 39.5 76-77 29.799412313173633 34.5 29.0 37.0 2.0 39.0 78-79 29.11200705779924 34.0 28.5 36.0 2.0 39.0 80-81 28.435046480825697 34.0 27.5 36.0 2.0 37.5 82-83 27.776212036542482 33.5 26.0 35.0 2.0 37.0 84-85 27.094311617582132 33.0 25.5 35.0 2.0 36.5 86-87 26.42385310762372 32.5 24.5 35.0 2.0 36.0 88-89 25.7205624010102 32.0 21.0 35.0 2.0 36.0 90-91 24.948331568368957 31.5 18.5 34.5 2.0 35.0 92-93 24.146403795033606 31.0 9.0 34.0 2.0 35.0 94-95 23.288652719846127 31.0 2.0 34.0 2.0 35.0 96-97 22.238368987672008 30.5 2.0 34.0 2.0 35.0 98-99 21.00222061334313 29.5 2.0 34.0 2.0 35.0 100-101 17.777827519242226 23.5 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.37242052736484155 1101 2 0.46140495183901464 1101 3 0.4788880289189805 1101 4 0.35694253185421587 1101 5 0.48140056371301654 1101 6 0.28970157672942776 1101 7 0.27082972564697627 1101 8 0.13398693317055432 1101 9 0.229902511500363 1101 10-11 0.4470878241040026 1101 12-13 0.4881192472176821 1101 14-15 0.6185409478737256 1101 16-17 0.7169394729972609 1101 18-19 0.6539828207475011 1101 20-21 0.642043499031729 1101 22-23 0.6065289404073582 1101 24-25 0.7398830313300309 1101 26-27 0.7266988826804521 1101 28-29 0.829636671558859 1101 30-31 0.6579300048460937 1101 32-33 0.5925832525458006 1101 34-35 0.8140982694258057 1101 36-37 0.7652841292982231 1101 38-39 0.7590768184844947 1101 40-41 0.7494787988824001 1101 42-43 0.5643079316406627 1101 44-45 0.49376030989679975 1101 46-47 0.651258430740647 1101 48-49 0.845916749448584 1101 50-51 0.7343817280642071 1101 52-53 0.7852415039715979 1101 54-55 0.9960055924527964 1101 56-57 0.861759475087311 1101 58-59 1.1017424275028844 1101 60-61 1.0734148481932522 1101 62-63 1.0813526003444949 1101 64-65 1.1519711283094765 1101 66-67 1.2475864266746584 1101 68-69 1.301257586868438 1101 70-71 1.6163859427836087 1101 72-73 1.6261473560421003 1101 74-75 1.6059021646436733 1101 76-77 1.725326566206622 1101 78-79 1.8658929782195557 1101 80-81 2.047689990252735 1101 82-83 2.2462736108495562 1101 84-85 2.324664920511214 1101 86-87 2.6075485630067554 1101 88-89 2.8154573677538437 1101 90-91 2.909415934582846 1101 92-93 3.042170040995945 1101 94-95 3.373607059044719 1101 96-97 3.566209305645245 1101 98-99 3.8979699019180565 1101 100-101 4.1275907071081726 1102 1 0.5130588187732705 1102 2 0.46063784769513205 1102 3 0.3617033770779301 1102 4 0.34774487721817593 1102 5 0.28048763383088726 1102 6 0.34532042470609525 1102 7 0.49000884826212143 1102 8 0.48759915822827793 1102 9 0.5338352493785976 1102 10-11 0.490227938334165 1102 12-13 0.5107070464642547 1102 14-15 0.5656297496722473 1102 16-17 0.519763327657607 1102 18-19 0.5263093488797139 1102 20-21 0.573395273244877 1102 22-23 0.5571494593039432 1102 24-25 0.5361713105863259 1102 26-27 0.6222398050282507 1102 28-29 0.6326542010278118 1102 30-31 0.7699272098527814 1102 32-33 0.6237573016404667 1102 34-35 0.6041395867183041 1102 36-37 0.8219586948590489 1102 38-39 0.8779058074260391 1102 40-41 1.0377390674448037 1102 42-43 1.1472159399041217 1102 44-45 1.3321405808896358 1102 46-47 1.14339751203849 1102 48-49 1.0883444441866956 1102 50-51 1.1437836906356011 1102 52-53 1.0639509461881502 1102 54-55 1.119281506901693 1102 56-57 1.2636916661190298 1102 58-59 1.4289161424135628 1102 60-61 1.2927724554359301 1102 62-63 1.5907970954228716 1102 64-65 1.452820259431114 1102 66-67 1.5400429827568018 1102 68-69 1.5930191921667713 1102 70-71 1.6117871154655887 1102 72-73 1.4603503272415175 1102 74-75 1.4001777449085893 1102 76-77 1.7451307647726644 1102 78-79 2.0922342712077686 1102 80-81 2.439657422504524 1102 82-83 2.289398534898652 1102 84-85 2.2771500341337685 1102 86-87 2.5130732318608047 1102 88-89 2.9041680251848803 1102 90-91 2.903784174952275 1102 92-93 2.589654547011257 1102 94-95 3.1660826028532014 1102 96-97 3.7749793566842555 1102 98-99 4.257661996947828 1102 100-101 4.335772138630833 1103 1 0.546387763832076 1103 2 0.6065260344600958 1103 3 0.508505787703406 1103 4 0.3705346496129991 1103 5 0.2594751126208976 1103 6 0.45156055108839865 1103 7 0.38241567056625314 1103 8 0.3874294782797634 1103 9 0.4938507546152735 1103 10-11 0.4534980805142581 1103 12-13 0.5328129794114105 1103 14-15 0.6471200077027888 1103 16-17 0.8195629297873808 1103 18-19 0.753757274688617 1103 20-21 0.8384763100478736 1103 22-23 0.7376721028005235 1103 24-25 0.7208006476643192 1103 26-27 0.7701758532407581 1103 28-29 0.7684127874183133 1103 30-31 0.8738013248840844 1103 32-33 0.9194172981298436 1103 34-35 0.8502449930725291 1103 36-37 0.8138952404093374 1103 38-39 0.7718082999659757 1103 40-41 0.9983213914749953 1103 42-43 1.039023031355761 1103 44-45 0.8996541845406725 1103 46-47 1.1045466548622045 1103 48-49 0.8341646126964406 1103 50-51 1.0289864289189126 1103 52-53 1.1771574583875477 1103 54-55 1.301442439556304 1103 56-57 1.3308359233304259 1103 58-59 1.689349265109719 1103 60-61 1.6180789032739682 1103 62-63 1.7394425813511418 1103 64-65 1.797804461642805 1103 66-67 1.5786049451931774 1103 68-69 1.768583085443936 1103 70-71 1.6918548411691745 1103 72-73 1.8778983057097172 1103 74-75 1.8094574827823209 1103 76-77 2.135968778932167 1103 78-79 2.302977498637407 1103 80-81 2.406189515418923 1103 82-83 2.7246093183519307 1103 84-85 2.7212105045567974 1103 86-87 2.7459626180874785 1103 88-89 3.1674279280577338 1103 90-91 3.347420445504028 1103 92-93 3.4583025196284254 1103 94-95 3.7321956155499443 1103 96-97 4.113900426253032 1103 98-99 4.45788799308615 1103 100-101 5.170919292103424 1104 1 0.4595552918251862 1104 2 0.3045772833834377 1104 3 0.2683930974938207 1104 4 0.2612359128917845 1104 5 0.31644826794676106 1104 6 0.23789776039072308 1104 7 0.2768672927310547 1104 8 0.24583099995648183 1104 9 0.28528414537036895 1104 10-11 0.2834399409793704 1104 12-13 0.2946562179988419 1104 14-15 0.34681316015756636 1104 16-17 0.4826855970282651 1104 18-19 0.6037070306461274 1104 20-21 0.41883986158390485 1104 22-23 0.46377029401498504 1104 24-25 0.4504338661541212 1104 26-27 0.3492204986613743 1104 28-29 0.35633986177948884 1104 30-31 0.33505060711205203 1104 32-33 0.46409860734603114 1104 34-35 0.5221334512981954 1104 36-37 0.7112628114687638 1104 38-39 0.6057337952286801 1104 40-41 0.7492365508203847 1104 42-43 0.8321970192971904 1104 44-45 0.5965480260566096 1104 46-47 0.6618428076040885 1104 48-49 0.6788028555904972 1104 50-51 0.5821195038543081 1104 52-53 0.5019044437450049 1104 54-55 0.8040892239375879 1104 56-57 1.0399049730002474 1104 58-59 1.0572433219572588 1104 60-61 1.025468366380494 1104 62-63 1.3093266611673897 1104 64-65 1.0071067872241954 1104 66-67 0.9888654964421022 1104 68-69 1.149181707321624 1104 70-71 1.3168368968682955 1104 72-73 1.2579675707111484 1104 74-75 1.2998124210539288 1104 76-77 1.2889259997188454 1104 78-79 1.4290451845403673 1104 80-81 1.7080395728406899 1104 82-83 1.7485470157392413 1104 84-85 1.9453976277264822 1104 86-87 2.092063321504071 1104 88-89 2.161443503710906 1104 90-91 2.204772225578676 1104 92-93 2.524355491204652 1104 94-95 3.0869903327477637 1104 96-97 3.1944703372492995 1104 98-99 3.6158068130689216 1104 100-101 4.184332657018725 1105 1 0.2997121234339666 1105 2 0.24641427071397004 1105 3 0.18256898452725068 1105 4 0.05561106229587409 1105 5 0.07054407438736376 1105 6 0.14429182862144074 1105 7 0.22583085520757606 1105 8 0.23134093746288187 1105 9 0.30107188911247107 1105 10-11 0.3055538808079419 1105 12-13 0.33425897386401715 1105 14-15 0.4680940654609884 1105 16-17 0.4308711910589338 1105 18-19 0.4535281726056297 1105 20-21 0.42636449191549985 1105 22-23 0.5385470416159919 1105 24-25 0.4415272479797565 1105 26-27 0.4318729762080693 1105 28-29 0.4081446632001118 1105 30-31 0.43937414807437847 1105 32-33 0.4506010148862458 1105 34-35 0.44323528512669697 1105 36-37 0.521341398020688 1105 38-39 0.5288547186313366 1105 40-41 0.7266722643743293 1105 42-43 0.8891413214520227 1105 44-45 0.8920469076602657 1105 46-47 0.8604883528009637 1105 48-49 0.7184952539103406 1105 50-51 0.6080368449737321 1105 52-53 0.6440464288558161 1105 54-55 0.8130097153489828 1105 56-57 0.8505203471080947 1105 58-59 0.6540763820945443 1105 60-61 0.8880080651818361 1105 62-63 1.0862908980966708 1105 64-65 0.9683257538601353 1105 66-67 1.2800754134588033 1105 68-69 1.0139432583922101 1105 70-71 1.3364984752584803 1105 72-73 1.487850309843072 1105 74-75 1.214689440143502 1105 76-77 1.2271303332912247 1105 78-79 1.6077954406616648 1105 80-81 1.6787190199601767 1105 82-83 1.9786101069525728 1105 84-85 2.1753934870988125 1105 86-87 2.4711047021686205 1105 88-89 2.447333567911979 1105 90-91 2.9550678322446586 1105 92-93 3.0669356571712534 1105 94-95 3.171503958400873 1105 96-97 3.7563108249042543 1105 98-99 4.058526351709087 1105 100-101 4.569488933698029 1106 1 0.26928715576131523 1106 2 0.2749478144490425 1106 3 0.1438660235611593 1106 4 0.28649190039612193 1106 5 0.17523427974822425 1106 6 0.1776799540734757 1106 7 0.170341780756047 1106 8 0.17469684514912132 1106 9 0.24978865769247705 1106 10-11 0.09081151335008286 1106 12-13 0.1184196681897518 1106 14-15 0.32531237917874023 1106 16-17 0.06836982125596336 1106 18-19 0.18016919656074037 1106 20-21 0.04405029199001831 1106 22-23 0.23682218571926228 1106 24-25 0.23877558777856223 1106 26-27 0.3148183813409986 1106 28-29 0.038221152997820695 1106 30-31 0.32743823293641583 1106 32-33 0.52860745843941 1106 34-35 0.511763787986844 1106 36-37 0.5221249253181242 1106 38-39 0.483796967738229 1106 40-41 0.5836516844545443 1106 42-43 0.20064547469462468 1106 44-45 0.05171333324161509 1106 46-47 0.2598166014256833 1106 48-49 0.4804125397279506 1106 50-51 -0.07988587622205046 1106 52-53 0.1982533677297269 1106 54-55 0.20480295180984598 1106 56-57 0.22477133884544287 1106 58-59 -0.18007444964981545 1106 60-61 0.6895433403478606 1106 62-63 1.1140233810563203 1106 64-65 1.0705656556726524 1106 66-67 0.9979595610030145 1106 68-69 0.6229017697999311 1106 70-71 0.8137476917311872 1106 72-73 0.8852389174657525 1106 74-75 1.301957943441991 1106 76-77 1.5256016331794484 1106 78-79 1.810520800645886 1106 80-81 2.321704532923995 1106 82-83 2.1260765193838083 1106 84-85 2.0660349932245694 1106 86-87 2.427626060136493 1106 88-89 2.5247570233221523 1106 90-91 2.7905128538327517 1106 92-93 2.9557942277544633 1106 94-95 2.800522960307635 1106 96-97 2.839255134194808 1106 98-99 3.1815543833570175 1106 100-101 3.228308273124245 1107 1 0.3188206043979598 1107 2 0.3836083743106755 1107 3 0.40857466917429264 1107 4 0.2698882235016242 1107 5 0.23369092356774956 1107 6 0.33031591589526244 1107 7 0.2591509537750696 1107 8 0.2812990273710554 1107 9 0.35900865214083666 1107 10-11 0.3429830590550367 1107 12-13 0.500733421312539 1107 14-15 0.5485267792812323 1107 16-17 0.7177441940272971 1107 18-19 0.653776482021847 1107 20-21 0.5758259629912317 1107 22-23 0.5130953264533602 1107 24-25 0.5308997310108978 1107 26-27 0.6401157144181013 1107 28-29 0.6981404406795804 1107 30-31 0.7866165900777489 1107 32-33 0.6065695791662478 1107 34-35 0.7177243286312631 1107 36-37 0.7432884211437241 1107 38-39 0.9409884779980828 1107 40-41 0.9560685699839695 1107 42-43 1.015914622517741 1107 44-45 1.0697583015332057 1107 46-47 0.9202622273732004 1107 48-49 1.2109230771695039 1107 50-51 0.9686621561482838 1107 52-53 0.8706382245614535 1107 54-55 1.032410621615334 1107 56-57 1.0279859524725836 1107 58-59 1.0342765421721936 1107 60-61 1.2507320320823254 1107 62-63 1.58681713759551 1107 64-65 1.3941921583523893 1107 66-67 1.655848229249763 1107 68-69 1.784389396046386 1107 70-71 1.4491105768762793 1107 72-73 1.7648035407015037 1107 74-75 1.621834953242768 1107 76-77 1.7901341209551624 1107 78-79 1.9592887634071872 1107 80-81 2.333757999496708 1107 82-83 2.4734800596225277 1107 84-85 2.7484021275102215 1107 86-87 2.9751822705009765 1107 88-89 3.23407737435668 1107 90-91 3.3843217065648012 1107 92-93 3.5628864490514083 1107 94-95 3.7700903593638593 1107 96-97 3.8956256421699536 1107 98-99 4.3324607955023 1107 100-101 4.607548338889838 1108 1 0.293342124312165 1108 2 0.370517947095955 1108 3 0.24292773862975992 1108 4 0.24056160749346134 1108 5 0.3298167221952326 1108 6 0.26553829795905415 1108 7 0.23085700712766766 1108 8 0.18242939242664136 1108 9 0.24628254430945873 1108 10-11 0.10614063962413667 1108 12-13 0.2732865023084443 1108 14-15 0.10752025495978046 1108 16-17 0.18561703442517086 1108 18-19 0.3799688768795946 1108 20-21 0.3660179917388362 1108 22-23 0.32814305010386846 1108 24-25 0.3824878332272519 1108 26-27 0.4270316543362469 1108 28-29 0.29084191566570183 1108 30-31 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-0.3113879418514003 2316 54-55 -0.7594844819888991 2316 56-57 -0.4889022288001996 2316 58-59 -0.4308568280802447 2316 60-61 -0.5219997506487672 2316 62-63 -0.4379867296803184 2316 64-65 -0.3562277964217131 2316 66-67 -0.19112325074469538 2316 68-69 -0.13471739245331804 2316 70-71 -0.23224412338677425 2316 72-73 -0.3373296911043049 2316 74-75 -0.037485966042844154 2316 76-77 0.07933297646303217 2316 78-79 0.19714814778117784 2316 80-81 0.5338975998474425 2316 82-83 0.9649667456717239 2316 84-85 0.9594870875914445 2316 86-87 1.3069592329819422 2316 88-89 1.2907055067116602 2316 90-91 1.3575807402073323 2316 92-93 1.839171695255665 2316 94-95 1.7737776545782218 2316 96-97 1.852122250227545 2316 98-99 2.039414277435011 2316 100-101 2.0907420718724374 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3377.0 3 594.0 4 422.0 5 412.0 6 389.0 7 420.0 8 513.0 9 570.0 10 828.0 11 1245.0 12 1820.0 13 2657.0 14 3315.0 15 5079.0 16 6786.0 17 8991.0 18 7750.0 19 5155.0 20 4976.0 21 4833.0 22 5169.0 23 5878.0 24 6619.0 25 7802.0 26 8895.0 27 10740.0 28 13071.0 29 16031.0 30 19592.0 31 24542.0 32 30838.0 33 39577.0 34 51225.0 35 67776.0 36 86643.0 37 107035.0 38 78362.0 39 7866.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.45364766342121 21.076420599662555 15.389068619104377 22.080863117811866 2 15.110710993271947 22.013567289028344 40.70526431197661 22.17045740572309 3 18.613949150029647 25.679327683336627 31.592520755089936 24.114202411543783 4 11.934181163952527 18.60949601179859 38.859366675160416 30.596956149088467 5 12.315738097885854 38.324313303925564 35.616605503453556 13.743343094735023 6 29.28018163424485 37.902248032681804 18.574263848452787 14.243306484620552 7 25.330784541101593 34.45054020292545 22.570090499624012 17.64858475634895 8 23.975101953208842 35.2082532299981 21.53146767840074 19.285177138392314 9 24.731762307739825 17.22496757057261 22.739360059299525 35.30391006238804 10-11 23.09367670288641 27.775195677718912 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19.361448657479503 36-37 19.6735879385501 28.582248458579684 31.753104072314347 19.99105953055587 38-39 19.20451887380179 28.977733723338638 31.931800022696272 19.885947380163305 40-41 19.98579561902924 28.702730869439353 31.638791855640257 19.67268165589115 42-43 19.488436831992267 28.772166566437363 31.362251353481614 20.37714524808876 44-45 19.988050890239553 28.807234923511803 31.12611868522741 20.078595501021233 46-47 19.987540402983818 28.84859891291174 30.73398142794393 20.429879256160515 48-49 19.85382324469785 28.773029246158384 30.952248617820654 20.420898891323112 50-51 19.851302607376052 29.133311257204653 30.514613084969167 20.50077305045013 52-53 19.948638860329194 29.41996029430381 30.065563799273193 20.565837046093804 54-55 20.05878734126993 29.04701366327111 30.232563524832173 20.661635470626784 56-57 20.13126653760772 29.117227517344226 30.043873868032595 20.707632077015464 58-59 20.262461559362226 29.053094394150847 30.01387876841754 20.670565278069382 60-61 20.110952186508356 28.93625922703979 30.301123956301478 20.651664630150375 62-63 19.763049344522454 28.85845637045133 30.485958022267095 20.89253626275912 64-65 19.89315371754357 28.848650643069195 30.129385738082266 21.128809901304972 66-67 19.78318172520657 28.833974628634063 29.98737992597767 21.3954637201817 68-69 20.037715255226374 28.522567014821337 30.205704837602866 21.234012892349423 70-71 19.999737536252805 28.269275453386822 30.138712090392517 21.592274919967856 72-73 19.868368133848886 27.90492121926988 30.67156799551655 21.55514265136468 74-75 19.887514946416758 28.389893636319425 29.99071373047896 21.73187768678486 76-77 19.748723625320928 28.227989001641106 30.08006385347049 21.943223519567475 78-79 19.848463680143507 28.108320063356263 29.99858745178618 22.04462880471405 80-81 19.941057870734877 28.169027130669978 29.74694096833057 22.142974030264575 82-83 20.007039975792367 28.014626476021242 30.057840502864234 21.92049304532216 84-85 20.1417008736085 27.884922650372896 29.84039161866889 22.13298485734972 86-87 19.839546023615785 27.924379464237475 30.01904947150838 22.217025040638358 88-89 20.395625637850614 27.653265563555014 29.683888568944994 22.267220229649382 90-91 20.48609770747668 27.596440543708027 29.52262645010168 22.394835298713613 92-93 20.487372214935963 27.38326912076774 29.78832625592754 22.341032408368758 94-95 20.489987184831783 27.448700727222196 29.610140966850402 22.45117112109562 96-97 20.336180383147443 27.34912488729141 29.70990662170675 22.604788107854397 98-99 20.771355986762728 27.081678481249128 29.18692051229203 22.960045019696118 100-101 21.20291109119565 26.570660403597145 29.575852158595755 22.650576346611455 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1688.0 1 968.5 2 293.5 3 336.0 4 253.5 5 238.5 6 443.5 7 794.0 8 1055.0 9 1130.0 10 949.0 11 736.5 12 764.5 13 795.0 14 841.5 15 971.5 16 1073.5 17 1181.5 18 1330.5 19 1515.5 20 1803.0 21 2155.0 22 2485.5 23 2881.5 24 3535.5 25 4341.5 26 5360.0 27 6656.5 28 7861.5 29 9426.0 30 11235.0 31 13040.0 32 15053.0 33 16912.5 34 18682.0 35 20494.0 36 22411.0 37 23487.5 38 23665.0 39 23806.5 40 23967.5 41 24154.0 42 24166.5 43 24144.0 44 24071.0 45 24078.5 46 24286.0 47 24928.0 48 26111.5 49 25179.5 50 22556.5 51 20374.0 52 18004.5 53 15747.5 54 13967.0 55 12328.0 56 10609.0 57 8863.0 58 7153.0 59 5622.0 60 4218.0 61 3034.5 62 2209.0 63 1607.0 64 1136.5 65 811.0 66 579.5 67 434.0 68 358.5 69 281.5 70 217.0 71 176.5 72 135.5 73 108.5 74 96.0 75 66.5 76 49.5 77 45.5 78 34.5 79 25.5 80 17.0 81 10.0 82 6.5 83 4.5 84 2.5 85 2.5 86 1.5 87 0.5 88 1.0 89 1.0 90 1.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27230878952259513 2 0.03195460285214127 3 0.037357555025208634 4 0.03998184608069849 5 0.05341204148232308 6 0.052331451047709615 7 0.02577980036863571 8 0.02979342198291432 9 0.03612259452850752 10-11 0.02670602074116154 12-13 0.029716236951870503 14-15 0.024236099747759317 16-17 0.018678777512604317 18-19 0.022692399126882926 20-21 0.034347338814499674 22-23 0.02855846148621321 24-25 0.04785471924716808 26-27 0.04021340117382995 28-29 0.033652673535105294 30-31 0.033575488504061474 32-33 0.029098756703519947 34-35 0.028944386641432306 36-37 0.0273235009895121 38-39 0.017057891860684106 40-41 0.01644041161233355 42-43 0.017366631984859385 44-45 0.007255392918119032 46-47 0.015514191239807717 48-49 0.013121455277449314 50-51 0.005480137204111183 52-53 0.004322361738453891 54-55 0.005480137204111183 56-57 0.004322361738453891 58-59 0.006483542607680837 60-61 0.005788877328286461 62-63 0.003396141365928057 64-65 0.006329172545593198 66-67 0.0025471060244460428 68-69 0.007023837824987573 70-71 0.013198640308493133 72-73 0.012118049873879658 74-75 0.009802498942565076 76-77 0.009416573787345978 78-79 0.004399546769497711 80-81 0.0061748024835055594 82-83 0.009956869004652714 84-85 0.0032417713038404186 86-87 0.0010805904346134728 88-89 0.017135076891727927 90-91 0.02577980036863571 92-93 0.011654939687616743 94-95 0.019141887698867235 96-97 0.01636322658128973 98-99 0.01196367981179202 100-101 0.09478321812181033 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 647794.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.32741464107833 #Duplication Level Percentage of deduplicated Percentage of total 1 82.11154915338079 60.21027611591238 2 12.34808517871772 18.10906323846376 3 3.2159812289177623 7.074587671523322 4 1.1377278806482656 3.337065762120426 5 0.49174839099765283 1.8029319082884 6 0.2543167902843317 1.118903563882041 7 0.12876060733544517 0.6609177710466284 8 0.07850423384566586 0.46052100050250533 9 0.050016008663491 0.33007901453636135 >10 0.16183809899878723 2.035661909935598 >50 0.009683700475223081 0.48686737752309084 >100 0.009473084787246372 1.429408203738051 >500 0.001052564976192197 0.5324005285399698 >1k 0.0012630779714306363 2.411315933987494 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4611 0.7118003562861033 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4011 0.6191783190335199 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2678 0.4134030262706972 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1977 0.30518961274726225 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1311 0.2023791513968947 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1033 0.15946427413653105 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 906 0.1398592762514009 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 743 0.11469695613111576 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 708 0.1092940039580484 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.5437006208763896E-4 0.0 14-15 0.0 0.0 0.0 3.087401241752779E-4 0.0 16-17 0.0 0.0 0.0 4.631101862629169E-4 0.0 18-19 0.0 0.0 0.0 5.402952173067364E-4 0.0 20-21 0.0 0.0 0.0 0.0011577754656572922 0.0 22-23 0.0 0.0 0.0 0.0027014760865336817 0.0 24-25 0.0 0.0 0.0 0.003627696459059516 0.0 26-27 0.0 0.0 0.0 0.007023837824987573 0.0 28-29 0.0 0.0 0.0 0.01520545111563244 0.0 30-31 0.0 0.0 0.0 0.03905562570817266 0.0 32-33 0.0 0.0 0.0 0.09015211625918115 0.0 34-35 0.0 0.0 0.0 0.17667653605930278 0.0 36-37 0.0 0.0 0.0 0.3098978996409353 0.0 38-39 0.0 0.0 0.0 0.48302392427222235 0.0 40-41 0.0 0.0 0.0 0.650361071575223 0.0 42-43 0.0 0.0 0.0 0.8288128633485337 0.0 44-45 0.0 0.0 0.0 1.0665427589634975 0.0 46-47 0.0 0.0 0.0 1.335841332275384 0.0 48-49 0.0 0.0 0.0 1.6008947288798598 0.0 50-51 0.0 0.0 0.0 1.8647903500186787 0.0 52-53 0.0 0.0 0.0 2.1187291021528445 0.0 54-55 0.0 0.0 0.0 2.4275464113591667 0.0 56-57 0.0 0.0 0.0 2.7842184398126566 0.0 58-59 0.0 0.0 0.0 3.1631969422378106 0.0 60-61 0.0 0.0 0.0 3.5585386712442535 0.0 62-63 0.0 0.0 0.0 3.9488633732328484 0.0 64-65 0.0 0.0 0.0 4.397076848504309 0.0 66-67 0.0 0.0 0.0 4.8924503777435415 0.0 68-69 0.0 0.0 0.0 5.444940829955202 0.0 70-71 0.0 0.0 0.0 6.012327993158319 0.0 72-73 0.0 0.0 0.0 6.582956927665276 0.0 74-75 0.0 0.0 0.0 7.166784502480727 0.0 76-77 0.0 0.0 0.0 7.801477012754054 0.0 78-79 0.0 0.0 0.0 8.535197917856603 0.0 80-81 0.0 0.0 0.0 9.280650947677811 0.0 82-83 0.0 0.0 0.0 10.00850579042103 0.0 84-85 0.0 0.0 0.0 10.759284587384261 0.0 86-87 0.0 0.0 0.0 11.567874972599313 0.0 88-89 0.0 0.0 0.0 12.434817241283493 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGCGAC 30 0.0039525502 31.66435 90-91 GGTATCA 3395 0.0 29.0403 1 GGTCGCG 60 5.3467484E-6 27.732 94-95 GTATCAA 4670 0.0 26.925116 1 TCGCCGG 45 8.903671E-4 26.411427 94-95 GCCGTAT 90 6.6393113E-9 26.411427 94-95 TAGCCCT 790 0.0 23.453085 4 TATCAAC 5405 0.0 23.292313 2 TCAACGC 5415 0.0 23.249298 4 CAACGCA 5450 0.0 23.09999 5 ATCAACG 5440 0.0 23.055124 3 GTACATG 2650 0.0 22.825926 1 GTGTAGC 585 0.0 22.79674 1 AACGCAG 5545 0.0 22.70423 6 GAGTACT 3480 0.0 22.588146 12-13 GTATAGC 550 0.0 22.51548 1 ATAGCCC 380 0.0 22.503567 3 CGCCGTA 75 3.013665E-5 22.185598 94-95 TACATGG 2685 0.0 22.1171 2 GTAGCCC 520 0.0 21.926552 3 >>END_MODULE