FastQCFastQC Report
Thu 26 May 2016
SRR938317_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938317_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences647794
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43940.6783020528130856No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40600.6267424520758142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28460.43933719670142046No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19020.2936118580906893No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15700.2423609974775932No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10310.15915553401235577No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT9770.15081955065962327RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT9700.14973896022500982No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7580.11701250706243034No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7220.11145518482727533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG200.002066190671.31494
TAGGACG1303.009318E-729.2573994
GGTATCA44800.023.8814491
AGGACGT1601.8428127E-623.7716355
GTATCAA57550.023.2175921
GGACGTG1503.0327645E-522.1645956
GTGTAGC4400.021.6139161
GAGTACT40400.021.5728612-13
GTACATG25000.021.4928781
GTATAGC4500.021.1336081
AGTACTT40600.021.11563712-13
TAGCCCT6150.020.8726564
TACATGG25300.020.6741832
CTCCGAC1753.6379788E-1220.3552494-95
AGAGTAC54150.020.34897610-11
GTACTTT43100.020.22142814-15
CATGGGT7550.020.150794
ACTATAC2151.0679341E-619.9018343
ACAGTAC2151.0782696E-619.8818658
GGTGTAC1200.004736277319.8096963
Produced by FastQC (version 0.11.5)