##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938317_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 647794 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.366016048311653 31.0 31.0 34.0 30.0 34.0 2 31.629754211987144 31.0 31.0 34.0 30.0 34.0 3 31.66277551196831 31.0 31.0 34.0 30.0 34.0 4 35.430014788651945 37.0 35.0 37.0 33.0 37.0 5 35.307259715279855 37.0 35.0 37.0 33.0 37.0 6 35.358408691651974 37.0 35.0 37.0 33.0 37.0 7 35.281802548340984 37.0 35.0 37.0 32.0 37.0 8 35.27791859757886 37.0 35.0 37.0 32.0 37.0 9 36.743984970530754 39.0 35.0 39.0 32.0 39.0 10-11 36.67283426521394 39.0 35.0 39.0 32.0 39.0 12-13 36.56581258856983 38.0 35.0 39.0 32.0 39.0 14-15 37.43139099775546 39.0 36.0 40.5 32.0 41.0 16-17 37.323025529720866 39.0 36.0 40.0 32.0 41.0 18-19 37.38891530332174 39.0 36.0 40.0 32.0 41.0 20-21 37.40571462532842 39.0 36.0 40.0 32.0 41.0 22-23 37.38774749380204 39.0 36.0 40.5 32.0 41.0 24-25 37.28707428596128 39.0 36.0 41.0 31.5 41.0 26-27 37.06401649289744 39.0 36.0 40.0 31.0 41.0 28-29 36.90083421581552 39.0 36.0 40.0 31.0 41.0 30-31 36.59259965359358 38.5 35.0 40.0 30.0 41.0 32-33 36.26796018487359 38.0 35.0 40.0 30.0 41.0 34-35 35.99072930592133 38.0 35.0 40.0 30.0 41.0 36-37 35.91758413940234 38.0 35.0 40.0 30.0 41.0 38-39 35.70961216065601 38.0 35.0 40.0 29.0 41.0 40-41 35.48243577433567 38.0 34.0 40.0 28.0 41.0 42-43 35.164830949345 38.0 34.0 40.0 27.0 41.0 44-45 34.73280626248468 38.0 33.5 40.0 25.5 41.0 46-47 34.17094014455212 38.0 33.0 40.0 24.0 41.0 48-49 33.619797342982494 38.0 33.0 40.0 22.0 41.0 50-51 33.16255707833046 37.0 32.0 40.0 18.5 41.0 52-53 32.564596924330885 37.0 31.0 39.0 15.0 40.5 54-55 32.178404708904374 36.5 31.0 39.0 11.0 40.0 56-57 31.734171665683846 36.0 30.0 39.0 8.5 40.0 58-59 31.20106777771946 35.5 29.5 39.0 7.5 40.0 60-61 30.702013294349747 35.0 28.0 38.5 4.0 40.0 62-63 30.133652056054856 34.5 28.0 38.0 2.0 40.0 64-65 29.56684455243488 34.0 27.0 38.0 2.0 40.0 66-67 28.979392368561612 34.0 26.0 38.0 2.0 40.0 68-69 28.33433699602034 33.5 26.0 37.0 2.0 39.0 70-71 27.700090615226443 33.0 24.5 36.5 2.0 39.0 72-73 27.02601830211456 32.0 22.5 36.0 2.0 39.0 74-75 26.340559653223092 31.5 21.5 35.5 2.0 38.0 76-77 25.227411800665024 30.5 20.5 34.0 2.0 36.0 78-79 25.795951336381627 31.0 21.5 35.0 2.0 37.0 80-81 25.38497963858881 31.0 19.5 35.0 2.0 36.5 82-83 24.769440593769005 31.0 17.0 34.5 2.0 36.0 84-85 24.02534756419479 30.5 9.5 34.0 2.0 36.0 86-87 23.217926532200053 30.0 4.5 34.0 2.0 35.0 88-89 22.324936631089514 29.0 2.0 34.0 2.0 35.0 90-91 21.608460405622775 29.0 2.0 34.0 2.0 35.0 92-93 20.674667409701232 28.0 2.0 33.5 2.0 35.0 94-95 19.717280493490215 26.5 2.0 33.0 2.0 35.0 96-97 18.59296705434135 25.0 2.0 33.0 2.0 34.0 98-99 17.32713331707302 22.0 2.0 32.0 2.0 34.0 100-101 14.681704368981498 2.0 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3604687778333471 1101 2 0.4418826009149619 1101 3 0.47401075708076235 1101 4 0.3240503246925215 1101 5 0.33336235483347565 1101 6 0.30516033822797795 1101 7 0.36793773037868505 1101 8 0.13554129586266583 1101 9 0.3194537143160403 1101 10-11 0.287121893961384 1101 12-13 0.17629327629817482 1101 14-15 0.5832496446168633 1101 16-17 0.5973172672132776 1101 18-19 0.7192807975387225 1101 20-21 0.5303752728923854 1101 22-23 0.6941295424095841 1101 24-25 0.6339328655267664 1101 26-27 0.6218858763377924 1101 28-29 0.6548656936253323 1101 30-31 0.7521810816677217 1101 32-33 0.5697004892707938 1101 34-35 0.4828708619918842 1101 36-37 0.4990195759575471 1101 38-39 0.3700846282782919 1101 40-41 0.5258277890043601 1101 42-43 0.45428380124837275 1101 44-45 0.188938785991418 1101 46-47 0.31836458227164144 1101 48-49 0.5062858734252629 1101 50-51 0.6017884530664261 1101 52-53 1.0241153957869997 1101 54-55 0.9210780570302077 1101 56-57 1.2324963703806304 1101 58-59 1.340224494621399 1101 60-61 1.4846392684310494 1101 62-63 1.4485332247202898 1101 64-65 1.8615965188650456 1101 66-67 1.895295450092167 1101 68-69 1.5539777074743917 1101 70-71 1.8594805061098647 1101 72-73 2.2932730906387526 1101 74-75 2.077085881312321 1101 76-77 2.182871611241218 1101 78-79 2.87472524115838 1101 80-81 2.987465715757569 1101 82-83 2.9869813744726024 1101 84-85 3.2974370137659896 1101 86-87 3.2378524039440784 1101 88-89 3.6237019615348594 1101 90-91 4.336749271079082 1101 92-93 4.6194451037031286 1101 94-95 4.49117028871899 1101 96-97 4.5403132366579335 1101 98-99 4.963795314097823 1101 100-101 4.7492435870998 1102 1 0.47815470564960805 1102 2 0.37766611045439547 1102 3 0.41515640053683 1102 4 0.3970695250813847 1102 5 0.452096405143358 1102 6 0.3905139656432155 1102 7 0.5437944565908595 1102 8 0.5722058359039863 1102 9 0.652240201130958 1102 10-11 0.5610160487797131 1102 12-13 0.6626752789716548 1102 14-15 0.6912594270934633 1102 16-17 0.8300194485232808 1102 18-19 0.8680040628200416 1102 20-21 0.9335340710452655 1102 22-23 0.9887393066579762 1102 24-25 0.9348161745558059 1102 26-27 0.7854613411573297 1102 28-29 0.8444771287936419 1102 30-31 1.0199991640592643 1102 32-33 0.8028773637391353 1102 34-35 1.0181386648840984 1102 36-37 1.1386059473270933 1102 38-39 1.0783799861589571 1102 40-41 1.2042630484526526 1102 42-43 1.515363519318619 1102 44-45 1.4990387373828327 1102 46-47 1.2293918942171942 1102 48-49 1.5246545998804848 1102 50-51 1.7385380581217262 1102 52-53 1.7576530370556895 1102 54-55 1.6935025618303072 1102 56-57 2.2012045973826986 1102 58-59 2.080236950569873 1102 60-61 2.090161658757946 1102 62-63 2.2648003896248987 1102 64-65 2.3843590051938826 1102 66-67 2.2552002076568733 1102 68-69 2.4236573465556894 1102 70-71 2.466632043356192 1102 72-73 2.5434022071778237 1102 74-75 2.4718862823331023 1102 76-77 2.459416452655727 1102 78-79 3.2304830792918082 1102 80-81 3.5678407613281102 1102 82-83 3.4762229287319286 1102 84-85 3.721740543964067 1102 86-87 3.78437233346164 1102 88-89 4.113725810119135 1102 90-91 4.222257078832154 1102 92-93 4.396783632078385 1102 94-95 4.643630338542781 1102 96-97 5.033656139805341 1102 98-99 5.0555060322895855 1102 100-101 4.720272114259844 1103 1 0.3090086638732359 1103 2 0.38056968543537906 1103 3 0.4580444702811377 1103 4 0.28226494957380766 1103 5 0.082472041443161 1103 6 0.20342719482816563 1103 7 0.2751077208820121 1103 8 0.27971271086741467 1103 9 0.3596958795582026 1103 10-11 0.5486959680354602 1103 12-13 0.503216353221255 1103 14-15 0.740894468928353 1103 16-17 0.7263830316957112 1103 18-19 0.6038665050410899 1103 20-21 0.5278527182865034 1103 22-23 0.5485039488673209 1103 24-25 0.6386515264954298 1103 26-27 0.5757676427195548 1103 28-29 0.5826909547839279 1103 30-31 0.6805405308605046 1103 32-33 0.7815357219393562 1103 34-35 1.0551939863983435 1103 36-37 1.0133536215415901 1103 38-39 1.0348519597122845 1103 40-41 0.8388916541515599 1103 42-43 1.0125696512008915 1103 44-45 1.0888082066941678 1103 46-47 1.211853423677148 1103 48-49 1.351460374849772 1103 50-51 1.1997822802269198 1103 52-53 1.5198122144004884 1103 54-55 1.688854647723467 1103 56-57 1.4807157436000082 1103 58-59 2.015082044478948 1103 60-61 2.063016523891637 1103 62-63 2.4681201193071836 1103 64-65 2.17166299559819 1103 66-67 2.295016485229869 1103 68-69 2.510025903105923 1103 70-71 2.7492003541630474 1103 72-73 2.7244221884545183 1103 74-75 2.640238777798551 1103 76-77 2.6401365685061755 1103 78-79 3.0738289923454616 1103 80-81 3.3827945381697226 1103 82-83 3.864563283304509 1103 84-85 3.9742235759692157 1103 86-87 4.005124483716163 1103 88-89 4.355687953937519 1103 90-91 4.5580515027979835 1103 92-93 4.6518228339975245 1103 94-95 4.998916015214718 1103 96-97 5.467930758025457 1103 98-99 5.808405950375125 1103 100-101 5.125935182541392 1104 1 0.26561934432112366 1104 2 0.21860442560189952 1104 3 0.1252853545762882 1104 4 0.1900312429990194 1104 5 0.26717273050432055 1104 6 0.256524381161789 1104 7 0.2670507297823832 1104 8 0.3266191133939742 1104 9 0.22575961771496367 1104 10-11 0.30625949086061155 1104 12-13 0.2561233299654404 1104 14-15 0.466355637461227 1104 16-17 0.4486142260683792 1104 18-19 0.4887538148315045 1104 20-21 0.5354252132621014 1104 22-23 0.3443606097918206 1104 24-25 0.47524175044029704 1104 26-27 0.34883504151370204 1104 28-29 0.5700043340546728 1104 30-31 0.6713261323534709 1104 32-33 0.5084862674461093 1104 34-35 0.5545390194158557 1104 36-37 0.8799826051763873 1104 38-39 0.9513122669187197 1104 40-41 0.8232711402267796 1104 42-43 1.156393222834545 1104 44-45 1.291614695353367 1104 46-47 1.416158261460673 1104 48-49 1.4965400475457145 1104 50-51 1.2997051197330975 1104 52-53 1.3325903510642902 1104 54-55 1.4756584088489362 1104 56-57 1.8929950286990618 1104 58-59 2.010263999689979 1104 60-61 2.0971914449367155 1104 62-63 2.102379378566443 1104 64-65 1.9821801503498406 1104 66-67 2.227249832918641 1104 68-69 2.2061840282853638 1104 70-71 2.4696102019953763 1104 72-73 2.4895312898039315 1104 74-75 2.5414195127971198 1104 76-77 2.277329720960953 1104 78-79 2.5552745852251633 1104 80-81 2.699376866622206 1104 82-83 3.2226328354147604 1104 84-85 3.547176131237663 1104 86-87 3.7192179376351966 1104 88-89 3.9447365471043305 1104 90-91 3.980271930590117 1104 92-93 4.182839558085956 1104 94-95 4.38853164828965 1104 96-97 4.357788639162408 1104 98-99 4.5730305556005035 1104 100-101 4.202377904332954 1105 1 0.38073733086621786 1105 2 0.36829878751836986 1105 3 0.30668753331480403 1105 4 0.2624751524345257 1105 5 0.29959555261953597 1105 6 0.37297633280608267 1105 7 0.3899782816042574 1105 8 0.2827583409321335 1105 9 0.37790847541397454 1105 10-11 0.5681424519643201 1105 12-13 0.43851371004944895 1105 14-15 0.748709093391291 1105 16-17 0.7604152283563934 1105 18-19 0.7340603920264641 1105 20-21 0.5824021281956817 1105 22-23 0.3251974007626899 1105 24-25 0.38808047085829145 1105 26-27 0.6031987081462162 1105 28-29 0.6320838682553998 1105 30-31 0.6327724066423102 1105 32-33 0.6587451633925596 1105 34-35 0.7964615939471571 1105 36-37 1.071611493951444 1105 38-39 1.0160309176717135 1105 40-41 1.3586916487129272 1105 42-43 1.0874731156050927 1105 44-45 1.0524102081082063 1105 46-47 1.3963995704112548 1105 48-49 1.3909753854550857 1105 50-51 1.4557715661354464 1105 52-53 1.379111583519979 1105 54-55 1.6714204550873646 1105 56-57 1.7374664370247075 1105 58-59 1.9019620412157785 1105 60-61 1.7854800851033623 1105 62-63 2.328976294865999 1105 64-65 2.4309868385306963 1105 66-67 2.705659874581041 1105 68-69 2.9321651862951335 1105 70-71 2.7462702948820343 1105 72-73 3.0614095980373754 1105 74-75 2.927680312578559 1105 76-77 2.6170394074684857 1105 78-79 2.9683128668219965 1105 80-81 3.2300847432058895 1105 82-83 3.512731826296843 1105 84-85 3.73972705668929 1105 86-87 3.9703512743834786 1105 88-89 4.302613771090943 1105 90-91 4.196270760969515 1105 92-93 4.3441394163655005 1105 94-95 4.593293156673354 1105 96-97 4.624716122685275 1105 98-99 4.970650334994694 1105 100-101 5.065474666959732 1106 1 0.13472729295007113 1106 2 0.0861851267473348 1106 3 -0.0012332153647065525 1106 4 -0.0067667438111200795 1106 5 0.05148807239949349 1106 6 0.09393760573658483 1106 7 0.11115433964007337 1106 8 -0.09633298657131917 1106 9 0.07502202660077018 1106 10-11 -0.04956964832717148 1106 12-13 0.036985009937673397 1106 14-15 -0.11923313379875111 1106 16-17 -0.19508370867649916 1106 18-19 -0.08502699044444029 1106 20-21 0.2461593333592873 1106 22-23 0.08574597945512608 1106 24-25 0.030801885809964347 1106 26-27 0.08316123033701928 1106 28-29 0.27735421256907244 1106 30-31 0.30488869743503955 1106 32-33 0.23191346286436243 1106 34-35 -0.04525055017803936 1106 36-37 0.0331781102093629 1106 38-39 -0.07516508469529981 1106 40-41 0.18812926240657646 1106 42-43 0.39939478479220014 1106 44-45 -0.12814406896457342 1106 46-47 -0.04369291868511738 1106 48-49 0.4675397004440214 1106 50-51 0.30185064212115975 1106 52-53 0.31676034412225107 1106 54-55 0.413856951021792 1106 56-57 0.4407627308858011 1106 58-59 0.9404206293324613 1106 60-61 0.9411236733296313 1106 62-63 1.3096554979694268 1106 64-65 0.670767012550467 1106 66-67 0.8643181251782721 1106 68-69 1.0177143129087582 1106 70-71 1.3089064532970092 1106 72-73 1.6363509704703638 1106 74-75 2.1810324944007284 1106 76-77 1.9588140146089934 1106 78-79 2.1035737868912534 1106 80-81 2.352450886059902 1106 82-83 2.773173012436626 1106 84-85 2.617813496735753 1106 86-87 2.9875318911235667 1106 88-89 3.351316293720089 1106 90-91 2.9743473384346153 1106 92-93 2.7642283987662744 1106 94-95 3.4096332623125605 1106 96-97 3.658001238954146 1106 98-99 4.23700139905764 1106 100-101 3.8879514898935223 1107 1 0.22426458503044344 1107 2 0.38121213761574424 1107 3 0.26734876742520797 1107 4 0.13879941680212227 1107 5 0.08918798485438373 1107 6 0.19391625327144624 1107 7 0.19432432661506738 1107 8 0.15138123203522014 1107 9 0.208030802463945 1107 10-11 0.1778586607711219 1107 12-13 0.4371398153099548 1107 14-15 0.7405003874362777 1107 16-17 0.6447717351344835 1107 18-19 0.5817250860827912 1107 20-21 0.5945087878038038 1107 22-23 0.4326605482420973 1107 24-25 0.27483814229577774 1107 26-27 0.43713705934648317 1107 28-29 0.24718851743957515 1107 30-31 0.33621596666827 1107 32-33 0.46432330208030237 1107 34-35 0.5353806286915699 1107 36-37 0.5257026121631085 1107 38-39 0.9016888430906604 1107 40-41 0.8213633067769948 1107 42-43 0.9400189173482971 1107 44-45 0.9616291678556124 1107 46-47 0.8662936292618468 1107 48-49 0.8220632201843756 1107 50-51 1.0548463929805934 1107 52-53 1.3551755641594028 1107 54-55 1.1512248326437273 1107 56-57 1.172795699091978 1107 58-59 1.3973046640942748 1107 60-61 1.340810859715301 1107 62-63 1.7460667891529944 1107 64-65 2.1564064121191464 1107 66-67 2.2887111813566072 1107 68-69 2.367368402973458 1107 70-71 2.2372454450336043 1107 72-73 2.4159689748689708 1107 74-75 2.5714665900170672 1107 76-77 2.604579820771317 1107 78-79 2.8048004860136686 1107 80-81 2.8179068679530097 1107 82-83 3.174986904350451 1107 84-85 3.2722121045550274 1107 86-87 3.7131399025684892 1107 88-89 3.7570483629874865 1107 90-91 4.0247135332117985 1107 92-93 4.414334093468728 1107 94-95 4.437608194518482 1107 96-97 4.496036385111772 1107 98-99 4.566476341939797 1107 100-101 4.138621957298982 1108 1 0.15648844546289098 1108 2 0.22512187554853114 1108 3 0.26226500978216194 1108 4 0.19115117701590378 1108 5 0.1349497625578806 1108 6 -0.018940977702271766 1108 7 -0.02842384731112446 1108 8 0.1551398301517466 1108 9 0.26291150826062193 1108 10-11 0.27663278872262964 1108 12-13 0.36074416963293743 1108 14-15 0.2336388069149251 1108 16-17 0.12436805891115199 1108 18-19 0.4306041722400451 1108 20-21 0.42520672505172996 1108 22-23 0.6268073623874812 1108 24-25 0.6473183992095883 1108 26-27 0.4829754416171994 1108 28-29 0.4441229419403001 1108 30-31 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0.5302436468462339 2311 66-67 0.533196441022632 2311 68-69 0.5293059182010751 2311 70-71 0.7077897659911798 2311 72-73 1.0746735497138857 2311 74-75 1.0295758040533372 2311 76-77 1.0480392856337808 2311 78-79 1.2064035246652658 2311 80-81 1.307177254219102 2311 82-83 0.9008523015804286 2311 84-85 0.966416551331772 2311 86-87 1.5269787615748065 2311 88-89 1.4822109470907883 2311 90-91 1.3734087996873008 2311 92-93 1.2888585232530083 2311 94-95 1.729235035107969 2311 96-97 1.5818014518634023 2311 98-99 1.915571430994028 2311 100-101 1.643897741169889 2312 1 -0.07215508455855613 2312 2 -0.16484250387715704 2312 3 -0.16467594281727926 2312 4 -0.18909589335060417 2312 5 -0.23762014205107818 2312 6 -0.15506114132340088 2312 7 0.0045687110818306564 2312 8 -0.24876962517505774 2312 9 -0.16967056346803133 2312 10-11 -0.23969450270037385 2312 12-13 -0.34057967034281234 2312 14-15 -0.41865502288059275 2312 16-17 -0.2872308521930904 2312 18-19 -0.20817780485544546 2312 20-21 -0.14300493367080946 2312 22-23 -0.2243226025245164 2312 24-25 -0.18880442724918822 2312 26-27 -0.29991418785918 2312 28-29 -0.5541308510670859 2312 30-31 -0.2635141337697604 2312 32-33 -0.1677315374653645 2312 34-35 -0.1349742439331152 2312 36-37 -0.15673242547745048 2312 38-39 -0.12250203763311873 2312 40-41 -0.13442036061234575 2312 42-43 -0.05839982097133145 2312 44-45 0.03186626229517486 2312 46-47 -0.05261956674085866 2312 48-49 0.06396992047786654 2312 50-51 -0.12412588173965844 2312 52-53 -0.29856289241963196 2312 54-55 -0.09791079346637588 2312 56-57 -0.07644211381558108 2312 58-59 0.33442080518369366 2312 60-61 0.2882331187388196 2312 62-63 0.8353506598624669 2312 64-65 0.5339866287551551 2312 66-67 0.4182187252705951 2312 68-69 0.9379405015531646 2312 70-71 1.336567851753916 2312 72-73 1.2788311322534973 2312 74-75 1.3680326922333634 2312 76-77 1.411051721508958 2312 78-79 1.66931027382331 2312 80-81 1.7141482900561194 2312 82-83 1.7144310081722374 2312 84-85 2.0422623130371633 2312 86-87 2.1505657644667195 2312 88-89 2.0621212527247685 2312 90-91 2.1139468372586094 2312 92-93 2.5562051864039326 2312 94-95 2.385687820156626 2312 96-97 2.0228419836841063 2312 98-99 2.2503946683518166 2312 100-101 2.292830262451938 2313 1 -0.2815240494568343 2313 2 -0.2146674386196601 2313 3 -0.2821524079480433 2313 4 -0.3293016463907321 2313 5 -0.7062781625052779 2313 6 -0.26579018701769996 2313 7 -0.1592140825660806 2313 8 -0.46614033748195993 2313 9 -0.6852339621778043 2313 10-11 -0.44433919267591193 2313 12-13 -0.4506199169696359 2313 14-15 -0.5802189535984326 2313 16-17 -0.3555906453587312 2313 18-19 -0.415719343656356 2313 20-21 -0.4741323798806789 2313 22-23 -0.41562491935478363 2313 24-25 -0.48563465395815086 2313 26-27 -0.434246225774487 2313 28-29 -0.38627149150965323 2313 30-31 -0.2791218664854469 2313 32-33 -0.4506527808071539 2313 34-35 -0.4362843678736965 2313 36-37 -0.3307379920984843 2313 38-39 -0.6954228268216767 2313 40-41 -0.504744474635082 2313 42-43 -0.5975489108266245 2313 44-45 -0.09883795635581549 2313 46-47 -0.4648339097649554 2313 48-49 -0.23824634729087535 2313 50-51 -0.03762050434913533 2313 52-53 -0.06215126523186143 2313 54-55 -0.09803622626358788 2313 56-57 -0.0048243118739677016 2313 58-59 0.13177114547422164 2313 60-61 0.6180792040460936 2313 62-63 0.529416823500366 2313 64-65 0.6042614347127824 2313 66-67 0.5881855720847078 2313 68-69 1.356541104063119 2313 70-71 1.0832747001683778 2313 72-73 1.195770831517553 2313 74-75 1.1053601951042609 2313 76-77 1.0693154720793494 2313 78-79 1.5990030283491627 2313 80-81 1.7858850368916457 2313 82-83 1.8093544401000692 2313 84-85 1.7044847659403892 2313 86-87 1.750821171593465 2313 88-89 1.9707488665388126 2313 90-91 2.169104828517238 2313 92-93 2.747957331196634 2313 94-95 2.676259382811388 2313 96-97 2.5601052563122906 2313 98-99 2.5895930662722293 2313 100-101 2.8679709958877826 2314 1 -0.3021703303702772 2314 2 -0.42495419132505674 2314 3 -0.46599883010624765 2314 4 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64-65 0.37824720688670155 2314 66-67 0.48694472823337875 2314 68-69 0.22657762100172718 2314 70-71 0.6173758365858433 2314 72-73 0.9462122214004651 2314 74-75 1.1098656015920412 2314 76-77 0.8819702276785435 2314 78-79 0.7795465623103439 2314 80-81 1.361185074355955 2314 82-83 1.722076588398462 2314 84-85 1.8867787875835695 2314 86-87 2.071989369129433 2314 88-89 2.1653662784007928 2314 90-91 2.0037472596496535 2314 92-93 2.259100810899156 2314 94-95 2.904483789734101 2314 96-97 3.125942325311218 2314 98-99 3.3200298054129576 2314 100-101 3.469159088265302 2315 1 -0.3816617009774568 2315 2 -0.4409630370536277 2315 3 -0.4716716342983531 2315 4 -0.3501682163293012 2315 5 -0.41105613651124173 2315 6 -0.5848287710234246 2315 7 -0.6345604544965511 2315 8 -0.5213213087458399 2315 9 -0.681750000002431 2315 10-11 -0.6339536152288758 2315 12-13 -0.521280666152812 2315 14-15 -0.9617248481035361 2315 16-17 -0.5566614385072342 2315 18-19 -0.5914219901107955 2315 20-21 -0.48057398959375774 2315 22-23 -0.6339984429929899 2315 24-25 -0.6112104815633046 2315 26-27 -0.8991385227603814 2315 28-29 -0.8950382958278382 2315 30-31 -0.7105849774098161 2315 32-33 -0.7375117656873655 2315 34-35 -0.7754082564306799 2315 36-37 -0.8139736125479331 2315 38-39 -0.8220009500777792 2315 40-41 -0.8121209767108084 2315 42-43 -0.8279346633471434 2315 44-45 -0.8227711570709886 2315 46-47 -1.2294126063866955 2315 48-49 -1.038508300979565 2315 50-51 -0.9604010650657102 2315 52-53 -0.8283472030895283 2315 54-55 -1.1622301989214883 2315 56-57 -0.6503421026217708 2315 58-59 -0.5418568802878418 2315 60-61 -0.2251807953849152 2315 62-63 -0.23266160504011424 2315 64-65 -0.14623478917117794 2315 66-67 -0.45210868266032733 2315 68-69 -0.06692990267502097 2315 70-71 -0.13441494270361432 2315 72-73 -0.20290320574246223 2315 74-75 0.477600325884147 2315 76-77 0.6679660932619278 2315 78-79 0.903314650383038 2315 80-81 1.0086178900859686 2315 82-83 1.1070220237140695 2315 84-85 1.6482325638443243 2315 86-87 1.781570639260675 2315 88-89 1.8265003584930923 2315 90-91 2.054706348155012 2315 92-93 2.4648000561277534 2315 94-95 2.5495970054448875 2315 96-97 3.6510012167106716 2315 98-99 3.763097446391761 2315 100-101 4.029516763377073 2316 1 -0.6009973098424162 2316 2 -1.0657166546681651 2316 3 -0.712186513390705 2316 4 -0.5662515777061614 2316 5 -0.2898696550920832 2316 6 -0.6986237808787266 2316 7 -0.4860531711928573 2316 8 -0.49252942374030795 2316 9 -0.757717501564855 2316 10-11 -0.6099211167912983 2316 12-13 -0.577025582262614 2316 14-15 -0.8731089161771806 2316 16-17 -0.858901715416998 2316 18-19 -0.6784911214025513 2316 20-21 -0.6169003681332512 2316 22-23 -0.7240518058120813 2316 24-25 -1.0159482345559496 2316 26-27 -0.9052956786663486 2316 28-29 -0.7399544056302503 2316 30-31 -0.8279471234604827 2316 32-33 -1.2782213469574941 2316 34-35 -1.0826843489262288 2316 36-37 -1.0604665696173114 2316 38-39 -0.9883210789177355 2316 40-41 -1.1459102176126805 2316 42-43 -1.0822133037971184 2316 44-45 -0.8624363173998191 2316 46-47 -1.2011194954785651 2316 48-49 -0.764862803166956 2316 50-51 -0.8037222996217466 2316 52-53 -0.6221780285306124 2316 54-55 -0.5140077576927027 2316 56-57 -0.837354746244678 2316 58-59 -0.5669818742785147 2316 60-61 -0.08143289864917946 2316 62-63 -0.5230238472400082 2316 64-65 -0.3971193620282527 2316 66-67 -0.2943602571038646 2316 68-69 0.10047791425685659 2316 70-71 0.35799167236296725 2316 72-73 0.40647615101923407 2316 74-75 0.5111119359028926 2316 76-77 0.6224394358896852 2316 78-79 0.470015770521492 2316 80-81 0.705026716584694 2316 82-83 0.9511821602459669 2316 84-85 0.9064268814252081 2316 86-87 1.086725439510662 2316 88-89 1.357081820922783 2316 90-91 1.6113718930807401 2316 92-93 1.5728104883185132 2316 94-95 2.561595226684247 2316 96-97 2.4964701845487554 2316 98-99 3.2845459344452195 2316 100-101 3.0322822554207267 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 16.0 5 37.0 6 90.0 7 229.0 8 467.0 9 877.0 10 1416.0 11 2133.0 12 3178.0 13 4352.0 14 5755.0 15 7760.0 16 9784.0 17 12222.0 18 11062.0 19 9275.0 20 9029.0 21 9540.0 22 10437.0 23 11987.0 24 13101.0 25 15006.0 26 17194.0 27 19693.0 28 23300.0 29 27070.0 30 30766.0 31 35217.0 32 40017.0 33 45826.0 34 52871.0 35 60668.0 36 66197.0 37 64374.0 38 26148.0 39 691.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.704134339002835 22.763872465629508 16.35288378712986 21.179109408237803 2 15.833177615556746 23.66869022560803 38.88059252029608 21.617539638539146 3 18.903598685997974 26.808450860770122 32.25881147034851 22.029138982883396 4 12.860878612645376 20.22263250354279 37.69037687906958 29.226112004742248 5 12.169553327256153 39.22114240687236 35.324845881680396 13.284458384191092 6 28.732745286310152 39.16862459362081 17.826191659694285 14.272438460374747 7 24.96843132230308 35.06716641401433 22.25090074931228 17.713501514370307 8 24.124953303056216 36.14821995881407 20.97410596578542 18.752720772344293 9 24.449284803502348 17.361537772810493 22.25151822956063 35.937659194126525 10-11 23.16068071022578 28.38031843456407 28.702797494265152 19.75620336094499 12-13 24.560431248205447 26.4049219350596 28.527896213919856 20.506750602815092 14-15 21.209829050593243 28.356313889909444 28.03252268468062 22.401334374816688 16-17 19.50396267949379 30.800069157787814 29.19670450791455 20.499263654803844 18-19 18.941824098401653 29.413594445147684 32.0578764236779 19.586705032772763 20-21 20.580693862555073 28.105616909079124 32.15235090167553 19.161338326690274 22-23 19.866269215213478 28.706502375755257 31.749290669564704 19.677937739466557 24-25 19.65721651473869 28.584020932871248 32.37277800504797 19.385984547342098 26-27 19.566539759586735 28.522057438576663 32.554522184036884 19.35688061779972 28-29 19.048570996335254 29.145144907177283 32.37325446052294 19.43302963596452 30-31 19.66265510331988 28.656795215763037 32.34315229841586 19.33739738250123 32-33 19.40156578399961 28.790326502163893 32.23480430787423 19.573303405962267 34-35 19.922618914364364 28.943335955417872 32.19119550843866 18.9428496217791 36-37 19.730105446133397 28.579367878951146 32.02525890688306 19.6652677680324 38-39 19.355294400993017 28.90931603373052 32.23018517203368 19.50520439324278 40-41 20.055870277213216 28.676396235896384 31.88627411325811 19.381459373632286 42-43 19.70816216575043 28.693398913481445 31.59448682144693 20.003952099321197 44-45 20.10850981551479 28.725229651322103 31.325281675518756 19.84097885764435 46-47 20.329658030928112 28.82836710829471 30.756144907512823 20.085829953264355 48-49 20.12777210038994 28.73768512829696 30.888986313550294 20.245556457762806 50-51 20.069034291765593 29.048972358496684 30.564886368197296 20.317106981540427 52-53 20.233824333044147 29.44053202098198 30.028296032380663 20.29734761359321 54-55 20.304309938275082 29.055864253037427 29.96209440199693 20.677731406690558 56-57 20.56124323473203 29.261925859146583 29.572055313880647 20.604775592240742 58-59 20.409574648730924 29.41899739732075 29.71577384168424 20.455654112264085 60-61 20.360176228862876 29.457203987687443 29.648236939520896 20.534382843928782 62-63 19.986832233703925 29.40417787136034 29.8043050722915 20.804684822644234 64-65 19.966378200477312 29.4433878671306 29.517176756808496 21.073057175583596 66-67 20.02619670063593 29.237648224776024 29.359292303156188 21.376862771431856 68-69 20.07076361879342 28.997183502999814 29.448332446987102 21.48372043121966 70-71 20.059386162885115 28.772727132390852 29.45450251160091 21.713384193123122 72-73 19.86103596284927 28.37509175377647 29.884832126287158 21.879040157087097 74-75 19.954105780541344 28.743319635563157 29.092041605819137 22.210532978076365 76-77 19.453483669191133 28.417598804558242 29.81094298496127 22.31797454128936 78-79 19.417592629755756 28.347514796370447 29.716931617149893 22.517960956723897 80-81 19.396072976959477 28.325461740508356 29.631819399237564 22.6466458832946 82-83 19.349291278292522 28.16817590994557 29.81655978980713 22.66597302195478 84-85 19.287550269001876 28.102707000223848 29.676657429777777 22.933085300996503 86-87 19.087891815957455 28.078374769406516 29.70507189885533 23.1286615157807 88-89 19.254298826940087 27.99775545583234 29.215505570452287 23.532440146775283 90-91 19.189501479268248 27.63642033293377 29.522149658876806 23.65192852892118 92-93 19.06218498810577 27.563974911738782 29.655769667162197 23.718070432993255 94-95 18.86016233555729 27.634093554432425 29.553299351337 23.952444758673284 96-97 18.579980672868228 27.504113962154637 29.59760355915615 24.318301805820987 98-99 18.7932429136423 27.337162740006853 29.251583064986708 24.61801128136414 100-101 18.60531531920937 26.995560275892732 29.643262919350157 24.755861485547744 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1626.0 1 953.5 2 301.0 3 301.5 4 228.0 5 217.0 6 394.0 7 749.0 8 1010.0 9 1041.0 10 860.5 11 669.5 12 649.5 13 682.0 14 744.5 15 832.0 16 937.5 17 1058.0 18 1225.0 19 1438.5 20 1756.5 21 2145.0 22 2531.0 23 2956.0 24 3557.0 25 4393.5 26 5501.0 27 6738.0 28 7985.5 29 9670.0 30 11460.5 31 13165.0 32 15102.5 33 17060.5 34 19103.0 35 20753.5 36 22403.5 37 23687.0 38 24031.5 39 24239.5 40 24287.0 41 24464.0 42 24651.0 43 24699.5 44 24785.5 45 24484.5 46 24432.5 47 25371.5 48 26239.0 49 25080.5 50 22681.5 51 20581.0 52 18039.0 53 15411.5 54 13473.0 55 11811.5 56 10034.5 57 8267.0 58 6600.5 59 5161.0 60 3926.5 61 2912.0 62 2099.5 63 1462.5 64 1040.0 65 696.0 66 479.5 67 354.5 68 248.0 69 181.0 70 128.0 71 100.5 72 78.5 73 50.0 74 34.5 75 30.0 76 23.0 77 16.0 78 13.0 79 7.5 80 4.5 81 2.5 82 2.0 83 1.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.03936436583234794 3 3.087401241752779E-4 4 0.0 5 0.08706471501742838 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013893305587887508 26-27 0.011732124718660562 28-29 0.0 30-31 0.0 32-33 5.402952173067364E-4 34-35 0.013970490618931327 36-37 0.0034733263969718773 38-39 0.011114644470310006 40-41 0.006715097700812296 42-43 0.005711692297242642 44-45 0.002855846148621321 46-47 2.3155509313145845E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 7.718503104381948E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.8592515521909747E-4 68-69 5.402952173067364E-4 70-71 0.0 72-73 7.718503104381948E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.718503104381948E-5 82-83 0.0012349604967011117 84-85 0.00447673180054153 86-87 0.0013893305587887508 88-89 1.5437006208763896E-4 90-91 0.0011577754656572922 92-93 1.5437006208763896E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 9.262203725258338E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 647794.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.2632393342983 #Duplication Level Percentage of deduplicated Percentage of total 1 82.50113184614524 60.4430016779464 2 12.055653510534931 17.664724569473883 3 3.1712958973751113 6.970182309878132 4 1.15536792093201 3.385839860416499 5 0.48648787352853506 1.7820838755777453 6 0.21377284078737357 0.9397014478666917 7 0.11202211265152492 0.5744971994945678 8 0.0746457628523743 0.4375032311315817 9 0.04868668356313122 0.3210249735251037 >10 0.15627072333402908 1.9896501524304777 >50 0.011594639978817576 0.586435728549767 >100 0.010329664960274232 1.6836143247766777 >500 0.0014756664228270581 0.7810327162824049 >1k 0.0012648569338517641 2.4407079326499597 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4394 0.6783020528130856 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4060 0.6267424520758142 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2846 0.43933719670142046 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1902 0.2936118580906893 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1570 0.2423609974775932 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1031 0.15915553401235577 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 977 0.15081955065962327 RNA PCR Primer, Index 39 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 970 0.14973896022500982 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 758 0.11701250706243034 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 722 0.11145518482727533 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 7.718503104381948E-5 0.0 16-17 0.0 0.0 0.0 1.5437006208763896E-4 0.0 18-19 0.0 0.0 0.0 2.3155509313145842E-4 0.0 20-21 0.0 0.0 0.0 8.490353414820143E-4 0.0 22-23 0.0 0.0 0.0 0.002392735962358404 0.0 24-25 0.0 0.0 0.0 0.003318956334884238 0.0 26-27 0.0 0.0 0.0 0.006637912669768476 0.0 28-29 0.0 0.0 0.0 0.014587970867281884 0.0 30-31 0.0 0.0 0.0 0.03805222030460301 0.0 32-33 0.0 0.0 0.0 0.08791375035891039 0.0 34-35 0.0 0.0 0.0 0.17250854438293656 0.0 36-37 0.0 0.0 0.0 0.3031828019401229 0.0 38-39 0.0 0.0 0.0 0.4755369762609719 0.0 40-41 0.0 0.0 0.0 0.6405585726326579 0.0 42-43 0.0 0.0 0.0 0.8172351086919607 0.0 44-45 0.0 0.0 0.0 1.0517232330030843 0.0 46-47 0.0 0.0 0.0 1.316081964328166 0.0 48-49 0.0 0.0 0.0 1.5754236686353995 0.0 50-51 0.0 0.0 0.0 1.8332216723217565 0.0 52-53 0.0 0.0 0.0 2.0790559961963213 0.0 54-55 0.0 0.0 0.0 2.381081022670787 0.0 56-57 0.0 0.0 0.0 2.728413662367975 0.0 58-59 0.0 0.0 0.0 3.0951197448571612 0.0 60-61 0.0 0.0 0.0 3.4765681682757172 0.0 62-63 0.0 0.0 0.0 3.8529223796453813 0.0 64-65 0.0 0.0 0.0 4.280836191752316 0.0 66-67 0.0 7.718503104381948E-5 0.0 4.754134802113017 0.0 68-69 0.0 1.5437006208763896E-4 0.0 5.281077009049174 0.0 70-71 0.0 1.5437006208763896E-4 0.0 5.825154292877056 0.0 72-73 0.0 1.5437006208763896E-4 0.0 6.369231576704941 0.0 74-75 0.0 1.5437006208763896E-4 0.0 6.929980827238289 0.0 76-77 0.0 1.5437006208763896E-4 0.0 7.5452998947196175 0.0 78-79 0.0 1.5437006208763896E-4 0.0 8.247529307156288 0.0 80-81 0.0 1.5437006208763896E-4 0.0 8.961259289218486 0.0 82-83 0.0 1.5437006208763896E-4 0.0 9.654149312898852 0.0 84-85 0.0 1.5437006208763896E-4 0.0 10.366489964402264 0.0 86-87 0.0 1.5437006208763896E-4 0.0 11.123906674035265 0.0 88-89 0.0 1.5437006208763896E-4 0.0 11.932497059250316 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTATACG 20 0.0020661906 71.3149 4 TAGGACG 130 3.009318E-7 29.257399 4 GGTATCA 4480 0.0 23.881449 1 AGGACGT 160 1.8428127E-6 23.771635 5 GTATCAA 5755 0.0 23.217592 1 GGACGTG 150 3.0327645E-5 22.164595 6 GTGTAGC 440 0.0 21.613916 1 GAGTACT 4040 0.0 21.57286 12-13 GTACATG 2500 0.0 21.492878 1 GTATAGC 450 0.0 21.133608 1 AGTACTT 4060 0.0 21.115637 12-13 TAGCCCT 615 0.0 20.872656 4 TACATGG 2530 0.0 20.674183 2 CTCCGAC 175 3.6379788E-12 20.35524 94-95 AGAGTAC 5415 0.0 20.348976 10-11 GTACTTT 4310 0.0 20.221428 14-15 CATGGGT 755 0.0 20.15079 4 ACTATAC 215 1.0679341E-6 19.901834 3 ACAGTAC 215 1.0782696E-6 19.881865 8 GGTGTAC 120 0.0047362773 19.809696 3 >>END_MODULE