FastQCFastQC Report
Thu 26 May 2016
SRR938316_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938316_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635304
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43230.6804616372634203No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35580.5600468437157644No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24570.3867439839824714No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17960.28269930615894123No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12730.2003765126616549No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9560.15047914069484847No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9060.14260889275055721No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6600.10388727286464433No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.10168360344024278No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA300.003953632431.66253330-31
GTATCAA44000.028.8358821
TCGCGGT350.00840207927.1361192-93
GGTATCA31150.027.1031321
TAGCCCT7600.026.8958424
CGATCTG458.901512E-426.41247694-95
GTATAGC5100.026.1873861
TATCAAC50800.024.9849132
ATCAACG50650.024.9650523
TCAACGC50800.024.8913364
ATAGCCC3650.024.7452053
CAACGCA51250.024.5800235
AACGCAG52500.024.0720566
GTACATG24850.023.4173491
TACATGG24200.023.3755512
ACGCAGA58150.021.5663477
GAGTACT33650.021.455912-13
CGCAGAG58400.021.3926858
ACATGGG25550.021.2101733
GTACTAA1353.6880313E-421.199311
Produced by FastQC (version 0.11.5)