##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938316_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 635304 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.848674020626344 33.0 31.0 34.0 31.0 34.0 2 32.05106059461297 33.0 31.0 34.0 31.0 34.0 3 32.10922959716923 34.0 31.0 34.0 30.0 34.0 4 35.75210607834989 37.0 35.0 37.0 35.0 37.0 5 35.77342658003098 37.0 35.0 37.0 35.0 37.0 6 35.782875599712895 37.0 35.0 37.0 35.0 37.0 7 35.75764515885309 37.0 35.0 37.0 35.0 37.0 8 35.745255814539185 37.0 35.0 37.0 35.0 37.0 9 37.37929872942717 39.0 37.0 39.0 35.0 39.0 10-11 37.276051307720394 39.0 37.0 39.0 35.0 39.0 12-13 37.17827843048367 39.0 37.0 39.0 34.0 39.0 14-15 38.46962005591024 40.0 38.0 41.0 34.5 41.0 16-17 38.42141557427625 40.0 38.0 41.0 34.5 41.0 18-19 38.470983969878986 40.0 38.0 41.0 34.5 41.0 20-21 38.45238263886266 40.0 38.0 41.0 34.0 41.0 22-23 38.33111785853701 40.0 38.0 41.0 34.0 41.0 24-25 38.249789864379885 40.0 38.0 41.0 34.0 41.0 26-27 38.079647696221016 40.0 38.0 41.0 34.0 41.0 28-29 37.91098592170047 40.0 38.0 41.0 34.0 41.0 30-31 37.6729628650221 40.0 37.0 41.0 33.0 41.0 32-33 37.393990908289574 39.5 36.5 41.0 33.0 41.0 34-35 37.147482937302456 39.0 36.0 41.0 32.5 41.0 36-37 36.99594839635827 39.0 36.0 41.0 32.0 41.0 38-39 36.82408028282523 39.0 36.0 41.0 31.5 41.0 40-41 36.61238163147092 39.0 36.0 40.0 31.0 41.0 42-43 36.25400595620364 39.0 35.0 40.0 30.0 41.0 44-45 35.80527747346153 39.0 35.0 40.0 29.5 41.0 46-47 35.30455104957626 38.0 35.0 40.0 27.5 41.0 48-49 34.84377321723144 38.0 35.0 40.0 25.0 41.0 50-51 34.1391837608452 37.5 33.5 39.5 24.0 40.5 52-53 34.19004602520998 38.0 34.0 39.5 24.0 40.5 54-55 34.33829001548865 38.0 34.0 40.0 23.0 41.0 56-57 34.073268860262175 38.0 34.0 40.0 21.5 41.0 58-59 33.77574358102578 38.0 34.0 40.0 19.5 41.0 60-61 33.40450792061753 38.0 33.0 40.0 16.0 41.0 62-63 32.86803325651971 37.0 32.5 40.0 10.0 41.0 64-65 32.41104101343609 37.0 32.0 39.0 7.5 40.5 66-67 31.90350525102943 36.0 31.0 39.0 2.0 40.0 68-69 31.24239104428746 35.5 30.5 39.0 2.0 40.0 70-71 30.60917135733444 35.0 29.5 38.0 2.0 40.0 72-73 29.87815203430169 34.5 28.5 37.5 2.0 39.5 74-75 29.11217936609875 34.0 28.0 37.0 2.0 39.0 76-77 28.31543560248322 34.0 26.0 36.0 2.0 39.0 78-79 27.498100122146248 33.0 26.0 35.5 2.0 38.0 80-81 26.6529299359047 32.0 24.0 35.0 2.0 37.0 82-83 25.769545760769645 31.5 21.0 35.0 2.0 36.5 84-85 24.88304181934948 31.0 18.5 34.0 2.0 36.0 86-87 24.002727040912696 30.5 10.0 34.0 2.0 35.5 88-89 23.005697272486874 29.5 4.5 34.0 2.0 35.0 90-91 21.891995013410906 29.0 2.0 34.0 2.0 35.0 92-93 20.729390181708286 28.0 2.0 33.5 2.0 35.0 94-95 19.4097109415335 26.0 2.0 32.5 2.0 34.0 96-97 17.85070454459597 24.0 2.0 32.0 2.0 34.0 98-99 15.872172219913615 9.5 2.0 32.0 2.0 34.0 100-101 11.247185599335122 2.0 2.0 25.0 2.0 31.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20366221417361885 1101 2 0.09667431505909718 1101 3 0.10413204176205682 1101 4 -0.019708563561373182 1101 5 -0.06497495254639318 1101 6 0.05702824212185931 1101 7 0.11533191857711955 1101 8 0.16500098240727823 1101 9 0.36446635157879825 1101 10-11 0.19205296959199103 1101 12-13 0.06920925970677416 1101 14-15 0.11102408032677147 1101 16-17 0.26170786833401394 1101 18-19 0.14962745743753914 1101 20-21 0.2756458742829082 1101 22-23 0.25558254058630325 1101 24-25 0.2714781726658444 1101 26-27 0.3882645437204246 1101 28-29 0.35103346939991553 1101 30-31 0.5627017640993159 1101 32-33 0.4720310581171532 1101 34-35 0.5202583007406858 1101 36-37 0.3935935907316903 1101 38-39 0.38693882550902714 1101 40-41 0.45899381147903995 1101 42-43 0.2965509280000802 1101 44-45 0.1798779161434112 1101 46-47 0.2897227787967509 1101 48-49 0.1395706717538161 1101 50-51 0.1947830167791338 1101 52-53 0.19280308514193933 1101 54-55 0.44666918943972433 1101 56-57 0.6099532480755343 1101 58-59 0.5568621262673048 1101 60-61 0.6352682478645022 1101 62-63 0.41561049474201184 1101 64-65 0.32597437711810784 1101 66-67 0.6193225558872619 1101 68-69 0.6446356430172351 1101 70-71 0.697626911419956 1101 72-73 0.7764626346671868 1101 74-75 1.208115437139515 1101 76-77 1.3468324720246265 1101 78-79 1.4443343142173397 1101 80-81 1.474746167784616 1101 82-83 1.4962180362036648 1101 84-85 1.6558476643356563 1101 86-87 2.025257177287685 1101 88-89 2.23754841533016 1101 90-91 2.2571904956916455 1101 92-93 2.6633165650095627 1101 94-95 2.4754035516079718 1101 96-97 2.6850466635448633 1101 98-99 3.0195327621561727 1101 100-101 2.7238690875509395 1102 1 0.4349845056155459 1102 2 0.20550696795179135 1102 3 0.2362579585504463 1102 4 0.10179009190167676 1102 5 0.19266774643732987 1102 6 0.1454941280452573 1102 7 0.2448074717469737 1102 8 0.31003548616732957 1102 9 0.3118255364952134 1102 10-11 0.3508592994964488 1102 12-13 0.3702487596732382 1102 14-15 0.43873883203905706 1102 16-17 0.3838749212432191 1102 18-19 0.3460988866725714 1102 20-21 0.2991184957944668 1102 22-23 0.24229717358706893 1102 24-25 0.30176638358008034 1102 26-27 0.30044640976222325 1102 28-29 0.24297373170074366 1102 30-31 0.24828786349698362 1102 32-33 0.4078145799265229 1102 34-35 0.43573699202364935 1102 36-37 0.4975461680619162 1102 38-39 0.5361992011535506 1102 40-41 0.4659474675356492 1102 42-43 0.6001285317823246 1102 44-45 0.6264499920981308 1102 46-47 0.775638689752796 1102 48-49 0.7985666820146236 1102 50-51 0.745253475676563 1102 52-53 0.5804465067175002 1102 54-55 0.5566876671451837 1102 56-57 0.900041610734057 1102 58-59 1.1768575326477944 1102 60-61 0.9589855183254627 1102 62-63 0.8637593693052281 1102 64-65 0.9764167065477167 1102 66-67 1.2586614875868527 1102 68-69 1.1761664538168866 1102 70-71 1.3787587483000117 1102 72-73 1.5329375736339834 1102 74-75 1.3781283921788194 1102 76-77 1.6357117837042878 1102 78-79 1.9066330852950877 1102 80-81 2.0834936580683383 1102 82-83 2.742998631516109 1102 84-85 2.4940322555809367 1102 86-87 2.417001307519378 1102 88-89 2.787613706663702 1102 90-91 2.8353517059297424 1102 92-93 2.951146828187831 1102 94-95 3.108769487596657 1102 96-97 3.0983653213821434 1102 98-99 3.5663838101723986 1102 100-101 2.7266226785742855 1103 1 0.42658258770110535 1103 2 0.23648904026128292 1103 3 0.27941103344628004 1103 4 0.14835399211908396 1103 5 0.19069732001054973 1103 6 0.2157178683710299 1103 7 0.282471326323126 1103 8 0.3180251604457851 1103 9 0.4157060251020752 1103 10-11 0.39848448169341566 1103 12-13 0.2403576607004041 1103 14-15 0.5463503709347393 1103 16-17 0.2030619179010955 1103 18-19 0.2466684208863441 1103 20-21 0.13590559649479417 1103 22-23 0.23031756983173324 1103 24-25 0.3848715106149214 1103 26-27 0.2598526807731574 1103 28-29 0.255761872825488 1103 30-31 0.48428630286035457 1103 32-33 0.5541279439818254 1103 34-35 0.5092574545217516 1103 36-37 0.535546197507891 1103 38-39 0.5999043389852474 1103 40-41 0.6960567120991854 1103 42-43 0.7338488342906331 1103 44-45 0.8719410773295024 1103 46-47 1.1162243210990113 1103 48-49 1.012734612404337 1103 50-51 1.0654269124634155 1103 52-53 0.9475261531695054 1103 54-55 1.1572002724758832 1103 56-57 1.0813269765757454 1103 58-59 1.1311978692152778 1103 60-61 1.5779288495485062 1103 62-63 1.6342451787995884 1103 64-65 1.643872279934449 1103 66-67 1.5563946383551475 1103 68-69 1.6610115925319597 1103 70-71 1.722409214773311 1103 72-73 1.8278574069697662 1103 74-75 1.5050650784270623 1103 76-77 1.5764419457062218 1103 78-79 1.8769147360363867 1103 80-81 1.7697713434184728 1103 82-83 1.9341177257579503 1103 84-85 2.146524366513738 1103 86-87 2.1874073675008283 1103 88-89 2.5673504596561507 1103 90-91 2.3576349467536417 1103 92-93 2.5264536797752157 1103 94-95 2.588764629010946 1103 96-97 2.220508448446431 1103 98-99 2.0956837812171916 1103 100-101 2.079181779097933 1104 1 0.3691293436831842 1104 2 0.34635965960978865 1104 3 0.23660614960382276 1104 4 0.2743140535630175 1104 5 0.22159348293198633 1104 6 0.3395400610325936 1104 7 0.3480484717140371 1104 8 0.21328542617495572 1104 9 0.2562843581978669 1104 10-11 0.22892773315428627 1104 12-13 0.30591105199373914 1104 14-15 0.26050147866748574 1104 16-17 0.2966530623735224 1104 18-19 0.2448235193074879 1104 20-21 0.28419450966564597 1104 22-23 0.19635712832223362 1104 24-25 0.18114053368806537 1104 26-27 0.2093356332491254 1104 28-29 0.2249607262943769 1104 30-31 0.36264156914378987 1104 32-33 0.23292503648028173 1104 34-35 0.153832810437315 1104 36-37 0.45133792158029706 1104 38-39 0.5274226692305533 1104 40-41 0.388654935292692 1104 42-43 0.48040844915752956 1104 44-45 0.169199854468566 1104 46-47 0.505902373896447 1104 48-49 0.674262475960802 1104 50-51 0.42404252365634676 1104 52-53 0.7266526358210967 1104 54-55 0.7390727288079404 1104 56-57 0.7811543634666478 1104 58-59 0.7488519145380934 1104 60-61 0.9235747234617548 1104 62-63 0.7963114685679074 1104 64-65 0.8092720613967543 1104 66-67 1.0043573285858791 1104 68-69 1.3598254712593736 1104 70-71 1.3378145388583853 1104 72-73 1.4954188113120175 1104 74-75 1.4415862116558777 1104 76-77 1.462192654370135 1104 78-79 1.504284207270178 1104 80-81 1.819171948356157 1104 82-83 1.8371772535264874 1104 84-85 1.7077545120471775 1104 86-87 2.1909229739429392 1104 88-89 2.4953755439831973 1104 90-91 2.762499785938399 1104 92-93 2.9477484364540736 1104 94-95 3.037622056917261 1104 96-97 3.245481923037463 1104 98-99 3.386085542187624 1104 100-101 2.632858121600348 1105 1 0.5501362401773591 1105 2 0.38162857281743356 1105 3 0.46245694668501613 1105 4 0.2629826088038101 1105 5 0.21886492443695005 1105 6 0.34477188420197535 1105 7 0.14942693612804447 1105 8 0.30074949389661043 1105 9 0.3098479640671883 1105 10-11 0.28855445467026897 1105 12-13 0.44845111465879484 1105 14-15 0.53544755417429 1105 16-17 0.4540312263957702 1105 18-19 0.4367029287039159 1105 20-21 0.42262400313004633 1105 22-23 0.489385118993404 1105 24-25 0.5122828150800487 1105 26-27 0.3097029873400814 1105 28-29 0.4842488812631487 1105 30-31 0.6066900573805327 1105 32-33 0.6705624552806313 1105 34-35 0.7004081357574208 1105 36-37 0.5874352067897561 1105 38-39 0.5105767409991486 1105 40-41 0.5704284565933833 1105 42-43 0.5784091751042908 1105 44-45 0.598981194299256 1105 46-47 0.4689927793001658 1105 48-49 0.46691601995776466 1105 50-51 0.5507567384448038 1105 52-53 0.7784639564183706 1105 54-55 0.7586534736960644 1105 56-57 0.7703725939088528 1105 58-59 1.0123841461568261 1105 60-61 1.1187344526085141 1105 62-63 1.1725072989633816 1105 64-65 1.0498493559517286 1105 66-67 1.107015169853863 1105 68-69 1.4681344325296983 1105 70-71 1.4638758187776126 1105 72-73 1.5851712801568034 1105 74-75 1.8093387389101707 1105 76-77 1.7310787433111265 1105 78-79 2.0408975822133293 1105 80-81 1.9014167092317997 1105 82-83 2.097728514128857 1105 84-85 2.3400259744126792 1105 86-87 2.0966187354986374 1105 88-89 2.542921927391557 1105 90-91 2.680564804066208 1105 92-93 2.7591910678859044 1105 94-95 2.179344386455366 1105 96-97 2.419397279314609 1105 98-99 2.5967488238841714 1105 100-101 2.1162134975698415 1106 1 0.20859915222515468 1106 2 0.14459235474970455 1106 3 -0.029962333459508272 1106 4 0.01658209480012829 1106 5 0.10377315892609573 1106 6 -0.0639945486875817 1106 7 0.19046378835615485 1106 8 -0.18583092139500224 1106 9 0.01327993993571397 1106 10-11 -0.07225047008576979 1106 12-13 0.11887128763438426 1106 14-15 0.023388076605634467 1106 16-17 -0.44487860183856043 1106 18-19 -0.14766543503713336 1106 20-21 -0.03760225027544095 1106 22-23 -0.01203756976318715 1106 24-25 7.281625540471737E-4 1106 26-27 -0.010104720211842277 1106 28-29 0.06811241996153683 1106 30-31 -0.35532603026710774 1106 32-33 -0.2552929311191292 1106 34-35 -0.08180783757446619 1106 36-37 0.002884313332870647 1106 38-39 -0.17434463562954505 1106 40-41 -0.01782518252763765 1106 42-43 -0.2743780015052053 1106 44-45 0.11544433164087309 1106 46-47 0.26584346373445555 1106 48-49 0.13228898553370527 1106 50-51 -0.2004051935748734 1106 52-53 -0.2328466278018837 1106 54-55 -0.23147219019059406 1106 56-57 0.043304584379754374 1106 58-59 0.09568549333042853 1106 60-61 0.25052612257810125 1106 62-63 0.44800513572549505 1106 64-65 0.3983736814262073 1106 66-67 0.46532934625892963 1106 68-69 0.4688092709648224 1106 70-71 0.40629760218680744 1106 72-73 0.5867295624165827 1106 74-75 0.6116893044393805 1106 76-77 0.857627116814907 1106 78-79 0.8709999657385126 1106 80-81 0.6608508879732433 1106 82-83 0.7738488293733212 1106 84-85 0.7585645267747161 1106 86-87 0.6953412360931601 1106 88-89 0.7081885042188354 1106 90-91 0.6262700363065861 1106 92-93 1.3483375380741052 1106 94-95 1.6024232351205008 1106 96-97 1.687308138059553 1106 98-99 1.8750398868120701 1106 100-101 0.7396327902306847 1107 1 0.23301674494171465 1107 2 0.3231324036183594 1107 3 0.2651344833834415 1107 4 0.16067768992665066 1107 5 0.2883464786476111 1107 6 0.1830975988555963 1107 7 0.2201196994553669 1107 8 0.19996160437819555 1107 9 0.4011916416360748 1107 10-11 0.4349679075417541 1107 12-13 0.37842431514810926 1107 14-15 0.46932398670068665 1107 16-17 0.5572656778508218 1107 18-19 0.46542387578091393 1107 20-21 0.4519807343945317 1107 22-23 0.4603482917979349 1107 24-25 0.385142073385488 1107 26-27 0.3142379796140986 1107 28-29 0.40148829022528787 1107 30-31 0.5540849517920705 1107 32-33 0.31761571629016316 1107 34-35 0.5599541536559443 1107 36-37 0.4533169348350796 1107 38-39 0.5987173539272916 1107 40-41 0.4571683323337936 1107 42-43 0.4307563454622567 1107 44-45 0.3265094424321475 1107 46-47 0.26579534819019557 1107 48-49 -0.023847001546229762 1107 50-51 0.08379136051537017 1107 52-53 0.2555073185095438 1107 54-55 0.28292795541123184 1107 56-57 0.10074094634253328 1107 58-59 0.2985333059559494 1107 60-61 0.12217895428248227 1107 62-63 0.023260984902094606 1107 64-65 0.3017325648108624 1107 66-67 0.6665475063205086 1107 68-69 0.489857729593826 1107 70-71 0.6598884861739549 1107 72-73 0.8071102609706067 1107 74-75 0.9061449293557011 1107 76-77 0.9376945204164606 1107 78-79 1.1665172705986002 1107 80-81 1.7905850391313614 1107 82-83 1.7484898455736158 1107 84-85 1.6827961424528546 1107 86-87 1.5548239730726756 1107 88-89 1.6905667091324794 1107 90-91 1.5804368424386013 1107 92-93 1.690091788977842 1107 94-95 1.8163941063884046 1107 96-97 1.3240859864997745 1107 98-99 1.5658891470878817 1107 100-101 1.0758412016646144 1108 1 0.2592971546728293 1108 2 0.1384773139896751 1108 3 0.06925980348587757 1108 4 0.05177884229541263 1108 5 0.07403389862200527 1108 6 0.10904990359311029 1108 7 0.24585593505075565 1108 8 0.1640020973359455 1108 9 0.15969036122634606 1108 10-11 0.020737353764552324 1108 12-13 -0.07605911955099742 1108 14-15 0.056013727417720816 1108 16-17 0.13099727196094335 1108 18-19 0.23397181112022025 1108 20-21 0.08412502056609839 1108 22-23 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-0.04959995234965575 2316 96-97 -0.3137521551911071 2316 98-99 -0.33402393925500284 2316 100-101 -0.16741109873665927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3466.0 3 615.0 4 480.0 5 451.0 6 450.0 7 459.0 8 553.0 9 609.0 10 859.0 11 1338.0 12 2061.0 13 3048.0 14 3812.0 15 5255.0 16 6985.0 17 9249.0 18 8003.0 19 5646.0 20 5491.0 21 5798.0 22 6428.0 23 7436.0 24 8607.0 25 9991.0 26 11803.0 27 14322.0 28 17043.0 29 20823.0 30 25471.0 31 30981.0 32 38637.0 33 47816.0 34 60593.0 35 74071.0 36 87225.0 37 81979.0 38 26970.0 39 480.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.205656282283364 21.088322544568815 15.448784171002709 22.257237002145118 2 14.993709978854822 21.933201392464255 40.76532001958645 22.307768609094474 3 18.514080570623292 25.694738582416804 31.629913634968 24.16126721199191 4 11.94368069137762 18.489595636056706 38.89518788325444 30.67153578931124 5 12.252014439705409 38.26096680505768 35.73197084338716 13.755047911849758 6 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32.24042382162205 19.739606993466165 28-29 19.053096146821346 28.977487435287003 32.18227651185933 19.787139906032323 30-31 19.83559823363757 28.66615072969915 31.949541049661427 19.548709987001853 32-33 19.337907775344306 28.656653907983177 31.98640263382054 20.01903568285198 34-35 19.867851016309608 28.877379189487286 31.78180695982548 19.47296283437763 36-37 19.6007715320422 28.624626043142815 31.74783498661628 20.026767438198707 38-39 19.23311246392965 28.887765913065927 31.959393404143782 19.91972821886064 40-41 20.033216053901313 28.560847560351682 31.653482569442804 19.752453816304204 42-43 19.531794214253498 28.753914899009576 31.33194329329294 20.382347593443985 44-45 20.004108702736147 28.8191651304946 31.130113642624725 20.046612524144532 46-47 19.958861990084685 28.910826711840283 30.692293038655244 20.438018259419785 48-49 19.804187349161445 28.746660495372318 30.958801887282565 20.490350268183672 50-51 19.872883690544594 29.132067155192093 30.48744972412219 20.507599430141127 52-53 19.89501804008488 29.422260772103037 30.089633720161444 20.593087467650633 54-55 19.98611588825651 28.953410304624022 30.279642066969586 20.78083174014988 56-57 20.126011892815118 29.099223543208858 29.94415650346513 20.830608060510894 58-59 20.240050686112085 29.088476386489475 30.00279403257606 20.66867889482238 60-61 20.13002396628192 28.931513102748056 30.242771745760642 20.69569118520938 62-63 19.885318483244454 28.79482083472717 30.39556071548043 20.92429996654794 64-65 19.859161913427155 28.82001862204721 30.157720711793516 21.16309875273211 66-67 19.814083589189426 28.704417147880758 29.99911063608409 21.482388626845726 68-69 20.005336288493293 28.574959112066782 30.110283295528063 21.30942130391186 70-71 20.03645883347475 28.213879229327006 30.130329310162978 21.619332627035266 72-73 19.86619388506055 27.90172334405094 30.57202114136616 21.66006162952235 74-75 19.961179904224874 28.29443452690173 29.835116916809135 21.909268652064267 76-77 19.77625508242861 28.039894408266004 30.081995727847854 22.101854781457536 78-79 19.874554214098445 27.98878244860918 29.89353086046616 22.243132476826215 80-81 19.960235376347132 28.07160465841149 29.639914488742804 22.328245476498573 82-83 20.04455114329568 27.86957377307253 29.930103506631507 22.155771577000273 84-85 20.19590481025411 27.76905428113796 29.706300201099555 22.328740707508373 86-87 19.893576245827695 27.80294998902077 29.810220678571458 22.493253086580076 88-89 20.372064173749592 27.648442333516478 29.39635996114689 22.583133531587045 90-91 20.51347047186603 27.497506488640056 29.33841455974469 22.650608479749224 92-93 20.501982712073055 27.249343162673696 29.63158864116139 22.617085484091863 94-95 20.410973030288478 27.389201881999544 29.262778554903328 22.93704653280865 96-97 20.37911371679744 27.204294134686833 29.31231816521619 23.104273983299535 98-99 20.811294557681773 26.90415021629484 28.89252003954386 23.392035186479525 100-101 21.428627710539587 26.05537695410993 29.281155446293493 23.234839889056985 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1362.0 1 859.0 2 331.0 3 297.5 4 231.0 5 227.0 6 388.5 7 682.5 8 949.5 9 1011.0 10 845.0 11 647.0 12 617.5 13 660.5 14 741.0 15 843.0 16 919.5 17 1040.5 18 1177.5 19 1379.0 20 1695.0 21 1972.5 22 2301.0 23 2753.0 24 3391.5 25 4192.5 26 5137.0 27 6451.5 28 7900.0 29 9435.0 30 11195.0 31 12786.0 32 14441.5 33 16524.5 34 18503.5 35 20312.5 36 22009.0 37 23141.0 38 23568.0 39 23716.0 40 23764.0 41 23693.0 42 23686.0 43 23960.5 44 23817.5 45 23506.5 46 23701.5 47 24254.5 48 25121.0 49 24349.0 50 22110.0 51 20103.5 52 17902.5 53 15741.0 54 13744.5 55 12083.5 56 10615.5 57 8776.5 58 7003.0 59 5547.0 60 4251.5 61 3106.5 62 2279.0 63 1642.5 64 1161.0 65 836.0 66 606.0 67 444.0 68 334.0 69 260.0 70 197.0 71 158.5 72 143.5 73 117.5 74 86.5 75 65.5 76 55.5 77 39.5 78 25.5 79 18.0 80 11.5 81 8.0 82 6.5 83 4.5 84 2.5 85 1.5 86 0.5 87 1.0 88 1.0 89 1.5 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35227229798647575 2 0.026916247969476028 3 0.03289763640713737 4 0.044388198405802576 5 0.061073124047699996 6 0.05210104139120799 7 0.035573520708196395 8 0.037147570297054636 9 0.0317958016949366 10-11 0.03518000831098183 12-13 0.026129223175046908 14-15 0.023689446312316623 16-17 0.020541347134600128 18-19 0.024791281024517393 20-21 0.03313374384546611 22-23 0.031717099215493684 24-25 0.042027124022515204 26-27 0.03549481822875348 28-29 0.030221752106078352 30-31 0.03360595872212358 32-33 0.028490297558334276 34-35 0.029513429791092137 36-37 0.03211061161270825 38-39 0.013694231423066753 40-41 0.010860942163121907 42-43 0.011097049601450644 44-45 0.010546132245350257 46-47 0.01967561986072809 48-49 0.0165275206830116 50-51 0.009286892574263659 52-53 0.008185057862062887 54-55 0.007161925629305025 56-57 0.007319330588190851 58-59 0.003384206616045232 60-61 0.005745280999332603 62-63 0.010467429765907345 64-65 0.005587876040446778 66-67 0.0029119917393877577 68-69 0.004879553725460567 70-71 0.010546132245350257 72-73 0.008893380177049099 74-75 0.010388727286464432 76-77 0.004249933889917268 78-79 0.007791545464848325 80-81 0.010388727286464432 82-83 0.012434991751980153 84-85 0.007319330588190851 86-87 0.002124966944958634 88-89 0.01432385125861005 90-91 0.024476471106745747 92-93 0.009916512409806958 94-95 0.02022653721682848 96-97 0.01762935539521237 98-99 0.009444297533149485 100-101 0.11710928941105361 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 635304.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.78111135208593 #Duplication Level Percentage of deduplicated Percentage of total 1 82.43295027875779 60.81994683597992 2 12.071719535265853 17.81329766485202 3 3.2242488924962522 7.136659996923171 4 1.1376709485141672 3.357545077374279 5 0.4750164101000407 1.752361932382961 6 0.23239896342673946 1.0287992279218565 7 0.1223563291825657 0.6319310163635953 8 0.07103363941488813 0.4192752687531025 9 0.046162991609766904 0.30653611418750587 >10 0.16598844599252327 2.1006059823118344 >50 0.008735415924709224 0.46310220825869514 >100 0.009587576712218559 1.4834587882118424 >500 0.0010652863012071006 0.5789529211177938 >1k 0.0010652863012071006 2.1075269653614614 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4323 0.6804616372634203 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3558 0.5600468437157644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2457 0.3867439839824714 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1796 0.28269930615894123 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1273 0.2003765126616549 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 956 0.15047914069484847 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 906 0.14260889275055721 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 660 0.10388727286464433 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 646 0.10168360344024278 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.5740495888582473E-4 0.0 16-17 0.0 0.0 0.0 3.1480991777164946E-4 0.0 18-19 0.0 0.0 0.0 3.1480991777164946E-4 0.0 20-21 0.0 0.0 0.0 3.1480991777164946E-4 0.0 22-23 0.0 0.0 0.0 9.444297533149484E-4 0.0 24-25 0.0 0.0 0.0 0.0018101570271869846 0.0 26-27 0.0 0.0 0.0 0.005430471081560953 0.0 28-29 0.0 0.0 0.0 0.013694231423066751 0.0 30-31 0.0 0.0 0.0 0.03636054550262552 0.0 32-33 0.0 0.0 0.0 0.08342462820948711 0.0 34-35 0.0 0.0 0.0 0.17314545477440724 0.0 36-37 0.0 0.0 0.0 0.30316195081409847 0.0 38-39 0.0 0.0 0.0 0.4634789014393109 0.0 40-41 0.0 0.0 0.0 0.623323637187866 0.0 42-43 0.0 0.0 0.0 0.7937932076612142 0.0 44-45 0.0 0.0 0.0 1.0170721418407564 0.0 46-47 0.0 0.0 0.0 1.283322629796129 0.0 48-49 0.0 0.0 0.0 1.540286225177238 0.0 50-51 0.0 0.0 0.0 1.792606374271215 0.0 52-53 0.0 0.0 0.0 2.0417784241874757 0.0 54-55 0.0 0.0 0.0 2.3511578708775644 0.0 56-57 0.0 0.0 0.0 2.717596615163764 0.0 58-59 0.0 0.0 0.0 3.0891510206137536 0.0 60-61 0.0 0.0 0.0 3.46739513681639 0.0 62-63 0.0 0.0 0.0 3.8524863687305606 0.0 64-65 0.0 0.0 0.0 4.271655774243512 0.0 66-67 0.0 0.0 0.0 4.770157279034919 0.0 68-69 0.0 0.0 0.0 5.312338659917142 0.0 70-71 0.0 0.0 0.0 5.863964338332515 0.0 72-73 0.0 0.0 0.0 6.416849256418974 0.0 74-75 0.0 0.0 0.0 7.002159596035913 0.0 76-77 0.0 0.0 0.0 7.6397283820029465 0.0 78-79 0.0 0.0 0.0 8.367647614370442 0.0 80-81 0.0 0.0 0.0 9.109261078161007 0.0 82-83 0.0 0.0 0.0 9.833638698953571 0.0 84-85 0.0 0.0 0.0 10.579895609031269 0.0 86-87 0.0 0.0 0.0 11.3601519902283 0.0 88-89 0.0 0.0 0.0 12.174722652462442 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTACGA 30 0.0039536324 31.662533 30-31 GTATCAA 4400 0.0 28.835882 1 TCGCGGT 35 0.008402079 27.13611 92-93 GGTATCA 3115 0.0 27.103132 1 TAGCCCT 760 0.0 26.895842 4 CGATCTG 45 8.901512E-4 26.412476 94-95 GTATAGC 510 0.0 26.187386 1 TATCAAC 5080 0.0 24.984913 2 ATCAACG 5065 0.0 24.965052 3 TCAACGC 5080 0.0 24.891336 4 ATAGCCC 365 0.0 24.745205 3 CAACGCA 5125 0.0 24.580023 5 AACGCAG 5250 0.0 24.072056 6 GTACATG 2485 0.0 23.417349 1 TACATGG 2420 0.0 23.375551 2 ACGCAGA 5815 0.0 21.566347 7 GAGTACT 3365 0.0 21.4559 12-13 CGCAGAG 5840 0.0 21.392685 8 ACATGGG 2555 0.0 21.210173 3 GTACTAA 135 3.6880313E-4 21.19931 1 >>END_MODULE