FastQCFastQC Report
Thu 26 May 2016
SRR938316_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938316_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences635304
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42100.6626748769093221No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39770.6259995214889249No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26770.4213730749373528No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18840.2965509425408938No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14790.23280193419213477No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9780.1539420497903366No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT9520.14984952085930514RNA PCR Primer, Index 39 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT9320.14670142168158867No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7120.11207233072670722No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT6530.10278543815244355No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37950.028.1723021
TCGTATC601.7113004E-423.74929294-95
ACACCGC1000.001653121623.749296
GTAAGCG500.001654028823.7492994-95
GTATCAA49700.023.4240111
CTCCGAC2850.022.49932994-95
GAGTACT35900.022.16159412-13
ATGGGTG3700.021.8236715
CATGGGT8200.021.4322874
GTAGGAC2551.189801E-820.4895863
ATAGCCC3750.020.2660623
GTACTTT38800.020.2603514-15
AGTACTT36850.020.17239812-13
GTACATG23600.020.1344411
TATCAAC58800.019.7941912
TAGCCCT6750.019.7031154
ATCAACG59200.019.4968833
TACATGG24350.019.314582
TCAACGC59900.019.2690414
AGAGTAC49150.019.18304810-11
Produced by FastQC (version 0.11.5)