##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938316_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 635304 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.3657744953597 31.0 31.0 34.0 30.0 34.0 2 31.653639832269278 31.0 31.0 34.0 30.0 34.0 3 31.67787075164016 31.0 31.0 34.0 30.0 34.0 4 35.42070882601085 37.0 35.0 37.0 33.0 37.0 5 35.31705136438618 37.0 35.0 37.0 33.0 37.0 6 35.35926894840895 37.0 35.0 37.0 33.0 37.0 7 35.27644875524159 37.0 35.0 37.0 32.0 37.0 8 35.26337942150529 37.0 35.0 37.0 32.0 37.0 9 36.69474298918313 39.0 35.0 39.0 32.0 39.0 10-11 36.62440894437938 38.0 35.0 39.0 32.0 39.0 12-13 36.499722180247566 38.0 35.0 39.0 32.0 39.0 14-15 37.31570004281415 39.0 36.0 40.0 32.0 41.0 16-17 37.386544866709485 39.0 36.0 40.0 32.0 41.0 18-19 37.58455715688867 39.0 36.0 41.0 32.0 41.0 20-21 37.567852555626914 39.0 36.0 41.0 32.0 41.0 22-23 37.389005106216864 39.0 36.0 41.0 32.0 41.0 24-25 37.23344808154836 39.0 36.0 40.5 31.0 41.0 26-27 36.943349955296995 39.0 36.0 40.0 31.0 41.0 28-29 36.759571795549846 39.0 35.5 40.0 30.5 41.0 30-31 36.42648480097717 38.0 35.0 40.0 30.0 41.0 32-33 36.17506658229761 38.0 35.0 40.0 30.0 41.0 34-35 35.9272017805649 38.0 35.0 40.0 30.0 41.0 36-37 35.72998359840328 38.0 34.5 40.0 29.0 41.0 38-39 35.43446444536789 38.0 34.0 40.0 27.5 41.0 40-41 35.141090721922104 38.0 34.0 40.0 27.0 41.0 42-43 34.7559758792641 38.0 33.5 40.0 25.5 41.0 44-45 34.28781103219876 38.0 33.0 40.0 24.0 41.0 46-47 33.657028761034084 37.5 33.0 40.0 22.5 41.0 48-49 33.08477358870714 37.0 32.0 40.0 18.5 41.0 50-51 32.56606207421959 37.0 31.0 39.0 15.0 40.0 52-53 31.85936811353305 36.0 30.0 39.0 10.5 40.0 54-55 31.336804899701562 36.0 30.0 39.0 8.5 40.0 56-57 30.84163801896415 35.0 28.5 38.5 6.5 40.0 58-59 30.189233028597332 35.0 28.0 38.0 2.0 40.0 60-61 29.5707078815811 34.0 27.0 38.0 2.0 40.0 62-63 28.92957544734489 34.0 26.0 38.0 2.0 40.0 64-65 28.15330141160767 33.0 24.5 37.5 2.0 39.0 66-67 27.36924763577752 32.5 22.5 37.0 2.0 39.0 68-69 26.512720681752356 31.5 21.5 36.0 2.0 39.0 70-71 25.612443019404886 31.0 18.5 35.5 2.0 38.5 72-73 24.917655956833265 30.5 15.5 35.0 2.0 38.0 74-75 24.113243423620816 30.0 10.5 34.5 2.0 37.0 76-77 23.17659970659716 28.5 8.0 33.5 2.0 36.0 78-79 23.558382286275545 30.0 7.5 34.0 2.0 36.0 80-81 23.04597169229219 29.0 4.0 34.0 2.0 36.0 82-83 22.198282239683678 29.0 2.0 34.0 2.0 35.5 84-85 21.227270723936886 28.5 2.0 33.5 2.0 35.0 86-87 20.10107759434853 26.0 2.0 33.0 2.0 35.0 88-89 18.911045735584853 25.5 2.0 32.0 2.0 34.0 90-91 17.83797756664526 23.0 2.0 32.0 2.0 34.0 92-93 16.567796676866507 19.0 2.0 31.0 2.0 34.0 94-95 15.216885931774396 4.5 2.0 31.0 2.0 34.0 96-97 13.74124513618677 2.0 2.0 30.0 2.0 34.0 98-99 12.029230887889891 2.0 2.0 29.0 2.0 34.0 100-101 8.993999722967272 2.0 2.0 13.5 2.0 29.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2913291419720494 1101 2 0.1810004447070206 1101 3 0.07735889120497674 1101 4 0.1214414137214419 1101 5 0.057837614376495594 1101 6 0.047350062602070864 1101 7 0.02742704553043751 1101 8 0.23748593031223209 1101 9 0.2418672376985711 1101 10-11 0.39103626747340314 1101 12-13 0.36245169990431236 1101 14-15 0.2587511543032406 1101 16-17 0.2263942455756407 1101 18-19 0.4263364107009835 1101 20-21 0.46799870854559344 1101 22-23 0.3658978262521373 1101 24-25 0.3423057792210855 1101 26-27 0.23618669146448212 1101 28-29 0.4925010928955942 1101 30-31 0.5183400548560755 1101 32-33 0.39791344899219894 1101 34-35 0.23987487157425136 1101 36-37 0.654934200856566 1101 38-39 0.6205148117291088 1101 40-41 0.7225834092391068 1101 42-43 0.7566445856803625 1101 44-45 0.6525556800489625 1101 46-47 0.5507003206033474 1101 48-49 0.40441485686037026 1101 50-51 0.8040192803651962 1101 52-53 0.6002624724726395 1101 54-55 0.8441391164703411 1101 56-57 1.091990133982236 1101 58-59 1.2423035801057978 1101 60-61 1.0980012453866088 1101 62-63 1.2444802089283122 1101 64-65 1.133001296854271 1101 66-67 1.2817718006941696 1101 68-69 1.593810693089825 1101 70-71 1.8297012700519133 1101 72-73 2.3973714807954103 1101 74-75 2.5236166237710087 1101 76-77 2.4355087149238805 1101 78-79 2.4617512027725894 1101 80-81 2.7293739661834664 1101 82-83 2.8193497477931224 1101 84-85 2.92183667940915 1101 86-87 2.9323115286506507 1101 88-89 2.8254245028672855 1101 90-91 3.1447220055515324 1101 92-93 3.3617003886378356 1101 94-95 2.825166580110663 1101 96-97 2.646377347236962 1101 98-99 2.1019836313713416 1101 100-101 1.8230881907036594 1102 1 0.16442137049687844 1102 2 0.2134293339285307 1102 3 0.16171890710199577 1102 4 0.24948468664953793 1102 5 0.3399142400790893 1102 6 0.27130449802558587 1102 7 0.1710679793255423 1102 8 0.23233746349726658 1102 9 0.15510976532480214 1102 10-11 0.30009034314941374 1102 12-13 0.20770865872174227 1102 14-15 0.35423650375381044 1102 16-17 0.11925194143673679 1102 18-19 0.27015721824896843 1102 20-21 0.40883005045024845 1102 22-23 0.5175853362522886 1102 24-25 0.46952323291068865 1102 26-27 0.8536698008803683 1102 28-29 0.6474828474517906 1102 30-31 0.848605813809769 1102 32-33 0.8169444533432255 1102 34-35 0.7648597745662968 1102 36-37 0.9043174171128143 1102 38-39 0.532519900840164 1102 40-41 1.1151004546643222 1102 42-43 1.4444707197624425 1102 44-45 1.5610871669881092 1102 46-47 1.489034849654118 1102 48-49 1.2526267001405316 1102 50-51 1.4723573707614293 1102 52-53 1.4601170766729616 1102 54-55 1.3534115181075315 1102 56-57 1.6195183021603299 1102 58-59 1.7241871705613896 1102 60-61 1.968183751438314 1102 62-63 2.630935225005981 1102 64-65 2.495565620430895 1102 66-67 2.5084282134386235 1102 68-69 2.5279954804671796 1102 70-71 2.5532866675032295 1102 72-73 2.4299913407158193 1102 74-75 2.8173618172449686 1102 76-77 2.9070127898256892 1102 78-79 3.240894879835974 1102 80-81 3.3577733736581585 1102 82-83 3.468509476717955 1102 84-85 3.6341122243208126 1102 86-87 3.54198161386487 1102 88-89 3.287351655287708 1102 90-91 3.8104613460316408 1102 92-93 3.4275865701012904 1102 94-95 3.221848851320024 1102 96-97 3.6449179698014387 1102 98-99 3.2247390804879714 1102 100-101 2.720543377106546 1103 1 0.260120785994296 1103 2 0.4036503259633939 1103 3 0.3081905432675427 1103 4 0.3371453512451694 1103 5 0.24821702321439432 1103 6 0.3118344274043281 1103 7 0.2800966057199332 1103 8 0.23328709615874033 1103 9 0.4940231107309856 1103 10-11 0.32930074935662645 1103 12-13 0.34811933190578515 1103 14-15 0.6480999627723776 1103 16-17 0.5925837043181232 1103 18-19 0.48403191581428473 1103 20-21 0.6067376686144854 1103 22-23 0.6231074379096029 1103 24-25 0.6147262383630974 1103 26-27 0.5942998465706992 1103 28-29 0.46980952108733476 1103 30-31 0.6641341481982437 1103 32-33 0.5732544801881403 1103 34-35 0.5856061809305473 1103 36-37 0.719591779975552 1103 38-39 0.7335905073781532 1103 40-41 0.6735163594164177 1103 42-43 0.8324692769097126 1103 44-45 0.8228876410682915 1103 46-47 0.9047089416138974 1103 48-49 1.06754403056852 1103 50-51 1.0925092138340204 1103 52-53 1.1686424222950826 1103 54-55 1.0297315565835952 1103 56-57 1.2734569465658296 1103 58-59 1.4401513053650596 1103 60-61 1.921787938655818 1103 62-63 1.8322499877450404 1103 64-65 2.1733542768601772 1103 66-67 2.4368916734387227 1103 68-69 2.300216148596327 1103 70-71 2.2601078273801463 1103 72-73 2.3122148062124985 1103 74-75 2.3488972284825387 1103 76-77 2.699754848001117 1103 78-79 2.3982897454396443 1103 80-81 2.7588005473844994 1103 82-83 3.4104113431019414 1103 84-85 3.3523400183756706 1103 86-87 3.0126080278850793 1103 88-89 3.1203169139460556 1103 90-91 3.2200106753995854 1103 92-93 3.206188426179189 1103 94-95 3.429695712624577 1103 96-97 3.75626669356385 1103 98-99 3.5437297706525257 1103 100-101 2.679533723734737 1104 1 0.3825018967358993 1104 2 0.26810944516145696 1104 3 0.3520406074036089 1104 4 0.4396205282131973 1104 5 0.3292026599033946 1104 6 0.28293360867976247 1104 7 0.425313322270199 1104 8 0.46778754748659424 1104 9 0.2093381692452425 1104 10-11 0.3587558303580991 1104 12-13 0.4231189360041583 1104 14-15 0.5121552623115448 1104 16-17 0.5409818863378533 1104 18-19 0.6221936653827527 1104 20-21 0.551446047476503 1104 22-23 0.6785178295354939 1104 24-25 0.5225682577781114 1104 26-27 0.6188219933076766 1104 28-29 0.5371286336342607 1104 30-31 0.7862302097688669 1104 32-33 1.0244239735924197 1104 34-35 0.8755645320794585 1104 36-37 0.6807775845268367 1104 38-39 1.0060420886092558 1104 40-41 1.1804921460729432 1104 42-43 0.8018264910291535 1104 44-45 0.9837028816972975 1104 46-47 1.438843579838995 1104 48-49 1.6520136015695144 1104 50-51 1.6145134262273189 1104 52-53 1.7196899779984385 1104 54-55 1.559218309715181 1104 56-57 1.8697006319348901 1104 58-59 1.846037570745839 1104 60-61 1.9746170516778143 1104 62-63 2.5991510281745143 1104 64-65 2.8835622043943445 1104 66-67 2.450447345125422 1104 68-69 2.8155612956341933 1104 70-71 2.8474516441885456 1104 72-73 2.8491286738546684 1104 74-75 3.0505615989197707 1104 76-77 3.231783289573954 1104 78-79 3.6254660166797628 1104 80-81 3.58897458573945 1104 82-83 3.4839698652803506 1104 84-85 3.8964988857803 1104 86-87 3.953499915068953 1104 88-89 3.665837682945373 1104 90-91 4.261105264216155 1104 92-93 4.4055011409558205 1104 94-95 4.240985243401971 1104 96-97 4.1712428602859415 1104 98-99 3.691021011724944 1104 100-101 2.6872617234813596 1105 1 0.20317635968132208 1105 2 0.20956017048239772 1105 3 0.2975966867904738 1105 4 0.20106357685580178 1105 5 0.1823736704296195 1105 6 0.013559383127272895 1105 7 0.2344227024201473 1105 8 0.009360515565795424 1105 9 0.12086653749216936 1105 10-11 0.3327406522299512 1105 12-13 0.5388751096806601 1105 14-15 0.3248829029428677 1105 16-17 0.4620915001258439 1105 18-19 0.5175279972405491 1105 20-21 0.44251439911843704 1105 22-23 0.38900692573178475 1105 24-25 0.4662468886592208 1105 26-27 0.5352285985887306 1105 28-29 0.5634473326041629 1105 30-31 0.5279381455350958 1105 32-33 0.5221074107416968 1105 34-35 0.5179460546484691 1105 36-37 0.6830419960301697 1105 38-39 0.5793616678938989 1105 40-41 0.9656557135588812 1105 42-43 0.6319758107669742 1105 44-45 0.5296179709595492 1105 46-47 0.6665593091025741 1105 48-49 0.8300144944005865 1105 50-51 0.9791566901506883 1105 52-53 0.6095505044349956 1105 54-55 0.9331705891462683 1105 56-57 1.0279956166441195 1105 58-59 1.4482615128036045 1105 60-61 1.2101784296415765 1105 62-63 1.6184407685442501 1105 64-65 1.6225760015548083 1105 66-67 1.8704325758638412 1105 68-69 2.1706160128278675 1105 70-71 2.224462078630129 1105 72-73 2.422268852504324 1105 74-75 2.324294634086467 1105 76-77 2.304803520124633 1105 78-79 2.479400522931666 1105 80-81 2.4209742352823973 1105 82-83 2.834330961209627 1105 84-85 3.0447526500645523 1105 86-87 2.9915693556310217 1105 88-89 3.1927811550569167 1105 90-91 3.3866272992034503 1105 92-93 2.910424228148699 1105 94-95 2.5446343774810014 1105 96-97 2.438064798897548 1105 98-99 2.2638628846675726 1105 100-101 1.9586010118032124 1106 1 -0.2493095907456926 1106 2 -0.045587167504226755 1106 3 -0.022038921315097326 1106 4 0.018412869892138417 1106 5 -0.018367295913982673 1106 6 -0.07052736333790932 1106 7 -0.1608427896591067 1106 8 -0.18611525074609858 1106 9 -0.26341588082838996 1106 10-11 -0.042569648286971074 1106 12-13 -0.14904601244944615 1106 14-15 -0.11155607789463318 1106 16-17 -0.11439366594490963 1106 18-19 0.07874742090557163 1106 20-21 -0.15253038845869327 1106 22-23 0.101934618931665 1106 24-25 -0.1924538335326318 1106 26-27 -0.25378478136094884 1106 28-29 -0.05491849449534669 1106 30-31 0.11023610078506607 1106 32-33 -0.10812389091420727 1106 34-35 0.01694892088652722 1106 36-37 -0.2919764655468029 1106 38-39 -0.062440974719173425 1106 40-41 0.12673662324525736 1106 42-43 -0.069307529589409 1106 44-45 0.10815059892852474 1106 46-47 -0.37485303282669946 1106 48-49 -0.016616737163907658 1106 50-51 0.4120097443421997 1106 52-53 0.28301840215160823 1106 54-55 0.023135835488375278 1106 56-57 0.1907610148509491 1106 58-59 0.29964581816851776 1106 60-61 0.6101104242845885 1106 62-63 0.8252187748608222 1106 64-65 0.8828258273693059 1106 66-67 0.905528346598178 1106 68-69 0.8295524591098946 1106 70-71 0.6913397148807405 1106 72-73 0.9470668181845632 1106 74-75 0.9710328802157946 1106 76-77 1.2089556017978147 1106 78-79 1.495863660273283 1106 80-81 1.5293280467114663 1106 82-83 1.3720405574747545 1106 84-85 1.7867787090498126 1106 86-87 1.825370312712547 1106 88-89 1.6709923221206324 1106 90-91 1.9113261773236658 1106 92-93 1.8362817006023562 1106 94-95 1.4280250077613257 1106 96-97 1.3641736408455891 1106 98-99 1.054054885068096 1106 100-101 0.950148321419503 1107 1 0.07262733186777837 1107 2 0.0599657846982069 1107 3 0.0829994037618853 1107 4 0.24546384726527748 1107 5 0.001891440195265659 1107 6 0.052953859047953244 1107 7 0.10375295608999124 1107 8 0.32678180709296356 1107 9 0.33757237061089285 1107 10-11 0.23595659351246923 1107 12-13 0.28962758843420033 1107 14-15 0.3578330220518069 1107 16-17 0.39410081959532306 1107 18-19 0.1590282064859707 1107 20-21 0.31390692744705007 1107 22-23 0.3208069997724792 1107 24-25 0.5342174494369729 1107 26-27 0.29782959974762946 1107 28-29 0.24606829255780127 1107 30-31 0.24641250492216216 1107 32-33 0.13688255238476188 1107 34-35 0.33371551447335435 1107 36-37 0.5299709169675921 1107 38-39 0.5679155405439147 1107 40-41 0.6292051598632824 1107 42-43 0.5719304206047298 1107 44-45 0.733513557490582 1107 46-47 0.6751484315594354 1107 48-49 0.35588648448557336 1107 50-51 0.45991818255347283 1107 52-53 0.701594349400235 1107 54-55 0.6702114563880919 1107 56-57 0.9172283297525397 1107 58-59 1.5037739226671114 1107 60-61 1.5089004229457217 1107 62-63 1.429194374003945 1107 64-65 1.475949279303972 1107 66-67 1.7230030754775427 1107 68-69 1.873127360368425 1107 70-71 1.6873471706981178 1107 72-73 2.0094256095220935 1107 74-75 2.02172574405299 1107 76-77 2.329731893804773 1107 78-79 2.231786725610011 1107 80-81 2.212964159310207 1107 82-83 2.5049806682660254 1107 84-85 2.5364230724184047 1107 86-87 2.8051900258602274 1107 88-89 2.9879942634782424 1107 90-91 3.309188382772856 1107 92-93 3.1994243569151166 1107 94-95 2.8934424833269947 1107 96-97 3.1077910093018755 1107 98-99 3.1910293796456823 1107 100-101 2.2946974578500825 1108 1 -0.011171483753223299 1108 2 -0.11772307573713192 1108 3 0.1118966508809649 1108 4 0.09047825187475667 1108 5 0.13887927630282348 1108 6 0.053746112569079685 1108 7 -0.05996743443498076 1108 8 -0.11870874988526481 1108 9 -0.29620643860805984 1108 10-11 -0.025327017242979366 1108 12-13 0.02752371519476071 1108 14-15 -0.04539000931823267 1108 16-17 0.027909638417348503 1108 18-19 -0.14547523461517642 1108 20-21 0.010231751390705313 1108 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0.8099974308594327 2203 36-37 0.8480124433424479 2203 38-39 0.9154972605214695 2203 40-41 0.8839049353963873 2203 42-43 1.2208852466428866 2203 44-45 0.908062870060732 2203 46-47 1.0722005499657215 2203 48-49 1.3704505276954464 2203 50-51 1.1592903767173368 2203 52-53 1.557339298895183 2203 54-55 1.7038902582960667 2203 56-57 1.7472472691464773 2203 58-59 1.5305784260341149 2203 60-61 1.7297898730018382 2203 62-63 1.7532333751262819 2203 64-65 1.9226822338494252 2203 66-67 2.0974068352979707 2203 68-69 2.047887110649011 2203 70-71 1.9177676953894753 2203 72-73 1.9205595180518351 2203 74-75 2.0268463950069737 2203 76-77 1.7244604291583094 2203 78-79 2.2027287850937363 2203 80-81 2.103483940454989 2203 82-83 2.1097698798260716 2203 84-85 2.2529985494105453 2203 86-87 2.314686027543729 2203 88-89 3.126532422849735 2203 90-91 2.79636045067409 2203 92-93 2.4748171739746 2203 94-95 1.9223601320363084 2203 96-97 2.1535436748462065 2203 98-99 2.6119048005211045 2203 100-101 1.70126311441574 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1.1095782540690813 2316 90-91 1.2162826559844255 2316 92-93 1.5499588110454638 2316 94-95 1.5347290648961103 2316 96-97 1.6780764839109796 2316 98-99 1.2882429537428184 2316 100-101 1.167111668449225 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 7.0 4 15.0 5 41.0 6 106.0 7 270.0 8 563.0 9 972.0 10 1690.0 11 2501.0 12 3573.0 13 4769.0 14 6541.0 15 8911.0 16 11133.0 17 13217.0 18 13107.0 19 11477.0 20 11847.0 21 12677.0 22 13792.0 23 15426.0 24 17035.0 25 18964.0 26 21109.0 27 23968.0 28 27362.0 29 32156.0 30 35498.0 31 39718.0 32 44222.0 33 49038.0 34 53970.0 35 55337.0 36 50095.0 37 29441.0 38 4719.0 39 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.69441011390741 22.823990616611432 16.357167035337277 21.12443223414388 2 15.971664698937428 23.874222746950018 38.75796930342385 21.396143250688702 3 19.00033685191423 26.802551259731715 32.24270967457586 21.954402213778188 4 12.868957223628374 20.38142369637213 37.604202082782415 29.145416997217083 5 12.03632288266053 39.33546565255579 35.387107234039874 13.241104230743803 6 28.951494087869744 39.107262035183155 17.792899147494744 14.148344729452356 7 24.97575963633158 35.084935715814794 22.18607154999811 17.753233097855514 8 24.058403535944997 36.4228778663443 20.874101217684764 18.64461738002594 9 24.47796481043661 17.33821080368077 22.142540083298968 36.041284302583655 10-11 23.180949592635965 28.490061450895947 28.62708246760606 19.701906488862026 12-13 24.573668668857742 26.45331998539282 28.578286930351453 20.394724415397985 14-15 21.22975772228728 28.36311435155453 28.06066072305542 22.346467203102765 16-17 19.53505723244305 30.847279412690614 29.19004130306121 20.42762205180512 18-19 18.965015173838037 29.30809502222558 32.189235389671715 19.537654414264665 20-21 20.578258597458856 27.982115648571394 32.315946381574804 19.123679372394946 22-23 19.760775943485324 28.652031153589462 31.91165174467657 19.67554115824865 24-25 19.68038470316939 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20.238027778827146 50-51 20.169399216752925 29.008632087945298 30.553246949491896 20.26872174580988 52-53 20.263212572248875 29.39852417110549 30.00941281654137 20.328850440104265 54-55 20.305160993791947 29.02515960862831 29.95762658506794 20.712052812511807 56-57 20.56251810157027 29.292354526337 29.537827559719442 20.607299812373288 58-59 20.44517270472089 29.412218402528556 29.5982710639316 20.54433782881896 60-61 20.33648458061023 29.44062999760744 29.703181469029 20.519703952753325 62-63 20.038280886001033 29.402537997557076 29.724352435999148 20.834828680442747 64-65 20.051109390150227 29.43811151826527 29.527911047309637 20.982868044274866 66-67 20.01909338680951 29.234918703570056 29.305908954609528 21.440078955010915 68-69 20.13582655630333 28.94487683290689 29.4017508387829 21.517545772006883 70-71 20.061262009998362 28.73514097188118 29.454796443907167 21.74880057421329 72-73 19.941712714356886 28.347642812113616 29.796010240806925 21.914634232722573 74-75 19.968944001611828 28.67328082303905 29.06506176570587 22.29271340964326 76-77 19.462650951355574 28.336355508543942 29.87317882462569 22.327814715474798 78-79 19.370883860325137 28.350994169720323 29.656589601198796 22.621532368755744 80-81 19.46244547877155 28.316488352801734 29.53984161888107 22.68122454954565 82-83 19.383468978657998 28.21737566632273 29.68330535427159 22.715850000747682 84-85 19.36082295716782 28.110016213578476 29.584113841358793 22.94504698789491 86-87 19.09093341812561 28.069601025063047 29.63104341721262 23.208422139598728 88-89 19.219775099794745 28.081595582587234 29.070177426869652 23.628451890748366 90-91 19.16799413186924 27.672612451518663 29.398973077116807 23.76042033949529 92-93 19.13074069789131 27.564636836641615 29.485699264677528 23.81892320078955 94-95 18.905437396899753 27.630079458023243 29.240883104781336 24.223600040295672 96-97 18.61393806267398 27.547778345310547 29.303317233416788 24.534966358598687 98-99 18.805013033130596 27.313695490662738 29.05711281528213 24.824178660924535 100-101 18.747953735534484 26.969214738141112 29.419459030637302 24.863372495687106 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1544.0 1 922.0 2 312.5 3 291.5 4 203.5 5 200.0 6 366.0 7 683.5 8 938.5 9 983.5 10 798.5 11 604.0 12 600.0 13 635.0 14 718.0 15 790.5 16 888.0 17 1036.0 18 1178.0 19 1373.5 20 1649.0 21 1988.0 22 2398.0 23 2882.5 24 3479.0 25 4316.0 26 5306.5 27 6593.5 28 8026.0 29 9610.0 30 11306.5 31 13016.5 32 14901.0 33 16967.0 34 18942.5 35 20645.5 36 22088.5 37 23146.0 38 23551.0 39 23675.5 40 23899.0 41 23922.0 42 24064.0 43 24393.5 44 24169.0 45 23916.5 46 24106.0 47 24352.5 48 24968.0 49 24378.5 50 22260.5 51 20066.5 52 17683.0 53 15242.5 54 13277.0 55 11655.5 56 9946.0 57 8152.5 58 6560.5 59 5213.5 60 3976.5 61 2909.5 62 2119.5 63 1537.5 64 1078.5 65 737.5 66 527.5 67 387.0 68 283.0 69 195.0 70 141.0 71 107.5 72 77.0 73 61.0 74 51.0 75 34.5 76 24.0 77 20.0 78 13.0 79 6.5 80 3.5 81 2.0 82 1.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02187928928512964 2 0.008499867779834537 3 0.0015740495888582474 4 0.0 5 3.1480991777164946E-4 6 0.0 7 0.0 8 0.0 9 3.1480991777164946E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014166446299724226 26-27 0.008421165300391624 28-29 0.0 30-31 0.0 32-33 2.3610743832873712E-4 34-35 0.010073917368692783 36-37 0.001023132232757861 38-39 0.012356289272537244 40-41 0.012434991751980153 42-43 0.005115661163789304 44-45 4.7221487665747425E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 8.65727273872036E-4 68-69 0.0013379421505295102 70-71 0.0 72-73 3.9351239721456186E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.5740495888582473E-4 82-83 0.0011805371916436857 84-85 0.005351768602118041 86-87 0.004092528931031444 88-89 0.0 90-91 0.0012592396710865979 92-93 7.083223149862113E-4 94-95 0.0 96-97 7.870247944291237E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 635304.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.77844000984717 #Duplication Level Percentage of deduplicated Percentage of total 1 82.82807784647717 61.109263725272676 2 11.911713636378424 17.576552998720484 3 3.1183932244110943 6.902105623029834 4 1.0853852272898976 3.2031211551672816 5 0.4318574029628191 1.5930882748650372 6 0.21521816526399298 0.9527076296975329 7 0.11395010110855837 0.5884942489127655 8 0.06785093399731999 0.4004748850826698 9 0.04577220838232284 0.30393019172280866 >10 0.15794849844816375 2.026014366050644 >50 0.010852469782105635 0.5600026788199963 >100 0.010213995146297278 1.6352526030300993 >500 0.0017023325242187751 0.9154337488310047 >1k 0.0010639578276367345 2.2335578707972394 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4210 0.6626748769093221 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3977 0.6259995214889249 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2677 0.4213730749373528 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1884 0.2965509425408938 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1479 0.23280193419213477 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 978 0.1539420497903366 No Hit ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT 952 0.14984952085930514 RNA PCR Primer, Index 39 (95% over 23bp) CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT 932 0.14670142168158867 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 712 0.11207233072670722 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 653 0.10278543815244355 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.5740495888582473E-4 0.0 5 0.0 0.0 0.0 1.5740495888582473E-4 0.0 6 0.0 0.0 0.0 1.5740495888582473E-4 0.0 7 0.0 0.0 0.0 1.5740495888582473E-4 0.0 8 0.0 0.0 0.0 1.5740495888582473E-4 0.0 9 0.0 0.0 0.0 3.1480991777164946E-4 0.0 10-11 0.0 0.0 0.0 3.1480991777164946E-4 0.0 12-13 0.0 0.0 0.0 3.1480991777164946E-4 0.0 14-15 0.0 0.0 0.0 3.1480991777164946E-4 0.0 16-17 0.0 0.0 0.0 3.1480991777164946E-4 0.0 18-19 0.0 0.0 0.0 3.1480991777164946E-4 0.0 20-21 0.0 0.0 0.0 3.9351239721456186E-4 0.0 22-23 0.0 0.0 0.0 0.001101834712200773 0.0 24-25 0.0 0.0 0.0 0.001967561986072809 0.0 26-27 0.0 0.0 0.0 0.005745280999332603 0.0 28-29 0.0 0.0 0.0 0.0140090413408384 0.0 30-31 0.0 0.0 0.0 0.03620314054373969 0.0 32-33 0.0 0.0 0.0 0.08208668605895761 0.0 34-35 0.0 0.0 0.0 0.1703908679939053 0.0 36-37 0.0 0.0 0.0 0.2976527772530946 0.0 38-39 0.0 0.0 0.0 0.4548216287005906 0.0 40-41 0.0 0.0 0.0 0.6113608603125433 0.0 42-43 0.0 0.0 0.0 0.7789184390465037 0.0 44-45 0.0 0.0 0.0 0.9988918690894437 0.0 46-47 0.0 0.0 0.0 1.2596331834838126 0.0 48-49 0.0 0.0 0.0 1.510536687947817 0.0 50-51 0.0 0.0 0.0 1.7556949114124891 0.0 52-53 0.0 0.0 0.0 1.9980985480966593 0.0 54-55 0.0 0.0 0.0 2.294649490637553 0.0 56-57 0.0 0.0 0.0 2.645190334076285 0.0 58-59 0.0 0.0 0.0 3.0028931031443213 0.0 60-61 0.0 0.0 0.0 3.362406029239545 0.0 62-63 0.0 0.0 0.0 3.7248309470741567 0.0 64-65 0.0 0.0 0.0 4.121491443466435 0.0 66-67 0.0 0.0 0.0 4.589220278795663 0.0 68-69 0.0 0.0 0.0 5.1014160150101375 0.0 70-71 0.0 0.0 0.0 5.618570007429514 0.0 72-73 0.0 0.0 0.0 6.141075768451009 0.0 74-75 0.0 0.0 0.0 6.693252364222483 0.0 76-77 0.0 0.0 0.0 7.291469910468059 0.0 78-79 0.0 0.0 0.0 7.971852845252037 0.0 80-81 0.0 0.0 0.0 8.654675556898745 0.0 82-83 0.0 0.0 0.0 9.313021797438708 0.0 84-85 0.0 0.0 0.0 9.979159583443517 0.0 86-87 0.0 0.0 0.0 10.675204311636634 0.0 88-89 0.0 0.0 0.0 11.400368957223627 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3795 0.0 28.172302 1 TCGTATC 60 1.7113004E-4 23.749292 94-95 ACACCGC 100 0.0016531216 23.74929 6 GTAAGCG 50 0.0016540288 23.74929 94-95 GTATCAA 4970 0.0 23.424011 1 CTCCGAC 285 0.0 22.499329 94-95 GAGTACT 3590 0.0 22.161594 12-13 ATGGGTG 370 0.0 21.823671 5 CATGGGT 820 0.0 21.432287 4 GTAGGAC 255 1.189801E-8 20.489586 3 ATAGCCC 375 0.0 20.266062 3 GTACTTT 3880 0.0 20.26035 14-15 AGTACTT 3685 0.0 20.172398 12-13 GTACATG 2360 0.0 20.134441 1 TATCAAC 5880 0.0 19.794191 2 TAGCCCT 675 0.0 19.703115 4 ATCAACG 5920 0.0 19.496883 3 TACATGG 2435 0.0 19.31458 2 TCAACGC 5990 0.0 19.269041 4 AGAGTAC 4915 0.0 19.183048 10-11 >>END_MODULE