FastQCFastQC Report
Thu 26 May 2016
SRR938315_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938315_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525981
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121480.7960780638815292No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78970.5175031668153143No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68140.44653242733690657No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37370.24489164675051656No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30920.20262375481739286No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA29230.19154891181476047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25370.16625370827028646No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24000.1572758769604602No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA23140.1516401580360437No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21400.14023765695641036No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20430.13388109026259173No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG19820.12988366172318005No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18110.11867775548974725No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA17970.11776031287414457No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17180.1125833152575294No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43600.046.9060131
GTATCAA76550.041.4407161
TATCAAC98450.032.1235852
ATCAACG99550.031.7686253
TCAACGC101000.031.3125444
CAACGCA102800.030.6717455
AACGCAG106150.029.7241826
GTACATG86050.028.5197321
TACATGG85650.028.0402622
GGACCGA3350.026.9507336
ACCGTGC1955.329639E-1026.8096498
ACATGGG85850.026.7562273
ACGCAGA120750.025.9779017
GTTTACG1109.39692E-525.94211
CGCAGAG121750.025.724658
CATGGGA51850.024.1226524
GCAGAGT129100.023.8551319
TAGGTCG1401.7634173E-523.7786985
TAGGACA4950.023.0581324
CATGGGG31850.022.9947854
Produced by FastQC (version 0.11.5)