##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938315_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1525981 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.69987503120943 31.0 31.0 34.0 30.0 34.0 2 31.732465214180255 33.0 31.0 34.0 30.0 34.0 3 31.78305168937228 33.0 31.0 34.0 30.0 34.0 4 35.470226038201 37.0 35.0 37.0 33.0 37.0 5 35.467571352461135 37.0 35.0 37.0 33.0 37.0 6 35.51679477005284 37.0 35.0 37.0 33.0 37.0 7 35.45410132891563 37.0 35.0 37.0 33.0 37.0 8 35.43808409147952 37.0 35.0 37.0 33.0 37.0 9 37.021303017534294 39.0 37.0 39.0 33.0 39.0 10-11 36.8803726914031 39.0 37.0 39.0 33.0 39.0 12-13 36.766723832079165 39.0 37.0 39.0 33.0 39.0 14-15 37.817176622775776 40.0 37.5 41.0 33.0 41.0 16-17 37.68287940675539 40.0 37.0 41.0 32.5 41.0 18-19 37.85177928165554 40.0 37.5 41.0 33.0 41.0 20-21 37.8516872097359 40.0 37.5 41.0 33.0 41.0 22-23 37.76957707861369 40.0 37.0 41.0 33.0 41.0 24-25 37.64980134090791 40.0 37.0 41.0 32.0 41.0 26-27 37.4483404446058 40.0 37.0 41.0 32.0 41.0 28-29 37.25675516274449 39.0 36.0 41.0 31.5 41.0 30-31 36.97567204309883 39.0 36.0 40.5 31.0 41.0 32-33 36.67221217040055 39.0 35.0 40.0 30.5 41.0 34-35 36.422494120175806 38.5 35.0 40.0 30.0 41.0 36-37 36.143578131051434 38.0 35.0 40.0 30.0 41.0 38-39 35.98663712064567 38.0 35.0 40.0 30.0 41.0 40-41 35.8689954199954 38.0 35.0 40.0 29.5 41.0 42-43 35.55389877069243 38.0 35.0 40.0 27.5 41.0 44-45 35.180213908298995 38.0 34.5 40.0 26.5 41.0 46-47 34.736877457845154 38.0 34.0 40.0 24.5 41.0 48-49 34.328745246500446 38.0 33.5 40.0 23.5 41.0 50-51 33.613287452465 37.5 32.5 39.5 23.0 40.5 52-53 33.637147513632215 37.5 33.0 39.5 23.0 40.5 54-55 33.77133594717104 38.0 33.0 40.0 22.5 41.0 56-57 33.48046666373959 37.0 33.0 40.0 20.5 41.0 58-59 33.132938417975055 37.0 32.5 40.0 19.5 41.0 60-61 32.69492772190479 36.0 32.0 39.0 17.5 41.0 62-63 32.17339141181968 35.5 31.0 39.0 13.5 40.0 64-65 31.693259286976705 35.0 31.0 39.0 8.5 40.0 66-67 31.207606778852423 35.0 30.0 38.0 7.0 40.0 68-69 30.673368475754287 35.0 29.5 38.0 2.0 40.0 70-71 30.080920732302694 34.5 29.0 37.0 2.0 39.5 72-73 29.485463777071928 34.0 28.5 36.5 2.0 39.0 74-75 28.85909326525035 34.0 27.5 36.0 2.0 39.0 76-77 28.156923644527684 33.5 26.0 35.0 2.0 38.0 78-79 27.423014113544006 33.0 25.5 35.0 2.0 37.0 80-81 26.775333048052367 32.5 24.5 35.0 2.0 36.5 82-83 26.07827292738245 32.0 22.5 35.0 2.0 36.0 84-85 25.4191320206477 31.5 20.0 34.5 2.0 35.5 86-87 24.697645317995438 31.0 17.5 34.0 2.0 35.0 88-89 23.911589659373217 31.0 9.5 34.0 2.0 35.0 90-91 23.03055083909957 30.0 3.5 34.0 2.0 35.0 92-93 22.116845491523158 29.0 2.0 34.0 2.0 35.0 94-95 21.13497284697516 29.0 2.0 34.0 2.0 35.0 96-97 19.88193430979809 27.5 2.0 33.5 2.0 35.0 98-99 18.36218242560032 25.5 2.0 33.0 2.0 35.0 100-101 14.567911068355373 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2665728318663696 1101 2 0.05649412651820285 1101 3 0.07875614748670756 1101 4 0.15981013231137098 1101 5 0.015109704583281314 1101 6 0.31781796435065957 1101 7 0.28412865575561597 1101 8 0.22446476878171495 1101 9 0.3026729940896331 1101 10-11 0.49542621027369904 1101 12-13 0.38908241510900865 1101 14-15 0.51937585679552 1101 16-17 0.4398992345188759 1101 18-19 0.487797863054638 1101 20-21 0.4719493395555787 1101 22-23 0.41109658403060934 1101 24-25 0.4113995814128586 1101 26-27 0.34637908419955465 1101 28-29 0.3593640240986673 1101 30-31 0.31129308818122325 1101 32-33 0.4540314677440307 1101 34-35 0.47281324558900906 1101 36-37 0.5129677727050392 1101 38-39 0.5803225413502062 1101 40-41 0.4943415469180863 1101 42-43 0.5966149704654384 1101 44-45 0.6321764151737241 1101 46-47 0.6721237943572547 1101 48-49 0.5711802670822692 1101 50-51 0.5120207789458391 1101 52-53 0.36633565399785084 1101 54-55 0.4364519702032439 1101 56-57 0.5229403523273319 1101 58-59 0.48745845870206495 1101 60-61 0.5387089076452867 1101 62-63 0.5227011229105116 1101 64-65 0.5246946351332546 1101 66-67 0.5825494494398988 1101 68-69 0.37757970502013904 1101 70-71 0.43145094074987256 1101 72-73 0.5401363768292633 1101 74-75 0.3371049679053506 1101 76-77 0.17712652851721344 1101 78-79 0.462370562159812 1101 80-81 0.5436555371042537 1101 82-83 0.31742944683749386 1101 84-85 0.2313407309400759 1101 86-87 0.5624285120978385 1101 88-89 0.49582622627320916 1101 90-91 0.4318856885747593 1101 92-93 0.18930789940122494 1101 94-95 0.3696434231446233 1101 96-97 0.4353706884119184 1101 98-99 0.9660655469291761 1101 100-101 0.9973869588401332 1102 1 0.2619342430865004 1102 2 0.164994478221125 1102 3 0.25063757984143464 1102 4 0.11629621492427589 1102 5 -0.001179651297938733 1102 6 0.2389133325690338 1102 7 0.09707618556932829 1102 8 0.12113218099374734 1102 9 0.2671274979940179 1102 10-11 0.37443837887067843 1102 12-13 0.3102612519934098 1102 14-15 0.4482261123879283 1102 16-17 0.44895598997027264 1102 18-19 0.3477994351829139 1102 20-21 0.36669818801212983 1102 22-23 0.4116703298126083 1102 24-25 0.4280114667025927 1102 26-27 0.23679971765379548 1102 28-29 0.21500820771028373 1102 30-31 0.17564151394597616 1102 32-33 0.40754468820828293 1102 34-35 0.21910213145249458 1102 36-37 0.3014715441921396 1102 38-39 0.45863170928917185 1102 40-41 0.540493236226645 1102 42-43 0.5951303627357518 1102 44-45 0.7116025235241565 1102 46-47 0.7115185735947946 1102 48-49 0.6883354280390392 1102 50-51 0.9099941111122334 1102 52-53 0.6392409146307898 1102 54-55 0.4723378239661429 1102 56-57 0.38701244008127134 1102 58-59 0.6012623121991538 1102 60-61 0.5268903928364921 1102 62-63 0.43578836144288857 1102 64-65 0.6703113956413951 1102 66-67 0.33717831371498264 1102 68-69 0.47050383119234596 1102 70-71 0.5893513715454368 1102 72-73 0.5302664096656748 1102 74-75 0.6341355093333654 1102 76-77 0.6684352523704504 1102 78-79 0.5560260521289884 1102 80-81 0.8877287755912526 1102 82-83 0.8740694197385395 1102 84-85 0.7539011768410369 1102 86-87 0.9275646227497383 1102 88-89 0.7945488315328681 1102 90-91 0.7944367017060827 1102 92-93 1.0063851022446677 1102 94-95 1.05639281293195 1102 96-97 1.2199421132787727 1102 98-99 1.1890050864414619 1102 100-101 1.3827642210030557 1103 1 0.1292766116978541 1103 2 0.1743773267441462 1103 3 0.13060134542184443 1103 4 0.12493338086392214 1103 5 0.1546143568042666 1103 6 0.14894155809330556 1103 7 0.23263257052504116 1103 8 0.19335686549769804 1103 9 0.23317289210164915 1103 10-11 0.3529792047977267 1103 12-13 0.40573156076354167 1103 14-15 0.48492784713020143 1103 16-17 0.43026921553342135 1103 18-19 0.39526691362498667 1103 20-21 0.2883517527481132 1103 22-23 0.3801918721858826 1103 24-25 0.45934413133205254 1103 26-27 0.4086721662763466 1103 28-29 0.5979543148428661 1103 30-31 0.6439468939019619 1103 32-33 0.49489452142108803 1103 34-35 0.5299339679777191 1103 36-37 0.4300182018390828 1103 38-39 0.2425987561839733 1103 40-41 0.4829745941620587 1103 42-43 0.5227929316472384 1103 44-45 0.6501023718994432 1103 46-47 0.5616017727985678 1103 48-49 0.27904510947161043 1103 50-51 0.43858197957973033 1103 52-53 0.4491812755753699 1103 54-55 0.44462199201298347 1103 56-57 0.46888162294067826 1103 58-59 0.47192137430307923 1103 60-61 0.6006775894898624 1103 62-63 0.6183281920897414 1103 64-65 0.6358556506197424 1103 66-67 0.7058223222059716 1103 68-69 0.7166183506197505 1103 70-71 0.7695760093956316 1103 72-73 0.7864581410644327 1103 74-75 0.7426762575041472 1103 76-77 0.836014388809371 1103 78-79 0.9397602190094858 1103 80-81 0.8480174846039787 1103 82-83 0.7524399594447786 1103 84-85 1.041008799287713 1103 86-87 1.244163641465672 1103 88-89 1.2684538289280347 1103 90-91 0.9077823982855868 1103 92-93 1.1364137943065344 1103 94-95 1.1648044857024829 1103 96-97 1.3134401343818247 1103 98-99 1.2970781306770007 1103 100-101 1.498669653833307 1104 1 0.35959710130051903 1104 2 0.29698933754288603 1104 3 0.25500530496016793 1104 4 0.23291108138929673 1104 5 0.24832143909052462 1104 6 0.37688199325418026 1104 7 0.2711152204733267 1104 8 0.3844662008822439 1104 9 0.40365670705932644 1104 10-11 0.4687943939810637 1104 12-13 0.454141808365371 1104 14-15 0.5314761334088587 1104 16-17 0.6569904982386348 1104 18-19 0.5395766642308288 1104 20-21 0.5641231047191013 1104 22-23 0.5207620173266392 1104 24-25 0.5854005745434492 1104 26-27 0.6527705722298407 1104 28-29 0.7599733283935706 1104 30-31 0.7862910645609062 1104 32-33 0.5954480875820494 1104 34-35 0.6785938337776045 1104 36-37 0.7948761789795071 1104 38-39 0.8082713063969962 1104 40-41 0.6173683225281721 1104 42-43 0.657128222843582 1104 44-45 0.6308468577083559 1104 46-47 0.6273247320717772 1104 48-49 0.6491211711424327 1104 50-51 0.6388424263907027 1104 52-53 0.7647755983949907 1104 54-55 0.8863086044867998 1104 56-57 0.8770814967313285 1104 58-59 0.8747515629823255 1104 60-61 0.9434858969029847 1104 62-63 0.9444158142383614 1104 64-65 1.0461004519520323 1104 66-67 1.083746887847898 1104 68-69 0.9678156591493341 1104 70-71 1.098074864923266 1104 72-73 1.0628991867350415 1104 74-75 1.043387958753918 1104 76-77 1.0618116921113518 1104 78-79 1.126238081964054 1104 80-81 1.14759684900174 1104 82-83 1.2287133483152068 1104 84-85 1.4540574645678213 1104 86-87 1.495547276817728 1104 88-89 1.21806157175968 1104 90-91 1.3428644903736036 1104 92-93 1.283581365224613 1104 94-95 1.4345965763342399 1104 96-97 1.7245082836971939 1104 98-99 1.9433105201784748 1104 100-101 1.7984868743150688 1105 1 0.14690842568675322 1105 2 -0.09821444156192882 1105 3 -0.06215602313021762 1105 4 -0.057564386241516274 1105 5 -0.03658291393968938 1105 6 -0.016962507971094 1105 7 -0.0961520027358631 1105 8 -0.06638880415103898 1105 9 0.23123722874893815 1105 10-11 0.12886870214021684 1105 12-13 0.19633876166547992 1105 14-15 0.27880642159173874 1105 16-17 0.27601646651077516 1105 18-19 0.14242784309480072 1105 20-21 0.10936548687991632 1105 22-23 0.13767353316659836 1105 24-25 0.22297979614895524 1105 26-27 0.3151559796210037 1105 28-29 0.1828438414692144 1105 30-31 0.2400129729391196 1105 32-33 0.3064484696179335 1105 34-35 0.3995142570094501 1105 36-37 0.42082672930002474 1105 38-39 0.4384818110765707 1105 40-41 0.4410115503973273 1105 42-43 0.4896688559191702 1105 44-45 0.5576986059946378 1105 46-47 0.30894938689581863 1105 48-49 0.19108883027926282 1105 50-51 0.3995597677695528 1105 52-53 0.2709633450659794 1105 54-55 0.14947286394001225 1105 56-57 0.24343321378653826 1105 58-59 0.3315275795536792 1105 60-61 0.33513078655561657 1105 62-63 0.5136398456030733 1105 64-65 0.4723473706074408 1105 66-67 0.4980833292780211 1105 68-69 0.43460486540078236 1105 70-71 0.5020565722314814 1105 72-73 0.459230371177032 1105 74-75 0.47177892672827326 1105 76-77 0.5854089449392177 1105 78-79 0.6158949687056605 1105 80-81 0.6505055148249248 1105 82-83 0.7440541591900107 1105 84-85 0.6467960227382328 1105 86-87 0.7697544434042634 1105 88-89 0.8299993580142306 1105 90-91 0.5714107421367061 1105 92-93 0.5450851122267935 1105 94-95 0.5244908555283914 1105 96-97 0.5397439983875678 1105 98-99 0.8019180211553518 1105 100-101 0.8771617545112278 1106 1 0.24788789478822437 1106 2 0.05728759109190662 1106 3 -0.10576757823812599 1106 4 -0.012020897667433417 1106 5 0.07260088613767834 1106 6 -0.0824143264116799 1106 7 0.15350459919242354 1106 8 0.2116567097782962 1106 9 0.013531609574116032 1106 10-11 0.05967982955148443 1106 12-13 0.07744128478371692 1106 14-15 0.2984726211494504 1106 16-17 0.13462185432069163 1106 18-19 0.034951910154127575 1106 20-21 0.06080348911328315 1106 22-23 0.2520000934200013 1106 24-25 0.04445604292845218 1106 26-27 0.10242744291280559 1106 28-29 0.22293602250313427 1106 30-31 0.03297805654431585 1106 32-33 0.25777608086649906 1106 34-35 -0.25601094862796714 1106 36-37 0.12530440660071207 1106 38-39 0.22669661551118736 1106 40-41 0.4177685004677514 1106 42-43 0.18358165981369012 1106 44-45 0.2049043455959918 1106 46-47 0.2546581882504384 1106 48-49 0.4512951610653957 1106 50-51 0.24501056440929148 1106 52-53 0.26711152078652844 1106 54-55 0.4235895535971963 1106 56-57 -0.22411475611412612 1106 58-59 0.04944431008290451 1106 60-61 0.46137720112941594 1106 62-63 0.6233768778554847 1106 64-65 0.43950942822241146 1106 66-67 0.0793650324740689 1106 68-69 0.14374542140641466 1106 70-71 -0.0640340542054254 1106 72-73 0.2989475877915133 1106 74-75 0.5982324384388384 1106 76-77 0.7182032783236387 1106 78-79 0.7746892072085814 1106 80-81 0.7815881493194645 1106 82-83 0.4820135134118715 1106 84-85 0.47014052289515007 1106 86-87 0.28060761062197415 1106 88-89 0.1869919426594855 1106 90-91 0.30228719181666364 1106 92-93 0.4379184565079939 1106 94-95 0.46324447744747843 1106 96-97 0.7729990544771326 1106 98-99 0.8631472475791213 1106 100-101 0.590130414419411 1107 1 0.2348785848863102 1107 2 0.0415760093247286 1107 3 0.14748997128428698 1107 4 0.07234026276300654 1107 5 -0.03239450563494728 1107 6 0.12408739686380699 1107 7 0.16653369790376615 1107 8 0.06751978587543306 1107 9 0.2890834516740739 1107 10-11 0.34570123444701295 1107 12-13 0.3162253357362417 1107 14-15 0.2733859076859133 1107 16-17 0.3922279947149647 1107 18-19 0.18304567979406983 1107 20-21 0.27353868735867337 1107 22-23 0.26118063515698253 1107 24-25 0.2824910507981713 1107 26-27 0.19546727888556603 1107 28-29 0.37152634467594225 1107 30-31 0.2078261133015573 1107 32-33 0.38382713233023225 1107 34-35 0.29970297847957994 1107 36-37 0.17350829730052197 1107 38-39 0.4419396788921759 1107 40-41 0.3933560723625149 1107 42-43 0.34522471733133386 1107 44-45 0.36974860949808885 1107 46-47 0.3018345266977107 1107 48-49 0.3555010159877696 1107 50-51 0.37961477905923147 1107 52-53 0.42914797250641357 1107 54-55 0.4458642405950357 1107 56-57 0.45241498298508276 1107 58-59 0.35301783925215346 1107 60-61 0.31080469987887227 1107 62-63 0.4072951720601168 1107 64-65 0.32824428199593214 1107 66-67 0.2584227919777362 1107 68-69 0.27183197179550334 1107 70-71 0.5093428511545888 1107 72-73 0.29699014472556584 1107 74-75 0.2631723482531747 1107 76-77 0.49746130790790843 1107 78-79 0.5809521821629104 1107 80-81 0.739645218047098 1107 82-83 0.5202958813994414 1107 84-85 0.3597127364750392 1107 86-87 0.5668538383801724 1107 88-89 0.4614535679437175 1107 90-91 0.5959307697067011 1107 92-93 0.5440764408345231 1107 94-95 0.6473121038713998 1107 96-97 0.8539763284953459 1107 98-99 1.1097081174072336 1107 100-101 0.8331807861008933 1108 1 0.12042604449170113 1108 2 -0.058988147630735455 1108 3 -0.0162633664036278 1108 4 -0.15416892368717328 1108 5 -0.06867470896705896 1108 6 0.029693812127902675 1108 7 0.14012783580388088 1108 8 0.11591565095048395 1108 9 0.12568953565493501 1108 10-11 0.2304955218185185 1108 12-13 0.08730974992957385 1108 14-15 0.21626056069204935 1108 16-17 0.2762496005344417 1108 18-19 0.36011300157363024 1108 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0.34235809836234665 2201 9 0.33822470177083375 2201 10-11 0.39856914520927234 2201 12-13 0.5613304541207427 2201 14-15 0.6656239753481259 2201 16-17 0.7375533908350391 2201 18-19 0.6274043193255139 2201 20-21 0.5416126063601112 2201 22-23 0.6974850004331543 2201 24-25 0.6014655391460693 2201 26-27 0.5908701144373225 2201 28-29 0.8519009494159064 2201 30-31 0.7353667430228157 2201 32-33 0.8948438859087204 2201 34-35 0.788924706815898 2201 36-37 0.900568881382334 2201 38-39 1.0742973207772692 2201 40-41 1.0819336307663932 2201 42-43 1.2507070420766908 2201 44-45 1.1095822204332109 2201 46-47 1.3915280944588275 2201 48-49 1.3064866217855808 2201 50-51 1.3534086446808615 2201 52-53 1.2320484931172757 2201 54-55 1.1587610415330474 2201 56-57 0.9950704567094277 2201 58-59 1.1907610872217447 2201 60-61 1.176603705672676 2201 62-63 1.265779537966985 2201 64-65 1.130282075018723 2201 66-67 1.420676139683291 2201 68-69 1.3949456174762354 2201 70-71 1.3373388003663642 2201 72-73 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3.2451445854886103 2202 100-101 3.0890721784393893 2203 1 0.5312044826689082 2203 2 0.4942922962895828 2203 3 0.6365270429028733 2203 4 0.5121589869261953 2203 5 0.37881866486710436 2203 6 0.4159742669153985 2203 7 0.5027290925738512 2203 8 0.4960175550667856 2203 9 0.662777515173687 2203 10-11 0.6395951689351449 2203 12-13 0.5654037749697878 2203 14-15 0.6646973176724345 2203 16-17 0.7575318296691833 2203 18-19 0.7701063800919243 2203 20-21 0.7755213694134895 2203 22-23 0.612766870441952 2203 24-25 0.7458165423272689 2203 26-27 0.7997415821141871 2203 28-29 0.8408805593678323 2203 30-31 0.7857427033250772 2203 32-33 0.8167694870013733 2203 34-35 0.7833370218780757 2203 36-37 0.810510210958931 2203 38-39 0.9193192828147332 2203 40-41 0.9250942242281823 2203 42-43 1.0207549176003283 2203 44-45 1.0343214391344446 2203 46-47 1.186621139622737 2203 48-49 1.178480442636058 2203 50-51 1.4054210201601123 2203 52-53 1.1002828899057207 2203 54-55 0.9908327030599651 2203 56-57 1.0946730927265946 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0.7573254144770303 2204 18-19 0.7004841892320144 2204 20-21 0.7831592648959287 2204 22-23 0.7308800969087557 2204 24-25 0.6342547313779789 2204 26-27 0.6278478640698779 2204 28-29 0.5991176989546432 2204 30-31 0.7157823542433803 2204 32-33 0.7820010613785868 2204 34-35 0.720123302681678 2204 36-37 0.7748222509917397 2204 38-39 0.8637514596896168 2204 40-41 0.9445985930830645 2204 42-43 0.913363013556193 2204 44-45 0.8840138431620446 2204 46-47 0.8938422577637013 2204 48-49 1.0149724084447485 2204 50-51 1.035500460673596 2204 52-53 0.9857523271229667 2204 54-55 0.9987436853082627 2204 56-57 1.0531239013727784 2204 58-59 1.109087149704152 2204 60-61 1.293003774472126 2204 62-63 1.3240909805663534 2204 64-65 1.4872065832590202 2204 66-67 1.6662315388764632 2204 68-69 1.7427854711210493 2204 70-71 1.7696377965198486 2204 72-73 1.835807280236743 2204 74-75 1.8765111783473678 2204 76-77 1.7724467382875524 2204 78-79 1.8816819626093206 2204 80-81 2.138161982633715 2204 82-83 2.326022409714348 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0.8758553220292455 2205 44-45 0.9255079130211996 2205 46-47 1.0564707406245475 2205 48-49 0.8352983388816284 2205 50-51 0.7995667040155396 2205 52-53 0.9218613765876782 2205 54-55 0.9250810594685319 2205 56-57 1.0454239163857935 2205 58-59 1.1980186459519544 2205 60-61 1.221929836431471 2205 62-63 1.0949305252702501 2205 64-65 1.3226338092556311 2205 66-67 1.3777168123776455 2205 68-69 1.3883567374794161 2205 70-71 1.5846605380655134 2205 72-73 1.6864621437134595 2205 74-75 1.5147075654970727 2205 76-77 1.5040113904613719 2205 78-79 1.5799853862110496 2205 80-81 1.7016628062350243 2205 82-83 1.6697892597089563 2205 84-85 1.946738409429699 2205 86-87 1.9968694322071805 2205 88-89 1.9889579443789884 2205 90-91 2.0182638805756845 2205 92-93 2.339173802748487 2205 94-95 2.7373033758770724 2205 96-97 2.7627538620124774 2205 98-99 3.008025739640022 2205 100-101 2.5875957794873745 2206 1 0.16765542625737595 2206 2 0.16187888216814628 2206 3 0.19616859441411805 2206 4 0.15059542587883357 2206 5 0.03344362215394625 2206 6 -0.37351687909207953 2206 7 0.02305945428491185 2206 8 0.09430821937645817 2206 9 -0.06328187239773087 2206 10-11 0.007328735908096462 2206 12-13 0.2108654026419572 2206 14-15 0.2755317664216079 2206 16-17 -0.08895016900886077 2206 18-19 0.01829642708835877 2206 20-21 0.35032154170063734 2206 22-23 0.3718059686636508 2206 24-25 0.1168150684702951 2206 26-27 0.17820263561846872 2206 28-29 0.3766838445203575 2206 30-31 0.12290228999776076 2206 32-33 0.4072566542938887 2206 34-35 0.349670884505187 2206 36-37 0.12339511218255694 2206 38-39 0.4955797027113178 2206 40-41 0.6117190182026917 2206 42-43 0.61103279499617 2206 44-45 0.4066108315206307 2206 46-47 0.6619777140657561 2206 48-49 0.6802268546384909 2206 50-51 0.7308337056290313 2206 52-53 0.5525066778174477 2206 54-55 0.6247282133319061 2206 56-57 0.2179470487874866 2206 58-59 0.3383319459072638 2206 60-61 0.794134062127597 2206 62-63 0.5926657700023412 2206 64-65 0.5697312770314049 2206 66-67 0.8054256419521231 2206 68-69 0.5389836509014252 2206 70-71 0.4634121940254019 2206 72-73 0.7545883517348315 2206 74-75 1.1573040304009332 2206 76-77 1.0549896189178547 2206 78-79 1.0613549716687878 2206 80-81 1.3526684851658182 2206 82-83 1.1652785234019305 2206 84-85 1.4301432671064624 2206 86-87 1.6431485623370108 2206 88-89 1.3065079840782765 2206 90-91 1.4380164471768033 2206 92-93 1.3921752733356492 2206 94-95 1.774595738374984 2206 96-97 2.3908050387373905 2206 98-99 2.174943033147205 2206 100-101 1.9746850157663314 2207 1 0.28924695379912535 2207 2 0.2974864508006938 2207 3 0.2741296738923147 2207 4 0.21960642286941834 2207 5 0.24507020832247406 2207 6 0.17911162876193742 2207 7 0.3130602063615484 2207 8 0.2790334316899177 2207 9 0.1774415586425846 2207 10-11 0.2809129145866862 2207 12-13 0.27892222555162505 2207 14-15 0.5235927609484889 2207 16-17 0.36624032681711327 2207 18-19 0.3353108087942971 2207 20-21 0.34703759720315475 2207 22-23 0.24761644238672176 2207 24-25 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5 1128.0 6 1278.0 7 1558.0 8 2002.0 9 2613.0 10 3467.0 11 4921.0 12 6898.0 13 8990.0 14 10658.0 15 13222.0 16 16606.0 17 20369.0 18 18173.0 19 14928.0 20 15232.0 21 16157.0 22 18113.0 23 21023.0 24 23723.0 25 26833.0 26 31206.0 27 36185.0 28 42494.0 29 50566.0 30 60188.0 31 71975.0 32 87756.0 33 108304.0 34 136753.0 35 172519.0 36 194961.0 37 188015.0 38 86776.0 39 4583.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.23115619714091 18.275570771617296 13.519146088829148 22.97412694241264 2 15.608110014058111 20.201694189205234 39.96852505302132 24.221670743715343 3 19.229136665868467 25.39997966232906 29.21184964556688 26.159034026235595 4 12.470435407310697 15.88946368762732 36.41898563513438 35.2211152699276 5 13.286497569607159 38.118181543716226 33.5374522027117 15.05786868396492 6 32.28900616416702 35.71005983138071 17.226741387022848 14.774192617429415 7 28.745919584682543 33.49047575348392 20.175605343541474 17.58799931829206 8 27.447015337743014 33.870985714969294 19.606733950234105 19.075264997053583 9 27.721185529135155 15.91754493714404 19.830483255780724 36.53078627794008 10-11 25.146707622305524 26.712310130783766 26.821933121452375 21.319049125458335 12-13 26.408009520156572 24.209849952628698 27.572982949609393 21.80915757760534 14-15 22.77483916661942 25.219237799392292 26.50924785793109 25.496675176057202 16-17 21.661748105884314 28.721761976619025 27.245046587576383 22.371443329920275 18-19 21.797719221420124 26.385268813538875 30.53432312166163 21.282688843379372 20-21 22.274261416631013 25.714257613682744 30.748607088236596 21.262873881449647 22-23 21.769344047532815 25.910209309711576 30.713703475122657 21.606743167632953 24-25 21.480964996993972 26.149977479382326 30.930576835954838 21.438480687668864 26-27 21.11382525085394 26.521458186967035 30.61219605669117 21.752520505487855 28-29 21.18074655802173 26.885753006301417 30.60320099553308 21.330299440143772 30-31 21.15871941193539 26.527694216894727 30.44696648038282 21.866619890787064 32-33 20.80572312360392 26.619946487496154 30.45203707455363 22.122293314346294 34-35 21.67569405307192 26.75194735494464 30.20251704581034 21.369841546173106 36-37 21.90652029100293 26.30224861133695 30.12667780394543 21.664553293714693 38-39 21.28841882248212 26.589546874790027 30.257937450897426 21.864096851830425 40-41 21.605495084683984 26.59760881479131 30.28773412978322 21.50916197074148 42-43 21.378791578512654 26.65842894390537 29.93220266764558 22.0305768099364 44-45 21.286366934033087 26.972594668880923 30.153138764679323 21.58789963240667 46-47 21.811379394190432 27.294364307609225 29.23417527803575 21.660081020164597 48-49 21.527942303615134 27.509626604720843 28.981495226101917 21.980935865562103 50-51 21.78742080742187 27.70182106065462 28.872006705687053 21.63875142623646 52-53 22.08417372155856 27.331997450572064 28.882822983518835 21.701005844350544 54-55 21.258072719026778 27.66135934954234 29.18277278155062 21.89779514988026 56-57 21.581049324882525 27.89945938825047 28.707345759178228 21.812145527688777 58-59 21.486956806711675 27.259487448384352 28.945697057088548 22.30785868781543 60-61 21.126151252506357 27.63556610717865 29.286041552915176 21.95224108739982 62-63 21.347020404444955 27.52861658931479 29.36150049203067 21.762862514209576 64-65 20.9847775352151 27.591643144057983 29.24555506256566 22.17802425816126 66-67 20.967003050127207 27.717031559051485 29.145099283148014 22.17086610767329 68-69 21.656072871243524 27.23217665056925 29.27085828585323 21.840892192333996 70-71 21.307924359105463 27.523428015554003 28.840221458331694 22.328426167008843 72-73 21.300444976699108 27.58927637946218 28.876421544384108 22.233857099454603 74-75 21.194909393630514 27.953466115610386 28.680840743204932 22.170783747554168 76-77 21.37210568282633 27.55537756109647 28.849653637275622 22.222863118801577 78-79 21.463482726350474 27.738566152087806 28.678891519016876 22.119059602544848 80-81 21.508045992076884 28.0253159579662 28.31586294125164 22.15077510870527 82-83 21.5014516118462 27.940316798720747 28.287851680003147 22.27037990942991 84-85 21.406724922767197 27.905060993272198 28.329792267675728 22.35842181628488 86-87 21.573325101553685 27.902783730962373 28.13731735738901 22.38657381009494 88-89 21.45275413888644 27.875095751030603 27.97359189034249 22.698558219740463 90-91 21.416844290625683 27.94844573163317 28.043957873704368 22.59075210403678 92-93 21.673771705546912 27.475245983361525 28.211729393885065 22.639252917206495 94-95 21.43203446685437 27.25810115640191 28.475933944429638 22.833930432314087 96-97 21.84782580191747 26.8158199068538 28.318202320263495 23.01815197096524 98-99 21.578567537858603 27.0626653845574 27.902492837296005 23.45627424028799 100-101 22.115216886637665 26.482131919643148 28.288613402339042 23.114037791380145 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2813.0 1 1923.0 2 2059.0 3 2686.0 4 1837.0 5 1570.5 6 2595.5 7 4768.5 8 6838.5 9 6845.0 10 4941.5 11 3425.5 12 3082.5 13 3018.0 14 2959.0 15 2906.5 16 2897.5 17 3044.0 18 3209.5 19 3406.5 20 3702.0 21 4181.0 22 4895.0 23 5833.5 24 6948.0 25 8273.0 26 10096.5 27 12374.0 28 14291.5 29 16086.5 30 18403.5 31 21795.0 32 26869.5 33 28777.0 34 27795.5 35 29084.0 36 32148.5 37 35277.0 38 37767.0 39 39271.0 40 40566.5 41 43257.5 42 45555.5 43 47453.5 44 49136.0 45 52004.0 46 54030.5 47 54385.5 48 55352.0 49 54251.0 50 52836.5 51 51170.5 52 49336.0 53 49786.5 54 49872.0 55 46826.0 56 42724.5 57 38894.5 58 36260.5 59 32531.5 60 26391.0 61 22032.5 62 19304.0 63 15127.5 64 10857.0 65 8241.5 66 6326.0 67 5240.0 68 4251.5 69 3097.5 70 2277.0 71 1652.5 72 1298.5 73 1128.0 74 868.0 75 653.0 76 502.5 77 367.0 78 262.0 79 186.5 80 136.0 81 90.5 82 53.0 83 33.0 84 22.0 85 15.5 86 14.0 87 10.5 88 7.5 89 3.5 90 2.0 91 2.5 92 3.0 93 1.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007077414463220709 2 0.10445739494790564 3 0.11245225202672904 4 0.11638414895074053 5 0.15458908072905234 6 0.08931959179046135 7 0.02627817777547689 8 0.026081582929276318 9 0.022936065390067113 10-11 0.04937807220404448 12-13 0.052163165858552636 14-15 0.0269662597371789 16-17 0.030603264391889543 18-19 0.03519047746990297 20-21 0.05560357566706269 22-23 0.030570498584189452 24-25 0.04665851016493652 26-27 0.05507932274386116 28-29 0.05032828062734727 30-31 0.06412268566908763 32-33 0.04783807924213997 34-35 0.05894568805247248 36-37 0.050655938704348216 38-39 0.031160283122791177 40-41 0.0357147303931045 42-43 0.03135687796899175 44-45 0.007208477694021092 46-47 0.01231994369523605 48-49 0.008617407425125215 50-51 0.007339540924821476 52-53 0.009731444886928474 54-55 0.010681653310231253 56-57 0.01372887342634017 58-59 0.01841438392745388 60-61 0.023591381544069027 62-63 0.010583355887130967 64-65 0.009436552617627611 66-67 0.00937102100222742 68-69 0.010911013964131924 70-71 0.02994794823788763 72-73 0.025917753890775833 74-75 0.027916468160481683 76-77 0.02231351504376529 78-79 0.004783807924213997 80-81 0.004325086616412656 82-83 0.005963377001417449 84-85 0.004914871155014381 86-87 0.012254412079835856 88-89 0.035419838123803636 90-91 0.034109205815799806 92-93 0.027556044275780626 94-95 0.04518404881843221 96-97 0.04174363900992214 98-99 0.024246697698070946 100-101 0.07097073947840767 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1525981.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.35528583184076 #Duplication Level Percentage of deduplicated Percentage of total 1 76.50046382207707 40.81704113495316 2 13.13632282366601 14.01784518072067 3 4.357035759091128 6.974126649175753 4 2.008586446840483 4.286748159565415 5 1.0710294522564776 2.857254127973211 6 0.6413722278089294 2.053235912360995 7 0.4368073160480891 1.63141854408295 8 0.28146579033615693 1.201415015621649 9 0.2307501459022774 1.1080565991319475 >10 1.206513888444329 11.805229880426719 >50 0.08056056182010131 2.9299275199244144 >100 0.041709673747304256 4.1574521095867585 >500 0.004060150578943011 1.5866542593720616 >1k 0.0029528367846858258 2.8104196173867066 >5k 2.4606973205715217E-4 0.965712486951692 >10k+ 1.2303486602857608E-4 0.7974628027659 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12148 0.7960780638815292 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7897 0.5175031668153143 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6814 0.44653242733690657 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3737 0.24489164675051656 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3092 0.20262375481739286 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2923 0.19154891181476047 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2537 0.16625370827028646 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2400 0.1572758769604602 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2314 0.1516401580360437 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2140 0.14023765695641036 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2043 0.13388109026259173 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 1982 0.12988366172318005 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1811 0.11867775548974725 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 1797 0.11776031287414457 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1718 0.1125833152575294 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.553161540019175E-5 2 0.0 0.0 0.0 0.0 6.553161540019175E-5 3 0.0 0.0 0.0 0.0 6.553161540019175E-5 4 0.0 0.0 0.0 0.0 6.553161540019175E-5 5 0.0 0.0 0.0 0.0 6.553161540019175E-5 6 0.0 0.0 0.0 0.0 1.310632308003835E-4 7 0.0 0.0 0.0 0.0 1.310632308003835E-4 8 0.0 0.0 0.0 0.0 1.310632308003835E-4 9 0.0 0.0 0.0 0.0 1.310632308003835E-4 10-11 0.0 0.0 0.0 0.0 1.310632308003835E-4 12-13 0.0 0.0 0.0 0.0 1.310632308003835E-4 14-15 0.0 0.0 0.0 0.0 1.310632308003835E-4 16-17 0.0 0.0 0.0 0.0 1.310632308003835E-4 18-19 0.0 0.0 0.0 6.553161540019175E-5 1.9659484620057524E-4 20-21 0.0 0.0 0.0 9.829742310028762E-5 1.9659484620057524E-4 22-23 0.0 0.0 0.0 5.570187309016299E-4 1.9659484620057524E-4 24-25 0.0 0.0 0.0 0.0015727587696046019 1.9659484620057524E-4 26-27 0.0 0.0 0.0 0.0029489226930086284 1.9659484620057524E-4 28-29 0.0 0.0 0.0 0.005373592462815723 1.9659484620057524E-4 30-31 0.0 0.0 0.0 0.015006739926643909 1.9659484620057524E-4 32-33 0.0 0.0 0.0 0.035419838123803636 1.9659484620057524E-4 34-35 0.0 0.0 0.0 0.06359843274588609 1.9659484620057524E-4 36-37 0.0 0.0 0.0 0.10917567125671945 1.9659484620057524E-4 38-39 0.0 0.0 0.0 0.18450426315923985 1.9659484620057524E-4 40-41 0.0 0.0 0.0 0.26930217348708796 1.9659484620057524E-4 42-43 0.0 0.0 0.0 0.35331370443013377 2.2936065390067112E-4 44-45 0.0 0.0 0.0 0.4560672773776344 2.62126461600767E-4 46-47 0.0 0.0 0.0 0.5783492717143923 2.62126461600767E-4 48-49 0.0 0.0 0.0 0.7142618420543899 3.276580770009587E-4 50-51 0.0 0.0 0.0 0.8656726394365329 3.276580770009587E-4 52-53 0.0 0.0 0.0 1.0051566828158411 3.604238847010546E-4 54-55 0.0 0.0 0.0 1.1620721358916004 3.9318969240115047E-4 56-57 0.0 0.0 0.0 1.3447742796273348 3.9318969240115047E-4 58-59 0.0 0.0 0.0 1.547791224137129 3.9318969240115047E-4 60-61 0.0 0.0 0.0 1.7778727258072022 3.9318969240115047E-4 62-63 0.0 0.0 0.0 2.002416805975959 5.24252923201534E-4 64-65 0.0 0.0 0.0 2.226272804183014 5.24252923201534E-4 66-67 0.0 0.0 0.0 2.486924804437277 5.24252923201534E-4 68-69 0.0 0.0 0.0 2.7835208957385444 5.24252923201534E-4 70-71 0.0 0.0 0.0 3.1012836988140746 5.24252923201534E-4 72-73 0.0 0.0 0.0 3.432611546277444 5.24252923201534E-4 74-75 0.0 0.0 0.0 3.7557807076234893 5.24252923201534E-4 76-77 0.0 0.0 0.0 4.101591042090302 5.24252923201534E-4 78-79 0.0 0.0 0.0 4.4961241326071555 5.24252923201534E-4 80-81 0.0 0.0 0.0 4.926371953517114 5.24252923201534E-4 82-83 0.0 0.0 0.0 5.3655976057369 5.24252923201534E-4 84-85 0.0 0.0 0.0 5.804430068264283 5.24252923201534E-4 86-87 0.0 0.0 0.0 6.2590556501031145 5.24252923201534E-4 88-89 0.0 0.0 0.0 6.736944955409013 5.24252923201534E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4360 0.0 46.906013 1 GTATCAA 7655 0.0 41.440716 1 TATCAAC 9845 0.0 32.123585 2 ATCAACG 9955 0.0 31.768625 3 TCAACGC 10100 0.0 31.312544 4 CAACGCA 10280 0.0 30.671745 5 AACGCAG 10615 0.0 29.724182 6 GTACATG 8605 0.0 28.519732 1 TACATGG 8565 0.0 28.040262 2 GGACCGA 335 0.0 26.950733 6 ACCGTGC 195 5.329639E-10 26.809649 8 ACATGGG 8585 0.0 26.756227 3 ACGCAGA 12075 0.0 25.977901 7 GTTTACG 110 9.39692E-5 25.9421 1 CGCAGAG 12175 0.0 25.72465 8 CATGGGA 5185 0.0 24.122652 4 GCAGAGT 12910 0.0 23.855131 9 TAGGTCG 140 1.7634173E-5 23.778698 5 TAGGACA 495 0.0 23.058132 4 CATGGGG 3185 0.0 22.994785 4 >>END_MODULE