FastQCFastQC Report
Thu 26 May 2016
SRR938315_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938315_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1525981
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118340.7755011366458692No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86220.5650135879804532No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72550.4754318697283911No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41550.2722838619877967No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28230.1849957502747413No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.16953028904029604No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA24940.1634358488080782No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23050.15105037349744196No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA20920.1370921394172011No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG20060.13145642049278464No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19050.12483772733736527No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA18760.1229373104907597No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG17700.11599095925833938No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16790.11002758225692195No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC15710.10295016779370122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48700.045.9648631
GTATCAA83900.040.0489851
TATCAAC107550.031.2423042
ATCAACG109250.030.6985553
TCAACGC110100.030.4184074
CAACGCA112600.029.7430425
TAAGGTG9050.029.3950445
AACGCAG115350.029.06186
GTACATG86150.026.0939541
TACATGG86000.025.9184132
GTAAGGT9150.025.4395624
ACGCAGA133000.025.2051017
CGCAGAG132800.025.1357968
ACATGGG87600.024.0233923
GGTAAGG8700.023.479233
GCAGAGT141500.023.2883349
GAGTACT87450.022.50609412-13
AGGTAAG8250.022.466982
CATGGGA54100.022.391214
AGTACTT90200.021.398812-13
Produced by FastQC (version 0.11.5)