##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938315_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1525981 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.52114279273464 31.0 31.0 34.0 30.0 34.0 2 31.746420171679727 31.0 31.0 34.0 30.0 34.0 3 31.789895811284676 31.0 31.0 34.0 30.0 34.0 4 35.53606630750973 37.0 35.0 37.0 33.0 37.0 5 35.43918502261824 37.0 35.0 37.0 33.0 37.0 6 35.4544237444634 37.0 35.0 37.0 33.0 37.0 7 35.38204079867312 37.0 35.0 37.0 33.0 37.0 8 35.35974038995243 37.0 35.0 37.0 32.0 37.0 9 36.87978225154835 39.0 37.0 39.0 33.0 39.0 10-11 36.76287417733248 39.0 36.0 39.0 32.0 39.0 12-13 36.63024736218865 39.0 35.0 39.0 32.0 39.0 14-15 37.52416904273382 39.0 36.0 41.0 32.0 41.0 16-17 37.43046997308616 39.0 36.0 40.5 32.0 41.0 18-19 37.718855280635864 39.5 37.0 41.0 32.0 41.0 20-21 37.687804435310795 39.0 37.0 41.0 32.0 41.0 22-23 37.54875716014813 39.0 36.0 41.0 32.0 41.0 24-25 37.381977560664254 39.0 36.0 41.0 32.0 41.0 26-27 37.126724710202815 39.0 36.0 41.0 31.0 41.0 28-29 36.974430546645074 39.0 36.0 40.0 31.0 41.0 30-31 36.6223668577787 38.5 35.0 40.0 30.0 41.0 32-33 36.322359190579704 38.0 35.0 40.0 30.0 41.0 34-35 36.13407866808302 38.0 35.0 40.0 30.0 41.0 36-37 36.02283514670235 38.0 35.0 40.0 30.0 41.0 38-39 35.796046608706135 38.0 35.0 40.0 29.0 41.0 40-41 35.552015392065826 38.0 34.5 40.0 27.5 41.0 42-43 35.25034846436489 38.0 34.0 40.0 27.0 41.0 44-45 34.84231323981098 38.0 33.5 40.0 25.5 41.0 46-47 34.30733999964613 38.0 33.0 40.0 24.0 41.0 48-49 33.81700427462727 37.0 33.0 40.0 23.0 41.0 50-51 33.35547559242218 37.0 32.0 40.0 21.5 41.0 52-53 32.77502603243422 36.5 31.0 39.0 19.0 40.5 54-55 32.364375768767765 36.0 31.0 39.0 17.5 40.0 56-57 31.967136222534883 35.5 31.0 39.0 14.5 40.0 58-59 31.402104941018273 35.0 29.5 38.5 10.5 40.0 60-61 30.934265891908222 35.0 29.0 38.0 8.0 40.0 62-63 30.383543110956168 34.0 28.0 38.0 7.0 40.0 64-65 29.766475139598725 34.0 28.0 37.5 4.0 39.5 66-67 29.189956821218615 34.0 26.5 37.0 2.0 39.0 68-69 28.619080774924456 33.0 26.0 36.0 2.0 39.0 70-71 27.9660831294754 33.0 25.5 36.0 2.0 39.0 72-73 27.392797158024905 32.0 25.0 35.0 2.0 38.0 74-75 26.739596364568104 32.0 23.5 35.0 2.0 37.5 76-77 25.692861837729303 30.5 22.5 34.0 2.0 36.0 78-79 26.350172774104003 31.5 24.0 34.5 2.0 36.0 80-81 25.882989041147958 31.0 22.0 34.5 2.0 36.0 82-83 25.3371870291963 31.0 20.0 34.0 2.0 35.5 84-85 24.663470252906166 31.0 18.0 34.0 2.0 35.0 86-87 23.916851520431777 30.5 12.5 34.0 2.0 35.0 88-89 23.000049804027704 29.5 4.5 34.0 2.0 35.0 90-91 22.223608288700845 29.0 2.0 34.0 2.0 35.0 92-93 21.22570497273557 29.0 2.0 33.0 2.0 35.0 94-95 20.215233348252696 27.0 2.0 33.0 2.0 34.5 96-97 18.9387367863689 25.5 2.0 33.0 2.0 34.0 98-99 17.412468438335733 22.0 2.0 32.0 2.0 34.0 100-101 13.970694916909189 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19316578790980543 1101 2 0.19509633208663502 1101 3 0.2941070293509398 1101 4 0.18386650169770746 1101 5 0.10149687812945984 1101 6 0.19469461853019965 1101 7 0.23048509126398642 1101 8 0.12119310184095156 1101 9 0.20465540729730947 1101 10-11 0.2276129508425555 1101 12-13 0.28803067593617726 1101 14-15 0.28325330228368273 1101 16-17 0.44770895276545275 1101 18-19 0.3425450926887308 1101 20-21 0.3668030801300688 1101 22-23 0.5247952519226189 1101 24-25 0.5575088444122471 1101 26-27 0.3575084185507791 1101 28-29 0.41318956926007644 1101 30-31 0.6941002440096256 1101 32-33 0.501547935758758 1101 34-35 0.36855756726227895 1101 36-37 0.6364128837653382 1101 38-39 0.6472846336356852 1101 40-41 0.7321134880525193 1101 42-43 0.8340208701433482 1101 44-45 0.876401588179057 1101 46-47 0.9608124274673955 1101 48-49 0.7240395849719192 1101 50-51 0.7720697280812914 1101 52-53 0.8909338899772621 1101 54-55 0.946003314093673 1101 56-57 0.8090678783452354 1101 58-59 0.85879619607433 1101 60-61 0.9531594179912837 1101 62-63 1.0150599504478883 1101 64-65 0.763957203750703 1101 66-67 0.7574064949973582 1101 68-69 1.123749481231247 1101 70-71 1.016296921819606 1101 72-73 1.0007539082023982 1101 74-75 1.2657851126816801 1101 76-77 1.0589498219034148 1101 78-79 1.3405801207322732 1101 80-81 1.3925331985187128 1101 82-83 1.019588446292783 1101 84-85 1.0840781326262778 1101 86-87 1.2921260108368493 1101 88-89 1.3022003817885484 1101 90-91 1.131396433560628 1101 92-93 1.4635816790535685 1101 94-95 1.7700076177853177 1101 96-97 1.733492405244423 1101 98-99 1.680959244293419 1101 100-101 1.3572136042605916 1102 1 0.3536776534263879 1102 2 0.35107090574813427 1102 3 0.2992148254864233 1102 4 0.30946739505554177 1102 5 0.2609374273694911 1102 6 0.2941900788072189 1102 7 0.23594296957153915 1102 8 0.296438402474017 1102 9 0.22115870228368095 1102 10-11 0.38690763251089066 1102 12-13 0.32444327370797055 1102 14-15 0.4946725451337386 1102 16-17 0.5289069469242662 1102 18-19 0.4144253905178985 1102 20-21 0.3918453006883169 1102 22-23 0.45593724914140665 1102 24-25 0.5156655159087506 1102 26-27 0.5552335327326716 1102 28-29 0.5614335668095052 1102 30-31 0.5875618111459815 1102 32-33 0.5866794921009202 1102 34-35 0.736230558098562 1102 36-37 0.9070677319171949 1102 38-39 0.9555992472973216 1102 40-41 0.7715844425774847 1102 42-43 0.9184194419377434 1102 44-45 0.9336720331399775 1102 46-47 0.7527906834747071 1102 48-49 0.8568224302971856 1102 50-51 0.9395435286446627 1102 52-53 1.0768469723535503 1102 54-55 0.820920655803036 1102 56-57 1.0758981598048116 1102 58-59 1.123989114041649 1102 60-61 1.0436767292305404 1102 62-63 1.1141153768836283 1102 64-65 1.2527829373835466 1102 66-67 0.8751832138846147 1102 68-69 1.0303796485899746 1102 70-71 1.1284062803588562 1102 72-73 1.2669346737802485 1102 74-75 1.439344832486281 1102 76-77 1.3880096405738165 1102 78-79 1.5443781881490217 1102 80-81 1.5812445071453176 1102 82-83 1.654143987801831 1102 84-85 1.4293466748257693 1102 86-87 1.2969303213856698 1102 88-89 1.4097631291556354 1102 90-91 1.5857265124263584 1102 92-93 1.954455636505621 1102 94-95 2.033730514780121 1102 96-97 2.1783767484675742 1102 98-99 2.179450730489485 1102 100-101 2.1440986508895907 1103 1 0.13440109771791242 1103 2 0.18854242751600125 1103 3 0.21654203162605867 1103 4 0.18926009685249312 1103 5 0.030393211269277742 1103 6 0.07297039403111683 1103 7 0.1945704159780277 1103 8 -0.06559710103667982 1103 9 0.274891391795137 1103 10-11 0.19493207908066523 1103 12-13 0.3996125879754686 1103 14-15 0.4183493278905104 1103 16-17 0.4351589394285611 1103 18-19 0.35990658516516305 1103 20-21 0.3031351216156395 1103 22-23 0.2722788946194967 1103 24-25 0.36123807416763043 1103 26-27 0.485671222749545 1103 28-29 0.5687687040881855 1103 30-31 0.5042770754784058 1103 32-33 0.5106750755450591 1103 34-35 0.6344386649667726 1103 36-37 0.6410211714655816 1103 38-39 0.594407788045487 1103 40-41 0.6824123912894606 1103 42-43 0.7822655000838807 1103 44-45 0.8467172017544371 1103 46-47 0.9437200263565089 1103 48-49 1.0100197679226568 1103 50-51 1.0568664581897167 1103 52-53 1.0371422906488164 1103 54-55 0.932019585099269 1103 56-57 0.8009539499251055 1103 58-59 1.057100842019132 1103 60-61 0.8108367593999724 1103 62-63 1.0088559431047521 1103 64-65 1.0404787859765499 1103 66-67 1.0941378586551167 1103 68-69 1.0805732616903505 1103 70-71 0.8808878715630684 1103 72-73 0.8163627764496226 1103 74-75 1.044089131008132 1103 76-77 0.9783153782086274 1103 78-79 1.4939614145674902 1103 80-81 1.3494966240596433 1103 82-83 1.660898285779389 1103 84-85 1.9210644662368104 1103 86-87 1.9696176556676264 1103 88-89 1.7067772885715335 1103 90-91 1.9256807917424155 1103 92-93 2.2880576177352587 1103 94-95 1.9438994791444237 1103 96-97 1.981994358706519 1103 98-99 2.1405951472008624 1103 100-101 1.958506498051877 1104 1 0.33509823635029434 1104 2 0.34504426277919364 1104 3 0.29879132123171104 1104 4 0.20011111380860314 1104 5 0.08581031823730711 1104 6 0.1540591550510939 1104 7 0.26436866771714307 1104 8 0.2980224935251172 1104 9 0.3163993321556191 1104 10-11 0.339950730432939 1104 12-13 0.3806864129776173 1104 14-15 0.580942444626686 1104 16-17 0.6160118980669935 1104 18-19 0.48573880140882864 1104 20-21 0.40653063856989036 1104 22-23 0.5499245301283793 1104 24-25 0.5282821242182294 1104 26-27 0.5540264684657075 1104 28-29 0.6227971347981338 1104 30-31 0.5436222804565816 1104 32-33 0.6053289146617402 1104 34-35 0.6589171178940418 1104 36-37 0.6408200953255019 1104 38-39 0.622183180209305 1104 40-41 0.7911747928517627 1104 42-43 0.9824555609108145 1104 44-45 1.027755740414456 1104 46-47 1.0378750024775485 1104 48-49 1.1711952698662174 1104 50-51 1.2015158165010433 1104 52-53 1.247176680829881 1104 54-55 1.306686978652138 1104 56-57 1.1739968648383474 1104 58-59 1.1685509799905986 1104 60-61 1.0530885134485644 1104 62-63 1.070465896467038 1104 64-65 1.1121440399448623 1104 66-67 1.2279647651444456 1104 68-69 1.358982501110983 1104 70-71 1.355996811143143 1104 72-73 1.3250355619049259 1104 74-75 1.3929610882612486 1104 76-77 1.1326066828324457 1104 78-79 1.302078020058719 1104 80-81 1.1532552729474652 1104 82-83 1.6126874340156334 1104 84-85 1.8146175208391604 1104 86-87 1.623223957173554 1104 88-89 1.7616598728013386 1104 90-91 1.749699028620192 1104 92-93 2.212878665571587 1104 94-95 2.191806756345713 1104 96-97 2.098489883502179 1104 98-99 1.814467011103865 1104 100-101 1.8186153816218837 1105 1 0.06804752435451533 1105 2 0.13126758381862302 1105 3 0.15000250573180196 1105 4 0.1442398177772759 1105 5 -0.02333755289312478 1105 6 0.137433694029653 1105 7 -0.07864363441949962 1105 8 0.042014507086662434 1105 9 0.21201674137125082 1105 10-11 0.1109280232656289 1105 12-13 0.12405253409315975 1105 14-15 0.21431327945499845 1105 16-17 0.18938167249150695 1105 18-19 0.20645731213706142 1105 20-21 0.19265808748529878 1105 22-23 0.1591846152221521 1105 24-25 0.13225217434772674 1105 26-27 0.05147133109983315 1105 28-29 0.09125175605240088 1105 30-31 0.21949333680759509 1105 32-33 0.31342190190638775 1105 34-35 0.20835699227250615 1105 36-37 0.42265799299508444 1105 38-39 0.12948253510611352 1105 40-41 0.3426632075771394 1105 42-43 0.3372712104371871 1105 44-45 0.41159377027135946 1105 46-47 0.2420973342909747 1105 48-49 0.3324826872309785 1105 50-51 0.34885329509323526 1105 52-53 0.4261169161814138 1105 54-55 0.35110863732704445 1105 56-57 0.29353415110524494 1105 58-59 0.22770753045347192 1105 60-61 0.19849983391276282 1105 62-63 0.19225309085648234 1105 64-65 0.1741219181785496 1105 66-67 0.29607791458729693 1105 68-69 0.421660717947578 1105 70-71 0.39533543148517225 1105 72-73 0.28104387518016694 1105 74-75 0.2681824293960595 1105 76-77 0.05898034153058873 1105 78-79 0.4090448003677416 1105 80-81 0.6597119336632851 1105 82-83 0.6261690883036621 1105 84-85 0.6957547893842424 1105 86-87 0.7925136101019383 1105 88-89 0.8895345839139353 1105 90-91 0.9857895812939326 1105 92-93 1.207789816806958 1105 94-95 1.38440967724976 1105 96-97 1.5358312768727664 1105 98-99 1.9342149255220207 1105 100-101 1.5673191106117255 1106 1 0.024814035561480097 1106 2 0.12091893077110072 1106 3 0.17926505746047283 1106 4 0.08251538286963722 1106 5 -0.010761738919271124 1106 6 -0.036879327362477454 1106 7 0.06257289247940889 1106 8 0.15986208359400678 1106 9 0.026760962722512716 1106 10-11 0.21957702007615154 1106 12-13 -1.0411527588161107E-4 1106 14-15 0.015559511564362083 1106 16-17 0.14636604623706262 1106 18-19 0.20009532458843182 1106 20-21 0.18151169991624272 1106 22-23 0.21847314988674782 1106 24-25 0.32718805634432613 1106 26-27 0.08936627537519826 1106 28-29 0.21305680374860714 1106 30-31 0.33239849909147523 1106 32-33 0.15010491738667042 1106 34-35 0.26485929497371075 1106 36-37 0.29502484869490786 1106 38-39 0.20763643291143552 1106 40-41 0.4557590451983913 1106 42-43 0.4005934864255991 1106 44-45 0.27008730738096887 1106 46-47 -0.5025051851779807 1106 48-49 -0.19604672238708787 1106 50-51 0.026981465412845296 1106 52-53 0.1739539827371459 1106 54-55 0.46271319309911974 1106 56-57 0.30970706900354017 1106 58-59 0.042377853707467494 1106 60-61 0.35643065069657 1106 62-63 0.328951250885094 1106 64-65 0.5953708142687013 1106 66-67 0.33805166890257965 1106 68-69 0.06968791244737105 1106 70-71 0.12064416131373079 1106 72-73 0.39913139242305107 1106 74-75 0.5721758460791442 1106 76-77 0.47368131881011877 1106 78-79 0.7582134923896682 1106 80-81 0.11651037614684512 1106 82-83 0.6381482435662988 1106 84-85 0.9568696106055867 1106 86-87 1.1605080704816082 1106 88-89 1.492397633405588 1106 90-91 1.3784358995265116 1106 92-93 1.6151996976211898 1106 94-95 1.7613569377286886 1106 96-97 2.3668454872031717 1106 98-99 2.036048328191839 1106 100-101 1.3662361841740882 1107 1 0.0896055293050999 1107 2 0.20675545688225228 1107 3 0.23296309122238057 1107 4 0.13502682098472008 1107 5 0.0909989154306956 1107 6 0.05600604595890957 1107 7 0.10891168789060401 1107 8 0.0897539963839904 1107 9 0.1278528286056897 1107 10-11 0.20020285949728844 1107 12-13 0.2903932251447827 1107 14-15 0.37725973675367896 1107 16-17 0.3212729197334383 1107 18-19 0.26747218963791397 1107 20-21 0.33407370943319137 1107 22-23 0.4187829913380696 1107 24-25 0.40519261143509056 1107 26-27 0.39275510898978894 1107 28-29 0.42575260760485634 1107 30-31 0.3238891278075968 1107 32-33 0.39236996650139844 1107 34-35 0.550948128405075 1107 36-37 0.5238904918403762 1107 38-39 0.4062142038486414 1107 40-41 0.418236164387487 1107 42-43 0.3713828217231523 1107 44-45 0.2837087877671465 1107 46-47 0.2029110852544207 1107 48-49 0.3673798931951353 1107 50-51 0.598494191632831 1107 52-53 0.5417914872949865 1107 54-55 0.5148449792407206 1107 56-57 0.4002520491506054 1107 58-59 0.4494541853752452 1107 60-61 0.5439457442445033 1107 62-63 0.5547969018361449 1107 64-65 0.5112625108476188 1107 66-67 0.5633606400127498 1107 68-69 0.8542575882956776 1107 70-71 0.8834714324918771 1107 72-73 0.7892496305593859 1107 74-75 0.7550477153703916 1107 76-77 1.0642961094793257 1107 78-79 1.3389025460631494 1107 80-81 1.3157796560357333 1107 82-83 1.4346362795819303 1107 84-85 1.52581162389707 1107 86-87 1.7201421973828452 1107 88-89 1.7927666580554842 1107 90-91 2.000028239058505 1107 92-93 1.968071365359748 1107 94-95 2.033135530267945 1107 96-97 2.0138735928094498 1107 98-99 1.624126853119325 1107 100-101 1.4839568797873852 1108 1 0.23956953086134547 1108 2 0.16294765818239654 1108 3 0.16623259406867774 1108 4 0.1255670527629249 1108 5 0.17072074256837766 1108 6 0.24340767866372914 1108 7 0.3032650158988943 1108 8 0.2312532049434779 1108 9 0.3453832265620136 1108 10-11 0.42821726487766654 1108 12-13 0.3656382265385716 1108 14-15 0.47383142430594916 1108 16-17 0.3318803611126242 1108 18-19 0.3154002062568537 1108 20-21 0.4988385302307279 1108 22-23 0.34285985354938475 1108 24-25 0.5054044751264826 1108 26-27 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4 41.0 5 129.0 6 247.0 7 615.0 8 1278.0 9 2062.0 10 3366.0 11 5172.0 12 7055.0 13 10107.0 14 12538.0 15 16430.0 16 20300.0 17 25016.0 18 22982.0 19 19775.0 20 19891.0 21 20943.0 22 22585.0 23 25324.0 24 28845.0 25 33219.0 26 38491.0 27 45118.0 28 52574.0 29 61634.0 30 71735.0 31 83602.0 32 97274.0 33 116200.0 34 139997.0 35 167092.0 36 175336.0 37 139194.0 38 39116.0 39 679.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.150342011069654 19.992870127538215 14.680328760516234 22.1764591008759 2 16.437024264556438 21.852196660263427 38.34862024428462 23.362158830895513 3 19.61354695766199 25.924962368469856 30.093756082153057 24.367734591715102 4 13.386339672643368 17.24870755271527 35.93006728130953 33.43488549333183 5 13.06163845608183 38.6235883234534 33.56279428366881 14.751978936795965 6 31.95242928974869 36.72647300326806 16.642343515417295 14.678754191565952 7 28.23645903848082 33.91483904452283 20.176725660411236 17.671976256585108 8 27.281794465330826 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30.811769004051552 21.44931952560933 32-33 20.809076959724397 26.65875259301685 30.802631893673123 21.729538553585627 34-35 21.611739496737336 26.71947312385738 30.575358159053128 21.09342922035216 36-37 21.826273251437193 26.33257113529709 30.51595355759238 21.32520205567334 38-39 21.30984941250331 26.611819006654002 30.58698552057164 21.491346060271045 40-41 21.58204808105035 26.583178513268653 30.569639539570044 21.26513386611095 42-43 21.40922057436743 26.632369064134835 30.254915439950143 21.70349492154759 44-45 21.343010920053413 26.89721387084354 30.37586938534763 21.383905823755416 46-47 21.874977473507208 27.312168369068818 29.423957441147696 21.388896716276285 48-49 21.635819843104205 27.41744490920922 29.185061937206296 21.761673310480276 50-51 21.841408727415228 27.637579432925747 29.04055314215913 21.48045869749989 52-53 22.244674081787387 27.348898839500624 28.89652623459925 21.50990084411274 54-55 21.4617238935397 27.647409808327026 29.16638951845676 21.72447677967651 56-57 21.677104760806326 27.913912427481076 28.685055711702834 21.723927100009767 58-59 21.571733829737028 27.33508560341889 28.927794237172595 22.16538632967148 60-61 21.252427127205383 27.68877856277372 29.180245363474384 21.878548946546516 62-63 21.481841694877822 27.61408518364748 29.269561872130485 21.634511249344214 64-65 21.119763316556895 27.625444780963605 29.120970590876233 22.13382131160327 66-67 21.051846875998397 27.805719622187087 28.9859507991251 22.156482702689416 68-69 21.632138488052803 27.344272545452696 29.0895958635005 21.933993102994002 70-71 21.386078856814077 27.561385102435743 28.741412900947 22.311123139803183 72-73 21.324544669953298 27.66305740372914 28.718509601364627 22.29388832495293 74-75 21.329175529038537 28.037289146557686 28.384453788397696 22.24908153600608 76-77 21.264279455253966 27.636915090204756 28.936887651621124 22.16191780292016 78-79 21.378995924676325 27.7347178639719 28.789831421748474 22.096454789603303 80-81 21.43683286122137 27.971642474410746 28.43057922123976 22.160945443128124 82-83 21.41803835089135 28.01149681097553 28.364306918572126 22.206157919561 84-85 21.293413385582927 27.951664538210135 28.373980462768067 22.380941613438868 86-87 21.46833171619183 27.87771723191251 28.21051741646653 22.44343363542913 88-89 21.262130541730574 27.984040301288992 27.94406563060453 22.809763526375903 90-91 21.18942111902372 27.961631449940665 28.17891951787612 22.670027913159498 92-93 21.324809994657894 27.653307027002228 28.232188330607656 22.789694647732226 94-95 21.064702911449118 27.396948243635784 28.445729346960057 23.09261949795504 96-97 21.288684529334947 26.982672267761714 28.3595753551818 23.36906784772154 98-99 20.97602602194044 27.313432004010036 28.022563975466415 23.68797799858311 100-101 21.121371473962895 27.026831291255686 28.458169932712863 23.393627302068555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3142.0 1 2146.5 2 2041.0 3 2658.5 4 1935.0 5 1588.5 6 2537.0 7 4781.5 8 6763.0 9 6753.0 10 4880.5 11 3300.5 12 2964.5 13 2997.0 14 2976.0 15 2938.5 16 3031.0 17 3111.0 18 3277.5 19 3557.0 20 3919.0 21 4423.0 22 5174.0 23 6120.5 24 7101.5 25 8488.0 26 10322.5 27 12636.5 28 14814.5 29 16685.5 30 19043.0 31 22465.0 32 27933.5 33 29825.5 34 28494.0 35 29537.5 36 32474.0 37 35654.5 38 38035.5 39 39672.5 40 41187.5 41 43904.0 42 46276.5 43 47891.5 44 49605.0 45 52340.5 46 54023.5 47 54811.0 48 55931.5 49 54506.5 50 52711.5 51 51132.0 52 48952.0 53 48830.5 54 49276.5 55 46352.0 56 41879.0 57 38135.5 58 35033.5 59 30888.0 60 25121.0 61 21105.5 62 18513.5 63 14355.0 64 10240.5 65 7865.5 66 6070.5 67 4904.5 68 3877.0 69 2841.0 70 2123.5 71 1532.0 72 1183.0 73 1015.0 74 815.0 75 644.5 76 482.5 77 337.0 78 220.5 79 143.5 80 107.5 81 69.5 82 47.5 83 31.0 84 12.0 85 5.0 86 6.5 87 7.0 88 3.0 89 1.5 90 2.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.5872130780134224E-4 2 0.04659297854953633 3 0.0 4 0.0 5 0.043709587471927894 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006323800886118504 26-27 0.002031480077405944 28-29 1.3106323080038347E-4 30-31 0.0015399929619045061 32-33 4.91487115501438E-4 34-35 0.010321229425530199 36-37 0.022903299582367018 38-39 0.003768067885511025 40-41 0.0010485058464030678 42-43 0.001572758769604602 44-45 0.007765496424922721 46-47 0.0 48-49 0.0 50-51 5.242529232015339E-4 52-53 0.0 54-55 0.0014744613465043142 56-57 0.0 58-59 0.022346280851465387 60-61 0.0 62-63 0.008945065502126174 64-65 0.0150722715420441 66-67 0.006127206039917928 68-69 0.016153543196147264 70-71 0.0 72-73 0.0 74-75 0.022018622774464425 76-77 0.04957466705024506 78-79 0.030013479853287818 80-81 0.025524564198374684 82-83 0.033716016123398654 84-85 0.014908442503543623 86-87 0.00920719196372694 88-89 0.08293025928894265 90-91 0.023362020890168356 92-93 0.02398457123647018 94-95 0.026245411967776792 96-97 0.03702536270110834 98-99 0.052851247820254646 100-101 0.013532278580139596 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1525981.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.13610125745161 #Duplication Level Percentage of deduplicated Percentage of total 1 76.34901961075458 40.56889236944213 2 13.175159356034566 14.001532032506269 3 4.481267493464941 7.143512498834384 4 1.979159982632759 4.206593809674818 5 1.0484919492893892 2.7856387192531895 6 0.6427765519341122 2.049278396969195 7 0.4221475760437209 1.5701893442372832 8 0.3086345818039869 1.3119710712230301 9 0.2338818319885725 1.1184811836140764 >10 1.2268359587298199 11.873176979325928 >50 0.08211336111415513 2.9408719514341732 >100 0.04310183466942593 4.34529621222466 >500 0.0039519528213326416 1.469217711634873 >1k 0.0030874631416661262 2.796888118611368 >5k 2.469970513332901E-4 1.0418852832921024 >10k+ 1.2349852566664505E-4 0.7765743177224123 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11834 0.7755011366458692 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8622 0.5650135879804532 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7255 0.4754318697283911 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4155 0.2722838619877967 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2823 0.1849957502747413 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2587 0.16953028904029604 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2494 0.1634358488080782 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2305 0.15105037349744196 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2092 0.1370921394172011 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2006 0.13145642049278464 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1905 0.12483772733736527 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 1876 0.1229373104907597 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 1770 0.11599095925833938 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1679 0.11002758225692195 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1571 0.10295016779370122 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.553161540019175E-5 0.0 20-21 0.0 0.0 0.0 9.829742310028762E-5 0.0 22-23 0.0 0.0 0.0 5.570187309016299E-4 3.2765807700095875E-5 24-25 0.0 0.0 0.0 0.0015727587696046019 6.553161540019175E-5 26-27 0.0 0.0 0.0 0.0029489226930086284 6.553161540019175E-5 28-29 0.0 0.0 0.0 0.005471889885916011 6.553161540019175E-5 30-31 0.0 0.0 0.0 0.01494120831124372 6.553161540019175E-5 32-33 0.0 0.0 0.0 0.035419838123803636 6.553161540019175E-5 34-35 0.0 0.0 0.0 0.06369673016898639 6.553161540019175E-5 36-37 0.0 0.0 0.0 0.10891354479511868 6.553161540019175E-5 38-39 0.0 0.0 0.0 0.18381618119753784 6.553161540019175E-5 40-41 0.0 0.0 0.0 0.2683847308714853 6.553161540019175E-5 42-43 0.0 0.0 0.0 0.3519703063144298 6.553161540019175E-5 44-45 0.0 0.0 0.0 0.4538064366463278 6.553161540019175E-5 46-47 0.0 0.0 0.0 0.5754331148290837 6.553161540019175E-5 48-49 0.0 0.0 0.0 0.7097729263994768 1.310632308003835E-4 50-51 0.0 0.0 0.0 0.8592833069350143 1.9659484620057524E-4 52-53 0.0 0.0 0.0 0.9966703386215163 1.9659484620057524E-4 54-55 0.0 0.0 0.0 1.1513577167736688 1.9659484620057524E-4 56-57 0.0 0.0 0.0 1.330127963585392 3.2765807700095876E-4 58-59 0.0 0.0 0.0 1.5292785427865745 3.9318969240115047E-4 60-61 0.0 0.0 0.0 1.7538226229553318 3.9318969240115047E-4 62-63 6.553161540019175E-5 0.0 0.0 1.9729931106612728 3.9318969240115047E-4 64-65 6.553161540019175E-5 0.0 0.0 2.192327427405715 3.9318969240115047E-4 66-67 6.553161540019175E-5 0.0 0.0 2.447376474543261 3.9318969240115047E-4 68-69 6.553161540019175E-5 0.0 0.0 2.7366330249197075 3.9318969240115047E-4 70-71 6.553161540019175E-5 0.0 0.0 3.0471218186858158 3.9318969240115047E-4 72-73 6.553161540019175E-5 0.0 0.0 3.370847998762763 4.5872130780134224E-4 74-75 6.553161540019175E-5 0.0 0.0 3.687332935337989 4.5872130780134224E-4 76-77 6.553161540019175E-5 3.2765807700095875E-5 0.0 4.026590108264782 4.5872130780134224E-4 78-79 6.553161540019175E-5 6.553161540019175E-5 0.0 4.413030044279712 4.5872130780134224E-4 80-81 6.553161540019175E-5 6.553161540019175E-5 0.0 4.83587279264945 4.5872130780134224E-4 82-83 6.553161540019175E-5 6.553161540019175E-5 0.0 5.267365714252012 4.5872130780134224E-4 84-85 6.553161540019175E-5 6.553161540019175E-5 0.0 5.696008010584666 4.5872130780134224E-4 86-87 6.553161540019175E-5 6.553161540019175E-5 0.0 6.136052807996954 4.5872130780134224E-4 88-89 6.553161540019175E-5 6.553161540019175E-5 0.0 6.600049410838012 4.5872130780134224E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4870 0.0 45.964863 1 GTATCAA 8390 0.0 40.048985 1 TATCAAC 10755 0.0 31.242304 2 ATCAACG 10925 0.0 30.698555 3 TCAACGC 11010 0.0 30.418407 4 CAACGCA 11260 0.0 29.743042 5 TAAGGTG 905 0.0 29.395044 5 AACGCAG 11535 0.0 29.0618 6 GTACATG 8615 0.0 26.093954 1 TACATGG 8600 0.0 25.918413 2 GTAAGGT 915 0.0 25.439562 4 ACGCAGA 13300 0.0 25.205101 7 CGCAGAG 13280 0.0 25.135796 8 ACATGGG 8760 0.0 24.023392 3 GGTAAGG 870 0.0 23.47923 3 GCAGAGT 14150 0.0 23.288334 9 GAGTACT 8745 0.0 22.506094 12-13 AGGTAAG 825 0.0 22.46698 2 CATGGGA 5410 0.0 22.39121 4 AGTACTT 9020 0.0 21.3988 12-13 >>END_MODULE