FastQCFastQC Report
Thu 26 May 2016
SRR938314_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938314_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590079
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122740.7719113327073687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82590.5194081551922891No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69860.43934923988053426No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38780.2438872534006172No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31520.19822914458967134No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA28900.1817519758452253No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26580.1671615058119754No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25250.15879714152567262No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25070.15766512229895496No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22210.13967859458555204No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG21800.13710010634691736No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG19910.12521390446638186No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA19090.1200569279891125No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18450.1160319707385608No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17870.11238435323024831No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA16270.10232196010386906No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43350.049.5339661
GTATCAA77050.040.5983471
TATCAAC98450.031.5667252
ATCAACG99800.031.139723
GTACATG87300.030.9232031
TCAACGC100600.030.8448524
CAACGCA102800.030.2752915
TACATGG88150.030.241832
AACGCAG105850.029.488076
ACATGGG88300.029.1141453
ACGCAGA121950.025.4359867
CATGGGA55700.025.3377914
CGCAGAG123100.025.1983628
GTGTAAG5550.024.8782981
CATGGGG32350.024.5306854
TAGGACG1000.001650073223.7595124
GCAGAGT130300.023.3670279
GAGTACT82400.022.53889312-13
ATGGGAG21750.022.2833825
AGTACTT83550.021.63173112-13
Produced by FastQC (version 0.11.5)