FastQCFastQC Report
Thu 26 May 2016
SRR938314_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938314_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1590079
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119270.7500885176145335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88560.5569534595450918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74440.46815284020479486No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43850.2757724616198315No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.1749598604849193No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA26950.16948843422245058No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26430.16621815645637733No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA25130.15804246204119418No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA22280.14011882428483113No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA20200.1270377132205381No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG19820.12464789485302304No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG19020.11961669828983339No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18200.11445972181256404No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17230.1083593959796966No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC16380.10301374963130763No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.046.0883641
GTATCAA83700.039.9916231
TATCAAC109450.030.4525782
ATCAACG111300.029.8952143
TCAACGC112600.029.5922784
CAACGCA115500.028.8081135
AACGCAG119050.027.9271016
GTACATG87600.026.2159041
TACATGG86550.026.1493972
GTAGGAC2950.025.780823
ACATGGG89250.024.5522633
GTGTACG1551.3991394E-624.5403121
ACGCAGA137100.024.2503417
CGCAGAG137100.024.1810538
GCAGAGT144300.022.7111959
CATGGGA55200.022.5611384
AGTACTT93400.021.99360812-13
TAAGGTG8300.021.7622745
GAGTACT90950.021.51551612-13
TGTACGC1353.771632E-421.1319352
Produced by FastQC (version 0.11.5)