##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938314_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1590079 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.556061051054694 31.0 31.0 34.0 30.0 34.0 2 31.780588260080158 31.0 31.0 34.0 30.0 34.0 3 31.830679481962846 31.0 31.0 34.0 30.0 34.0 4 35.56325503324049 37.0 35.0 37.0 33.0 37.0 5 35.43720909464247 37.0 35.0 37.0 33.0 37.0 6 35.48145343721916 37.0 35.0 37.0 33.0 37.0 7 35.409409846932135 37.0 35.0 37.0 33.0 37.0 8 35.40564085180674 37.0 35.0 37.0 33.0 37.0 9 36.92157873916956 39.0 37.0 39.0 33.0 39.0 10-11 36.82327953516775 39.0 37.0 39.0 32.0 39.0 12-13 36.699662406710615 39.0 35.0 39.0 32.0 39.0 14-15 37.62189897483081 39.0 36.5 41.0 32.0 41.0 16-17 37.544239940279695 39.0 36.0 41.0 32.0 41.0 18-19 37.69528369345171 39.5 36.5 41.0 32.0 41.0 20-21 37.74696131450072 40.0 37.0 41.0 32.0 41.0 22-23 37.62806313397007 39.0 36.5 41.0 32.0 41.0 24-25 37.488676348785184 39.0 36.0 41.0 32.0 41.0 26-27 37.24087891230562 39.0 36.0 41.0 31.0 41.0 28-29 37.08614571980386 39.0 36.0 40.5 31.0 41.0 30-31 36.76286366903783 39.0 35.5 40.0 30.5 41.0 32-33 36.45486922347884 38.0 35.0 40.0 30.0 41.0 34-35 36.22256252676753 38.0 35.0 40.0 30.0 41.0 36-37 36.14522014315012 38.0 35.0 40.0 30.0 41.0 38-39 35.965894776297276 38.0 35.0 40.0 30.0 41.0 40-41 35.72982851795414 38.0 35.0 40.0 29.0 41.0 42-43 35.43077042084072 38.0 34.0 40.0 27.5 41.0 44-45 35.06901701110448 38.0 34.0 40.0 26.5 41.0 46-47 34.56297171398403 38.0 33.0 40.0 24.5 41.0 48-49 34.08710416274914 38.0 33.0 40.0 23.5 41.0 50-51 33.64734142140107 37.0 33.0 40.0 22.5 41.0 52-53 33.08941914206778 37.0 32.0 40.0 20.0 41.0 54-55 32.71947242872838 36.0 31.0 39.0 19.0 40.5 56-57 32.33651818557443 36.0 31.0 39.0 17.5 40.0 58-59 31.836253104405504 35.0 30.5 39.0 13.5 40.0 60-61 31.424534881600223 35.0 30.0 38.5 11.5 40.0 62-63 30.908475931070093 35.0 29.0 38.0 8.0 40.0 64-65 30.349996446717427 34.0 28.0 38.0 7.0 40.0 66-67 29.806248620351568 34.0 28.0 37.0 4.0 39.5 68-69 29.214394064697416 34.0 26.5 36.5 2.0 39.0 70-71 28.579848862855243 33.0 26.0 36.0 2.0 39.0 72-73 28.094444678534842 33.0 26.0 35.5 2.0 38.0 74-75 27.557407525034918 32.0 25.5 35.0 2.0 38.0 76-77 26.4388379445298 30.5 24.0 34.0 2.0 36.0 78-79 27.11520213775542 32.0 25.0 35.0 2.0 36.0 80-81 26.796374268196736 32.0 25.0 35.0 2.0 36.0 82-83 26.254887335786464 32.0 23.5 35.0 2.0 36.0 84-85 25.664277372382127 31.0 21.5 34.0 2.0 35.0 86-87 24.998936530826455 31.0 19.5 34.0 2.0 35.0 88-89 24.236292976638268 31.0 17.0 34.0 2.0 35.0 90-91 23.57603270026206 30.0 9.0 34.0 2.0 35.0 92-93 22.70640106560743 29.5 2.0 34.0 2.0 35.0 94-95 21.79034783806339 29.0 2.0 34.0 2.0 35.0 96-97 20.626083358122457 29.0 2.0 33.5 2.0 35.0 98-99 19.230053664000344 26.5 2.0 33.0 2.0 34.5 100-101 15.86114777944995 13.0 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.39782288265729804 1101 2 0.4079596312798728 1101 3 0.3837335833395663 1101 4 0.2865608690632797 1101 5 0.15703744026593824 1101 6 0.3203232794351365 1101 7 0.34465419341589154 1101 8 0.30799811226548 1101 9 0.3697658442232452 1101 10-11 0.4494512586660946 1101 12-13 0.44317261893125703 1101 14-15 0.5902013434117208 1101 16-17 0.530623622501011 1101 18-19 0.5668531344586825 1101 20-21 0.5799402634652893 1101 22-23 0.679204914026954 1101 24-25 0.7811415885527708 1101 26-27 0.7438982181782521 1101 28-29 0.7298757390897634 1101 30-31 0.6085990652417124 1101 32-33 0.61731206169177 1101 34-35 0.6260615403148577 1101 36-37 0.8898539738436142 1101 38-39 0.9176404798170452 1101 40-41 0.7526490289088983 1101 42-43 0.7738317462980362 1101 44-45 0.7353805369035697 1101 46-47 0.7496765836816692 1101 48-49 0.8348520754443527 1101 50-51 0.8271937792041868 1101 52-53 0.9951609637341008 1101 54-55 0.9686342910988657 1101 56-57 0.801722624077911 1101 58-59 0.9369943633558115 1101 60-61 0.7643675884776009 1101 62-63 0.7474347649143311 1101 64-65 0.7942607702798092 1101 66-67 1.002127461322008 1101 68-69 0.9259481448670392 1101 70-71 0.8305937021481604 1101 72-73 0.6025884811038402 1101 74-75 0.7799825945769747 1101 76-77 0.8483790251716101 1101 78-79 0.7468632567275257 1101 80-81 0.8385378112717596 1101 82-83 0.8710529431000538 1101 84-85 1.0429643993523463 1101 86-87 1.2897092626147924 1101 88-89 1.1271014982431176 1101 90-91 1.2724441830788713 1101 92-93 1.150869392535963 1101 94-95 1.0285677379525495 1101 96-97 1.2328196962256932 1101 98-99 1.0143340365304425 1101 100-101 0.7344580605637763 1102 1 0.31349967995827654 1102 2 0.32149988135292773 1102 3 0.310986774191111 1102 4 0.29132251133905385 1102 5 0.27234001144705644 1102 6 0.24074737216709963 1102 7 0.25403121566029796 1102 8 0.3107946143537035 1102 9 0.39697388026855407 1102 10-11 0.42923353396997044 1102 12-13 0.3976223305974287 1102 14-15 0.5287090676774469 1102 16-17 0.5130749772584551 1102 18-19 0.4872910077126207 1102 20-21 0.4776944536878389 1102 22-23 0.41321931976931126 1102 24-25 0.46064364732499996 1102 26-27 0.5037059855600177 1102 28-29 0.4415143526408869 1102 30-31 0.333900971937382 1102 32-33 0.44848254524839604 1102 34-35 0.396535242925907 1102 36-37 0.2887083405323594 1102 38-39 0.45038451020819537 1102 40-41 0.48165846233688825 1102 42-43 0.5482697086534287 1102 44-45 0.6223177724780058 1102 46-47 0.4753222483294621 1102 48-49 0.667679619574109 1102 50-51 0.9043903755285285 1102 52-53 0.9491032501529943 1102 54-55 0.8316375916282652 1102 56-57 0.9223227676973806 1102 58-59 1.0684101402034862 1102 60-61 1.1557406419316756 1102 62-63 1.2347717866452683 1102 64-65 0.8698008723538351 1102 66-67 1.0805782739197483 1102 68-69 1.0769347169754155 1102 70-71 0.8461262381492993 1102 72-73 0.7433814312153295 1102 74-75 0.916897932335683 1102 76-77 0.8743766405749867 1102 78-79 0.8901431191186298 1102 80-81 0.6925932112353621 1102 82-83 0.6691052456553734 1102 84-85 0.5554170830806839 1102 86-87 0.44004531857654783 1102 88-89 0.5518081241446104 1102 90-91 0.3221246637434767 1102 92-93 0.7122784188156039 1102 94-95 0.5739273642701015 1102 96-97 0.7483480791928159 1102 98-99 0.7225477184367293 1102 100-101 0.3070902837281917 1103 1 0.31148350355679355 1103 2 0.26459576206811874 1103 3 0.2708166987717213 1103 4 0.2870214329122902 1103 5 0.3612224799635726 1103 6 0.15748721639434393 1103 7 0.2204104095421613 1103 8 0.2325800391457662 1103 9 0.48141246398172655 1103 10-11 0.3825409883894011 1103 12-13 0.4433457017970923 1103 14-15 0.5847455189132944 1103 16-17 0.5255329062796505 1103 18-19 0.49582304626813567 1103 20-21 0.4718047002105621 1103 22-23 0.4028654903557509 1103 24-25 0.6068742564951464 1103 26-27 0.5159770548236793 1103 28-29 0.5197546256543788 1103 30-31 0.6976104575113666 1103 32-33 0.8352649890950161 1103 34-35 0.6793640059657093 1103 36-37 0.8319254414674688 1103 38-39 0.7770689646511286 1103 40-41 0.8432319096020606 1103 42-43 0.8058164199216975 1103 44-45 0.9700448749990258 1103 46-47 0.9943808422630198 1103 48-49 1.102424814818697 1103 50-51 1.1545298779015312 1103 52-53 1.0257550316717285 1103 54-55 1.136535813633813 1103 56-57 1.1943065061260256 1103 58-59 1.1929998930933827 1103 60-61 1.2521957030777706 1103 62-63 1.051029540040318 1103 64-65 1.1292437145604985 1103 66-67 1.1905764457044405 1103 68-69 1.1455550164796655 1103 70-71 0.7803859284246641 1103 72-73 0.6781670087257865 1103 74-75 0.7128962700547739 1103 76-77 0.7393732190957323 1103 78-79 0.8381860109587684 1103 80-81 0.7827932429537974 1103 82-83 0.9440835126541067 1103 84-85 0.4782570356792455 1103 86-87 0.6160347208015367 1103 88-89 0.6863043810756757 1103 90-91 0.7262979139431849 1103 92-93 0.4782106911477335 1103 94-95 0.9974180220164435 1103 96-97 0.6842309530004371 1103 98-99 0.8014799966426445 1103 100-101 0.306695271745669 1104 1 0.24743827542596364 1104 2 0.3218683562317963 1104 3 0.2810127877950599 1104 4 0.2869972665109799 1104 5 0.15749722920161702 1104 6 0.2732194014919216 1104 7 0.17426182313448635 1104 8 0.17874369401543788 1104 9 0.23967737322978167 1104 10-11 0.35567185940336543 1104 12-13 0.428768030983278 1104 14-15 0.7084780267034034 1104 16-17 0.5485332787265378 1104 18-19 0.508813553137081 1104 20-21 0.4391958732192549 1104 22-23 0.5468931551990437 1104 24-25 0.5532843731193609 1104 26-27 0.5612684447613603 1104 28-29 0.4512030744557265 1104 30-31 0.5319643474490476 1104 32-33 0.6384364028010623 1104 34-35 0.6293880657505397 1104 36-37 0.8187654144010921 1104 38-39 0.6979929456819249 1104 40-41 0.7840336441810436 1104 42-43 0.9058187654841774 1104 44-45 0.6475902722946714 1104 46-47 0.6041968790869277 1104 48-49 0.5963206661583698 1104 50-51 0.7473615546517465 1104 52-53 0.8666651241295327 1104 54-55 0.7090481669295414 1104 56-57 0.7281547496551184 1104 58-59 0.7799031606751505 1104 60-61 0.6847190236550382 1104 62-63 0.7803104047994509 1104 64-65 0.9333288314690193 1104 66-67 0.7831648591944109 1104 68-69 0.8385363472238758 1104 70-71 0.6951600934044215 1104 72-73 0.45743607459486313 1104 74-75 0.48347538232334486 1104 76-77 0.4333409299404778 1104 78-79 0.5395143456378371 1104 80-81 0.5120407012170034 1104 82-83 0.443574596673578 1104 84-85 0.5836319631800428 1104 86-87 0.5534058925592618 1104 88-89 0.4599119343554854 1104 90-91 0.6125377782701626 1104 92-93 0.6564076928081057 1104 94-95 0.644818499089638 1104 96-97 0.70850077229262 1104 98-99 0.6315877077148322 1104 100-101 0.1747339266153407 1105 1 0.2795292625843864 1105 2 0.2536460626182091 1105 3 0.19575819686417262 1105 4 0.29294093872366034 1105 5 0.2552598601871523 1105 6 0.22043776426055217 1105 7 0.19609052887496148 1105 8 0.24689914348433462 1105 9 0.2300095272414211 1105 10-11 0.3279893458998302 1105 12-13 0.43212012842294456 1105 14-15 0.5219110737664536 1105 16-17 0.43345409228798815 1105 18-19 0.35231384677386046 1105 20-21 0.27404087324312343 1105 22-23 0.4565212357421444 1105 24-25 0.558550225242378 1105 26-27 0.42980596308382246 1105 28-29 0.39601061447524444 1105 30-31 0.5481373905696572 1105 32-33 0.4541701161911078 1105 34-35 0.6447373229961428 1105 36-37 0.7165477575884793 1105 38-39 0.5582593537026739 1105 40-41 0.583282772990394 1105 42-43 0.6954481351643551 1105 44-45 0.7413838616683606 1105 46-47 0.6860362798905584 1105 48-49 0.6726104682655958 1105 50-51 0.3930596223785088 1105 52-53 0.45676506294667263 1105 54-55 0.3668593485907792 1105 56-57 0.48930237494866446 1105 58-59 0.7537188427207475 1105 60-61 0.5943351677437256 1105 62-63 0.710401490177837 1105 64-65 0.5988185637309371 1105 66-67 0.5098273856166031 1105 68-69 0.5347365304313989 1105 70-71 0.5210266629424503 1105 72-73 0.6435025490505133 1105 74-75 0.6277676767622538 1105 76-77 0.6429136935434308 1105 78-79 0.8126132606482379 1105 80-81 0.7515557196639406 1105 82-83 0.7707903266014071 1105 84-85 0.6541665185334757 1105 86-87 0.6532773539973498 1105 88-89 0.7618186294786575 1105 90-91 0.9521450825617634 1105 92-93 0.8814461793475381 1105 94-95 0.9883786455192407 1105 96-97 0.9304678385917775 1105 98-99 0.6734918245638575 1105 100-101 0.5073069078028087 1106 1 0.09046328423636396 1106 2 0.16693645876681984 1106 3 0.20631014099895495 1106 4 0.1646781642562587 1106 5 0.06166093403042794 1106 6 -0.011529893587727713 1106 7 0.20565901292622613 1106 8 0.19391949728177593 1106 9 0.10927179084733041 1106 10-11 0.005785519747163903 1106 12-13 0.11267209324037708 1106 14-15 0.381265078720638 1106 16-17 0.2643043009414754 1106 18-19 0.3581396326629047 1106 20-21 0.27034195336013767 1106 22-23 0.31092502039161474 1106 24-25 0.279394580705457 1106 26-27 0.18375703896087714 1106 28-29 0.448939588181382 1106 30-31 0.6036460079964527 1106 32-33 0.4678935725931126 1106 34-35 0.3146480635660609 1106 36-37 -0.048380118558284835 1106 38-39 0.0698130275965454 1106 40-41 0.1774410614596107 1106 42-43 0.36511189040309944 1106 44-45 0.4044400267876185 1106 46-47 0.002091326078364375 1106 48-49 0.07028253241490034 1106 50-51 0.18530859186539317 1106 52-53 0.26743245074846556 1106 54-55 -0.05879823908125559 1106 56-57 0.09074750628695227 1106 58-59 0.22417212371318485 1106 60-61 -0.15706970136611176 1106 62-63 -0.07405236027693718 1106 64-65 -0.507395469999711 1106 66-67 -0.30422988878220636 1106 68-69 -0.9560924371388886 1106 70-71 -1.0016656529698835 1106 72-73 -0.38793301311430284 1106 74-75 -0.016123598674820272 1106 76-77 -0.07254162469018866 1106 78-79 -0.19460015880765624 1106 80-81 -1.043037111315364 1106 82-83 -0.31719574156447905 1106 84-85 -0.14882226250687935 1106 86-87 0.09602967028197185 1106 88-89 -0.24581947375672897 1106 90-91 -0.054907184567092315 1106 92-93 -0.06884427402308191 1106 94-95 -0.11251339724421072 1106 96-97 -0.2808935211133168 1106 98-99 -0.43836616007720863 1106 100-101 -0.8537244601960285 1107 1 0.08919041340911704 1107 2 0.08381861031586268 1107 3 0.0838394836515981 1107 4 0.13394258124819203 1107 5 0.052627524596587705 1107 6 0.11628915049176669 1107 7 0.009458100747806952 1107 8 0.1820861830734728 1107 9 0.18437973310228983 1107 10-11 0.18465726842025987 1107 12-13 0.1565534244111575 1107 14-15 0.2968916576062597 1107 16-17 0.18380275695189852 1107 18-19 0.1811530413089102 1107 20-21 0.12542958311777141 1107 22-23 0.27143416872251436 1107 24-25 0.3834579365149864 1107 26-27 0.3874746027628504 1107 28-29 0.3688881389891847 1107 30-31 0.29885818453362845 1107 32-33 0.26792320212124565 1107 34-35 0.19797263363818018 1107 36-37 0.19825162066076274 1107 38-39 0.13829954102317998 1107 40-41 0.14706411563027189 1107 42-43 0.23980604687783824 1107 44-45 0.219722697624114 1107 46-47 0.2606254008920672 1107 48-49 0.17551000326921695 1107 50-51 0.1662405347232223 1107 52-53 0.30279623455908933 1107 54-55 0.08888834559325431 1107 56-57 0.07293810464928185 1107 58-59 -0.1600887737018546 1107 60-61 -0.010159136298469207 1107 62-63 0.12560938354924644 1107 64-65 0.06627740973351948 1107 66-67 0.04484637214261511 1107 68-69 0.20371793388103399 1107 70-71 0.11858039200191683 1107 72-73 0.05537730738128488 1107 74-75 0.0992274084722311 1107 76-77 0.1645870856374998 1107 78-79 0.2380935420916792 1107 80-81 0.26845075342327007 1107 82-83 0.21595644631070599 1107 84-85 0.3728513091529031 1107 86-87 0.26805990952756886 1107 88-89 0.20722765774528185 1107 90-91 0.419933920665688 1107 92-93 0.3375192237684068 1107 94-95 0.027654480579375473 1107 96-97 0.12756665114899235 1107 98-99 -0.28612090979332905 1107 100-101 -0.3792301745845048 1108 1 0.1739125703915967 1108 2 0.2915996239848937 1108 3 0.2482785299733088 1108 4 0.16082232464350454 1108 5 -0.024630012830520798 1108 6 0.1146373920383752 1108 7 0.10872163724888395 1108 8 0.07171093898363523 1108 9 0.09291264412944855 1108 10-11 0.24679264349659036 1108 12-13 0.26451108866432094 1108 14-15 0.44106856318459364 1108 16-17 0.29478478657149054 1108 18-19 0.1863869649314367 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-0.15646444861476283 2316 86-87 -0.0323015390471042 2316 88-89 -0.11297851468422593 2316 90-91 -0.06337688649222883 2316 92-93 -0.11683032326238774 2316 94-95 -0.2645056380499007 2316 96-97 0.025918893597577153 2316 98-99 0.11701396149853949 2316 100-101 0.28455526552547816 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 12.0 4 36.0 5 100.0 6 255.0 7 578.0 8 1215.0 9 2037.0 10 3362.0 11 5039.0 12 7132.0 13 9722.0 14 12308.0 15 15949.0 16 19538.0 17 24049.0 18 22022.0 19 18581.0 20 17751.0 21 19142.0 22 20421.0 23 23008.0 24 26107.0 25 30750.0 26 35822.0 27 42327.0 28 50554.0 29 58542.0 30 69679.0 31 80998.0 32 96406.0 33 116456.0 34 144440.0 35 178938.0 36 200047.0 37 176287.0 38 59038.0 39 1428.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.32126467467764 19.816750090719335 14.59729106095396 22.26469417364907 2 16.374830120299997 21.68992550460563 38.491468263955305 23.443776111139076 3 19.590331212874943 25.855232333269395 30.00455956203048 24.549876891825175 4 13.253681106410436 17.13656994400907 35.96928202938345 33.64046692019705 5 13.098407059370038 38.59433233649966 33.5630393964362 14.744221207694105 6 31.929356969056254 36.6211364341017 16.761116900481046 14.688389696360998 7 28.364817093993445 33.87070705292001 20.11623321860109 17.648242634485456 8 27.350653646768492 34.70160916533078 19.281117479068648 18.666619708832076 9 27.277135286988884 16.126620123905795 19.7324787007438 36.86376588836152 10-11 25.214533365952256 27.15465709565374 26.64380197461887 20.987007563775133 12-13 26.321333719896934 24.477180064638297 27.591176287467476 21.61030992799729 14-15 22.585513046836038 25.581716380129542 26.513179533847058 25.31959103918736 16-17 21.446796039693623 28.989062807571198 27.334522372787767 22.229618779947412 18-19 21.58861289281853 26.531166061560462 30.77670983642951 21.103511209191492 20-21 22.062142824350236 25.735513770070543 31.156124947251048 21.04621845832817 22-23 21.716656845351707 25.97905512870744 31.061507006884565 21.242781019056288 24-25 21.48914800981336 26.256810204788827 31.189558744639413 21.064483040758404 26-27 21.015646055057584 26.64533899890783 30.892884603228964 21.44613034280562 28-29 21.196871350417183 26.90507830114101 30.853624253889272 21.044426094552534 30-31 21.146244935000087 26.579622773459683 30.741302790616064 21.532829500924166 32-33 20.827770192550183 26.675341288074367 30.710046481967247 21.786842037408206 34-35 21.641156917193687 26.786306186179765 30.430400367315237 21.142136529311315 36-37 21.916195908689986 26.34223803556745 30.40185148208185 21.339714573660718 38-39 21.31694652705408 26.594776540406123 30.56117568406415 21.527101248475645 40-41 21.63141933154801 26.580029459082443 30.577791726048144 21.210759483321404 42-43 21.397270060726807 26.69611816320267 30.162407740026865 21.74420403604365 44-45 21.390572418099982 26.887626338062447 30.333461419212504 21.388339824625067 46-47 21.92441381843292 27.2801540049268 29.324392058507787 21.471040118132496 48-49 21.665181415514574 27.44583759674834 29.06091458348925 21.82806640424784 50-51 21.898345931239895 27.626646223237966 28.975164127065383 21.499843718456756 52-53 22.248391432123814 27.30339184405303 28.893375737935035 21.55484098588812 54-55 21.43006731112102 27.622778490880012 29.164777347540593 21.782376850458373 56-57 21.707349131709808 27.887859659803066 28.64373405346527 21.761057155021856 58-59 21.553583186747325 27.361187714572672 28.885954723004332 22.199274375675675 60-61 21.232498511080266 27.659977900469094 29.20119063266668 21.90633295578396 62-63 21.49757968000332 27.58693121536729 29.23423930509113 21.681249799538264 64-65 21.072789465177515 27.625545648989767 29.0840266427014 22.217638243131315 66-67 21.03072431757979 27.7293035049897 29.02744900243762 22.212523174992892 68-69 21.630143331384765 27.297457668957048 29.105223619412268 21.96717538024592 70-71 21.431697961796818 27.5152712730264 28.66694505989333 22.386085705283453 72-73 21.30466473678352 27.637557630784382 28.678166304944597 22.379611327487503 74-75 21.28403683087444 27.99354623260857 28.418305002455853 22.304111934061137 76-77 21.291174840998465 27.608156575868243 28.873816961295635 22.226851621837657 78-79 21.411892113536496 27.718434115537654 28.79451272546836 22.075161045457488 80-81 21.414079475738106 27.979208135618805 28.477653148181343 22.12905924046175 82-83 21.4302986698984 27.951661141216423 28.356612758019867 22.261427430865314 84-85 21.242485411076355 27.948720448329723 28.405798650998232 22.402995489595693 86-87 21.47211927239612 27.86085390250943 28.202606096320785 22.46442072877366 88-89 21.20668408092315 28.009627209760033 27.96198799240162 22.821700716915192 90-91 21.152627010980993 27.934397051609416 28.213355806845197 22.699620130564398 92-93 21.350627428759022 27.58246381742587 28.29168189144945 22.775226862365656 94-95 21.038320785722956 27.41147126837254 28.429849623634578 23.120358322269926 96-97 21.28803034314647 26.95384946282543 28.391733995606504 23.366386198421587 98-99 21.026416347394715 27.20011496305837 28.09177045625492 23.681698233291993 100-101 21.1286043020504 26.98900494881072 28.469874767228546 23.412515981910335 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 3275.0 1 2206.5 2 2051.5 3 2698.0 4 1974.5 5 1658.5 6 2681.0 7 4928.0 8 6934.5 9 6883.0 10 4931.5 11 3398.5 12 3064.5 13 3027.5 14 3089.5 15 3178.5 16 3205.5 17 3243.0 18 3352.5 19 3612.5 20 3993.5 21 4490.0 22 5311.5 23 6336.5 24 7340.5 25 8697.5 26 10614.5 27 12932.5 28 15085.0 29 17129.5 30 19624.5 31 23247.0 32 28932.0 33 30804.5 34 29598.0 35 31018.0 36 33994.0 37 37090.5 38 39472.0 39 41224.5 40 43074.0 41 46011.5 42 48257.0 43 49864.5 44 51417.5 45 54397.0 46 56447.5 47 57114.5 48 58555.5 49 57099.5 50 55088.0 51 53385.5 52 51392.0 53 51714.5 54 52118.5 55 48645.5 56 43946.5 57 39958.5 58 36506.5 59 32367.0 60 26379.5 61 21987.0 62 19149.0 63 14820.5 64 10452.5 65 7994.5 66 6264.5 67 5096.5 68 3959.5 69 2892.0 70 2155.0 71 1574.0 72 1243.5 73 1043.0 74 857.0 75 688.0 76 498.5 77 318.5 78 224.0 79 160.5 80 104.5 81 63.0 82 38.5 83 22.5 84 12.0 85 10.0 86 4.5 87 5.5 88 5.5 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.288995703987035E-4 2 0.04521787911166678 3 8.804593985581848E-4 4 0.0 5 0.08691392062910082 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.2577991407974068E-4 26-27 0.00789268960850373 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010313952954538737 36-37 9.433493555980551E-5 38-39 0.003773397422392221 40-41 0.0048425266920700165 42-43 4.402296992790924E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.8866987111961102E-4 68-69 0.0014150240333970828 70-71 1.2577991407974068E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.002138258539355592 82-83 7.861244629983792E-4 84-85 0.005565761198028526 86-87 0.002389818367515073 88-89 1.5722489259967586E-4 90-91 0.003710507465352351 92-93 0.0011320192267176663 94-95 1.2577991407974068E-4 96-97 0.0 98-99 1.8866987111961102E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1590079.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.46937719158152 #Duplication Level Percentage of deduplicated Percentage of total 1 76.00731178980863 39.8805631161761 2 13.200885008671357 13.852844295653426 3 4.525827584407724 7.1240206389105944 4 2.0697191363250216 4.343874961778876 5 1.1392657955675338 2.9888283374550064 6 0.6608279926185081 2.080393992207369 7 0.4607720086221123 1.6923494223801385 8 0.3096415471412818 1.2997359304912626 9 0.24145651998641768 1.1402165900280594 >10 1.2470142431894313 11.985234242127198 >50 0.08533220795786248 3.0411480300429887 >100 0.0442506224112 4.354150108292019 >500 0.004088257374400045 1.4917160812696098 >1k 0.0032465573267294475 2.94406357707516 >5k 2.4048572790588498E-4 1.0283781138849029 >10k+ 1.2024286395294249E-4 0.752482562227315 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11927 0.7500885176145335 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8856 0.5569534595450918 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7444 0.46815284020479486 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4385 0.2757724616198315 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2782 0.1749598604849193 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2695 0.16948843422245058 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2643 0.16621815645637733 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2513 0.15804246204119418 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2228 0.14011882428483113 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2020 0.1270377132205381 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 1982 0.12464789485302304 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 1902 0.11961669828983339 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1820 0.11445972181256404 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1723 0.1083593959796966 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 1638 0.10301374963130763 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.144497851993517E-5 0.0 12-13 0.0 0.0 0.0 6.288995703987034E-5 0.0 14-15 0.0 0.0 0.0 9.433493555980551E-5 3.144497851993517E-5 16-17 0.0 0.0 0.0 1.8866987111961105E-4 1.2577991407974068E-4 18-19 0.0 0.0 0.0 1.8866987111961105E-4 1.2577991407974068E-4 20-21 0.0 0.0 0.0 3.1444978519935173E-4 1.2577991407974068E-4 22-23 0.0 0.0 0.0 5.974545918787683E-4 1.5722489259967586E-4 24-25 0.0 0.0 0.0 0.0013835790548771478 3.1444978519935173E-4 26-27 0.0 0.0 0.0 0.00267282317419449 3.1444978519935173E-4 28-29 0.0 0.0 0.0 0.005282756391349109 3.1444978519935173E-4 30-31 0.0 0.0 0.0 0.01525081458216856 3.1444978519935173E-4 32-33 0.0 0.0 0.0 0.03480959122156824 3.1444978519935173E-4 34-35 0.0 0.0 0.0 0.0628585120613504 3.1444978519935173E-4 36-37 0.0 0.0 0.0 0.11427105194144442 3.1444978519935173E-4 38-39 0.0 0.0 0.0 0.19278916330572254 3.1444978519935173E-4 40-41 0.0 0.0 0.0 0.2828475817868169 3.1444978519935173E-4 42-43 0.0 0.0 0.0 0.37293744524643113 3.1444978519935173E-4 44-45 0.0 0.0 0.0 0.4798189272356908 3.1444978519935173E-4 46-47 0.0 0.0 0.0 0.6089948989955845 3.1444978519935173E-4 48-49 0.0 0.0 0.0 0.7507174171849323 3.1444978519935173E-4 50-51 0.0 0.0 0.0 0.9039802424910963 3.1444978519935173E-4 52-53 0.0 0.0 0.0 1.0540042350097072 3.1444978519935173E-4 54-55 0.0 0.0 0.0 1.2155685346451341 3.1444978519935173E-4 56-57 0.0 0.0 0.0 1.4045214105714243 3.1444978519935173E-4 58-59 0.0 0.0 0.0 1.6102030150703204 3.1444978519935173E-4 60-61 0.0 0.0 0.0 1.8411034923422043 3.1444978519935173E-4 62-63 0.0 0.0 0.0 2.069614151246573 3.1444978519935173E-4 64-65 0.0 0.0 0.0 2.3008919682606965 3.1444978519935173E-4 66-67 0.0 0.0 0.0 2.5666020367541487 3.1444978519935173E-4 68-69 0.0 0.0 0.0 2.8665242418772907 3.1444978519935173E-4 70-71 0.0 6.288995703987034E-5 0.0 3.1865397882746707 3.1444978519935173E-4 72-73 0.0 6.288995703987034E-5 0.0 3.514259354409435 4.402296992790924E-4 74-75 0.0 6.288995703987034E-5 0.0 3.840280891704123 4.402296992790924E-4 76-77 0.0 6.288995703987034E-5 0.0 4.19557770400087 4.402296992790924E-4 78-79 0.0 6.288995703987034E-5 0.0 4.5941742517195685 4.402296992790924E-4 80-81 0.0 6.288995703987034E-5 0.0 5.0230837587314845 4.402296992790924E-4 82-83 0.0 6.288995703987034E-5 0.0 5.46680385062629 4.402296992790924E-4 84-85 0.0 6.288995703987034E-5 0.0 5.905775750764585 4.402296992790924E-4 86-87 0.0 6.288995703987034E-5 0.0 6.347892148754873 4.402296992790924E-4 88-89 0.0 6.288995703987034E-5 0.0 6.812554596343955 4.402296992790924E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4890 0.0 46.088364 1 GTATCAA 8370 0.0 39.991623 1 TATCAAC 10945 0.0 30.452578 2 ATCAACG 11130 0.0 29.895214 3 TCAACGC 11260 0.0 29.592278 4 CAACGCA 11550 0.0 28.808113 5 AACGCAG 11905 0.0 27.927101 6 GTACATG 8760 0.0 26.215904 1 TACATGG 8655 0.0 26.149397 2 GTAGGAC 295 0.0 25.78082 3 ACATGGG 8925 0.0 24.552263 3 GTGTACG 155 1.3991394E-6 24.540312 1 ACGCAGA 13710 0.0 24.250341 7 CGCAGAG 13710 0.0 24.181053 8 GCAGAGT 14430 0.0 22.711195 9 CATGGGA 5520 0.0 22.561138 4 AGTACTT 9340 0.0 21.993608 12-13 TAAGGTG 830 0.0 21.762274 5 GAGTACT 9095 0.0 21.515516 12-13 TGTACGC 135 3.771632E-4 21.131935 2 >>END_MODULE